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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 19, Issue 4 - Dec 1981
Volume 19, Issue 3 - Sep 1981
Volume 19, Issue 2 - Jun 1981
Volume 19, Issue 1 - Mar 1981
Selecting the target year
Fungus Flora of Paddy Fields in Korea I. Fungal distribution of paddy fields
Kyung Hee MIN ; Tadayoshi ITO ; Tatsuo YOKOYAMA ;
The Korean Journal of Microbiology, volume 19, issue 4, 1981, Pages 154~162
An investigation of the soil microfungal population in the paddy fields of two locations around Seoul was made at four seasons. By the dilution plate method, a total number of propagules of the microfungi pergram of soil was recorded as
at the upper layer (0.10cm depth) followed by the middle (10-20cm depth) and the lower layer(20-30 cm depth). The highest number of fungal propagules was
at the upper layer of the soil collected in autumn. The decreasing tendency of the number of fungal propagules was depend on the increasing depth in paddy fields. Seasonal fluctuation of the fungal population was shown from the highest density of fungal colonies on the plate in autumn season and the lowest one in winter, indicating that the autumn is best season for fungal growth. Generally, the number of the species of Talaromyces in the paddy soils was found to be very high, particularly in autumn, while comparatively low in winter. The highest number of the species of Talaromyces was
propagules per gram of soil in Yukkog-dong in autumn and the lowest was
in Shinwon-dong in winter. It is assumed that these fungi grow well also in the warm to the hot seasons.
Studies on Methanol-assimilating Yeasts
The Korean Journal of Microbiology, volume 19, issue 4, 1981, Pages 163~173
The distribution of methanol-assimilating yeasts on three different sources (elm bark, soil and fresh-water mud) and the growth conditions of a new strain of Candidaboidinii (SIO) wereexamines. From 150 samples, 91 methanol yeasts were isolated through enrichment culture ; they were identified as 77 strains of Candida boidinii including four new strains, 5 isolates of Torulopsis pinus, 3 strains of Hansenula polymorpha and one sstrain of Pichia pastoris respectively. The comparison of these yeasts with three sources indicated that decaying bark of elm tree other two, and that Gandida boidinii was most frequently distributed in all three sources. Four new strains of Candida boidinii were freshly isolated and their taxonomical properties were discussed. Of them, SIO strain was selected and characterized for its growth on methanol. This yeast could grow well on less than 1%(v/v) methanol. However, its growth was inhibited at 10% methanol. The cell yield was 3.1g (dry weight) per 1000ml of mineral mediurr, containing 1%(v/v) methanol as well as 01.% yeast extract as additive. The concentration of 0.1% yeast extract appears to be effective for the biomass production. Optimum conditions for growth on methanol was found to be :
as nitrogen sources, thiamine as vitamin, and pH 4.5 to 6.0. The cell composition was as follows : crude protein and nucleic acids were 54% and 7% respectively. The amino acids were also described.
On the Psychrophilic Yeasts Isolated from the Han River
The Korean Journal of Microbiology, volume 19, issue 4, 1981, Pages 174~178
Psychrophilic yeast strains, HJ3011, HJ3023, and HJ3021, which were isolated from the Han River during winter season of December 1980 to February 1981 were identified as Candida sp., Candida nivalis, and Rhodotorula rubra respectively. The effect of temperature on growth was carried out in this study. Growth rate constants of strains HJ3011 and HJ3023 were the highest value at
respectively, and both growth rates diminished gradually above
and were decreased to zero at
. According to the results obtained in this experiment, above two strains of yeast were confirmed as psychrophile. On the other hand since the strain of HJ3021 exhibited the highest growth rate constant at
and could slightly grow above
, this strain was defined as psychrotroph.
Growth and Biodegradability of Facultative Psychrophilic SDBS-degrading Pseudomonas spp.
The Korean Journal of Microbiology, volume 19, issue 4, 1981, Pages 179~185
Facultative psychrophilic bacteria utilizing SDBS (Sodium Dodecyl Benzene Sulfonate) as their carbon source were isolated in the Han River. All of these isolated faculatative psychrophilic bacteria were identified as Pseudomonas spp. The growth and biodegradation rates of Ps.fluorescens LP6, Ps. fluorescens LS6 and Ps. putida LC1 among 8 identified facultative psychrophilic bacteria were investigated with spectrophotometer. The specific growth rates of these three facultative psychrophilic bacteria at
were higher than those at any other temperatures. However, the final cell yields were the highest for cells grown at
. The biodegradation of SDBS by Ps. fluorescens LP 6 was started at the stationary phase of cells. The biodegradation rate of SDBS by Ps. fluorescens LP6 was the highest when the cells were cultured at
Formation of Protoplast from Trichoderma koningii
The Korean Journal of Microbiology, volume 19, issue 4, 1981, Pages 186~191
Protoplast production from Trichoderma koningii ATCC 26113 was investigated for the purpose of doing basic and applied researches by protoplast fusion of the cellulolytic filamentous fungus. High yields of protoplast were obtained by using the 18hr. old mycelia treated with the lytic enzyme Driselase of Kyowa Fermentation Co., Japan, in 0.6M
as osmotic stabilizers. The optimum temeprature of mycelial digestion was about
and the number of protoplast increased rapidly after 3hr. digestion. Protoplasts produced at different periods showed distinct morphological heterogeneities in the whole size and the size of vacuole.
Protoplast Reversion of Trichoderma koningii
The Korean Journal of Microbiology, volume 19, issue 4, 1981, Pages 192~198
Protoplasts from Trichoderma koningii were fractioned at varing digestion periods. There were distinct differences between the fractionated protoplasts in CMCase activity and protein content ; namely, protein contents and CMCase activities in the early-protoplasts were higher than the late-produced protoplasts. Reversion frequencies of the early-produced protoplasts. Reversion frequencies of the early-produced protoplasts (0-1hr.) and the late-produced protoplasts (1-2.5 hr.)were 1.25% and 0.56%, respectively. From these results it was assumed that the early-produced protoplasts were more active in physiological metabolism. Meanwhile, in osmotically stabilized liquid medium two distinct reversion patterns of these protoplasts were found. In the first, normal germ tube was developed from the opposite side of the protoplast after the production of an aberrent tube. In the second, reversion occurred through germination of protoplast, but this patterns was uncommon. It is suggested that the the physiological and morphological heterogeneities in protoplast reversion are related to the heterogeneous origins of protoplasts from highly differentiated mycelium.