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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 21, Issue 4 - Dec 1983
Volume 21, Issue 3 - Sep 1983
Volume 21, Issue 2 - Jun 1983
Volume 21, Issue 1 - Mar 1983
Selecting the target year
Production Conditions and Properties of Glucose Isomerase from Streptomyces griseolus
The Korean Journal of Microbiology, volume 21, issue 2, 1983, Pages 51~60
Cultural characteristics of Strptomyces griseolus isolated from the soil were investigated. This strain was disclosed to utilize D-xylose, and D-glactose in preference order as a carbon source with the formation of glucose isomerase. The addition of sweet potato starch also proved effective promoting the total enzyme activity measured at 29% higher than the control. Corn cob, one of waste agricultural resources, was hydrolyzed in 2~3%
, 3~5 hours to produce a xylose syrup which gave rise to the recovery of 19.9% in a batch system and 28.2% in a repeated system. By the addition of both 2% of xylose syrup(Be'28) prepared by and us 65% of corn steep liquor (total nitrogen 1.2%), enzyme induction was maximized. The enzyme activity was stimulated by the xylose and the cell growth by the C.S.L. Also, remarkable increase of enzyme activity was noticed by the addition of protein acid hydrolysate 86.2% higher than the control.
of the biomass cultured in 30L capacity jarfermentor recorded low oxygen requirement of 251.2 1/hr. Maximum activity of glucose isomerase was observed noted at the 9th hour after inoculation which is 2 hours faster than the stationery was observed noted at the 9th hour after inoculation which is 2 hours faster than the stationery phase of the biomass growth. Glucose isomerase from the strain was activated by adding the
with optimum temperature of
and pH of 7.2. Conversion ratio of 60% glucose to frutose was 42.5% after 70 hours reaction.
Gene expression of feline leukemia virus(FeLV) in cat kidney cells with radioimmunoassay using beta-emission of
The Korean Journal of Microbiology, volume 21, issue 2, 1983, Pages 61~70
Synchronized cat kidney cells chronically infected with feline leukemia virus (FeLV) were used to study virus production, the synthesis of group specific antigen (gag) and envelope (env) proteins, the expression of env protein on the cell surface during the cell cycle, and the stability of viral RNA. As detecting method, we developed the radioimmunoassay (RIA) system using beta-emission of
and demonstrated the validity of this system by comparison with routine RIA system using gamma-emission of
. The produced virus was analysed by developed RIA interval was determined by measuring reverse transcriptase activity. The results show that infected cells produce the complete virus particle containing products of gag, env and pol genes of FeLV, and maximum virus production occurs during mitosis of synchronized cells. Labeling of the cell surface of synchronized cells with
shows that the amount of
on the cell surface parallels cellular gorwth. Therefore, the cell cycle-dependent release of virus is not petition RIA of synchronized cells with
labeled viral proteins synthesis during the cell cycle. The rate of synthesis of gag protein shows three peaks, corresponding to the
phases of cell cycle. But the rate of synthesis of env protein dose not change, suggesting that in these cells the synthesis of these two gene products in controlled seperately. In Actionomycin D treated cells, the synthesis of viral proteins decreased sharply from 8 hours after treatment, and the late S and
peaks of gag protein synthesis were disappeared. This shows the stability of viral RNA for about 6 hours in the absence of continuing viral RNA synthesis.
Studies on the fluctuation of aerobic free-living nitrogen fixation bacteria in soil beneath the plant covers
The Korean Journal of Microbiology, volume 21, issue 2, 1983, Pages 71~78
The number of aerobic free-living nitrogen fixation bacteria and factors in soil at different stands covered with Pinus rigida, Quercus acutissima and Zoysia japonica in Cheongju area were investigated from Feb. to Sept 1981. 1. The numbers of
bacteria, according to the seasonal changes, increased gradually from winter to spring and summer. But the growth pattern revealed some differences in accordance with plant cover stands : the numbers increased abruptly in May at Pinus, May-June at Quercus and Apr. May at Zoysia stand. The pick of numbers represented in Aug. Sept, at Pinus, Jul-Aug. at Quercus and May-Jun. at Zoysia stand, respectively. 2. The interrelationship between the monthly changes of enviotnmental factors and numbers of
bacteria at different stands, mainly depends upon the soil temperature than other soil factors (r=0.71-0.84). The numbers of
bacteria may increase 5-7 times according to increase
of soil temperature, and optimal range was
for growth. Equation of the interrelation between soil temperature and numbers could be stated as follows : log y=ax+b. 3. In the case of high soil temperature, the bacterial numbers presented high level in drought periods. Therefore, the
bacterial species in these soil seem to consist of resistant to desication. 4. The influence of soil organic matter for growth of
bacteria indicated low conrelation. The reason may seen the content of organic matter in these soil existed abundantly above the quantities of limitation for growth. 5. In artifical gradients, the
bacteria were predominated at
same as natural condition, pH7-8, and 20-30% of soil water contents. 6. The vertical distribution of bacteria marked decreasing trends from surface to lower layers, and the decreasing degree was shown well in Zoysia, Quercus and Pinus stand in order. But in the trees, the numbers increased at 30cm layer estimated the region of root than 20cm layer. 7. Both catalase megative and positive group of
bacteria in soil increased according to the rise of the soil temperature. Catalase positive group was revealed as dominant group in winter, and catalase negative group revealed in summer. The change of dominant pattern was shown during Feb. to Apr.
Ammonia oxidation activity of nitrifying bacteria and effects of some environmental factors
The Korean Journal of Microbiology, volume 21, issue 2, 1983, Pages 79~85
Ammonia oxidation activity of polluted water samples in Jinhae Bay and isolated strain from the seawater was investigated, and effects of environmental factors such as temperature, salinity, substrate concentration to the ammonia oxidation were also investigated. The ammonia oxidation activities of sediments, 0.01-0.04mg eq.
, were exceptionally higher than that of sea water,
. the activities of muddy sediments at station 4 and 2 were 0.03~0.04mg eq.
and that of sandy sediment at station 3 was 0.002mg eq.
. In the case of sea water, the activity of polluted area, station 1, was 2 times higher than that of offshore, station 4. The isolated strain reached log phase after 30days culturs and its oxidation activity was
. The maximum oxidation of ammonia by IA 13 strain occured at 30mg/l oxidation increased with the salinity rising up to 100% seawater concentraion. And temperature for maximum oxidation of ammonia was
. the oxidation increased with the salinity rising up to 100% seawater concentration.
Studies on the Pectolytic Enzymes from Byssochlamys fulva II
The Korean Journal of Microbiology, volume 21, issue 2, 1983, Pages 86~94
Polygalacturonase of Byssochlamys fulva was purified and characterized. Specific activity increased from 2.21 units/mg protein to 10.47 units/mg protein through
treatment, SephadexG-100 gel filtration, and DEAE-Sephadex ion exchange chromatography. Divalent cations, such as
, increased polygalacturonase activity greatly. Added as
ion enhanced enzyme activity 9.8folds. Optimum temperature was
and optimum pH was 5.0. Activation energy of reaction was 8.69 Kcal/mole. Michaelis-Menten
of reaction were
. Polygalacturonase of Byssochlamys fulva preferred polygalacturonic acid to pectin as substrate and was presumed as endo-type on the basis of the relationship between viscosity reduction and substrate degradation. Molecular weight of polygalacturonase was estimated as 55,000.
Studies on the structure and expression of penicillin G acylase gene I
The Korean Journal of Microbiology, volume 21, issue 2, 1983, Pages 95~102
The penicillin G acylase(pga) gene was cloned in the vector plasmid pKM
for the study of the structure and expression of the pga gene. This recombinant plasmid pPAKS-1 DNA(24.5 Kb) was cleaved into 2 fragments by restriction enzyme Eco R1.1fragment by BamH1, 4fragments by Hind III, and 2 fragments by Pst I. The pga gene was located on the Eco R1.Hind III-C fragement of pPAKS-1. The recombinant plasmids pPAKS-1 and pPAKS-2, in which the Hind III-B and Hind III-D fragments pPAKS-1 are deleted, are characterized. The results are summarized as follows : 1. Doubling times of bacterial strain bearing pPAKS-1 and pPAKS-2 are 90 and 60 minutes, respectively. 2. pPAKS-1 and pPAKS-2 are present at about 16-32 and 70 copies per cell, respectively, are 0.66 and 5.5 units, respectively, which represent 2-fold and 20-fold higher enzyme 4. pPAKS-1 is very unstable, but pPAKS-2 is stable.