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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 21, Issue 4 - Dec 1983
Volume 21, Issue 3 - Sep 1983
Volume 21, Issue 2 - Jun 1983
Volume 21, Issue 1 - Mar 1983
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A Study on Physiological conditions for hydrogen evolution by Rhodopseudomonass phaeroides K-7
The Korean Journal of Microbiology, volume 21, issue 3, 1983, Pages 109~114
Physiological conditions for hydrogen evolution by Rhodopseudomonas sphaeroides K-7 are examined. Larger amount of molecular hydrogen was evolved at
, pH6.8-7.0 under anaerobically illuminated condition of about 12, 000 lux by the organism. The heighest rates of hydrogen evolution were observed in the culture with the organic acids such as acetate, DL-lactate or DL-malate in media containing L-glutamate as a nitrogen source. Hydrogen was also evolved from glucose with the rate of
cells (dry weight). When glucose was adopted a a sole carbon source, however, conside erable time lag of about 20hours was required for hydrogen evolution. The resting cells stored at
under argon maintained the rate of hydrogen evolution in nearly about 90% of initial one even 40 days of storation.
The effect of some detergents on the changes of bacterial membrane
The Korean Journal of Microbiology, volume 21, issue 3, 1983, Pages 115~126
The results that the effect of 6 detergents on the structural changes and biochemical composition of bacterial membrane of Escherichia coli and Bacillus cereus are as follows ; 1. Population growth of the bacteria was increased in case of the treatment with palmitoyl carnitine and sodium deoxy cholate but was increased in case of the treatment with palmitoyl carnitine and sodium deoxy cholate but was decreased by sodium dodecyl sulfate and palmitoyl choline, in E.coli and was decreased by palmitoyl carnitine and palmitoyl choline at the low concentration, in B. cereus. 2. The electron micrograph showed that cell wall lysis or cell collapse were observed in the treatment of sodium dodecyl sulfate and palmitoyl choline, and also cell wall was condensed by triton X-100 and sodium deoxy cholate, in E.coli. And in B. cereus, endospore formation of the bacteria was stimulated by palmitoyl choline, and cell lysis or structural changes of the membrane were observed in the treatment of sodium dodecyl sulfate, sodium cholate, and triton X-100, respectively. 3. As to the effect of detergent on the biochemical composition of biomembrane, the content of carnitine, in E.coli, and B.cereus, the content of structural protein and phospholipid were decreased by treatment of sodium dodecyl sulfate and structural protein was denatured by palmitoyl choline. 4. The profile of membrane protein revealed that the bacterial membrane were composed of various proteins. By dint of this result, some of membrane proteins were solubilized or changed to small molecules by the treatment of sodium dodecyl sulfate and palmitoyl choline, in E.coli and membrane protein of the biomembrane by treatment of sodium dodecyl sulfate, sodium deoxy cholate, palmitoyl choline, and palmitoyl carnitine were confirmed to be different profile as compared with those of the control, in B. cereus. Therefore, it is suggested that sodium dfodecyl sulfate and palmitoyl choline soulbilized biomembranes or inhibited membrane transport and that palmitoyl carnitine and sodium deoxy cholate were used as an energy source or stimulating the membrane transport, in E.coli. And, it is suggested that all of detergents were inhibited biomembrane synthesis, expet saponin, in B.cereus.
Isolation and Indentification of Nematode-Trapping Fungi
The Korean Journal of Microbiology, volume 21, issue 3, 1983, Pages 127~134
42 strains belonging to the genus Arthrobotrys and 17 strains belonging to the genus Monacrosporium, were isolated from soil specimens collected from the various localities in South Korea. 4 species of Arthrobotry and 2 species of Monacrosporium, which are unrecorded in Korea, were identified according to the Harrd and Cooke classification key, respectively. The species identified are as follows ; A.conoides, A. oligospora, A. musiformis, A. oviformis, M. crystosporum nd M. salinum.
Purification and properties of polyphosphate phosphohydrolase from chlorella ellipsoidea
The Korean Journal of Microbiology, volume 21, issue 3, 1983, Pages 135~142
The presence of polyphosphate phosphohydrolase (PPPH) and tripolyphosphate phosphohydrolase (TPPH) in Chlorella ellipsoidea were confirmed from the cell-free extract of the algal cells and three forms of PPPH were isolated, purified, and measured Km-Vmax value and inhibitory effect by metal ions, respectively. PPPH was most active at pH7.2, whereas TPPH at pH 7.6. Both enzymes exhibited their maximum activity at
. For the manifestation of catalytic activity, divalent, divalent metal ions are needed, and the best activator for both enzymes was
. These enzymes were inhibited by
considerably. PPPH from Chlorella ellipsoidea was purified by ammonium sulfate fractionation, ion-exchange chromatography on DEAE-Sephadex A-25, and gel filtration on Sephadex G-100, and some properties of the three different fraction with PPPH activity
were found, i.e, PPPH has multiple form. The Km values of
and Vmax were 3.33 mM/min, 3.33 mM/min, and 2.67 mM/min, respectively. It was shown that the types of inhibition of
on the activities of three forms of PPPH were competitive inhibition.
PZ-peptidase activities in Streptococcus sanguis and other oral bacteria
The Korean Journal of Microbiology, volume 21, issue 3, 1983, Pages 143~148
The occurrence of PZ-peptidase in Streptococcus sanguis and other oral bacteria was investigated utilizing washed whole cells as the enzyme source and PZ-pentapeptide as its substrate. Under the culture conditions employed in the present study. Streptococcus sanguis strains, fresh isolates as well as laboratory strains, produced a broad range of the enzyme activity (0.5-7.9 unit/mg protein). The strains of both Streptococcus mutans and Lactobacilli showed low levels of activity (0-0.5 unit/mg protein for S. mutans). As compared with the enzyme activities of other bacteria, a moderate range of activity was produced by the strains of Strptococcus mitis nad Strptoccus salivarius. Actinomyces strains, like those of S. sanguis, produced a varying amount of activity (0-9.8 unit/ mg protein). A possible involvement of the oral bacterial PZ-peptidase in the metabolism of human saliva proteins is discussed.
Toxic effects of furfural on Pseudomonas fluorescens
The Korean Journal of Microbiology, volume 21, issue 3, 1983, Pages 149~155
Pseudomonas fluorescens, which had been known to be unable to degrade furfural, could utilize 0.03% of furfural as a sole carbon source in a culture with forced aeration. Lag period of this strain was lengthened by low concentration of furfural and growth yield reduced. High concentration of furfural over 0.1% showed killing effect on this strain. Cells of higher metabolic activity and of earlier growth stage were affected more seriously. The fact that even 0.05% of furfural showed no inhibition on respiration of this strain was confirmed with data on respiration rate in Warburg manometr. From these results, it was suggested that furfural show no inhibitory effect on external respination activities of P.fluorescens.
Formation, Regeneration, and Fusion of Protoplast of Micromonospora spp.
The Korean Journal of Microbiology, volume 21, issue 3, 1983, Pages 156~162
Conditions for effcient formation and regeneration of protoplasts of Micromonospora rosaria and Micromonospora purpurea were investigated. The state of inoculm, culture stage and growth in a medium containing partially growth-inhibiting concentration of glycing have significant effects on portoplasting. A high frequency of regeneration (up to 30%) was accomplished with a hypertonic regeneration agar medium defined by Okanishi for Strptomyces. Using the optimal conditions for protroplasting and regeneration, protoplast fusion of auxotrophic M.rosaria was carried out. Polyethylene glycol 1,000 was chosen for fusogenic agent. When signgle auxotrophs were used, the recombinant frequency of auxortrophic markers varied from 1.3 to 3.2%. Using two double auxotrophs, the recombinant frequencies of 0.7-4.3% were obtained. Much lower frequencies(three or more orders of magnitude) were observed by the conventional matings.
Effects of temperature on the biomass yield and the chemical composition of pseudomonas cells in continuous culture
The Korean Journal of Microbiology, volume 21, issue 3, 1983, Pages 163~169
Effects of temperature on the gorwth characteristics and the chemical composition of pseudomonas cells grown under glucose-or methanol-utilizing continuous culture were studied. In a glucose-utilizing continuous culture, optimum dilution rate, agitation, pH, and temperature, for the higher biomass yield were
, 7000rpm, pH 7.5, and
, respectively. But in a methanol-utilizing continuous culture, they were
, 600rpm, pH 8, and
, respectively. In methanol-utilizing continuous culture, the maximum production rate of the cells was 1.48g, dry wt./1/hr at a dilution rate of
, and the cell yield was 0.46g. dry wt./g. glucose. In the methanol-utilizaing continuous culture, the maximum production rate of the cells was 0.33 7g. dry wt./1/hr. at a dilution rate of
and the cell yield was 0.44g dry cell/g. methanol. The contents of protein of the cells increase with the increase ingrowing temperature (from 15 to
), more or less, while the contents of RNA nad carbohydrate of the cells decreased. However, DNA contents of cells growth under the various temperature ranges didn't change. As the temeprature of cultivation rises at a constant dilution rate, the efficiency of RNA in protein synthesis was increased, showing the decreases in the ratio of RNA to protein.
Studies on Epidemiology of Campylobacter fetus subsp. jejuni infection
The Korean Journal of Microbiology, volume 21, issue 3, 1983, Pages 170~176
Compylobacter fetus subsp. jejuni infection in Korea has been reported in 1981, but the epidemiological information was lacking at the report. Therefore this study was undertaken to determine the Campylobacter carrier rate of the healthy Korean and of the Korean chicknens, and the antimicrobial susceptibity of the isolates from chicken. 129 stool samples from man and 296 fecal samples from chicken were collected and screened to determine the rates of Campylobacter carrier. Forty-four chicken isolates from Seoul and 30 chicken isolates from Iri city were examined the antimicrobial susceptibility. 1. The carrier rate in were examined the antimicrobial susceptibility 2. The isolation rates of the Campylobacter from Iri chickens were 50.3%. The highest rate was 76.3% on February and July, while the lowest rate ws 20.7% on March. 3. All of chicken isolates from Seoul and Iri were susceptible to amikacin and chromphenicol. 63.3% of the Seoul samples showed resistant to erythromycin.