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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 21, Issue 4 - Dec 1983
Volume 21, Issue 3 - Sep 1983
Volume 21, Issue 2 - Jun 1983
Volume 21, Issue 1 - Mar 1983
Selecting the target year
Oxidation of Alkane Derivatives by Corynebacterium sp.
The Korean Journal of Microbiology, volume 21, issue 4, 1983, Pages 185~190
Twelve Microorganisms capable of utilizing diaminododecane were isolated from the soil by enrichment culture technique. Seven strains of these were identified as Corynebacterium. The isolated strains were tested for the ability to utilize as carbon source, 10 different kind of alkane derivatives containing CN,
, Cl, and SH groups. Laurylcyanide, dicyanooetane, chlorodecane, and dichlorodecane were not utilized by any of the isolated strains; putrescine dihydrochloride, cadaverine dihydrochloride, diaminododecane, and n-dodecane were utilized by all of the isolated strains; and all of the isolated strains except DAD 2-3 could utilize dodecylmercaptan. The alkane derivatives that did not serve as ,growth substrates were tested further in oxidation tests using resting cell preparation. Alkane derivatives that are being oxidized by all of the isolated strains are laurylcyanide and dichlorodecane. Dicyanooctane was also oxidized by all of the isolated strains except DAD 30L, chlorodecane was the only oxidized by the three isolated strains. The most remarkable substrate that is being oxidized is dichlorodecane containing CN groups diterminally. Evidence obtained with thin layer chromatography of ,ethyl acetate extracts of culture broth of isolated strains grown in some alkane derivatives shows that these alkane derivatives are degraded.
Studies on diaminododecane Utilization by Bacteria
Studies on Diaminododecane Utilization by Corynebacterium sp. DAD 2-3
The Korean Journal of Microbiology, volume 21, issue 4, 1983, Pages 191~196
A Corynebacterium sp. capable of utilizing diaminododecane (DAD) were isolated from the soil by enrichment culture. Among 9 different kinds of substituted alkanes containing CN,
, Cl, and SH groups (monoterminally or diterminally substituted) tested as carbon source, the isolate, designated as DAD 2-3, utilized DAD, putrescine dihydrochloride, dodecane and laurylamine. Dodecanethiol, thioanisole, decanedithiol, dicyanooctane, laurylcyanide,and dichlorodecane were not utilized. When emulgen 950 was added to the medium, the growth of DAD 2-3 was slightly accelerated. Isolate DAD 2-3 grown in the medium with DAD as carbon source formed .alpha.-ketoglutaric acid. Metabolic product of DAD 2-3 grown in a medium without nitrogen source was different from that of grown in a medium with
. When glucose, putrescine, n-dodecane and other alkane derivatives were tested in place of DAD, isolate DAD 2-3 yielded products different from those they formed with DAD suggesting specificity of DAD as a carbon source.
Biosynthesis of Nucleic Acid in Chloroplast Isolated from Chlorella Cells. I.
The Korean Journal of Microbiology, volume 21, issue 4, 1983, Pages 197~206
For the purpose of investigating the effect of nalidixic acid on the nucleic acid synthesis in chloroplast isolated from Chlorella ellipsoidea, cells were cultured in the media treated with nalidixic acid(20ppm) for 5 days. Aliquots cells were taken out at the inoculation and at intervals during the culture and growth rate of Chlorella cells measured. After extraction of nucleic acids in chloroplast isolated from these cells, their contents were analyzed by the base composition and the effect of nalidixic acid on the nucleic acid synthesis interpreted to compare with those of the control. 1. It was showed that the inhibitory concentration affected by nalidixic acid on the growth of Chlorella cells were 20ppm. 2. Because nalidixic acid had depressed the DNA replication in isolated chloroplast as well as whole cell system, these contents were markedly decreased in comparison with those of the control. 3. In the isolated chloroplast as well as in the whole cell system, nalidixic acid was decreased contents of base in the RNA by preventing RNA transcription.
The Degrdation of Pigment-Producing Furfural in Aquatic Waste
The Korean Journal of Microbiology, volume 21, issue 4, 1983, Pages 207~212
Isolated Gram-negative bacteria, being capable of degrading toxic, recalcitrant, and pigment-producing furfural, were tentatively identified as Pseudomonas testosteroni, Pseudomonas maltophilia, Klebsiella Pneumoniae, and Pseudomonas fluorescens. They exhibited synergistic effects between P. testosteroni and the others in the degradation of colourproducing furfural. Synergistic effects and possible sequence of its degradation were attempted by manometric technique. P. testosteroni could degrade furfural to decolourize it and produce ninhydrin-reaction postive substance (NPS) which could be utilized by P. maltophilia and K. pneumoniae and the latter two bacteria could ,degrade furfural to 2-furoic acid as an oxidized form. Finally 2-furoic acid was further oxidized by P. fluorescens. Once NPS and 2-furoic acid were produced, the degradation efficiency was enhanced by competing four bacteria against furfural and 2-furoic acid.
Isolation of protoplast from conidiospore of Trichoderma koningii
The Korean Journal of Microbiology, volume 21, issue 4, 1983, Pages 213~220
Conditions for isolation of protoplasts from conidiospores of Trichoderma koningii ATCC 26113 were tested. Maximum production of conidial protoplasts was obtained by preincubation of conidiospores on liquid minimal medium for 8 1/2 hrs. and by reaction with cell wall lytic enzyme for 3 hrs. Among effective cell wall lytic enzymes (Driselase, p-Glucuronidase, Novozyme and Zymolyase), Driselase was the most effective one on the production of conidial protoplasts. The production of conidial protoplasts was also enhanced by addition of 2-Deoxy-D-Glucose
into liquid minimal medium. Over 70% of the initial swollen conidia, preincubated in liquid minimal medium supplemented with 2-Deoxy-D-Glucose
, were converted to protoplasts by incubation with 2% (w/v) commercial lytic enzyme Driselase at
for 3 hrs. The reversion frequency of the conidial protoplasts was about 30 times (25-50%) higher than that of mycelial protoplasts (0.6-1.3%).
Rapid Purification of Glucose-6-Phosphate Dehydrogenase by Affinity Chromatography
The Korean Journal of Microbiology, volume 21, issue 4, 1983, Pages 221~228
An improved procedure for the rapid purification of glucose-6-phosphate dehydrogenase from extracts of Saccharomyces cerevisiae was developed by using affinity chromatography. Among six affinty media tested,
and Affi-gel Blue were more effective than others (i.e., Affi-gel Red, AMP-agarose, ATP-agarose, and
). Conditions to desorb the enzyme bound to the affinity media were examined to increase the purity as well as yield. The best result was obtained when the column was developed with a linear gradient of KCl (0-1.0M). In case of Affi-gel Blue, introduction of
(15mM) washing step prior to the salt gradient was most effective to remove
proteins. For a large scale preparation of G-6-P dehydrogenase higher recovery was obtained by Affi-gel Blue than
, however, the purity of the enzyme was decreased by 10 times if the former was used as the affinity medium. The capacity of Affi-gel Blue for G-6-P dehydrogenase was found to be 5 times higher than that of
. Furthermore Affi-gel Blue could be reused repeatedly and its preparation is relatively easier and less expensive than
Studies on the Organization and Transcription of Aspergillus nidulans tRNA Genes
The Korean Journal of Microbiology, volume 21, issue 4, 1983, Pages 229~237
Total tRNA genes from Aspergillus nidulans were cloned for the further investigation of the structure and expression of Aspergillus tRNA genes. Aspergillus DNA was isolated from spores and cloned into pBR322 plasmid DNA using BamHI and
ligase. The recombinant hybrid DNA was transformed into E. coli HB101 and some 30,000 transformants were initially selected. Of these, about 5,300 E. coli clones containing Aspergillus DNA inserted into plasmid pBR322 at BamHl site have been isolated. The hybridization data obtained from the labeled Aspergillus
indicated that 105 colonies carried the total tRNA genes. From the data above and cohybridization experiment, tRNA genes of Aspergillus nidulans seem to be twice more clustered than those of yeast.
Possible Use of Ti plasmid for Genetic Engineering of Higher Plants I. Isolation of Ti plasmid from Agrobacterium tumefaciens in Korea.
The Korean Journal of Microbiology, volume 21, issue 4, 1983, Pages 238~244
In order to obtain Ti plasmid which may be used in genetic engineering of higher plants, virulent Agrobacterium tumefaciens were isolated from soil with selective medium. For their identification, morphological and cultural characteristics were investigated in parallel with physiological and turnorigenesis test. 14 strains were isolated from various regions in Korea. 3 of them were identified as biotype 1 and the others were so heterogeneous that they are not yet conformed to any biotypes. However, Ti plasmid was isolated from A. tumefaciens KU 14 strain, one of the above mentioned 14 strains.