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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 22, Issue 4 - Dec 1984
Volume 22, Issue 3 - Jan 1984
Volume 22, Issue 2 - Jan 1984
Volume 22, Issue 1 - Jan 1984
Selecting the target year
Ecological Studies on Yeasts in the Waters of the Yeong San River Estuary
Chun, Soon-Bai ;
The Korean Journal of Microbiology, volume 22, issue 1, 1984, Pages 1~18
With 156 water samples collected from 39 locations in the Yeong San River estuary during the 12month period from March 1976 to February 1977, the seasonal distribution of yeast and the distributional pattern of yeast on salinity gradient have been investigated. An overall average number of yeast ranged from 52 to 487 viable cells (c.f.u.) per 100ml water sample. The highest count of yeast was obtained in spring while the lowest value came in summer. 933 yeast and one yeast-like fungus pertaining to 14 genera and 83 species were recovered, of which Candida were 29%, Debaryomyces 17.3%, Rhodotorula Glutinis were dominant forms in all locations as well as throughout the year. The population size of total aerobic bacteria, the amount of terrestrial imputs, and some of geographical and/or climatic factor appear to reflect the seasonal distribution of yeast as well as the composition of yeast species in an estuarine environ. Average number of yeast, species diversity, and particularly the number of fermentative and pseudomycelium-producing yeasts increased with decreasing salinity whereas nitrate-utilizing yeasts showed opposite trend, suggesting that salinity gradient can be used as a feasible detector for the distributional pattern of yeast in estuarine habitat.
Electron microscopic observations on the trapping of nematode by Arthrobotrys conoides
Park, Jin-Sook ; Park, Yong-Keun ;
The Korean Journal of Microbiology, volume 22, issue 1, 1984, Pages 19~28
The nematode-trapping process by Arthrobotrys conoides was investigated with the aid of scanning and transmission electron microscopy. 1. A. conoides captures nematode by means of three-dimensional network. 2. The wall of trap cell was thicker than that of vegetative hypha and the trap cell was more rich in cell organelles such as endoplasmic reticulum, mitochondria and electrondense granule. 3. The electron-dense granule, which could be found only in trap organs, gradually disappeared during its penetration into nematode cuticle. 4. The osmiophilic area was found at adhering site between the trap organ and nematode cuticle. 5. In some cases, any appressorium was not found at the site of penetration. 6. When the fungal-nematode culture was conserved for 2~3 weeks, numerous young nematodes were found to be adhered to spores, resulting in death.
Isolation and Identification of Pseudomonas Utilizing Hydrocarbon
Kim, Jeong-Kook ; Lee, Yung-Nok ;
The Korean Journal of Microbiology, volume 22, issue 1, 1984, Pages 29~34
238 strains of bacteria were isolated from sewage and soil samples collected mainly in Seoul and its suburbs by enrichment culture on crude oil or hydrocarbon minimal medium. Of the isolates, 68 strains were tentatively identified as the genus Pseudomonas, 11 strains as Alcaligenus, and 10 strains as Acinetobacter. Of the 68 strains of Pseudomonas sp., 35 strains were identified as P. aeruginosa, 5 strains as P. fluorescence, 10 strains as P. putida, and 2 strains as P. mendocina.
A System Development of the Protoplast Fusion of Streptomyces coelicolor
Kim, Jong-Su ; Lee, Se-Yong ;
The Korean Journal of Microbiology, volume 22, issue 1, 1984, Pages 35~40
Attempts were made to optimise protoplast formation and regeneration methods to improve the protoplast fusion frequencies of Streptomyces coelicolor. The yields of protoplast formation and regeneration were varied with different growth phase of the culture. Maximum yields were obtained when cells were taken from the late logarithmic phase. Protoplast formation reached almost its maximum with lysozyme treatment at a concentration of 2mg/ml without any other lytic enzyme. A high frequency of protoplast regeneration was accomplished by overlay method: the method gave 14% recovery of regenerated protoplast versus 1.8% recovery for monolay method. A recombinant frequency of 1.8X10^-2 was obtained by protoplast fusion using PEG 1000(50% w/v).
Isolation and Characterization of Bacterial Pathogens from Eels (Anguilla japonica) Cultured in Korea
Hah, Yung-Chil ; Hong, Soon-Woo ; Oh, Hee-Bok ; Fryer, John L. ; Rohovec, John S. ;
The Korean Journal of Microbiology, volume 22, issue 1, 1984, Pages 41~48
Twenty two cultures of pathogenic bacteria were isolated from cultured eels(Anguilla japonica) from Asan Hatchery. The bateria were characterized by their biochemical properties, serological relationships, infectivity to gold fish and susceptibility to various antimicrobial compounds. Fourteen of 22(64%) cultures were identified as Edwardsiella tarda, five (23%) as Aeromonas hydrophila and three (14%) as Vibro anguillarum. Edwardisiella tardo isolates proved to be the main cause of the disease in cultured eels. They were serologically homogeneous and their virulency to gold fish was higher than any of the other groups of bacteria tested. The virulence of 3 isolates were low in gold fish exposed to the bacteria by the waterborn route. Ten strains were tested for their susceptibility to 12 antimicrobial compounds and were resistant to from one to six drugs: in particular, tetracycline derivatives and sulfisoxazole.
Formation and Regeneration of protoplasts by Novozym 234 from Kluyveromyces fragilis N100 and Candida pseudotropicalis CBS607
Bai, Suk ; Chun, Soon-Bai ;
The Korean Journal of Microbiology, volume 22, issue 1, 1984, Pages 49~56
Formation and regeneration of protoplasts by Novozym 234 from Kluyveromyces fragilis N100 and Candida pseudotropicalis CBS607 were studied. This enzyme was more effective on cells grown at exponential phase than those at stationary one to convert intact cells into protoplasts. As osmotic stabilizer, ammonium sulphate was suitable for not only protoplast formation but also regeneration in K. fragilis as well as in C. pseudotropicalis. Optimal enzyme concentration was 3mg per ml in K. fragilis and 1~3mg per ml in C. pseudotropicalis, respectively. After the exposure of K. fragilis cells to 3mg per ml of enzyme for 3hr at 30
, approximately 95% of protoplast formation of all observed cells was obgained, while about 100% from C. pseudotropicalis under the same condition was produced. The regeneration frequency of protoplasts by this enzyme was much lower than that by snail enzyme(Glusulase) although Novozym 234 converted cells from above two species into protoplasts free of cell debris effectively, compared with Glusulase. Novozym 234 appears to be suitable for subcellular fractionation to obtain nuclei or other organelles rather than protoplast regeneration.
Studies on the developement of Stigmatella aurantiaca(I) -Effects of cations, pheromone, and GMP on the fruiting body formation-
Kim, Soo-Ok ; Kim, Young-Min ;
The Korean Journal of Microbiology, volume 22, issue 1, 1984, Pages 57~66
Cells of Stigmatella aurantiace developed in the light on the medium containing calcium, barium, or lithium ion formed fruiting bodies without stalk. Fruiting body with stalk was formed on the medium containing calcium ion and GMP (GMP-medium) even under the dark condition. On the medium containing calcium and pheromone (pheromone-medium), most cells were developed only into the stalk in the light and into the sporangium in the dark. The number of aggregate formed on the medium containing calcium ion (Ca-medium) was more than that formed on the medium containing calcium, potassium, and sodium ions (CPS-medium). The number of aggregate formed on the GMP or pheromone-medium was less than that formed on the Ca-medium. Both pheromone and GMP reduced the time required for aggregate formation when cells were developed in the dark. Light stimulated cells to form more aggregates in short time when it was introduced into the Ca-, CPS-, GMP-, or pheromone-medium.
Studies on the developement of Stigmatella aurantiaca(II) -Effects of pheromone and GMP on the protein synthesis during development-
Ku, Nam-On ; Kim, Young-Min ;
The Korean Journal of Microbiology, volume 22, issue 1, 1984, Pages 67~72
Effects of pheromone and GMP on the protein synthesis during development of Stigmatella aurantiaca under the starved condition were examined and compared with that of light on the same process. Light, pheromone, and GMP induced at least three, five, and two kinds of new proteins, respectively; one of them having molecular weight 30,0000 was the only protein present in all cases. It was also observed that there are an increase or decrease in the expression and disappearance or reappearance of several vegetative proteins during development under the each condition. It may be deduced from all these results that the mechanism of development in Stigmatella aurantiaca is a complex on which may be affected not only by a class of development-specific proteines but also by other classes of new proteins as well as several classes of proteins present in the vegetative cells.