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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 22, Issue 4 - Dec 1984
Volume 22, Issue 3 - Jan 1984
Volume 22, Issue 2 - Jan 1984
Volume 22, Issue 1 - Jan 1984
Selecting the target year
Isolation and Identification of the Genus Rhizobium
Ha, Nam-Ju ; An, Tae-Kun ; Lee, Yeong-Nok ;
The Korean Journal of Microbiology, volume 22, issue 2, 1984, Pages 73~76
From the root nodules of legumes distributed in South Korea, 74 strains of Rhizobium were isolated. The strains isolated were identified based on Bergey's Manual and Vincent's identification key. Following 5 species of Rhizobium were confirmed. R. leguminosarum, R. meliloti, R. phaseoli, R. trifolii, and R. japonicum
Studies On Induction of
-D-galactosidase In Candida kefyr
Chun, Soon-Bai ;
The Korean Journal of Microbiology, volume 22, issue 2, 1984, Pages 77~84
This examined some conditions for the induction of
-D-galactosidase synthesis in Candida kefyr CBS 834. The optimal pH, temperature, and inoculum size either for growth or
-D-galactosidase synthesis were 5.5,
and above 0.2 at A610nm, respectively. Enzyme activity began to increase at 2h after the addition of inducer, and continued to increase linearly up to
before reaching stationary phase, and thereafter its activity was decreased.
-D-galactosidase was induced either by lactose or galactose but not either by glucose or ethanol. The greater activity of
-D-galactosidase on galactose than on lactose indicated that the former might be natural inducer for
-D-galactosidase synthesis. The rate of its induction as a function of lactose concentration showed that enzyme activity increased linearly above 4mM, while it was very low below that. Glucose represed the induction of
-D-galactosidase, and the period of adaptation to inducer from other carbon sources was relatively short.
Isolation of Infectious Pancreatic Necrosis Virus from Goldfish (Carassius auratus) and Chum Salmon (Oncorhynchus keta) in Korea.
Hah, Youg-Chil ; Hong, Soon-Woo ; Kim, Mi-Hee ; Fryer, J.L. ; Winton, J.R. ;
The Korean Journal of Microbiology, volume 22, issue 2, 1984, Pages 85~90
Two viruses were isolated from kidney and spleen tissues of goldfish(Carasius auratus) and the ovarian fluid of chum salmon(Oncorhynchus keta). Both viruses replicated and produced cytopathic effect in EPC, CHSE-214, and CHH-1 cell lines at
. The isolates were resistant to pH 3 and choloroform. Antiserum to infections pancreatic necrosis virus(IPNV) serotype VR 299 neutralized the infectivity of both of the isolates. Electron microscopy showed that the particles had typical IPNV particle morphology with average diameters of 55nm, This paper describes the first isolation of viruses infecting cultured fish in Korea.
The changes in the activities of Cellulase and Xylanase of Aspergillus phoenicis during the life cycle
Oh, Chan-Seok ; Lee, Yung-Nok ;
The Korean Journal of Microbiology, volume 22, issue 2, 1984, Pages 91~96
The changes in the activities of Cellulase and Xylanase of A. phoencis during the life cycle were surveyed by using synchronized culture technique. Avicelase activity of the fungus was reached at peak in the initial hyphal growth stage, but decreased gradually during the conidiophore formation, vesicle-phialide formation, and sporulation stages in decreasing order. CMCase, Salicinase, and Xylanase activities were raised very high in the initial hyphal growth stage, but decreased gradually in conidiophore formation and vesicle-phialide formation stages, and then increased, more or less, in the sporulation stage.
Degradation Pattern of CMC, Xylan, Lignin Components of Rice Straw by Bacillus subtilis DO4
Choe, Yeong-Tae ; Kim, Kyu-Jung ;
The Korean Journal of Microbiology, volume 22, issue 2, 1984, Pages 97~101
To investigate the biodegradation pattern of rice straw, mainly composed of cellulose, hemicellulose and lignin components, by the isolate stran Bacillus subtilis
, the change of cell population was observed on CMC (carboxymethyl cellulose), larch wood xylan and lignosulfonate as a carbon source respectively. Also, the transition pattern of enzyme activities of cellulase and xylanase and lignin contents was measured on rice straw and mixed substrate according to growth. The results in these experiments revealed that xylanase activity was first appeared and cellulase activity in the next, while lignin component was almost not changed through the culture period.
Intraspecific Protoplast Fusion in Trichoderma koningii
Hong, Soon-Woo ; Hah, Yung-Chil ; Park, Hee-Moon ; Cho, Nam-Jin ;
The Korean Journal of Microbiology, volume 22, issue 2, 1984, Pages 103~110
The conditions for the protoplast fusion of auxotrophic mutants of Trichoderma koningii were determined. A preparation of commercial enzyme Driselase was used successfully to isolate protoplasts from the 18 hr old mycelium of T. koningii. The yields of protoplasts production were ranged from
protoplasts per mg of damp mycelium of various auxotrophic mutant strains. The regeneration frequencies from
were obtained when the protoplasts from auxotrophic mutants were plated on the malt extract medium containing 0.6M
, and 2% agar, and the optimal concentration of PEG for protoplst fusion was 30%. Exposure of protoplasts to PEG for 10 min was found to be sufficient to induce high frequency heterokaryon formation. Optimal pH of fusion mixture was determined as 5.5, and 1 mM of calcium chloride in fusion mixture was found to be sufficient to enhance protoplast fusion frequency. Under optimal condition, the fusion frequency of the cross between protoplasts from various auxotrophic mutants were
Susceptibility of Bacillus subtilis SNU816 to bacteriophage SP816 during growth and sporulation.
Lee, Oh-hyoung ; Lee, Zoo-Shik ;
The Korean Journal of Microbiology, volume 22, issue 2, 1984, Pages 111~118
The changes of susceptibility of Bacillus subtilis SNU816 to bacteriophage SP816 were investigated. When B. sutilis SNU816 cells were infected by the phage during vegetative growth, rapid lysis was observed. But when they were infected after late logarismic phase, they were resistant to phage infection. Since asporogenic culture of this strain was invariably lysed regardless of time of infection, the arrest of phage multiplication seemed to be caused by sporulation. In reality, the arrest of phage multiplication occurred at early stage of sporulation. Electron microscopy revealed that the arrest of phage multiplication occurred just prior to or during septum formation (stage II sporulation).