Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 22, Issue 4 - Dec 1984
Volume 22, Issue 3 - Jan 1984
Volume 22, Issue 2 - Jan 1984
Volume 22, Issue 1 - Jan 1984
Selecting the target year
Biotin Requirement for the Growth and Sporulation of Bacillus subtilis SNU816 in a Synthetic medium
Lee, Oh-Hyoung ;
The Korean Journal of Microbiology, volume 22, issue 3, 1984, Pages 135~142
The effect of biotin on the growth and sporulation of Bacillus subtilis SNU816 was investigated. When B. subtilis SNU816 was cultured on glucose as a sole carbon source, the growth was retarded markedly and usually ceased at early log phawe. But by addition of biotin to this medium, normal, rapid growth was restored. The growth rate was increased proportionally according to the concentration of exogenous biotin until it reached to 0.05㎍/ml, at which about three fold rapid growth was achieved. Also biotin was required for optimum sporulation for it facilitated the complete utilization of both glucose(Glc) and glutamic acid(Glu). Without biotin in Glc+Glu medium, about 40% of glutamic acid was remained unutilized. The dipicolinic acid content of cells cultured in Glc+Glu medium without biotin was markedly small and sporulation was suppressed before free spore release. Since biotin could be partiallyreplaced by one of TCA cycle intermediates such as oxalacetic acid, citric acid, or glutamic acid in enhancing growth in Glc medium, it was postulated that this strain might have a defect in converting pyruvate to oxalacetate which process is known to be mediated by pyruvate carboxylase that requires biotin as a cofactor.
Induction of Colonial Growth of the Cellulolytic Fungus, Genus Trichoderma
Park, Hee-Moon ; Hong, Soon-Woo ; Hah, Yung-Chil ;
The Korean Journal of Microbiology, volume 22, issue 3, 1984, Pages 143~150
The effect of growth inhibitors on colonial growth of cellulolytic fungus, genus Trichoderma, was investigated to develop a method for the effective screening of various auxotrophs and hypercellulolytic strains. As the results, it was revealed that non-ionic detergents such as Sodium deoxycholate and Triton X-100 were the better ones as a growth inhibitor than Oxgall which has been used to restrict the colony size in genus Trichoderma. Each individual colony remained distinct on minimal plate supplemented with 0.05% Triton X-100 for as long as two or more weeks. The screening of 150 to 200 colonies simultaneous on a single plate was possible in the presence of Triton X-100. The effect of Triton X-100 on simultaneous screening was higher than that of Oxgall by a factor of two.
The effect of Palmitoylcarnitine and Ginseng Saponin on the nutrient uptake in Escherichia coli B.
Kim, Soon-Ok ; Lee, Ho-Yong ; Lee, Chong-Sam ; Choi, Yong-Keel ; Cho, Key-Seung ;
The Korean Journal of Microbiology, volume 22, issue 3, 1984, Pages 151~156
When enterobacterium, Escherichia coli B was cultivated with normal media in the presence of
Ginseng Saponin, maximum population growth of the bacteria was presented 71% and 31%, respectively. Such a result, in vitro test, was concluded from the result that both detergents stimulated
-phosphatidylethanolamine uptake into the membrane of cells. The pre-treatment of cells with different amounts of Palmitoylcarnitine from
moles represented a significant increase of uptake, 33% of
-glucose, 129% of
-alanine and 158% of phosphatidylethanolamine at the concentration of
moles of Palmitoylcarnitine. On the other hand, the result of
Saponin treatment showed the maximum value of uptake, 17% of
-glucose and 112% of
-alanine. In case of
-phosphatidylethanolamine, the maximum uptake showed 25% of increase at the concentration of
Seasonal and Spatial Distribution of Trichoderma species in Forest Soils of Mt. Geryongsan
Rhee, Young-Ha ; Hong, Soon-Woo ;
The Korean Journal of Microbiology, volume 22, issue 3, 1984, Pages 157~165
Seasonal and spatial variations in propagule numbers of Trichoderma species were investigated every other month for one year in deciduous and coniferous forest soils and evaluated the relationships of Trichoderma spp. populations to soil environmental factors. The total population of Trichoderma spp. increased until summer and then declined until winter. The yearly mean frequency of Trichoderma spp. exceeded 1.4% of total fungal propagules in two sites. Decreases of absolute an relative propagule numbers of Trichoderma spp. with increasing soil depth were found and variation in Trichoderma spp. propagules caused by differences in soil depth (
) was greater than that caused by differences in sampling time. The most common species occurring in two sites was T. viride, followed by T. polysporum, T. koningii, and T. hamatum. Individual species of Trichoderma showed diferent abundance trend in accordance with sampling time. T. viride was dorminant from spring to autumn, while T. polysporum dominated over the other speicies in winter. Variations in propagule number of Trichoderma sppp. were principally mediated by the actions of biotic environmental factors rather than by the direct effects of abiotic factors. In multiple-regression analyses, 48% of the total vaiation in Trichoderma spp. propagules in deciduous site could be accounted for by total fungal propagules and soil CMCase actvity. In coniferous site, 65% of total variation could be accounted for by total fungal and bacterial propagules, moisture content and organic carbon content.
Studies on Molecular Improvement of Cellulose Utilizing Bacterial Strain -Molecular cloning of
-glucosidase gene of Cellulomonas sp. in E. coli-
Bae, Moo ; Lee, Jae-Moon ;
The Korean Journal of Microbiology, volume 22, issue 3, 1984, Pages 167~173
The cellabiase (
-glucosidase) gene in a Cellulomonas sp. CS1-1 was cloned into E. coli HB101 using the vector plasmid pBR322, and the expression of the gene in E. coli studied. The chromosomal DNA of the cellulomonas was digested by seveal restriction enzymes, each of which has only one cleaving site in plasmid pBR322. The recombinant plasmid, pSB2, created with Sal I frament, was expressed for the cellobiase gene in E. coli. The recombiant plasmid was estimated to contain 6.4 Kb foreign DNA at the Sal I site of plasmid pBR322 and the inserted DNA was mapped by single and double digestion with several enzymes. E. coli HB101(pSB2) has slowly grown in a mineral liquid medium containing cellobiose as a sole carbon source. The cellobiase activity in the transformed E. coli was 132 units per liter, which is equivalent to one twenty fifth of that in doner strain Cellulomonas sp. CS1-1. The transforned cell with plasmid containing cellulase gene grow well in the LB mediuns. The synthesis of cellobiase in the strain, E. coli HB101 (pSB2), was inhibited by glucose and at high concentration of cellobiose, and induced by cellobiose at low concentration.
The protoplast formation, regeneration and fusion of coryneform bacteria
Shin, Myung-Gyo ; Lee, Se-Yong ; Lim, Bun-Sam ; Chun, Moon-Jin ;
The Korean Journal of Microbiology, volume 22, issue 3, 1984, Pages 175~181
In order to develope a protoplast fusion system for industrial coryneform bacteria, the optimum conditions for the formation and regeneration of progoplast were examined for Brevibacterium flavum and Corynebacterium glutamicum and the protoplast fusion was performed. For the formation of the protoplast of B. flavum and C. glutamicum, the optimum time for penicillin G. treatment to obtain protoplast was mid-exponential growth phase (
). At the optimum conditions (0.3units/ml penicillin G and
lysoyme for treatement), frequencies of protoplast formation and protoplast regeneration were 99% and 25%, respectively. Protoplast regeneration frequency was highest under the optimum conditions for the protoplast formation. Addition of 25mM
to the regeneration medium further increased the regeneration frequencies. The protoplast fusion frequencies of B. flavum and C. glutamicum in intraspecies fusion were
, of the regenerated protoplast respectively, when 33% of PEG (polythylene glycol) 6,000 was used as the fusing agent.
Identification of Responsible Region for the Polymerization of Plasmid pEC-3
Jang, Sung-Key ; Lee, Ha-Kyu ; Rho, Hyune-Mo ;
The Korean Journal of Microbiology, volume 22, issue 3, 1984, Pages 183~189
In order to find specific acting site of Rec A protein in plasmic polymerization in E. coli, we randomly deleted various part of pEC-3 (a derivative of pBR322) with SI nuclease treatment. Self-ligated plasmids were introduced into E. coli WA802(Rec
). A number of colonies were analyzed if they contained monomeric or polymeric plasmids by gel electrophoresis. The plasmid (pEC-43), which was deleted the region of tetracycline gene, revealed only monomeric form in Rec
E. coli. When two plasmids, pEC-3 and pEC-43, were co-transformed in the same E. coli, the original pEC-3 showed polymerization but pEC-43 revealed monomeric form only. These results suggest that Rec A protein requires the specific site for polymerization.
Studies on the Microbial Pigment (V) The effect of some detergent on pigment formation in Serratia marcescens strain P
Lee, Ho-Yong ; Cho, Hong-Bum ; Choi, Yong-Keel ;
The Korean Journal of Microbiology, volume 22, issue 3, 1984, Pages 191~195
In order to study on the pigment formation of Serratia marcescens, the synthesis of prodigiosin was examined in the presence of a wide range of concentration of detergents. A high elevation of pigment formation was obtained in case of the treatment with SDC and SAP. And the population growth of the bacteria was increased by SDC and SAP, in the concentration of optimum concentration of pigment formation. The alkaline phosphatase activity was also increased in the treatment of SAP, SDC and SDS. The possible mechanism of the detergents on enhancement of pigment formation could be explained by an increase of enzyme activity and membrane transport.
Chromosomal studies on the genus of Rhizopus -II. Chromosomal studies on 17 species of the genus Rhizopus-
Min, Byung-Re ;
The Korean Journal of Microbiology, volume 22, issue 3, 1984, Pages 197~205
After the previous paper, this chromosomal studies on the fungi were dealt with 17 species in genus of Rhizopus. The results are sumarized as the followings; The haploid chromosome number of 17 species were confirmed as of 6(Rh. oligosporus), 8(Rh. homothallicus, Rh. liquefaciens, Rh. shanghaiensis, Rh, acetorinus), 12(Rh. microsporus, Rh. pseudochinensis, Rh, rhizopodiformis, Rh, thermosus, and Rh. kazanensis), 14(Rh. stolonifer), and 16(Rh. suinus), respectively. Referring to the above fact and the previous paper, it is strongly presumed that the basic chromosome number of Rhizopus are 4.