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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 23, Issue 4 - Dec 1985
Volume 23, Issue 3 - Sep 1985
Volume 23, Issue 2 - Jun 1985
Volume 23, Issue 1 - Mar 1985
Selecting the target year
Acinetobacter Isolates Growing with Carbon Monoxide
The Korean Journal of Microbiology, volume 23, issue 1, 1985, Pages 1~8
Three strains (JC1, JC2, and HY1) of aerobic carbon monoxide (CO)-utilizing Acinetobacter were isolated from soil through CO-enrichment culture technique. All of them were Gram-negative, nonmotile, and rod-shated but they were changed to spherical form at the end of logarithmic phase. They were resistant to penicillin and able to frow at
. The guanine plus cytosine contents of the DNAs ranged from 43 to 44.5 mol%. Oxidase was not present in all cells. The colonies were smooth and whitish yellow. Heterotrophic growth occurred on several sugars, organic acids, amino acids, and alcohols. The doubling times under and atmosphere of 30% CO and 70% air at
were 19h, 25h, and 35h, respectively, for JC1, JC2, and HY1, JC1 was studied in more detail. The cells were grown optimally in a mineral medium (pH 6.8) under a gas mixture of 30% CO and 70% air at
. Growth of the cells with CO did not depend on molybdenum. It was able to grow with 100 ppm of CO in air as a sole source of carbon and energy.
mutation on the tryptophan excretion in escherichia coli
The Korean Journal of Microbiology, volume 23, issue 1, 1985, Pages 9~12
As a part of the host cell development for a amplified recombinant trp operon,
mutation was introduced in a E. coli host strain.
mutation was induced by transposon Tn10 and transduced into the E. coli host cell by bacteriophage P1Kc. The effect of
mutation on the excretion of tryptophan in E. coli
cells was investigated. Mutant lacking the general aromatic transport system was resistant to
, or 5-methyltryptophan
uptake of the
mutant strain was reduced considerably as compared with
counterpart. The rate of
uptake of the
mutant strain treated with
was much less affected than that of
transductants increased the tryptophan excretion from E. coli
four times more than
Purification and Characterization of Acc I Endonuclease
The Korean Journal of Microbiology, volume 23, issue 1, 1985, Pages 13~19
Acc I endonuclease has been isolated from 300g (wet weight) cells of Acinetobacter calcoaceticus. The cells were broken by using French press at 20, 000p.s.i. After ammonium sulfate fractionation, the enzyme was further purified by heparin agarose, DEAE-sephades, Affi.-gel Blue, phosphocellulose, and hydroxylapatite column chromatography. The purified Acc I endonudlease has a single polypeptide species and its subunit molecular weight was 45,000
1,000 daltons as judged by 10% SDS-polyacrylamide gel electrophoresis. The isolated enzyme was essentially free of contaminating nucleases as judged by homochromatography by using a
oligonucleotide. The enzyme showed maximum activity at pH values between 8.0 and 11.0 and in the presence of
. Acc I endonuclease was maximally active in the absence of NaCl and was completely inhibited at 200 mM NaCl.
The Effects of Nitrogen Sources on the Expression of Nif Gene in Klebsiella pneumoniae Nif-Lac Fusants
The Korean Journal of Microbiology, volume 23, issue 1, 1985, Pages 20~24
The effects of various nitrogen soruces on the expression of nif gene were investigated using nif-lac fusants of Klebsiella pneumoniae. K. pneumoniae UK 2979 was infected with Mudl lysate prepared by heat induction of K. pneumoniae UK 4482. About 80 nif-lac fusants were isolated and designated as LX series. In the prescence of
activities on nif-lac fusants were greatly repressed. Amino acids, such as serine, glutamine and asparagine, were found to support the growth of K. pneumoniae M5al quite well, and showed a repressive effect on
activities of nif-lac fusants LX-9 and LX-22 in NFHM. Glutamic acid, histidine and arginine rendered poor growth but high activities of
. Good cell growth and high enzyme activity were observed when complex nitrogen sources, such as casitone, proteose pepone, were employed.
activities of LX-9 and LX-22 in nitrogen free minimal medium increased sharply within first 4 hours.
Fractionation of Extracellular Cellulase Pproduced by Cellulomonas and Reaction Mechanisms of the Isolated Enzymes
Kim Byung Hong ; Wimpenny, J.W.T. ;
The Korean Journal of Microbiology, volume 23, issue 1, 1985, Pages 25~33
The cell-free cellulolytic enzyme was separated into 3 different enzyme proteins by gel-filtration and ion-exchange chromatography. These fractions were named enzyme A, enzyme B and enzyme C. The mode of action of each of the separated enzymes on crystalline cellulose was examined using infrared spectroscopy and X-ray crystallography. It was concluded that enzyme B is of the
and reduces the crystallinity of the subatrate by generation an unstable glucopyranose ring structure, whilst enzymes A and C are of the
and hydrolyse the reaction product of enzyme B to constituent sugars. A reaction scheme for this cellulase system is proposed and discussed.
Characterzation of Cellulose Nonutilizing Mutants from Aspergillus nidulans
The Korean Journal of Microbiology, volume 23, issue 1, 1985, Pages 34~42
From Aspergillus nidulans FGSC 168, mutant strains (TCD27, NCN14, and ACN3) were obtained by NTG and 4-NQO treatments. These four mutant strains were unable to grow on medium supplemented with CMC as a sole carbon source and showed lower levels of CMCase activity in comparison with FGSC168. Some characteristics of these four mutant strains in CMCase production were examined. It was appeared that the permeases for CMCase inducer in all the four mutants were not affected. Two mutants, TCD27 and NCN2, might have some defects in regulatory gene for CMCase production. Analyses of enzyme components of CMCase showed that ACN14 might by defective in structural gene for CMCase.
Isolation of Anaerobic Bacteria from Oral Pyogenic Infections
The Korean Journal of Microbiology, volume 23, issue 1, 1985, Pages 43~48
Strict anaerobic procedures and anaerobic chamber were employed in order to improve the isolation of obligate anaerobes from oral pyogenic infections. Also different culture media were utilized to maximize bacterial recovery. Two blood media: nalidixic acid Tween blood agar (NATB) and plain blood agar (BA), two non-blood media: nalidixic acid Tween agar (NAT) and Gifu anaerobic medium (GAM) were used and compared for their isolation efficacy. Specimens from seven patients were collected with syringe. After collection, the needle was sealed with sterilixed silicone rubber and brought to labortory. The time spent from specimen collection to its processing in anaerobic chamber usually was 15 min. Identification of isolated bacterial strains was done with the API-20A system. Increase in isolation of anaerobic vacteria was achieved. Obligate anaerobic bacteria isolated were 3.3 strains per specimen. This figure falls within the range of 1.9-4.4 strains per specimen reported in other countries. With respect to the media utilized, blood media were superior to non-blood media. NATB medium was effective especially for the isolation of Gram-positive cocci. A total of 15 species of Gram-negative rods was isolated and 12 of them belonged to Bacteroides.
Comparison of laboratory and in situ studies on the survival of escherichia coli in river waters
The Korean Journal of Microbiology, volume 23, issue 1, 1985, Pages 49~55
The survival of Escherichia coli in river water was comparatively studied by laboratory and in sity study methods. The survival by two methods was evaluated as a function of E. coli strain, indigeneous predator, level of water pollution, and water temperature in different season. The survival rate of E. coli examined by laboratory method was lower than that by in situ method. That was found to be due to the fact that higher number of predator was maintained in labortory study than in in situ study. The survival rates of E. coli in gradually polluted river waters could be differentiated by in situ study, but not by laboratory study. Therefore, an in situ method rather than labortory method was thought to be a choice of study method for the survival of E. coli in river waters.
Characterization of plasmids of Zymomonas mobils and Construction of E. coli-Zymomonas shuttle Vector
The Korean Journal of Microbiology, volume 23, issue 1, 1985, Pages 56~63
We have characterized the plasmids of zymomonas, and constructed E. coli-Zymomonas shuttle vector. Plasmids have been detected in four strains of Zymomonas mobilis. All strains tested had at least one plasmid ranging in size from about 1.7 to 46kb. Antibiotics resistances of Z. mobilis were tested to select the host strain. All strains were very sensitive to tetracycline and chloramphenicol. Homology tests between the plasmids in four strains showed that the plasmids of ATCC10988 is highly homologous to those of ZM1, and that there is no homology between plasmids of ZM4 and Agll. The 1.7kb plasmid of ATCC10988, named as pZM886, also has no homology with plasmids of ZM4. A hybrid plasmid, designated to pBZ41, was constructed from pZM886 and pBR322. A restriction map of pBZ41 was established. Replicon of pZM886 didn't operate in E.coli and pBR322 seemed not to replicate in Zymomonas. pBZ41 was transfered from E. coli to Zymomonas by conjugal mobilization. The transconjugants were resistant to tetracycline and maintained pBZ41 stably.
Characterization of Mouse Hybridoma Producing Monoclonal Antibody Paragonimus westermani
The Korean Journal of Microbiology, volume 23, issue 1, 1985, Pages 64~68
In this study, hybridoma techniques were applied to produce monoclonal antobodies to Paragonimus westermani, commonly known as lung distoma. Balb/c mice were immunized intraperitoneally every week with increasing doses of purfied protein of Paragonimus westermani adult worms begining with 0.3/mg/mouse/7 days and ending with 0.5mg at 28th day. Spleen cells from these immunized mice were hybridized with myeloma cells (NS-1) and the hybridized cells were selected in HAT media. The antibody secreting cells among the hybrid cells were initially selected by ELISA. Those initially selected cells were further screened by the criteria of antibody producing activity, and seneral cell lines among them were further tested with immunodiffusion method. One hybridoma clone produced IgM and another clone produced IgG1. The supernatant of the hybridoma clone producing IgM had titer 1:64 and the hybridoma clone producing IgG1 had titer 1:256 measured by immunofluorescence technique.
A Study on Superoxide Dismutase from various Tissue of the Tricarboxylic acid cycle blocked Rat
The Korean Journal of Microbiology, volume 23, issue 1, 1985, Pages 69~76
This study was carried out to observe the formation of superoxide radicals and the changes in the activities of superoxide dismutase (EC.18.104.22.168.) from the various organs of a rat which was blocked tricarboxylic acid cycle. In order to block the tricarboxylic acid cycle, the beta-fluoroethylacetate was injected into peritoneal cavity of rat and removed the various tissues from the rat at internals of an hour. By tissue extracts being prepared by the method of Weigiger and Fridovich the activities of superoxide dismutase, aconitase, and contents of bliid glucose, citrates, and wuperoxide radicals were determined. The experimental results are summarized as follows: Accumulation of citrates if increased within three hours after treatment in the all tested tissues, especially, in the geart and spleen they are higher than one of other tissues as 12 and 20 times of control. The activities of aconitase are ingibited to 30-35% on an hour after beta-fluoroethylacetate treatment comparing with that of control rat. The content of blood glucose is increased to 1.6 fold of normal value after 5 hours of treatment. In all tested tissues, superoxide radicals are formed in the heart as 0.26 micromoles per gram tissue between one and three hours after treatment. The activities of total superoxide dismutase are increased between one and three hours after treatment in the all tested tissues and one of these enzymes in heart is highest. The activities of superoxide dismutase containing Mn are also increased with an increase of total superoxide dismutase activities.