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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 23, Issue 4 - Dec 1985
Volume 23, Issue 3 - Sep 1985
Volume 23, Issue 2 - Jun 1985
Volume 23, Issue 1 - Mar 1985
Selecting the target year
Aspects of Cellulase Induction by Sophorose in Trichoderma reesei QM9414
The Korean Journal of Microbiology, volume 23, issue 2, 1985, Pages 77~83
The aim of this investigation was to resolve the contradiction between the results of Sternberg and Mandels (1980, 1982)and those of Nisizawa et al., (1971) in cellulase induction by sophorose, and furthermore to study the conditional effects in sophorose-induced cellulase induction in Trichoderma reesei QM 9414. Sophorose could induce the synthesis of CMCase and
simultaneously. Optimal induction medium by sophorose had the potassium citrate buffer solution of pH 3.0-4.0 for CMCase, but one of pH 5.0-6.0 for
. At this time, two different types of
could be induced by sophorose: one was extracellular and had maximum at pH 5.0, the other was intracellular and had maximum activity at pH6.5. Induction study showed that
was not a true inducer of
and that large
induction could be obtained only by the addition of sophorose into the induction medium. Glucose repressed the induction of cellulase by sophorose. The repression of glucose could not be overcome by the addition of cyclic AMP into the induction medium.
Studies on the Changes in Activities of ALPase, ACPase, ATPase and Synthesis of Volutin Granules upon Phosphate Concentration in Saccharomyces uvarum
The Korean Journal of Microbiology, volume 23, issue 2, 1985, Pages 84~89
The effect of exogenous phosphate supply on the regulation of phosphate metabolism was investegated during catabolic repression and catabolic derepression in yeast (Saccharomyces uvarum). As the results, when sugar was supplimented in cells cultivated under phosphate free, the growith rate was low but it was capable of cell division. Polyphosphate "B" was accumulated highly in proportion to amount of phosphate added to the medium. Without regard to phosphate supply of the medium, the total amount of polyphospgate was almost similar, although each polyphosphate was turned over. Activities of all phosphatases remained continuousoy high in the cells cultivated in the phosphate free medium. Especially under catabolic repression, the function of polyphosphate system was shown to compensate the ATP/ADP system as phosphate donor, energy source and regulator.
Studies on the activities of ALPase, ACPase, ATPase and accumulation of volutin granules upon growth phase in saccharomyces uvarum
The Korean Journal of Microbiology, volume 23, issue 2, 1985, Pages 90~100
The present study was designed to investigate cellular regulation of phosphate metabolism between catabolically repressed and derepressed states in yeast (Saccharomyces uvarum). The activities of various phospatases and the contents of phosphate compounds were detected according to the culture phase and various phosphate concentrations. As the results, Saccharomyces uvarum derepressed many phosphate metabolizing enzymes such as alkaline phosphatase, acid phosphatase and ATPase more than ten fold simultaneously during catabolic repression (phospgate and sugar starvation). At the same state, the amounts of orthophosphate, nucleotidic labile phosphate and acid soluble polypgosphate were increased, compared to basal levels of normally cultivated cells.
type among all phospatases was appeared to have most of the enzyme activity. It could be postulated that
alkaline phosphatase was directly or indirectly correlated with the synthesis of acid insoluble polyphosphate
phosphatase with the degradation of polyphosphates. In case of cultivation in the medium supplemented with sugar and phosphate (catabolic derepression), phospgatase activities except for alkaline phosphatase were decreased rapidly through the progressive batch culture, After 12 hrs culture, at early exponential phase, the cellular accumulation of acid insoluble polyphosphate increased about 5 fold, compared to those of the starved cells. Under catabolic repression, it could be postulated that intracellular phosphate metabolism was regulated by derepressions of phosphatases. The function of polyphosphate system was shown to compensate the ATP/ADP system as phosphate donor and energy source especially during catabolic repression.
Effect of Ethanol Concentration on the Rates of Cell Growth and Ethanol Production in Zymomonas mobilis
The Korean Journal of Microbiology, volume 23, issue 2, 1985, Pages 101~106
The effects of ethanol on the specific rates of growth and ethanol production were found to be threshold and linear inhibition. The degree of inhibition was more apparent on the specific growth rate while ethanol production was continued even the growth was ceased. The nature of uncoupling between the growth (anabolism) and ethanol production (catabolism) was clearly observed under high concentration of ethanol. The uncoupling indicated that ethanol concentration plays a great role in maintenance energy coefficient.
Transfer of RP4:Mu cts from E. coli to Rhizovium leguminosarum
The Korean Journal of Microbiology, volume 23, issue 2, 1985, Pages 107~114
In order to use for recipient strains of RP4:Mu cts, 5 strainsof Rhizobium were selected among 32 strains, which were isolated and identified in this study. Hybrid plasmin RP4::Mu cts, which, is temperature sensitive and confers resistance to ampicillin, kanamycin and tetracycline was transfered by conjugation from E. coli to other atrains of C. coli and the symbiotic nitrogen fixer, Rhizobium leguminosarum. Transfer frequencies of RP4::Mu cts plasmid from E. coli to Rhizobium were about
in LB agar and YMA media. The transconjugants were confirmed by demonstrating that the drug-resistant and temperature-sensitive clones isolated were drug-resistant and temperature-sensitive clones isolated were capable of releasing phage and forming plaques. The plaque-forming units of transconjugants were about
. Stability test of RP4::Mucts in Rhizobium represented that most of the transconjugants had drug resistance and produce phage Mu cts.
Classification and Characterization of Bacteriophages of Lactobacillus casei -Analysis of Restriction Patterns of Phage DNA-
The Korean Journal of Microbiology, volume 23, issue 2, 1985, Pages 115~121
Five representative virulent phages (J1, TK93, K1, PD5, and CP1) and one temperate phage (.phi.1043) of Lactobacillus casei were compared to each other by analyzing the agarose gel electrophoretic patterns of restriction enzyme-digested phage DNAs. Nucleic acids of all the tested phages were double stranded DNA. DNAs of J1, TK93, K1, and
1043 phages had a size of about 42kb, but the size of PD5 and CP1 DNAs was avout 140kb. J1, TK93, K1, PD5, CP1, and
1043 DNAs were digested to 13, 13, 11, 14, 14, and 12 fragments by EcoR1, respectively, and showed its characteristec restriction patterns. Cohesive ends were present in J1, TK93, and
1043, but were absent in K1, PD5, and CP1. Restriction maps of J1 and TK93 DNAs showed nearly complete homology and their evolutionary relationship based upon the restriction analysis was discussed.
Restriction endonuclease maps of three plasmids from bacillus thuringiensis serovar israelensis 4Q1
Faust, R.M. ; ; ; C.L.Meyers-Dowling ; P.E.McCawley ;
The Korean Journal of Microbiology, volume 23, issue 2, 1985, Pages 122~128
Bacillus thuringiensis serovar israelensis 4Q1 contains 8 different covalently closed-circular (CCC) plasmids of molecular weight 204, 267, 109, 103, 16, 7.6, 6.4, and 5.0kb. The three smallest plasmids, designated pBti6, and pBti8 may prove to be useful as cloning vectors because of thier size and ease of isolation. The three plasmids were incubated separately with 9 different restriction enzymes and 7 of the enzymes tested cleaved one or more of the plasmids. Plasmid pBti6 has a single site for Bg1 II, Pst I and Pvu II, two sites for Bc1 I and Eco RI, and five sites for Hind III. Plasmid pBti7 has a single site for Bam HI and Pst I, two sites for Hind III, and three sites for PvuII. Plasmic pBti8 has a single site for Bam HI, BelI and Hind III, two sites for Eco RI, and three sites for Bgl II and Pvu II. Composite restriction enzyme maps for pBti6, pBti7 and pBti8 were constructed. The sites of restriction enzyme cleavage were determined by single, double and partial digests of the plasmid DNA. All the restriction sites were aligned relative to the single Bgl II(pBti6), Pst I(pBti7) or Hind III(pBti8) site, respectively.
Studies on the RK-temperate phage of bacillus cereus
The Korean Journal of Microbiology, volume 23, issue 2, 1985, Pages 129~137
The RK-temperate phage which infected with Bacillus cereus was isolated and the characters were investigated. The induction of RK-temperate phage from host bacterium attained by ultraviolet light irradiation (15W, 30cm, 30-120sec) and mitomycin C treatment (0.2-2 ug/ml). The host range of RK-temperate phage was not revealed with lysogenic and related strains of B. cereus. But B. cereus(PS) 352 which obtained by N-nitrosoguanidine treatment
to phage infected with host bacteria was sensitive bacteria of RK-temperate phage. RK-temperate phage was stabilized at the condition of nutrient broth (pH 7-8), Tris-buffer (pH 7-8) and ammonium buffer (pH 8-9) and Sorensen's phosphate buffer (pH 6-7), but unstabilized at other salt solutions and pH range. Also, thermostability was to
but unstabilized at above
. At RK-temperate phage, the measurment values of head, neck, mid tail and end tail were 59nm,
respectively. The morphology of head was regular polyhedron, and the end tail was coneate form. On the one hand, the number of capsid protein layer of tail were consist of 4, 35, and 1 at neck, mid tail, and end tail, respectively. RK-temperate phage was identified with DNA phage and G+C contents were 38.63. The latent time of RK-temperate phage was 30 minutes and the burst size was 70-80. And the host bacteria was lysed in case of multi-infection, above moi 1.
Expression of Glucose Isomerase Gene from Bacillus licheniformis in Escherichia coli.
The Korean Journal of Microbiology, volume 23, issue 2, 1985, Pages 138~146
A Bacillus licheniformis ATCC31667 gene coding for a glucose isomerase has been cloned and expressed in glucose isomerase negative mutant of Escherichia coli. A recombinant plasmid, constructed by ligation of a EcoRI fragment of B.licheniformis chromosomal DNA to vector plasmid pBR322, was expressed glucose isomerase positive in E.coli LE392-6 with growth on minimal medium containing xylose as a sole carbon source. This recombinant plasmid, designated pBGI6, had the insery of 4.1Kb of Bacillus gene in EcoRI site, and restriction map of the plasmid was established. The plasmid pBG16 was very stable after 10days of serial transfer to a fresh medium. The activity of glucose isomerase from the transformed cell containing pBGI6 was increased about 20 fold than its wild type of host.
Distribution of abiontic carboxymethylcellulase in relation to microbial growth and activity in forest soils
The Korean Journal of Microbiology, volume 23, issue 2, 1985, Pages 147~156
Seasonal and vertical variations of abiontic soil carboxymethylcellulase (CMCase) activities were assessed every other month for a year in two contrasting forest soils and evaluated the relationships between soil CMCase activity and environmental parameters. In climax deciduous soil, variations in CMCase activities caused by differences in sampling time were greater than those caused by differences in soil depth. On the other hand, counter phenomenon was obserned in coniferous soil at the stage of development. Correlation analyses showed that soil CMCase activities were significantly (p>0.01) correlated with microbial respiration rates (
uptake) and all of the microbial population sizes. From these results, it is suggested that determination of abiontic soil CMCase activity is an useful additional index for evaluating the overall microbial growth and activity in soils.
Vertical composition and character analysis of saprophytic bacteria isolated from the mudflat of Nakdong river estuary
The Korean Journal of Microbiology, volume 23, issue 2, 1985, Pages 157~165
Bacterial identification was performed with morphological, physiological and biochemical tests to the isolates from the mudflat of 30cm depth sampled in Nakdong river estuary in March and June, 1985. Flavobacterium and Cnterobacteriaceae were regarded as dominants. Pseudomonas, Bacillus, Micrococcus, Vibrio, Aerococcus, Aerononas, Acinetobacter and Staphylococcus were founded in various depth. Vertical composition of bacterial genera in March was more diversiform than that of June. Character analysis was carried out with the calculation of similarity index (S). At a level of 85% similarity, the isolates were clustered into 5 groups and ungrouped 2 strains. Classifying groups of bacterial strains with determination schemes and groups from similarity index were in good agreement.