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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
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Volume & Issues
Volume 23, Issue 4 - Dec 1985
Volume 23, Issue 3 - Sep 1985
Volume 23, Issue 2 - Jun 1985
Volume 23, Issue 1 - Mar 1985
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The Origin of Ribityl Side Chain of Riboflavin in Ashbya gossypii
The Korean Journal of Microbiology, volume 23, issue 3, 1985, Pages 167~171
In order to investigate the origin of the ribityl froup of riboflavin and the involvement of GTP cyclohydrolase II in the riboflavin pathway, we studied the incorporation of
guanosine using a well known riboflavin over producer, Ashbya gossypii.Cells were grown in a media containing
guanosine and the riboflavin and GMP were isolated and purifired by column chromatography. The isolated compounds, riboflavin and GMP were labeled in the ribityl and ribosyl side chain and the isoalloxazine and guannine moiety. By comparing the specific radioactivity of each compound we reached a conclusion that the ribose of guanosine is converted directly to the rivityl moiety of riboflavin. The results indicate that biosynthesis of the vitamin begins at the level of a guanosine compound and also suppory the involvement of GTP cyclohydrolase II in one of the early steps in the biosynthetic pathway.
Isoenzyme pattern of Aldaline and Acid Phosphatase in the Culture of Saccharomyces uvarum
The Korean Journal of Microbiology, volume 23, issue 3, 1985, Pages 172~176
The present study was designed to investigate isoenzyme (ACPase, ALPase) pattern and its refulatory function between catabolically repressed and derepressed states in yeast, Saccharomyces uvarum. As the results, no other isoenzyme was detectable in acid phosphatase, but there were three isoenzyme types in aldaline phosphatase. Type "B" isoenzyme among alkaline phosphatases in catabolically repressed cell was derepressed, but in normally cultivated cell, type "C" isoenzyme was derepressed while type "B" activity was lowered. Type "B" isoenzyme could be postulated as repressible enzyme, type "A" as constityityve enzyme and type "C" as L-histidinol phosphatase, respectively, Also, it could be shown that type "B" ALPase, repressible enzyme, compensated for phosphate group supplier under catabolically repressed states. Protein profile in cytoplasmic soluble fraction of exponential phase cell was characterized by negative charged protein.
Properties of Extracellular Cytosine Deaminase from Arthrobacter sp. JH-13
The Korean Journal of Microbiology, volume 23, issue 3, 1985, Pages 177~183
Some properties of an extracellular cytosine deaminase produced from Arthrobacter sp.JH-13 were examined after 20-80% of ammonium sulfate fractionation. Among some substrates, this enzyme utilized cytosine and 5-fluorocytosine as a substrate. The optimum pH and temperature for the activity of this enzyme were found to be near 8.0 and
, respectively. The ensyme was more stable in 0.2M of Tris-HCl buffer than 0.2M of potassium phosphate buffer. The enzyme was generally stable below
, but inactivated completely at
. 1mM of
increased the enzyme activity, but 0.01mM of
markedly inactivated the enzyme activity. 0.1mM of p-chloromercuribenzoate, trichloroacetic acid, and N-ethylmaleimide compleyely inhibited the enzyme activity, but 0.1mM of 2-mercaptoethanol slightly increased the enzyme activity.
Characterization of the cured mutants of Lactobacillus casei
The Korean Journal of Microbiology, volume 23, issue 3, 1985, Pages 184~189
The cured mutant strains (CW, CSM, CEM, CAM) we obtained from Lactobacillus casei YIT 9018 spontaneously and by ethidium bromide treatments. The lactose fermenting ability of those mutants was tested and plasmid was found to have some functions in lactose metabolism. Plasmid (PLC) has been detected in Lactobacillus casei YIT 9018, but has not been detected in mutants CSM, CEM, CAM. These plasmid curred mutants showed a decrease in the ability to produce lactic acid from lactose.
Frequency improvement of protoplast fusion in coryneform bacteria
The Korean Journal of Microbiology, volume 23, issue 3, 1985, Pages 190~196
For frequency improvement of protoplast fusion in Brevibacterium flavum, Brevibacterium lactofermentum lactofermentum and Corynebacterium glutamicum, the effect of plasma expanders on fusion and cell wall regeneration, compatison between direct and two-step selection method, tendency of fusion frequency according to pH of fusion fluid and polyethylene glycol concentration were examined. By addition of 3% polyvinyl pyrrolidone to cell wall regeneration medium, regeneration frequencies were expressed 23 (Brevibacterium lactofermentum), 10.4 (Brevibacterium flavum) and 2.7 (Corynebacterium glutamicum) times higher than those of none polyvinyl pyrrolidone medium respectively.
Glycine Effect on Spheroplasting and Nodule Bacteroids of Rhizobium Jjaponicum
The Korean Journal of Microbiology, volume 23, issue 3, 1985, Pages 197~202
Different spheroplasting methods using glycine were tried to fast and slow-growing R. japonicum. Although one of the fast growers, R-271 showed normal growth in the presence of 4mg/ml glycine, cell morphology and colony forming unit (CFU) were greatly different from the cells of late log phase grown in the medium without glycine. In parallel, R-271 became sensitive to lysozyme after 6hr incubation in medium containing glycine (3.5mg/ml). After 24hr cultivation in glycine
medium, one of the slow growers, R-214 was also susceptible to lysozyme action. Spgeroplasting frequency of both strains was over 96% by glycine and lysozyme. Spheroid cell was also found in bacteroids from root nodule and soluble glycine content was relativiely smaller than other amino acids in soybean nodule extracts.
Partial Characterization of Bacillus thuringiensis var. kurstaki Temperature-sensitive Mutants
The Korean Journal of Microbiology, volume 23, issue 3, 1985, Pages 203~208
Partial characterization of B. thuringiensis var. kurstaki 3ab temperature-sensitive mutants was carried out through biochemical analyses, utilization tests of carbohydrate sources, antobiotic resistant test, hemolytic reaction test, growth measurement of Fructus gardenia sxtrant medium and toxicity test against mice. Six ts mutants, ts-U154, ts-U601, ts-U602, ts-U603, tsU-604, and ts-U788 could not produce urease, ts-U603 lost its motility, ts-U154 could not use salicin and cellobiose and ts-U603 not ribose. All ts mutants except ts-U154 and wild type strain were resistant to cephalothin, ampicillin, and penicillin. but ts-U154 was sensitive to the three. Four mutants, ts-U21, ts-U74, ts0U131 and ts-U154 did not form pigment colonies on the F. gardenia medium. All the mutants and wild type strain showed hemolysis reaction on the blood agar. The B. thuringiensis and mutants were not toxic to mice.
Formation of protoplasts from pyricularia oryzae
The Korean Journal of Microbiology, volume 23, issue 3, 1985, Pages 209~214
The optimum conditions of protoplasts formation from Pyricularia oryzae were investigated with lytic enzymes and osmotic stabilizers. The mycelia were begun to refease the protoplasts in response to the complex enzyme solution after 30-60 minutes and reached to maximum after 2-3hrs. Among the lytic enzymes tested, the mixture solution containing
(0.01 ml/ml), Cellulase ONOZUKA-RS(20mg/ml), Driselase (10mg/ml), and Macerozyme R-10 (10mg/ml) resulted in the highest rate of protoplasts releasing of Pyricularia oryzae. The best stabilizer was 0.6M KCl at pH 7.0. Shen the mycelia were digested with enzyme mixture, the stationary culture was better than shaking culture for higher protoplast formation.
Distribution and Activity of Hheterotrophic Bacteria in the Mudflat of Nakdong River Estuary
The Korean Journal of Microbiology, volume 23, issue 3, 1985, Pages 215~222
Distribution pattern and activity of heterotrophec bacteria were measured in the mudflat of Nakdong river estuary. In March and June, 1985, community sizes of amylolytic, lipolytic and proteolytic bacteria as well as total viable counts were measured. Vertical distribution of bacterial community size increased a few orders of magnitude from Narch to June. Heterotrophic activity was estimated in turnover time with
. Turnover time reduced considerably in June compared to that of March. To sxamine correlations for measured bacterial groups, turnover time and environmental factors, correlation coefficient matrix was obtained. These measured characteristics did not consistently correlate well with one another.
Isolation and Bacteriological Characteristics of Spiral form Bacteria from Patient with Epidemic Pulmonary Hemorrhagic Fever
The Korean Journal of Microbiology, volume 23, issue 3, 1985, Pages 223~229
We have previously isolated seven strains of spiral form bacteria, which have shown several similar characteristics to Leptospira from patient with epidemic pulmonary hemorrhagic fever and matural paddy water. Further studies have performed to characterize them. All of the isolated bacteria were aerobic, gram negative and spiral form and their growth were completely inhibited at temperature below
. In sensitivity test to drugs, the bacteria were resistant to the bile salts, 5-Fu and Amphotericine B, but sensitive to the 8-Azaguanine. The isolated bacteria as well as 5 serotypes of Leptospira interrogans reacted the patient sera and it was also shown that antisera from rabbit immunized with the isolated bacteria reacted with 5 serotypes of Leptospira interrogans. These results suggest that the isolated bacteria may belong to the genus Leptospira.
Isolation of a Malonate-utilixing Acinetobacter calcoaceticus from Soil
The Korean Journal of Microbiology, volume 23, issue 3, 1985, Pages 230~234
A bacterium which can utilize malonate as a sole carbon source was isolated from soil. This strain was identified to be Acinetobacter calcoaceticus by morphological, cultural, phtsiological and biochemical examination. When this microorganism was grown on malonate as a aole carbon source, the enzymes, such as malonyl-CoA synthetase, isocitrate lyase and malate synthase were induced. These results suggest that in this microorganism, malonate is also assimilated through the proposed pathway in Pseudomonas fluorescens:
Numerical Classification of Phototrophic Nonsulfur Bacteria
The Korean Journal of Microbiology, volume 23, issue 3, 1985, Pages 235~240
A total of 10 main characters of 16 species of family Rhodospirllaceae were phenetically and cladistically analyzed by Farris' method. The obtained phenogram and cladistic tree were compared with Bergey's manual and other papers. The results supported that the system of 5 subgroups (genera) is available in family Rhodospirllaceae and indicated that close affinities between Rhodospirllum tenue and Rhodopseudomonas gelatinosa and between Rhodomicrobium vannielii and other species of genus Rhodopseudomonas were proved.