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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 23, Issue 4 - Dec 1985
Volume 23, Issue 3 - Sep 1985
Volume 23, Issue 2 - Jun 1985
Volume 23, Issue 1 - Mar 1985
Selecting the target year
Purification and Characterization of the Regulatory Substance of Furfural Biodegradation in Pseudomonas fluorescens
The Korean Journal of Microbiology, volume 23, issue 4, 1985, Pages 241~247
The objectives of this study were to isolate and identify ninhydrin positive substande(s) produced in the culture broth of Pseudomonas fluorescens. It was found that the NPS could stimulate bioconversion of furfural into furoic acid. In order to isolate the NPS from the culture broth, cell free filtrate was subjected to ion-exchange chromatography, gel-permeation and finally to cellulose column chromatography. The purified NPS was white amorphous power and very soluble in water, slightly soluble in methanol and very insoluble in organic solvents. UV, and IR absorption spectra.
were measured in order to identify the chemical structure of the NPS.
Characteristics of an imperfect fungus, Microsporum nigricans Yeeh sp. nov.
The Korean Journal of Microbiology, volume 23, issue 4, 1985, Pages 248~251
An imperfect fungus recognized as a dermatophyte was isolated from soil samples and the macroscopic, microscopic and physiological characteristics were examined. From the comparisons of this isolate with the known species, the strain belonged to the genus Microsporum. She showed mant different characteristics from the related species, including colony color and shape, or number in a cell of macrocondium. This isolate was considered to be a novel species in the genus Microsporum. Therefore the name was given to her as Microsporum nigricans Yeeh sp. nov. because of dark blackish color from the macroscopic observation.
Effect of Cultural Conditions on the Lipid Production by Moulds
The Korean Journal of Microbiology, volume 23, issue 4, 1985, Pages 252~258
Aspergillus niger var. macrospours, Aspergillus fumigatus, Penicillium notatum and Penicillium spinulosum were cultured under different cultural conditions. The lipids produced by these species and fatty acid compositions of lipids were investigated. The macimum lipid contents produced by each species were 17.8% for Asp. niger var. macrosporus, 31% for Asp. fumigatus, 12.6% for P. notatum and 17.5% for P. spinulosum, respectively. The major fatty acid compositions were palmitic acid, stearic acid, oleic acid and linoleic acid. Asp. niger var. macrosporus and Asp. fumigatus were highest oleic acid during all incubation periods and P. notatum and P. spinulsum were linoleic acid. Degree of unsaturation was higher Penicillium than Aspergillus. The fatty acid compositions were changed depending on the incubation temperature, but hardly showed a certain tendency except linoleic acid and degree of unsaturation that were higher at lower temperature.
Effects of crude Saponin on growth and Aflatoxin production by Aspergillus parasiticus
The Korean Journal of Microbiology, volume 23, issue 4, 1985, Pages 259~264
The research was carried out for the purpose of finding effects of gerbal saponins on aflatoxin synthesis by Aspergillus parasitics NRRL 2999. A. parasiticus with
conidia were grown at
for 9 days on the enriched medium that is optimum for the frowth and aflatoxins production by the mold. The inhibitory effect on the growth and aflatoxins produced by the mold occurred in the presence of 0.36% of crude red-ginseng saponin showing both the growth and aflatoxins production come to 62.3% (growth), 38.7% (aflatoxin
) and 22.9% (aflatoxin
) of the control. Thd next effective saponin to inhibit the growth and aflatoxins production was from burdock seeds. However, saponin extracted from honeysuckle flowers had no inhibitory effect. The mold caused no changes in the pH of the medium when it contained red-ginseng saponin. Red-ginseng saponin was more effective than the white-ginseng in inhibiting both the growth and aflatoxin production.
Electron Microscopic Observation on Protoplast Fusion of Coryneform Bacteria
The Korean Journal of Microbiology, volume 23, issue 4, 1985, Pages 265~270
Overall procedure of cell fusion between Brevibacterium flavum and Corynebacterium glutamicum was morphologically observed by transmission electron microscopy. Protoplasts formed by treatment of cells with penicillin G and lysozyme in order were released through the pores generated on a certain region of cell walls to be spherical form. When two different protoplasts were met, cell wall and membrane in the contact zone was disappeared and followed by the mutual exchange of cytoplasmic and/or chromosomal materials. Cell xall regeneration speed of the protoplasts fused was slower than that of the non-fused, whereas the size of the former was confirmed as bigger than that of the latter.
Purification and partial characterization of bacillus thuringiensis var.thuringiensis exotoxin
The Korean Journal of Microbiology, volume 23, issue 4, 1985, Pages 271~281
Bacillus thuringiensis var. thuringiensis produces an extracellular insecticidal thermostable .betha.-exotoxin, which was purified through microfiltering, barium precipitation, charcoal absorption chromatography, ion exchange column chromatography and gel filtration. The exotoxin in each purification step was detedted by thin layer chromatography, high pressure liquid chromatography and paper electrophoresis with efficient results. The exotoxin productivity on time course was checked by spectrophotometric absorbance at 258nm with the result that the exotoxin was initially produced in 6 hour culture and reached maximum value in 36 hour culture. Anti-bacterial effect test on Micrococcus flava was applied as toxicity test. The results showed that frowth inhibition of M. flava could be shown in plate assay of cell free filtered supernatant, alkaline eluant from charcoal and purified exotosin obtained from gel filtration column chromatography on Sephadex G-10 appeared to be 740. Heat stability of the exotoxin was confirmed through autoclaving twice.
R-plasmids in staphylococcus aureus
The Korean Journal of Microbiology, volume 23, issue 4, 1985, Pages 282~290
Small size antibiotic resistance plasmids having molecular weights less than 10 Mdal were isolated and characterized from ten clinically isolated multiple resistant Staphylococcus aureus. Agarose gel electrophoresis profiles and antibiotic resistance patterns divided these strains into four groups. Strain 2-23-6, the representative strain of a group of five strains conferred two plasmids of molecular weights
dal. The small plasmid (pSBK 112) specified macrolides, lincosamides and streptogramin type B (MLS) resistance gene which are expressed constitutively. Lage plasmid (pSBK 125) specified chloramphenicol resistance gene which is inducible. Strain 10-5 conferred a
dal plasmid (pSBK 141) which carry an inducible ampicillin resistance gene and strain P-H-2 conferred and
dal plasmid (pSBK 190) which carry a constitutive MLS resistance gene. Strain D-H-1 conferred four plasmids of molecular weights
dal (pSBK 201),
dal (pSBK 202),
dal (pSBK 203), and
dal (pDBK 204), respectively. Among those four plasmids, only pSBK 203 specified chloramphenicol resistance gene. Curing of constitutive MLS resistance using acriding orange or ethidium bromide in 2-23-6 and P-H-2 strains produced 'inducible' MLS resistance strains which are less resistant to MLS than the wild type strains, suggesting that there are two resistance genes in both strains; one is constitutive and the other is inducible.
Animal Pathogenesis and Specificities of Leptospira (Korea) with Autopsied Human Tissue
The Korean Journal of Microbiology, volume 23, issue 4, 1985, Pages 291~296
Leptospira isolated from patients and natural paddy water were further studied to confirm their serologic specificity with the bacteria in infected animal tissue and autopsied tissue of patients died with leptospirosis. And pathologic patterns of the inoculated antimal and the virulence of the bacteria in the animals were also studied. The findings are summarized as follows;
dose of the bacteria in guinea-pig were
cells and mice were found to be susceptible to them even though the degree of susceptibilities were much inferior to guinea pig. The bacteria were recovered from various organs; demonstrated massive hemorrages due to diapedesis and monocyte infiltration were observed, in some cases, intramedullary hemorrages of the infected kidnies and hematuria were recognized. All of the hyperimmune sera were strongly reacted with the tissues from autopsied human case who died of EPHF(Epidemic Pulmonary Hemorrhagic Fever) in immunofluorescent antibody tests.
Steroid modification with aspergillus phoenices
The Korean Journal of Microbiology, volume 23, issue 4, 1985, Pages 297~301
The dependence of activities of Aspergillus phoenicis on the culture conditions in the progesterone transformation reaction was investigated. In the beginning of the reaction,
was not produced even at high concentration of
. However, large amount of the product was obtained after the complete exhaustion of progesterone. When spores of A.phoenicis replaced mycelia as enzyme source,
was produced after a considerably long indyction period, and its maximum production rate followed the exponential growth phase. The
continued, even after the stationary growth phase. A. phoenicis showed high enzyme activity for these reactions when the phosphate buffer solutions were used in place of the ordinary culture medium. The buffer solutions of low pH gave more yield of
than those of high pH. However, the addition of flucose to the buffer solutions did not activate the transformation reaction. The presence of progesterone seems to be necessary for the induction of enzymes for the
is not produced in the reaction medium containing only
as a substrate.
A Possible Enzymatic Catabolism of L-Ascorbic Acid via
The Korean Journal of Microbiology, volume 23, issue 4, 1985, Pages 302~308
It is shown by means of nuclear magnetic resonance spectroscopy that 3,4,5-trihydroxy-2-keto-L-valeraldehyde (L-xylosone), an
-ketoaldehyde, is formed during the oxidative catabolism of L-ascorbic acid. It is proposed that this substance serves as a substrate for the glyoxalase system by which it is transformed to L-xylonic acid. As L-xylonic acid is further oxidized to L-erythroascorbic acid, a biochemical pathway is proposed for the action of vitamin C which consists of two further
-lactones and three different substrates of the glyoxalase system.
Genetic Recombination by Protoplast Fusion of Cellulomonas sp CS 1-1
The Korean Journal of Microbiology, volume 23, issue 4, 1985, Pages 309~314
Autoxotrophic mutants of a cellulolytic baterium Cellulomonas sp. CS 1-1 were grown at
for 6hr using a complete medium containing 0.5M sucrose and for another 90 min after addition of 0.3 U/ml penicillin G, and were protoplasted by 0.2mg/ml lysozyme for 2hr. Prototrophic recombinants were obtained at the rates of
by fusing the protoplasts in the presence of 40% polyethyleneglycol3350. Nystatin could be used to eliminate fungal contamination during the regeneration of the plotaplasts.