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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
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Volume & Issues
Volume 24, Issue 4 - Dec 1986
Volume 24, Issue 3 - Sep 1986
Volume 24, Issue 2 - Jun 1986
Volume 24, Issue 1 - Mar 1986
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Studies on the Oranization and Expression of tRNA Genes in Aspergillus nidulans (V) The Molecular Structure of
in Aspergillus nidulans
The Korean Journal of Microbiology, volume 24, issue 2, 1986, Pages 79~85
We have determined the sequence of
of A. nidulans partially by enzymatic rapid RNA sequencing technique. The sequence was 5'GGCCGGCUGGCCCAAXUGGCAAGGXUCUGAXUACGAAXCAGGAGAUUGCACXXXXXGAGCXXUXXGUCGGUCACCA3' The cloverleaf structure was made from above data. As a result, the anticodon sequence was identified as ACG. This result was confirmed with charging test. The complete sequence was proposed by supplementing the DNA sequence to and by assigning the position of minor bases to this RNA sequence.
Inhibition of Sma I, Ava I, Nae I, and Xma I endonuclease activities by the methylation of DNA with Hpa II methylase
The Korean Journal of Microbiology, volume 24, issue 2, 1986, Pages 86~90
The DNA methylated by Hpa II methylase was not cleaved by Sma, I, Ava I and Nae I endonucleases. This experimental data could be interpreted as strong evidences that Sma I, Ava I and Nae I methylases which yet to be isolated would methylate on the inmost cytosine nucleotide within their hexameric recognition sequences. The facts that Sma I, Ava I and Nae I endonucleases can not cleave the DNA methylated by Hpa II methylase are the valuable informations for protecting DNAs upon cleavage reactions by Sma I, Ava I and NAe I endonucleases especially for cDNA insertion experiments into vector DNAs using Sma I, Ava I and Nae I oligonucleotide linkers. In the case of Xma I endonuclease, partially cleaved DNA fragments were observed although the reaction rate was greatly decreased. This result implies that the methylation site of Xma I methylase which yet to be isolated would not be the same as that of Hpa II methylase in Xma I sequence.
Interspecific protoplast fusion of trichoderma koningii and trichoderma reesei
The Korean Journal of Microbiology, volume 24, issue 2, 1986, Pages 91~97
Intra and interspecfic fusants were produced by the protoplast fusion of auxotrophic mutants from Trichoderma koningii ATCC 26113 and Trichoderma reesei QM 9414. It was found that 0.6M
was the best osmotic stabilizer for the preparation of protoplasts from the mycelium of T. koningii and T. reesei respectively. However,
was the most suitable one for the regeneration of the protoplasts from both species. The intraspecific protoplast fusion frequencies between the auxotrophic mutants from T. reesei were
. Interspecific protoplast fusion frequencies between the auxotrophic mutants from T. koningii and T. reesei were
. Interspecific complementing fusants, however, were not alwats produced. Fusants obtained from interspecific potoplast fusion were spontaneously segregated into various strains including parental types, non-parental auxotrophic hybrids, and prototrophic hybrids on complete plate. Interspecific hybrids revealed to have partially enhanced celluloytic activities.
Rhizobium meliloti 102F51 Mutants Defective in Heme Synthesis
The Korean Journal of Microbiology, volume 24, issue 2, 1986, Pages 98~105
Rhizobium meliloti 102 F 51, the symbiotic partner of alfalfa, was mutagenized with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) and UV-irradiation. Three group of mutants which form white, white-pink and red nodules were selected. The adetylene reduction activity, nodulation activity, amount of heme synthesis during the nodulation, and
acid synthetase (ALAS) and
acid dehydratase (ALAD) activities in free living rhizobia and bacteroid states of the each group of mutants were compared. The mutants forming white nodules showed lower acetylene reduction activity compared to those of red nodule forming mutants. The two key enzymes for the heme synthetic pathway, ALAS and ALAD activities of the mutants forming red nodules was much higher than those of the mutants forming white nodules in bacteroid state, however no significant difference was observed in free living state. In the nodules the ALAS was detected only in bacteroid fraction, while ALAD was detected both in bacteroid and plant fraction. ALAS was dramatically increased with the heme synthesis during the nodulation, while ALAD was decreased in plant fraction but slight increase was observed in bacteroid fraction.
Isolation of Salicylate-Degrading Plasmid from Pseudomonas putida
The Korean Journal of Microbiology, volume 24, issue 2, 1986, Pages 106~112
The large plasmid (about 180 megadaltons) was isolated from the aquatic strain of Pseudomonas which was found to degrade salicylate. It was found that the plasmid could be isolated under gentle conditions in comparison with other methods. The yield of covalently closed circular DNA was enganced by heat treatment at
after denaturing the chromosomal DNA with alkaline sodium dodecyl sulfate (pH 12.45), and the plasmid DNA was selectively concentrated by utilizing 10% polyethylene glycol as final concentration. It was also found that the cured strains with mitomycin C did not show any growth on the medium containing salicylat6e, therefore, it was concluded that the plasmid might play and important role on the salicylate degradation.
Role of glutamine synthetase as as regulator of nitrogenase in rhodopseudomonas sphaeroides D-230
The Korean Journal of Microbiology, volume 24, issue 2, 1986, Pages 113~118
Optimum temperature and pH of glutamine synthetase activity (E.C. 184.108.40.206.) of R. sphaeroides D-230 was
and 6.8, respectively. The adenylated state of GS in R. sphaeroides D-230 was stabilized by addition of 0.2mg/ml of cethyltrimethylammoniumbromide. Valine, histidine, proline, isoleucine, and lysine were good nitrogen source for the growth of R. sphaeroides D-230. The growth of R. sphaeroides D-230 in
as sole nitrogen source was lower than in any otherculture conditions. GS activity was inhibited, more or less, by various amino acid. THe relative inhibition rate of the enzyme by added 7mM arginine,
was 63.8%, 26.79%, 6.24%, and 10.64%, drespectively. THe hydrogen evolution of R. sphaeroides D-230 grown in N-limited media was inhibited by 0.1mM MSX, irreversible GS inhibitor. GS activity was completely inhibited by 1.0mM MSX but ammonia released maximally at the same concentration of MSX. Ammonia release by added MSX was increased up to 1.0mM MS, but decreased above 1.0mM MSX. It is probably due to inhibition of nitrogenase actixity by MSX. Nitrogenase activity was not inhibited at low concentration of MSX. These results suggests that the inhibition of nitrogenase activity by ammonia is mediated by products of ammonia assimilation rather than by ammonia itself.
Isolation and properties of protease Pi in escherichia coli
The Korean Journal of Microbiology, volume 24, issue 2, 1986, Pages 119~126
A periplasmic endoprotease, named protease Pi, was purified to homogeneity from Escherkchia coli by conventional procedure with insulin as substrate. This enzyme degrades insulin and glucagon to trichloroacetic acid-soluble meterials, but shows little or no hydrolysis of bovine serum albumin, casein or globin. Its molecular weight was 110, 000 when determined by gel filtration on Sephacryl S-300 and was 105, 000 when estimated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Thus, it appears to be single polypeptide. This snzyme is metalloprotease, since it is completely inhibited by o-phenanthroline and can be activated by addition of divalent metal cations, such as
. It is destinct from protease Ci, a cytoplasmic insulin degrading enzyme, since protease Pi is localized to the periplasm. Since protease Pi selectively degrades GTP cyclohydrolase I, it appears to play a role in the regulation of pteridine biosynthesis.
Asymbiotic nitrogen fixation of R. japonicum in soybean nodule extract
The Korean Journal of Microbiology, volume 24, issue 2, 1986, Pages 127~132
Soybean nodule extract was prepared and tested for the effectiveness in the induction of asymbiotic nitrogen fixation of R. japonicum P-168. A Asymbiotic nitrogenase activity was increased over twice when glutamate was replaced by nodule extract in the induction media. Independently of the induction media, the nitrogenase activity in the assay media was also enhanced by the addition of nodule extract (
protein/ml). The amount of ethylene in the assay media reached the highest point after 8 days incubation of R-168 and was decreased thereafter. The growth of R. japonicum R-168 was sensitive to the concentration of nodule extract. As a while, the effect of soybean root extract was not detected both in the induction of nitrogenase activity and in the growth of R. japonicum R-168.
Carbon Monoxide Dehydrogenase in Cell Extracts of an Acinetobacter Isolate
The Korean Journal of Microbiology, volume 24, issue 2, 1986, Pages 133~140
Extracts of CO-autotrophically grown cells of Acinetobacter sp. 1 were shown to use thionin, methylene blue, or 2,6-dichlorophenol-indophenol, but not NAD, NADP, FAD, or FMN, as electron acceptors for the oxidation of CO under strictly anaerobic conditions. The CO dehydrogenase (CO-DH) in the thes bacterium was found to be an inducible enzyme. The enzyme activity was determined by an assay based on the CO-dependent reduction of thionin. Maximal reaction rates were found at pH 7.5 and
, and the Arrhenius plot revealed an activation energy of 6.1 kcal/mol(25.5kJ/mol). THe
m/ for CO was
. Known metalchelating agents tested had no effects on the CO-DH activity. No divalent cations tested affect the enzyme activity significantly escept
which suppressed the activity completely. The enzyme was inhibited by glucose and succinate. The same extracts catalyzed oxidation of hydrogen gas and formate with thionin as electron acceptor. The CO-DH of Acinetobacter sp. 1 was to have no immunological relationship with that of Pseudomonas carboxydohydrogena.
Properties of Extracellular Polyphenol Oxidase Isolated from Lentinus edodes JA01
The Korean Journal of Microbiology, volume 24, issue 2, 1986, Pages 141~146
To find the role of polyphenol oxidase in lignin biodegradation, chracteristics of extracellular polyphenol oxidase activity from Lentinus edodes JA01 was investigated. Polyphenol oxidase had its optimum activity at pH 4.5 and
respectively. Also, the enzyme was very unstable in various pHs and comparatively heat stable up to
salts medium, the growth rate of L.edodes JA 01 was relatively slow and polyphenol oxidase activity appeared 2 and 14 days after inoculation. No significant relationships were found between polyphenol oxidase activity and the amounts of lignosulfonate present in the culture medium.
Assessment of Mycobacterial Viability by Fluorospectrophotometry
The Korean Journal of Microbiology, volume 24, issue 2, 1986, Pages 147~153
Viable potential of Mycobacterium smegmatis, a slow grower in vitro cultivation and of M. leprae, an obligate intracellular parasitic bacterium, which can not be cultured yet in vitro was assessed by fluorospectrophotometry. Bacterial cells in different numbers and under various physiological status were incubater with fluorescein diacetate(FDA). After an incubation of the bacterial preparations with FDA at specified conditions, amount of fluorescein inside bacteria was measured by a fluorospectrophotometer at 470nm and 510nm of excitation and emission wavelengths, respectively. Fluorounit given by such bacteria showed a correlation with assessment of viability of the same preparations made by other methods, such as optical density and colony forming units of M. smegmatis and intracellular ATP content of M. leprae. The possible use of fluorospectrophotometry in assessing viability or physiological potential of bacteria, particularly intracellular parasites and fastidious organisms to culture in vitro is discussed in relation to other methods.
Interspecific Variation in the Protoplast Formation of the Genus Cellulomonas
The Korean Journal of Microbiology, volume 24, issue 2, 1986, Pages 154~160
In order to develope interspecific fusion of the genus Cellulomonas capable of assimilation cellulose, the optimun conditions for the protoplast formation was investigated to examine the susceptibility of cell wall, between different species of the same genus using scanning electron microscope. The variation in the susceptibilities of Cellulomonas sp. CS 1-1 and C. flavigena to lysozyme treatment were considerably remarkable, although they belong to the same genus. The rate of protoplast formation of CS1-1 was 99.9% being treated with lysozyme
for 30 minute and that of C. flavigena was about 80% being treated at the concentration of
of lysozyme for 6 hours. The susceptibility of cell wall to the lysozyme treatment on protoplast formation of the strain, CS1-1 seems not to be depend on the cultural periods of cells. On the contrary, that of C. flavigena was considerably depend on the periods. Cells of C. flavigena at mid exponential phase could be more efficiently converted to protoplast cells than those at late exponential phase be done. The rate of the protoplast formation was 95%, when cells of C. flavigena at mid exponential phase were treated with lysozyme
for 6 hours and observed by SEM. In the evalution of protoplast formation of the CS1-1 results of counting method in plate after osmotic shock treatment were similar to the results of the direct observation method by means of SEM. But in the case of C. flavigena the latter method was much more reliable than the former, because the differences between the number of spheroplasts and protoplasts were not able to figure out on conuting the number of protoplast after osmotic shock tretment.
Physiological characterization of SP816 bacteriophage
The Korean Journal of Microbiology, volume 24, issue 2, 1986, Pages 161~167
Some of the physiological properties of Sp816 bacteriophage of Bacillus subtilis SNU816 were characterized. It could form plaques on either B. subtilis SNU816 or B. natto 8102, but not on any other bacillus strains investrgated. Its plaque morphology was circular with a diameter of less than 1.0mm and had a narrow halo surrounding the clear center. Its latent period was 34-36 min and had a burst size of 547. It was most stable at pH 6.0, and rapidly inactivated at
with a initial deaty rate of -0.216
. Host range, thermal inactivation rate at
, pH stability, and UV sensitivity revealed that SP816 was quite different from any other phages investigated together but seemed to be rather related to B. natto phages.
Effects of Phenolic Compounds in Milled Barley Grains on the Growth of Saccharomyces cerevisiae
The Korean Journal of Microbiology, volume 24, issue 2, 1986, Pages 168~174
The phenolic compounds contained in milled barley grains were seperated and identified by gas liquid chromatography and the effects of phenolic compounds extracted from milled barley grains and each authentic phenolic compound on the growth of Saccharomyces cerevisiae were studied. Severn phenolic acids, namely cinnamic, protocatechuic, ferulic, sinapid, vanillic, syringic, gallic acids, were identified in milled barley grains by gas liquid chromatography. The contents of sinapic, ferulic, cinnamic, protocatechuic acids were larger than those of vanillic and gallic acids. Phenolic compounds, extracted from milled barley grains and supplemented in culture broth, were inhibitory to the growth of Saccharomyces cerevisiae at levels above 100ppm to 24 hours but not inhibitory at all levels after 48 hours. Cinnamic, ferulic, vanillic acids at all levels were inhibitory to the growth of Saccharomyces cerevisiae, among them cinnamic acid was most inhibitory. Syringic acid was inhibitory to the growth of the yeast at the initial stage of culture. But sinapic and protocatechuic acids were slightly stimulatory to the growth of the yeast and gallic acid was ineffective to the growth of the yeast.
Characterization of bacillus thuringiensis isolate HL-15 from Korean soil
The Korean Journal of Microbiology, volume 24, issue 2, 1986, Pages 175~183
The isolate HL-15 of Bacillus thuringiensis had common biochemical characteristics of the 23 serovarieties of B. thuringiensis. The isolates formed round endotoxin crystals which killed insect larvae, showed independent serological H antigen to the 23 serotypes, and contained two defferect DNA elements with over 100 Mdaltons of molecular weights.
Environmental factors and the distribution of soil microorganisms in ginseng field
The Korean Journal of Microbiology, volume 24, issue 2, 1986, Pages 184~193
Interrelation between environmental influences on soil microorganisms and it's effect on disease development in ginseng (Panax ginseng C.A. Meyer) field were studied to obtain a preventive measures against the root rot of ginseng caused by soil-borne pathogens in soil in three major Korean ginseng producing areas such as Kumsan, Goesan and Poonggi. Populations of actinomycetes were relatively high in fall season from September to November. Their numbers were highly populated in healthy plot in field than replanted disease field of ginseng, whereas ratio of Trichoderma spp to actinomycetes increased in healthy plot of field indicating the higher numbers of Trichoderma spp pressented in healthy plot field. The numbers of propagules of Trichoderma spp generally increased in early summer through early fall season. Their numbers were also highly populated in the healthy plot of fields. The contents of organic matter and phosphate in healthy plot of field were somewhat high, and phophate/organic matter ratio and Mg content were high in diseased replanted field. All of the soil samples showed a weak acidic pH from 4.5 to 4.7. Soilmoisture content was increased during winter season and it did not show any significant changes curing the growing period, showing 24.6% in healthy plot in field and 19.5% in deseased plot in field respectively. Soil temperature was highest in July and August and lowest in January and February.
The effect of dibutyryl cyclic adenosine 3', 5'-monophosphate on induction of malonate kinase and isocitrate lyase in acinetobacter calcoaceticus
The Korean Journal of Microbiology, volume 24, issue 2, 1986, Pages 194~197
Malonate kinase and isocitrate lyase were induced in Acinetobacter calcoaceticus grown on malonate as a sole carbon source but repressed by succinate. The induction of those two enzymes was stimulated by dibutyryl cyclic adenosine 3', 5'-monophosphate, indicating that the expression of their genes for those enzymes is dependent on cyclic adenosine 3', 5'-monophosphate.