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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 24, Issue 4 - Dec 1986
Volume 24, Issue 3 - Sep 1986
Volume 24, Issue 2 - Jun 1986
Volume 24, Issue 1 - Mar 1986
Selecting the target year
-glucosidase of aspergillus nidulans
The Korean Journal of Microbiology, volume 24, issue 4, 1986, Pages 335~340
Two kind of
, tightly-bound enzyme(TBE) and loosely-bound enzyme(LBE), were obtained from the conidia of Aspergillus nidulans. The existence of enzymes in conidia was conformed by the fact that these enzyme activities were proportional to the number of conidia. The levels of enzyme activities were independent of aging of the conidia. Enzymes were characterized partially. In spite of the physical and chemical treatments of conidia, there was no significant change in TBE activity. The optimum pH and temperature was 6.0 and
, respectively. Thermostability of the TBE was remarkably higher than that of mycelial
. The electrophoretic pattern of LBE was identical to that of mycelial
. These results suggest that conidial
are involved in adaptation process of the conidia to variable environments.
Studies on antibiotics resistance gene in Staphylococcus aureun Plasmid: Cloning of chloramphenicol resistance determinant
The Korean Journal of Microbiology, volume 24, issue 4, 1986, Pages 341~351
R-plasmid(pSBK203, 2.5Mdal) conferring chloramphenicol resistance was isolated from mutiple antibiotic resistant Staphylococcus aureus D-H-1. Bacillus subtilis BD170 was transformed by this plasmid and restriction enzyme clevage sites of this plasmid were mapped for the cloning of chloramphenicol resistance gene. Taq I partial digested fragment of pSBK203(1.3kb) inserted into Cla I site of pBD9 appears to have both regulatory region for induction and structural gene for chloramphenicol resistance whereas Rsa I fragment (1.3kb, both ends are staggered away 0.1Kb from those of Taq I fragment) inserted into Sca I site of pBR322 showed constitutive expression in E. coli. Hinf I, Taq I, and Bgl II restriction enzyme recognition sites are found in both Rsa I fragment and Taq I fragment. Among these, Bgl II recognition site was associated with chloramphenicol resistance.
Effects of Ginseng Saponin and Its Related Materials on Aflatoxin Production by Aspergillus parasiticus NRRL2999 in Synthetic Medium
The Korean Journal of Microbiology, volume 24, issue 4, 1986, Pages 352~356
A study was carried out to determine the effect of ginseng saponin an its related materials on aflatoxin production by Aspergillus parasiticus NRRL2999 in glucose-salts(GS) medium. Maximal growth of the mold and AF froduction in the medium occurred after 5 and 9 days at
, respectively. When various concentrations of saponin added to the medium aflatoxin synthesis were significantly reduced (p<0.05) compared to the control after 9 days at
. 0.05% of saponin inhibited aflatoxin production most effectively in the low concerntrations of saponin (0.01-0.2%) and the toxin synthesis reduced with an increasing concentrations of saponin in the high concentrations (0.03-5.0%). Various concentrations (0.01-1.0%) of saponin diol and triol in the media also caused to reduce aflatoxin synthesis by the mold (p<0.05). All saponin fractions were found to decrease aflatoxin production significantly. Saponin fraction numbers of 1,2,4 and 6 were shown to reduce aflatoxin production effectively, and the number 1 was the most effective. Addition of 0.05% of nucleic acid related materials to the medium reduced aflatoxin production (p<0.05). Aflatoxins could not be found in broth at all, but in mycelia when 0.05% of caffeine was added to the medium. Aflatoxin synthesis was well correlated with total lipid synthesis, growth and glucose uptake. When aflatoxin synthesis inhibited (5.0% of saponin) both total lipid synthesis and growth were stimulated and the efficiency of glucose utilization was reduced.
Molecular cloning and restriction endonuclease mapping of homoserine dehydrogenase gene (HOM6) in yeast saccharomyces cerevisiae
The Korean Journal of Microbiology, volume 24, issue 4, 1986, Pages 357~363
Synthesis of threonine and methionine in yeast, Saccharomyces cerevisiae shares a common pathway from aspartate via homoserine. HOM6 gene encodes homoserine dehydrogenase (HSDH) which catalyzes the inter-conversion of beta-aspartate semialdehyde and homoserine. The level of HSDH is under methionine specific control. A recombinant plasmid (pEK1: 13.3kb), containing HOM6 gene, has been isolated and cloned into E. coli by complenemtary transformation of a homoserine auxotrophic yeast strain M-20-20D (hom6, trp1, ura3) to a prototrophic M20-20D/pEK1, using a library of yeast genomic DNA fragments in a yeast centromeric plasmid, YCp50(8.0kb). Isolation of HOM6has been primarily confirmed by retransformation of the original yeast strain M20-20D, using the recombinant plasmid DNA which was extracted from M20-20D/pEK1 and subsequently amplified in E. coli. Eleven cleavage sites in the insery (5.3kb) have been localized through fragment analysis for 8 restriction endonucleases; Bgl II(2 site), Bgl II(1), Cla I(3), Eco RI(1), Hind III(2), Kpn I (1), Pvu II(1) and Xho I(1).
Protoplast Fusion of Streptomyces Tubercidicus
The Korean Journal of Microbiology, volume 24, issue 4, 1986, Pages 364~369
A procedure for the preparation, regeneration and fusion of protoplasts of Streptomyces tubercidicus was confirmed. Also, protoplast releasingprocesses from mycelia were observed by scanning electron microscope. Three types of protoplasts releasing processes-from the hyphal tip, hyphal end regions and lateral regions of the hyphae-were observed. More than 17% regeneration efficiency was obtained by regeneration medium that is composed of tryptone-yeast extract-sodium acetate-
-sucrose. Optimal concentrations of
and sucrose in the regeneration medium were 50mM, 0.4-0.5M respectively. Above 30% of fusion frequency of the protoplasts derived from two auxotrophic strains of S. tubercidicus was induced by polyethylene glycol 4000(60% w/v).
Partial Purification of the Outer Membrane-Associated 2-Furaldehyde Dehydrogenase from Klebsiella pneumoniae
The Korean Journal of Microbiology, volume 24, issue 4, 1986, Pages 370~376
From the outer membrane portion of Gram-negative Klebsiella pneumoniae, the activity of 2-furaldehyde dehydrogenase depending upon beta-nicotinamide adenine dinucleotide was detected. Cytoplasmic membrane was preferentially extracted from crude membrane with
and Triton X-100, and then outer membrane was collected by ultracentrifugation. The crude enzyme was obtained by solubilization of outer membrane with lysozyme, ethylene diamine tetraacetate and Triton X-100. Thereafter 2-furaldehyde dehydrogenase was partially purified through column chromatography on QAE-Sephadex Q-50 and Sephadex G-150 and the enzyme activity was analyzed by means of high performance liquid chromatography. The optimal pH for the activity of the enzyme was about 9.5 and the optimal temperature was about
. The partially purified enzyme retained tis activity at
for 5 hours. The optimal concentration of Triton X-100 for the activity of the enzyme was about 1.5% in the reaction mixture.
Serological activity of fractions of mycobacterial antigens
The Korean Journal of Microbiology, volume 24, issue 4, 1986, Pages 377~384
A study on the production of mycobacterial antogens has been made in order to improve immunological reactivity and specificity, which have long been explored for the better use of immunological diagnosis of mycobacterial infections. Instead of culture filtrate cell extract was used as a starting material for the production of antigens in this study. Cell extract was fractionated though several steps such as salting out, gel filtration and ion exchange column chromatography and reactivity and specificity of the fractions so produced were enaluated by the various serological methods. The result showed that the species-specific antigenic components distributed mostly in the fractions, Tc of M. tuberculosis, Kc of M. kansasii, Sa of M. scrofulaceum, Aa of M. avium and Fa, Fb, Fc (FF1) of M. fortuitum, which were fractionated by ion exchange column prior to concentrating by salting out and molecular sieving.
The Influence of Kudzu Root Starch on the Growth and Metabolism of Baker's Yeast During Aerobic Semi-Solid Fermentation
The Korean Journal of Microbiology, volume 24, issue 4, 1986, Pages 385~388
In a study of the aerobic growth of Baker's yeast (Saccharomyces cerevisiae) on Maxon-Johnson medium (with glucose as substrate) solidified with kudzu root starch, it was observed that between 8 and 24 hour incubation. 10 and 12% solids stimulated greater cell production than did 6 and 8% solids. The concentration of solids also affected thd secretion of protein from the yeast cells with the highest content of extracellular protein at 10-24 hour incubation stimulated by 10% starch solids.
Characteristics of Pseudomonas sp. degrading 2-methyl-4-chlorophenoxyacetic acid
The Korean Journal of Microbiology, volume 24, issue 4, 1986, Pages 389~393
From the soil and river samples, some bacterial strains degrading chlorinated aromatic hydrocarbons were isolated and identified. Of the isolates, seven strains of Pseudomonas sp. harbouring plasmids were selected for their prominent degradative ability to 2-methyl-4-chlorophenoxyacetic acid. By agarose gel electrophoresis and curing experiment it was found that the genes for 2-methyl-4-chlorophenoxyacetic acid degradaiton were encoded on the plasmids in these selected strains. Antobiotic resistance and degradative ability for other herbicides of the strains were tested.
Characteristics of MCPA plasmid isolated from pseudomonas sp.
The Korean Journal of Microbiology, volume 24, issue 4, 1986, Pages 394~399
From the lysates of the 7 selected strains of Pseulomonas utilizing 2-methyl-4-chlorophenoxyactate as a sole source of carbon and energy, several MCPA plasmids, which encodes genes for the degradation of 2-methyl-4-chlorophenoxyacetate, were isolated, and measured their molecular weight as well as genetic characters such as resistance to antibiotics and degradative ability of other chlorinated herbicides. Transmissibility of the MCPA plasmids, pKU1, pKU15, and pKU17 was tested by conjugation or transformation and the restriction pattern of pKU15 for Pvu II, Hind III, EcoR I, Xho I, Bgl II, and Ava II was analyzed.
Construction of a Phylogenetic Tree from tRNA Sequences
The Korean Journal of Microbiology, volume 24, issue 4, 1986, Pages 400~405
We have constructed a phylogenetic tree for eleven species by comparing their tRNA sequences. The tree suggests that prokaryotes diverged very early before the emergence of animals. The fact that H. volcano, an archaebacterium, clusters with eukaryotes implied that eukaryotes did not diverge directly from thier common ancestor with eubacteria. The branching order of phage
indicates that they have diverged separately from thier hosts and they might have evolved independently. A correlation between nucleotide substitution in tRNAs and paleontological record was observed. We verified that our phylogenetic tree fits very well with traditional ones very well by imposing the molecular clock on the tree.
A new restriction endonuclease from xanthomonas citri
The Korean Journal of Microbiology, volume 24, issue 4, 1986, Pages 406~410
The isolation and charateriation of a type II restriction endonuclease from Xanthomonas citri IFO 3835 were described. This enzyme (Xci I endonuclease) is an isoschizomer of Sal I endonuclease recognizing 5'-GTCGAC-3' and cleaving at the site indicated by the arrow. Unlike Sal I endonuclease, Xci I endonuclease requries a NaCl concentration of 50mM for its maximum activity.