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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 25, Issue 4 - Dec 1987
Volume 25, Issue 3 - Sep 1987
Volume 25, Issue 2 - Jun 1987
Volume 25, Issue 1 - Mar 1987
Selecting the target year
Properties of an R plasmid pKU41 from pseudomonas putida KU190
The Korean Journal of Microbiology, volume 25, issue 2, 1987, Pages 87~93
The properties of the plasmid pKU41 isolated from Pseudomonas putkda KU190 have been investigated, pKU41 was defined as an R plasmid having a transmissible ampicillin and tetracycline resistance determinant, and could be classified as a plasmid belonging to IncP-1 group according to incompatibility grouping.
Characterization of the Folding Structure of 3'-end of Lactate Dehydrogenase A-mRNA Isolated from Hormone Stimulated Rat
Glioma cell culture
The Korean Journal of Microbiology, volume 25, issue 2, 1987, Pages 94~102
Rat liver LDH A-cDNA has been isolated from a .lambda.gt11-rat lover cDNA library and partially characterized. The size of the isolated rat liver LDH A-cDNA if shown to be 1.6Kb and restriction enzyme sites for the rat liver LDH A-cDNA are also mapped. 682-nucleotide sequence coding for 3'-end of rat liver LDH A-cDNA has been analyzed and compared to the nucleotide sequence of the same region of rat
-glioma cell LDH A-cDNA which has been cloned from the hormonally stimulated cell cultures. The result shows that 177 nucleotide sequences coding for the C-terminal 59-amino acids are identical but 505 nucleotide sequences of 3'-nontranslated region of the two LSH A-cDNA exhibit characteristic differences in thier nucleotide sequences. Computer analysis for the folding structures for 3'-end 400 nucleotide sequences of the two LDH A-cDNA shows a possibility implying that the two LDH A-mRNAs isolated from different tissues of rats may have different half life and therefore their translational efficiency may be different. It has been previously demonstrated that isoproterenol stimulated rat
-glioma cell cultures produce LDH A-mRNA showing 2 to 3-fold longer half life in comparison to that of noninduced LHD A-mRNA. The result therefore support for the idea that hormonally stimulated rat
-glioma cells may produce LDH A-mRNA containing different nucleotide sequences at the 3'-end nontranslated region by which the hormonally induced LDH A-mRNA could have more stable secondary mRAN structure in comparison to that of noninduced LDH A-mRNA.
A gene responsible for ozone sensitivity (ozrB) in chromosome of escherichia coli B. MQ 1844
The Korean Journal of Microbiology, volume 25, issue 2, 1987, Pages 103~109
An ozone-sensitive mutant of Escherichia coli strain B, MQ 1844 is described. Its properties, including high sensitivity to ozone and radiation, inducible filamentation, extensive DNA degradation and impaired DNA synthesis following ozonation, are attributable to a mutation in ozrB, a gene which is cotransducible with malB. Based on differences in phenotypic expression as well as on the particular location of this gene on the bacterial chromosome, ozrB appears as distinct from the other ozone-or radiation-sensitivity genes previously described.
Effect of Triton X-100 on Intracellular Accumulation of Cadmium in Hansenula anomala
The Korean Journal of Microbiology, volume 25, issue 2, 1987, Pages 110~116
As a pary of investgation on effective accumulation of cadmium in yeast cells, effect of surfactant was studied on intracellular accumulation of cadmium in extremely cadmium-tolerant yeast, Hansenula anomala B-7. Cadmium accumulation was enhanced up to approximately 40% by the addition of non-ionic surfactant, Triton X-100 and its optimal concentration was found to be 0.1-0.2%. Furthermore, optimum conditions for intracellular accumulation of cadmium were at
and initial pH 6.0 for 48 hours under shaking culture.
Induced reactivation of T3 phage in ozone treated strains of Escherichia coli B
LHerault Pierre ;
The Korean Journal of Microbiology, volume 25, issue 2, 1987, Pages 117~121
The ozone-induced reactivation factor for ozonated or UV-irradiated T3 phage was determined in defferent bacterial strains of Escherichia coli B resistant of sensitive to ozone. Our results suggest that ozone is a weak, if any at all, inducer of the Weigle reactivation, one of the SOS functions. This is in agreement with other studies which have suggested that thes agent is probably a weak inducer of the SOS functions.
Isolation and characterization of bacteria degrading chlorinated aromatic hydrocarbons
The Korean Journal of Microbiology, volume 25, issue 2, 1987, Pages 122~128
Several bacterial isolates capable of degrading 4-chlorobiphenyl or 2,4,5-trichlorophenoxyacetic acid were isolated from industrial wastes by the agar plate method and studied for their biodegradabilities of the hydrocarbons and some biochemical characteristics. The isolates DJ-12, DJ-26 and TP-1 were identified as Pseudomonas spp. and they could not degrade 2,4-dichlorophenoxyacetic acid. The absorption spectra for 4-chlorobiphenyl and 2,4,5-trichlorophenoxyacetic acid showed the peaks at 253 and 292 nm, respectively. Biodegradability of the isolates was determined by decrease of the absorbance for the test hydrocarbons with a UV-scanning spectrophotometer. The plasmids of the isolates were studied to examine whether or not the hydrocarbon-degrading genes exist in the plasmids. Antibiotics resistance was also examined to search out a proper marker for the isolates in further experiments, such as curing test and genetic recombination.
Characterization of Azospirillum spp. Isolated from Korean Paddy Roots
The Korean Journal of Microbiology, volume 25, issue 2, 1987, Pages 129~136
Nitrogen fixing activity associated with 40 varieties of rice was assayed at heading stage by an in situ acetylene reduction method. The in situ acetylene reduction activity and population of nitrogen fixing bacteria obtained on nitrogen-free malate medium for Azospirillum spp. enrichment showed positive correlation. Six Azospirillum spp. with high nitrogenase activity were isolated from the rice roots, from which five spp. were identified as A. lipoferum and one was A. brasilense. The physiological characteristics of the six Azospirillum isolates, that is, carbon source utilization, biotin requirement, antibiotic resistance, indole acetic acid excretion, plasmid profile and protein patterns were compared.
Environmental factors affecting seasonal destribution of heterotrophic bacteria in Kum river estuarine sediments
The Korean Journal of Microbiology, volume 25, issue 2, 1987, Pages 137~143
Dominant genera of heterotrophic bacteria were investigated seasonally in Kum River estuarine sediments. Numerical taxonomy on the isolated heterotrophic bacteria were carried out according to their morphological, physiological, nutritional and biochemical characters. Heterotrophic bacteria in the depths of 0 to 10 cm of sampled sediments were almost facultative anaerobes, and 77.9% to 86.9% of them were Gram negative rods. Kominant genera among the isolates were Pseudomonas, Flavobacterium, Micrococcus, Cnterobacteria and Vibrio during sampling periods. According to their character analyses by similarity index(S), the isolates were clustered in fourteen groups at clay type sediments (Site 1) and four groups at sand type sediments (Site 2).
Photosynthetic membranes of Rhodocyclus gelatinosus and Rhodocydlus tenuis
The Korean Journal of Microbiology, volume 25, issue 2, 1987, Pages 144~147
Intracytoplasmic photosynthetic membranes of Rhodocyclus gelatinosus and Rhodocyclus tenuis are known to be of weakly developed type of in the Rhodospirillaceae. We compared the membrane invagination of Rhodocyclus gelatinosus KS-117 (isolated in lur laboratory) with that of Rhodocyclus tenuid (ATCC 25093) after culturing under several different light intensities. We observed the significant membrane invagination in Rhodocyclus gelatinosus, in particular under 3,000 Lux, while none was observed in Rhodocyclus tenuis.
Characterization of laccase from pleurotus ostreatus
The Korean Journal of Microbiology, volume 25, issue 2, 1987, Pages 148~156
Extracellular laccase (E.C. 126.96.36.199) from the culture filtrate of Pleurotus ostreatus was purified by ammonium sulfate precipctation, protamine sulfate precipitation, DEAE-Sephadex A-50 ion exchange chromatography and Sephadex G-100 gel permeation chromatography. The molecular weight of the enzyme was estimated by SDS-polyacrylamide gel electrophoresis to be 58,000 and the isoelectric point was 3.75. The optimum temperature for the enzyme was about
and the optimum pH was 6.5. The enzyme was found to be stable at temperature below
and rapidly inactivated at higher temperatures. Km values for ferulic acid, vanillic acid, dihydroxyphenylalanine (DOPA) were 48.6.
M, 0.52mM, and 2.73mM, respectively, which indicates that the enzyme has much higher affinity towards ferulic acid. The reaction products of the enzyme were separated by TLC and HPLC.
Purification and Properties of Endo-
-1, 4-glucanase from Thermophilic Clostridium thermocellum
The Korean Journal of Microbiology, volume 25, issue 2, 1987, Pages 157~164
A new endo-
-1, 4-glucanase was purified from the culture filtrate of thermophilic anaerobic Clostridium thermocellum. The purification procedure included two steps of ion exchange chromatography with DEAD-Sephadex A-50 and gel filtration chromatography with Sephadex G-75. Even though the 56 fold increase in CMCase specific activity was obtained, the actually recovered enzyme activity was relatively lower level of 0.7%. Judging from the two bands in SDS-polyacrylamide gel electrophoresis, the endo-
-1, 4-glucanase consists of two subunits whose M.W. are 38,000 and 58,000, respectively. The optimum pH and temperature were determined to be 5.0 and
, respectively. The enzyme was stable up to
, but inactivated at
. The kinetic parameters of the separated fraction were also determined. The purified enzyme did not show any significant hydrolytic activity against the highly ordered crystalline cellulose as well as filter paper.