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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 25, Issue 4 - Dec 1987
Volume 25, Issue 3 - Sep 1987
Volume 25, Issue 2 - Jun 1987
Volume 25, Issue 1 - Mar 1987
Selecting the target year
Construction of an expression vector with SV40 DNA in a mammalian cell
The Korean Journal of Microbiology, volume 25, issue 3, 1987, Pages 165~172
An expression vector in a mammalian cell was constructed using the origin of replication (OR) and the promoters of SV40. The plasmid pSVOE was constructed by inserting SV40 DNA fragment (1, 118bp) containing SV40 OR and promoters into pBR322-1, and then a multiple cloning sequence was inserted at the immediate downstream of the late promoter of SV40 in the pSVOE vector. The plasmid was named pSVML. As a selection marker, thymidine kinase gene of herpes simplex virus with its promoter was inserted into EcoRI site of pSVML and the recombinant was named pSVML-TKp. To test the expression capacity of foreigen gene inserted at the multiple cloning site of pSVML, the thymidine kinase gene without its own promoter was inserted at the BamHI site of pSVML. The recombinant was named pSVML-TK. These plasmids, pSVML-TKp and pSVML-TK, were transfected into COS cells with calcium phosphate precipitation method. The thymidine kinase activity was significantly increased in both transfected cells.
Restriction endonuclease mapping of the plasmid pTi12 from agrobacterium tumefaciens
The Korean Journal of Microbiology, volume 25, issue 3, 1987, Pages 173~179
Ti plasmids were isolated from three strains of Agrobacterium tumefaciens in Korea and their types and molecular weights were determined. All of these are octopine-type and their molecular weights are 44Kb (pTi 12), 180Kb (pTi 14) and 172Kb (pti 49), respectively. In order to construct physical map of pTi 12, pTi 12 was digested with restriction endonucleases Sma I and Hind III. Sma I degestion of pTi 12 produce 8 fragments and Hind III produced 10 fragments. Physical arrangements of these fragments was determined by Southern hybridization techniques.
Purification and Characterization of stu I Endomuclease from Streptomyces Tubercidicus
The Korean Journal of Microbiology, volume 25, issue 3, 1987, Pages 180~183
Stu I, type II restriction endonuclease, has been purified to homogeneity from Streptomyces tubercidicus (ATCC 25502), and its catalytic properties have been studied. For the purification of Stu I endonuclease free of nonspecific nucleases, DEAE-Sephadex (A-50), QAE-Sephadex (A-50) and Heparin-agarose column chromatography have been performed after ammonium sulfate fractionation of the crude extract. The enzyme was further purified by gel filtration using Sephadex G-100 column to obtain homogeneous form of protein. The single polypeptide species of Stu I endonuclease has a subunit molecular weight of 34,000
1,000 daltons as judged by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Stu I endonuclease requires
ion for its activity and is maximally active at neutral pH (7.0-8.0) in the absence of NaCl.
Ingibition of coliphage N4 infection to escherichia coli mutant defective in mannose permease
The Korean Journal of Microbiology, volume 25, issue 3, 1987, Pages 184~188
Evidences that the mannose permease of Escherichia coli mediates the infection of N4 in early steps, were obtained as follows. First, A mutant strain of Escherichia coli which was resistant to both wild type N4 and lambda whose genome is Charon 4A containing human genomic fragments in its EcoR I site, could not use mannose efficiently. Second, N4 could not infect pel mutant strains which lack one or all of intact components of mannose permease. However, unknown alterations in N4 made it possible for the phage to infect pel mutant of E. coli. It also turned out to be clear that the receptor of N4 was different from that of lambda.
Regulation of Cell Growth and Tylosin Biosynthesis through Flux Control of Metabolic Intermediate in Streptomyces fradiae
The Korean Journal of Microbiology, volume 25, issue 3, 1987, Pages 189~197
The aim of the present study was to investigate the effect of glutamate on the biosynthesis of tylosin. Activities of enzymes involved in the metabolic pathway of glutamate to form tylactone, an essential precursor of tylosin, were determined using Streptomyces fradiae grown at different concentration of glutamate. As results, it was found that cell growth and tylactone formation was controlled by the metabolic flux of oxaloacetate. It was clear that cell growth was favored by the activities of citrate synthase and aspartate aminotransferase, while the tylactone synthesis was stimulated by the activity of methylmalonyl-CoA carboxyltransferase. Therefore it was concluded that channelling of oxaloacetate was a point for favoring either cell growth or tylosin biosynthesis.
Properties of biparental clones formed by spheroplast fusion of pseudomonas putida
The Korean Journal of Microbiology, volume 25, issue 3, 1987, Pages 198~204
Biparental clones and recombinant clones were obtained by spheroplast fusion of Pseudomonas putida KU218R-3 and P.putida KU428. Formation of the fusion product was the most effective when the Pseudomonas spheroplast mixture were treated with 40% plyethyleneglycol(PEG) 6000 for 10min at room temperature, The fusants which selected by indirect method were obtained at an average frequency of 10.8%. Most of the fusants were biparental clones (10.4%) and the recombinant clones were produced in low yield (0.42%). Fusants, at the frequency of 4% were obtained without PEG 6000, which shows that fusion is not strictly dependant on PEG. The stability of fusants were examined. Most of the biparental clones were segregated to parental form amd late recombinants were formed on further propagation of biparental clone but the recombinant clones were nery stable.
Characterization of methionine analogue-resistant mutant of cephalosporium acremonium
The Korean Journal of Microbiology, volume 25, issue 3, 1987, Pages 205~211
Cephalosporium acremonium MAR-80, a strain of methionine analogue-resistant mutant, showed good activity of sulfate utilization as only sulfur source. The effect of methionine on the sulfate uptake system was investigated by using
as a tracer in the resting cell system. From this result, it was revealed that sulfate permease of this strain was less repressed and/or less inhibited by methionine than parent type. This deregulation was due to low actibity of methionine uptake, which was operated by somewhat simple diffusion. From these studies, it could by anticipated that the improved productivity of cephalosporin C and lower dependence of cephalosporin C production on methionine were related to increased uptake rate of sulfate.
Isolation of an Actinomycetes Producing Extracellular Adenine Deaminase and Cultural Conditions of the Isolated Strain for the Enzyme Production
The Korean Journal of Microbiology, volume 25, issue 3, 1987, Pages 212~220
The taxonomical properties of strain J-275L isolated from soil as a microorganism which produces extracellular adenine deaminase and cultural conditions for the enxyme production were studied. The hyphae of strain J-275L is fragmented into rod-or coccus-like elements. The elements of fragmented aerkal hyphae has smooth surfaces. The cell wall of the organism contains LL-diaminopimelic acid. Mycolic acid are not produced. As a result of taxonomical studies, strain J-275L is designated as Nocardioides sp. J-275L. The optimum medium for the enzyme production from Nocardioides sp.J-275L wascomposed of 0.5% peptone, 0.5% dextrin, 1% yeast extract, and 0.2%
. The optimum initial pH of the medium was pH 7.5.
Purification and Properties of Extracellular Adenine Deaminase from Nocardioides sp. J-257L
The Korean Journal of Microbiology, volume 25, issue 3, 1987, Pages 221~228
The extracellular adenine deaminase from Nocardioides sp. J-275L was purified by the following techniques: ammonium sulfate fractionation, DEAE-Cellulose, DEAE-Sephadex A-50 column chromatography, and Sephacryl S-200 superfine gel filtration. The enzyme was partially purified about 3889.5-fold with about 5.2% yield by these procedures. The molecular weight of the enzyme was 39,000 by a calibrated Sephacryl S-200 superfine column chromatography. The enzyme was stable at pH 7.5 and up to
. Glycerol was effective on the stabilization of the enzyme during storage. The optimum pH and temperature of the enzyme were around pH 7.5 and
, respectively. The apparent Michaelis constant Km of the enzyme for adenine was
M. The purine analogues, 6-chloropurine, 2,6-diaminopurine, 6-bromopurine, 4-aminopyrazolo [3.4-d]pyrimidine, and 8-azaadenine were substrates for the enzyme. 6-Dimethylaminopurine was a competitive inhibitor of the enzyme. The enzyme was inhibited by 1mM of
, and 1mM of
'-dipyridyl, pentachlorophenol, and pCMB.
Variations of diversity and tolerance indicies of heterotrophic bacterial communities in Naktong estuary
The Korean Journal of Microbiology, volume 25, issue 3, 1987, Pages 229~237
To determine the characteristics of heterotrophic bacterial community in estuarine ecosystem, water and sediment samples were taden from Naktong estuary. All isolates were compared with 73 characters and described by cluster analysis. With same characters, 30 reference strains were able to divide into approximate species level at 80% similarity (S value). Diversity indices (
) of sediment column isolates were higher than water column isolates. The bacterial community commonly appeared in water and sediment column was reduced with going to downstream. Tolerance indices for temperature (Pt) and salinity (Ps) were also higher in sediment isolates than in water isolates. The bacterial community in sediment column is believed to be composed with diverse populations compared to water column and maintains its stability against various environmental changes with high physiological tolerances.
Isolation and Identification of an acidoduric Streptomyces sp. from Forest Soil
The Korean Journal of Microbiology, volume 25, issue 3, 1987, Pages 238~243
In this study, an acidoduric Streptomyces strain was isolated and identified from acidic forest soil around Dankook University, Cheonan Campus. This isolated strain had rod-shaped, smooth, non-motile spore and the shape of spore chain was compact spiral. This structure appeared similar to the sporangium of the genus Streptosporangium but this strain proved to be a Streptomyces strain by an electron microscopic study and cell wall analysis. This strain showed a best growth on neutral medium, was also able to grow on the acidic media of pH 4.0 and pH 5.0. The color determination of this strain on various agar media and other physiological tests were carried out by ISP-methods. From these results, the isolated strain was considered to be Streptomyces mirabilis.
Differentiation of mixed bacterial populations by modified gram stain
The Korean Journal of Microbiology, volume 25, issue 3, 1987, Pages 244~248
Attempts were made to enumerate the number of Gram positive and negative bacteria in the development of natural fermentation rapidly and simultaneously. A general Gram stain was applied to this study. The number of cells by Gram stain was proportional to the cell turbidity by spectrophotometer within a range of 0.7 absorbance at 610nm. The cells washed out during procedures were not exceeded about 8 percentage. The standard error of separate counts in the mixture of Cscherichia coli and Micrococcus luteus was
%. The possible range of counting was
cells/ml. Therefore, it is believed that a general Gram stain could be applied to the separate counting of mixture of Fram positive and negative bacterial populations too. In practice, growth kinetics of hemp retting and Kimchi fermentation were presented.
Growth inhibition of Saccharomyces cerevisiae by alternation current pulse
The Korean Journal of Microbiology, volume 25, issue 3, 1987, Pages 249~253
Effects of Ac pulse at low voltage on Saccharomyces cerevisiae were studied. The treatment of yeast suspensions contained 0.2m NaCl with 500mA for 350 sec at
was shown about 50% of lethality, whereas in the treatment of the same suspensions with 250mA for 250 sec at the temperature (
) corresponding to about 10% of lethality, growth was completely inhibited instead of lethality. The effect of growth inhibition was due to occurrence of auxotrophic strains under experimental conditions. Detection of auxotrophic yeasts was done tentatively with the difference of the number of viable yeast cells between direct counting by methylene blue staining and plate-count on yeast morphology agar.