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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 26, Issue 4 - Dec 1988
Volume 26, Issue 3 - Sep 1988
Volume 26, Issue 2 - Jun 1988
Volume 26, Issue 1 - Mar 1988
Selecting the target year
Characterization of BmaI endonuclease from bacillus macerans ATCC 8244
The Korean Journal of Microbiology, volume 26, issue 1, 1988, Pages 1~5
The esolation and characterization of a new type II restriction endounclease, BamI, from Bacellus macerans ATCC 8244 were described. BmaI endonuclease was partially purified by procedures of ammonium sulfate fractionation, DEAE-cellulose and phosphocellulose chromatographies. This enzume recognized one site on pBR322 DNA, two sites on Bluescribe DNA, three sites on
DNA and no site on SV 40 DNA. The same cleavage patterns for vareius DNAs as PvuI indicated that BamI is an isoschisomer of PvuI whose recognition sequence is 5'-CGATCG-3'. The optimal pH for the BmaI endonuclease activity was about 7.0 and optimal NaCl concentration was about 100mM. Manganese ion could partially replace magnesium as a cofactor, but calcium could not at all.
Exprission of cellulomonas biazotea cellobiase gene in E. coli
The Korean Journal of Microbiology, volume 26, issue 1, 1988, Pages 6~12
-glucosidase) is an enzyme of the cellulase system in cellulolytic microor-ganisms. The chromosomal DNA fragment which include cellobiase gene of Cellulomonas biazotea was cloned in Eschericia coli via plasmid pBR 322 vector. Restriction enzyme Sal I was used to obtain adequate size of fragments from C. biazotea. chromosomal DNA. The transformant of E. coli HB101 with recombinant plasmid pBG101 showed cellobiase activity, which is not ordinary in E. coli HB101. The enzyme activity of the transformant was as of 20% lower than that of C. biazotea.
Characterization of R plasmid pKU 41 from pseudomonas putida KU190
The Korean Journal of Microbiology, volume 26, issue 1, 1988, Pages 13~19
The location of R-determinants,
, and replication origin in pKU41 determined using the construction of miniplasmid by the BamHI and the HindIII restriction fragment from pKU41 and the cloning of the restriction fragments from pKU41 into pSY343. The gene encoding resistance to ampicillin (Ap) as well as replication origin in pKU41 were located on the region overlapping BamHI B fragment and HindIII A fragment. The gene encoding resistance to tetracycline (Tc) was located on the region of the HindIII C fragment, which was cleaved by BamHI as well.
Isolation of Nif
-mutants through transposon mutagenesis in enterobacter agglomerans 339
The Korean Journal of Microbiology, volume 26, issue 1, 1988, Pages 20~26
-mutants were isolated from Enterbacter agglomerans 339 through the transposon umtagenesis using a RP4-mobilising system for its nif-gene characterization. All mutants hadn't acetylene-reduction ability. Then we confirmed that Tn5 was inserted into all conserved nif-plasmids through the Southern Hybridization.
Biosynthesis of messenger RNA in aspergillus phoenicis during thier life cycle
The Korean Journal of Microbiology, volume 26, issue 1, 1988, Pages 27~31
Biosynthesis and processing of cytoplasmic mRNA from heterogenous nuclear RNA (hn-RNA) in Aspergillus phoenicis were studied by
-uridine labeling and synchronous culture techniques during their life cycle. Incorporations of
-uridine into hn-RNA and mRNA were most rapid in vesicle-phialide fromation stage and diminished in hyphal growth stage. The processing of cytoplasmic mRNA from hn-RNA was proceeded more rapidly in hyphal growth and conidiophore formation stages than in conidia and vesicle-phialide formation stages. The specific radioactivities of hn-RNA and mRNA were very high in vesicle-phialide formation stage.
Molecular cloning and restriction analysis of aspartokinase gene (HOM3) in the yeast, saccharomyces cerevisiae
The Korean Journal of Microbiology, volume 26, issue 1, 1988, Pages 32~36
The yeast gene HOM3 encodes aspartokinase, which catalyses the first step (aspartate to and from beta-aspartyl phosphate) of common pathway to threonine and methionine. The yeast HOM3 gene expression is known to be regulated by threonine and methionine specific control, and also by general control of amino acid biosynthesis. Isolation and characterization of the HOM3 gene are essential for the molecular genetic study on its regulation of expression. A recombinant plasmid pSC3 (15.5kb, vector YCp50) has been cloned into E. coli HB101 from yeast genomic library through their complementing activity of HOM3 mutation in a yeast recipient strain M34-24B. Organization of the plasmid was characterized by delineation of restriction cleavage sites in the insert fragment.
Protoplast fusion between saccharomyces cerevisiae and candida cariosilignicola
The Korean Journal of Microbiology, volume 26, issue 1, 1988, Pages 37~43
This research was focused on investigation of the condition for protoplast formation and regeneration of protoplast fusion between Saccharomyces cerevisiae which has fermentation ability and Candida cariosilignicola which can grow at high temperature and utilize methanol. The results obtained were as follows; The highest production was collected in exponential growth phase. Ninety-nine% protoplast formation of C. cariosilignicola was obtained in glycin-NaOH buffer (pH10.0) containing Zymolyase 0.5mg/ml at
for 1hr incubation. The highest regeneration was produced when protoplast wuwpension containing 0.5% soft agar in buffered 50mM
was poured as a soft overlay onto 2% agar plates. Equal amuont of protoplast suspension of two strains was mixed and centrifuged. The subsequent pellet was added to 2ml of 35% polyethylene glycol (MW 4,000) containing 50mM
, and incubated at
for 10min. Then 0.1ml of the suspension of aggregated protoplast was immediately covered with minimal medium and incubated at
for 5-7 days. As results,
fusants were obtained. The physiological characteristics of fusants produced by protoplast fusion were;
utilized maltose, galactose, methanol, potassium nitrate.
utilized all the above materials except galactose.
Studies on the cell cycle of saccharomyces cerevisiae by electron spin resonance spectroscopy
The Korean Journal of Microbiology, volume 26, issue 1, 1988, Pages 44~51
The intracellular free radicals produced at different stages of cell cycle of Saccharomyces cerevisiae ATCC 24858 were investigated by means of electron spin resonance(ESR) spectroscopy. The synchronized cells by repeated starvation and refeeding revealed different ESR spectral pattern compared to that of asynchronized cells. Each spectrum centered at g=2.005, which indicates free radicals. The relative spin concentration was maximat at the end of DNA increase. The variation of the relative spin concentration at each distinct stage of the cell cycle was evaluated in relation to ascorbate concentration, L-galactonolactone oxidase activity, and ascorbate oxidase activity. The highest activities of L-galactonolactone oxidase and ascorbate oxidase were detected just before and at the maximum of relative spin concentration, respectively. And ascorbate concentration fluctuated through each stage of cell cycle with the changes of relative spin concentration, L-galactonolactone oxidase activity, and ascorbate oxidase activity. Thus it is suggested that intracellular free radicals should be related to cell cycle, interacted with ascorbate, and may play an important role in the cell cycle of Saccharomyces cerevisiae.
Characterization of L-Galactono-1, 4-lactone Oxidase Purified from Saccharomyces cerevisiae
The Korean Journal of Microbiology, volume 26, issue 1, 1988, Pages 52~59
A partially purified preparation of L-galactonolactone oxidase which catalyzes the last step of L-ascorbic acid biosynthesis was obtained from Saccharomyces cerevisiae ATCc 26787. The purification procedures included Triton X-100 treatment, protamine sulfate precipitation, ammonium sulfate precipitation, DEAE-Sepharose CL-6B ion exchange chromatography, Sephadex G-150 gel filtration chromatography, and Phenyl-Sepharose CL-4B hydrophobic interaction chromatography. The optimum temperature for the enzyme activity was about
and the optimum pH was 6.8-7.0. The substrate specificity was confined to L-aldonolactones, L-galactono-1,4-lactone and L-gulono-1,4-lactone. An apparent Km value of 0.294mM with L-galactono-1,4-lactone as a substrate was found. By comparing the substrate specificities of this enzyme with those of isofunctional enzymes of higher plants and animals, it becomes evident that the enzyme of S. cerevisiae ATCC 26787 is rather similar to the L-gulonolactone oxidase of animals than the galactonolactone dehydrogenase of higher plants.
Colonization of gram-negative bacterial community in aerobic hemp retting
The Korean Journal of Microbiology, volume 26, issue 1, 1988, Pages 60~66
Dynamics of bacterial communities and its colonization under aerobic gemp retting were observed in air lift fermentor as a closed system, unlike conventional hemp retting as an open system. Dried hemp which was harvested in both 1986 and 1987 was retted at room temperature. Predominant community was facultatively anaerobic Gram-negative rods, and its density was increased from
cells/ml. The density of facultatively nanerobic Gram-positive fods was maintained at the lovel of
cells/m, and this Gram-positive bacterial community was not participated in retting. In the Gram-negative bacterial community during the retting, five types of colonieswere developed at early stage of pH7.0-8.0, and thereafter, only three types were colonized till later stage, shich were identified as pectolytic strain Erwinia salicis, Erwinia tracheiphila and Enterobacter agglomerans. A community of facultatively Gram-negative rods was mainly proliferated in stems and dispersed into liquor after 6-8 hours. Retting was terminated within 70-80 hours.
The effect of light on baker's yeast cell growth and protein secretion
;;L.A.Hojnicki;Malaney, G.W.;Tanner, R.D.;
The Korean Journal of Microbiology, volume 26, issue 1, 1988, Pages 67~71
It has been observed that white loght can suppress both cell growth and protein secretion in Baker's yeast. This effect was explored in batch liquid fermentations. Possible applications of this phenomenon are (a) use as a tool for pre-concentrating excreted enzymes prior to subsequent purification and (b) an engineering variable for regulation yeast fermentations.