Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 26, Issue 4 - Dec 1988
Volume 26, Issue 3 - Sep 1988
Volume 26, Issue 2 - Jun 1988
Volume 26, Issue 1 - Mar 1988
Selecting the target year
Detection of Japanese Encephalitis Virus by Biotinylated cDNA Probe
The Korean Journal of Microbiology, volume 26, issue 3, 1988, Pages 149~154
Japanese Encephalitis Virus(JEV) can be detected conveniently by the use of biotinylated cDNA probe. To prepare biotinylated probe aminoallyl dUTP was first synthesized chemically to reverse transcribe the virial RNA. The allylamine-labeled cDNA was then converted to the biotin-cDNA by the reaction with an activated biotin ester, NHS-ACA-biotin. The JEV genomic RNA was hybridized to the biotinylated cDNA probe on nitrocellulose filter and visualized colorimetrically by streptavidin complexes with alkaline phosphatase polymer. Sensitivity of the detection system was determined by estimating the amount of the JEV genomic RNA through comparison with signals generated from the biotinylated and
-labeled probes. It was found that the biotin probe was as sensitive as
-cDNA probe which can detect 50pgs of the target RNA.
Nucleotide Sequence Homology in Rotaviruses
;Spendlove, Rex S.;Barnett, Bill B;
The Korean Journal of Microbiology, volume 26, issue 3, 1988, Pages 155~161
Nucleotide sequence homology between bovine, simian, and porcine rotavirus was determined by the RNA:RNA hybridization technique. Single stranded RNA, prepared in vitro with EDTA activated endogeneous viral RNA polymerase, was hhbridized with tritium labeled bovine rotavirus genomic RNA. The heteroduplex RNA was treated with single stranded RNA specific ribonucleases and the RNase resistant hybrid RNA was precipitated, and collected by filtration on a filter paper. Seventy four percent RNA sequence homology between bovine and simian rotavirus and 8 percent RNA sequence homology between bovine and porcine rotavirus was confirmed by hybridization between tritium labeled single stranded RNA and viral genomic RNA.
Inhibition of SV40 DNA replication in simian cell by bacteriophage M13 DNA sequences
The Korean Journal of Microbiology, volume 26, issue 3, 1988, Pages 162~166
Bacteriophage M13 DNAs carrying the wild type or base substituted SV40 DNA replication origins were used for replication assay. In vivo and in vitro assay with African green monkey cell line COS-1 showed that the replication of M13-SV40 recombinant DNAs was restricted like a pBR322 SV40 recombinant DNA(Lusky and Botchan, 1981). Furthermore, recombinant phage DNAs isolated from the transfected siminan cells subsequently show a reduced ability to retransform E. coli. But pATSV-W(Kim et al., 1988) was replicated in COS-1 cells normally. We think that a poison sequence may exist on bacteriophage M13 DNA like pBR322.
A genetic map of the R-factor pKU10 isolated from pseudomonas putida
The Korean Journal of Microbiology, volume 26, issue 3, 1988, Pages 167~172
A genetic map of the IncP-1 group plasmid pKU10 has been prepared through the construction of recombinant plasmids containing various fragments of pKU10. Phenotypic analysis of these derivatives has identified the location of genes encoding resistance to ampicillin, tetracyclin, and chloramphenicol. The region involved in conferring resistance to ampicillin was located around two PstI sites that are 1.0Kb apart. The tetracyclin resistance gene was mapped on the region of HindIII E fragment and a part of HindIII D fragment, and the determinant for chloramphenicol resistance gene was localized on HindIII D fragment.
Transfer RP4::Mu cts and RP4::mini-Mu from E. coli to Pseudomonas sp.
The Korean Journal of Microbiology, volume 26, issue 3, 1988, Pages 173~180
Chromosomal gene transferable hybrid plasmids, RP4::Mu cts and RP4::mini-Mu, were transferred by conjugation from E. coli to Pseudomonas strains. In order to use for recipient cells of RP4::Mu cts and RP4:: mini-Mu, plasmid-free Pseudomonas strains were characterized for their antobiotic resistance, aromatic hydrocarbon utility and degradation patterns of chlorinated herbicide. Transfer frequencies of RP4::mini-Mu exhibited about
, while those of RP4::Mu cts exhibited very low value of
in recipients tested except Pseudomonas aeruginosa KU557. Existance of hybrid plasmids in Pseudomonas transconjugants were identified by their antibiotic resistance and agarose gel electrophoresis. In case of RP4::Mu cts transconjugants it was also confirmed by demonstrating that they were capable of releasing phage and forming plaques at
. Plaque forming unit of the transconjugants was about
. It was shown by the stability test that RP4::Mu cts and RP4::mini-Mu in Pseudomonas were relatively stable.
Transfer properties of nif-plasmid pEA 9 by pEA 9::Tn5-Mob
The Korean Journal of Microbiology, volume 26, issue 3, 1988, Pages 181~187
Using a Tn5-Mob system, pEA9 was characterized as a self-transmissible plasmid carrying a kanamycin resistance marker. The self-transfer frequencies of pEA9 varied greatly depending on pH values. The transfer frequency was about
at pH 5, that was 10 times higher than one at pH 6.5. With a helper plasmid, transfer frequencies were increased about
times than the frequencies obtained without it.
Protoplast Fusion of Lactose Assimilating Yeasts
The Korean Journal of Microbiology, volume 26, issue 3, 1988, Pages 188~196
Intergeneric or intraspecific protoplast fusion between Saccharomyces cerevisiae X-2180-1A, Candida pseudotropicalis ATCC 8619 and CBS 607 was attempted to produce ethanol from lactose containing materials. Teh intergeneric fusion frequency between Saccharomyces cerevisiae X-2180-1A (ade rho) and Candida pseudotropicalis CBS 607 (his met) was
. These values exhibited approximately 2-3.5 fold increase when compared with fusion frequency obtained without the treatment of bovine serum albumin, myoinositol and ergosterol, suggesting that these compounds may improve intergeneric of intraspecific protoplast fusion. Nuclear fusion appears to occur in fusants between intergenera(S. cerevisiae+C. pseudotropicalis) and intraspecies (C. pseudotropicalis strains) as strongly suggested by DNA content, nuclear staining, comparison of survival rate to UV light and isolation of recombinants after mitotic segragation. It was also found that alcohol production from intraspecific hybrids was somewhat increased when compared with that from their parents.
Characterization of the Outer Membrane-Associated 2-Furaldehyde Dehydrogenase from Klebsiella pneumoniae
The Korean Journal of Microbiology, volume 26, issue 3, 1988, Pages 197~206
An outer membrane-associated 2-furaldehyde dehydrogenase, catalyzing the oxidation of 2-furaldehyde to 2-furoic acid from Klebsiella pneumoniae was purified to homogeneity and characterized. The enzyme showed its highly specific dependency on
. Enzyme activity was monitored during purification by using substrate 2-furaldehyde and coenzyme
by means of high performance liquid chromatography. The outer membrane was successfully collected by the methods of Percoll density gradient ultracentrifugation and ultracentrifugation after preferential solubilization of the membrane with
and Triton X-100. The enzyme was purified by the series of procedures including extraction of outer membrane protein with EDTA and lysozume, and fractionation by column chromatography on QAE-Sephades Q-50, and subsequently Sephadex G-100. The enzume showed its optimal activity at
, pH 9.5, and in the presence of 1.5% (vol/vol) Triton X-100. The enzyme exhibited a native molecular size of 88,000 by nondenaturing polyacrylamide gel electrophoresis and had an apparent Km of 4.72mM for 2-furaldehyde.
Characterization of cytosine deaminase with substrate specificity to 5-fluorocytosine
The Korean Journal of Microbiology, volume 26, issue 3, 1988, Pages 207~214
A cytosine deaminase from the cell-free extract of an isolate was examined after ethyl alcohol reactionation. The enzyme catalyzed the conversion of 5-fluorocytosine to 5-fluorouracil by the possession of specificity to the substrate. The optimum temperature and storage time on the stability of the enzyme were at below
and near 2 days in tris-HCl buffer. The maximum activity was also presented ar 9.0 in pH and
in temperature. The pHs and temperatures for the enzyme activity ranged from 8.5-9.5 and from 40-
, respectively. the presence of
in the reaction mixture resulted in the marked inhibition in the activity, but 1mM of
increased the enzyme activity. The enzyme preparation was vot affected by inhibitors used except N-ethylmaleimide of 1 and 10mM, and considerably activated by 1mM of pyrophosphate and 10mM of phosphate.
Effect of Methionine Sulfoximine in nitrogenase activity by ammonia and glutamine in Rhodopseudomonas sphaeroides
The Korean Journal of Microbiology, volume 26, issue 3, 1988, Pages 215~222
The effect of ammonia and glutamine on nitrogenase activity of Rhodopseudomonas sphaeroides was examined. The nitrogenase activity of this strain was inhibited by ammonia and glutamine. When ammonia and glutamine were exhausted, nitrogenase activity promptly resumed at its original rate. Methionine sulfoximine (MSX), irreversible glutamine synthetase (GS) inhibitor, is a structural analogue of glutamate. MSX was used in order to know whether the nitrogenase activity was inhibited by ammonia and glutamine directly or not. The ability of MSX to prevent nitrogenase switch-off by ammonia was found to be dependent upon the phase of culture. When the cells were sampled after 12 hour culture,
MSX would not prevent the nitrogenase switch-off by ammonia. Twenty one percents of GS actibity was inhibited by
of MSX and concentration of released ammonia decreased. But nitrogenase activiy was still inhibited by ammonia. However, nitrogenase switch-off after 20 hours would be prevented by
of MSX. On the other hand, GS activity was ingibited completely by
MSX and concentration of released ammonia somewhat increased. But nitrogenase activity was not inhibited. The data indicated that the inhibition of in vivo nitrogenase actibity of Rp. sphaeroides by ammonia seemed to be mediated by products of ammonia assimilation rather than by ammonia itself.
-glucanase from Bacillus subtilis Using Chromogenic Substrate
The Korean Journal of Microbiology, volume 26, issue 3, 1988, Pages 223~229
Bacillus subtilis K-4-3, which produces considerable amount of
-glucanase was selected among extracellular
-glucanase-producing bacteria isolated from soil.
-glucanase was purified by ammonium sulfate fractionation, Sephadex G-100 gel filtration and DEAE-sephacel ion exchange chromatography. The purified enzyme revealed a single band by polyacrylamide gel electrophoresis and SDS-polyacrylamide gel electrophoresis. Its molecular weight was estimated to be 17000 dalton by SDS-polyacrylamide gel electrophoresis. The optimum pH and temperature of the purified
-glucanase were 7.0 and
, respectively. The enzyme was strongly inhibited by 1.0mM of
, and activated by 1.0mm of
. The absence of glucose after thin layer chromatography of reaction products revealed that the purified enzyme contains no cellobiase or laminarinbiase activity. The loberation of ki, tri-and tetra-saccharide as reaction products can be explained by endoaction of the enzyme.
Distributions and heterotrophic actibities of bacteria in Lake Paro
The Korean Journal of Microbiology, volume 26, issue 3, 1988, Pages 230~236
The distributions of bacterial numbers and activities were studied bimonthly in 1987, at 3 sites in Lade Paro for elucidating the changes by disturbance of aquatic ecosystem. The total bacterial number was
cells/ml. The geterotrophic bacterial number had the variance from
CFUs/ml and the variation trend was similar to that of the total bacterial number. The proportions of alpha-glucosidase or beta-glucosidase releasing bacteria showed temporal changes rather than spatial changes. The proportions of phosphatase releasing bacteria had the maximum values, 22.7-83.0%, in July. The electron transpory system activity revealed the variation from
/l/day and hagher values at upper stream and in summer. The degradation fraction by phosphatase was 0.4-9.1%/h and increased with temperature. The maximum value of heterotrophic activity was 8.2%/h in summer. Eventhough the distributions of total bacteria and heterotrophic bacteria were affected by the water disturbance but microbial activities and proportions of the specific enzume releasing bacteria were not affected.
Physicochemical factors affecting the adsorption of E. coli in estuarine sediments
The Korean Journal of Microbiology, volume 26, issue 3, 1988, Pages 237~246
The higher bacterial numbers on clay than on sand were caused by different environmental factors. Such factors affecting the adsorption of E. coli ATCC 11775 in the sediment as follows; optimal pH range for the adsorption of E. coli ATCC 11775 was pH 7.5-pH 9.5. E. coli ATCC 11775 were shown maxima in the salinity of 18.
on sand type sediment and
on clay type sediment. Bacteria attached better to clay typed sediment than to sand typed sediment when organic substance was eliminated. Beef extract of 0.5%-1% concentration was found to promote the attachment of E. coli ATCC 11775 effectively. Peptone of 0.5% was enganced the attachment on the clay, and peptone of 1.3%-5%, on the sand. E. coli ATCC 11775 was found to adsorb onto benthonite with the highest efficiency and on celite with the lowest efficiency. Efficiency of adsorption by inorganic ions was shown due to higher values of ion. Adsorption was achieved in the order of
Numerical Taxonomy of Heterotrophic Bacteria in Naktong Estuary
The Korean Journal of Microbiology, volume 26, issue 3, 1988, Pages 247~255
Using the numerical clustering method, 14 clusters (containing three or more strains) among 231 bacterial isolates from St. 1,17 clusters among 252 isolates from St.2,17 clusters among 301 isolates from St.3, and 15 clusters among 260isolates from St.4 were found at the 70% similarity value. The predominant organisms were identified as genera Aeromonas, Vibrio, and Alcaligenes at St.1, Alcaligenes at St.2, Aeromonas, Vibrio, and Moraxella-Acinetobacter group at St.3, and Pseudomonas at St.4.
Characteristics of Heterotrophic Bacteria and Their Relationships with Environmental Parameters in Naktong Estuary
The Korean Journal of Microbiology, volume 26, issue 3, 1988, Pages 256~261
Samples from Naktong Estuary had been taken for the characterization of heterotrophic bacterial communities and of the effects of environmental factors on their distribution in estuarine ecosystem. Bacterial communities isolated from seawater region were composed of more euryhalone groups than those from freshwater region, and the bacterial communities of summer were composed of more eurythermal groups than those of winter. Bacterial commnities became more diverse by the input of allochthonous bacteria from terrestrial and freshwater ecosystem, but less diverse by worse environmental conditions such as nutrient load, high salinity, low temperature, and so on.
Virulence characters of Yersinia enterocolitica isolated in Korea
The Korean Journal of Microbiology, volume 26, issue 3, 1988, Pages 262~269
In order to investigate the virulence of Y. enterocolitica isolated in Korea, all necessary experiments were done including several virulence determinating tests-autoagglutination test, calcium-dependency test, HeLa cell invasion test, Sereny test, crystal violet binding test, and electrophoresis for plasmid pattern. The obtained results are as follows: The virulent strains of Y. enterocolitica revealed positive reactions on autoagglutination test, cacium-dependency test, and drystal violet binding test, while the avirulent strains did not. A positive reaction was observed only at
implying that the expression of virulence is temperature-dependent. In Sereny test, the standard reference virulent strain (serotype 0:8) showed positive reactions while the virylent experimental strains (serotype 1:3, 0:9) revealed negative results, which indicates that the virulence of Y. enterocolitica in experimental animals varied according to their serotypes. Most of the virulent strains contained 36-38Mdal plasmids, but the avirulent strains did not. In addition, it was noted that autoagglutination, calcium-dependency, and crystal violet binding were related to the presence of plasmids.
L-phenylalanine ammonia-lyase activity of fungi, yeasts and streptomyces
The Korean Journal of Microbiology, volume 26, issue 3, 1988, Pages 270~277
Microorganisms isolated from soil (150 strains), fungi (39 strains), yeasts (9 strains) and Streptomyces species (39 strains) were assayed for L-phenylalanine ammonia-lyase(PAL) activity. 17 strains of fungi and 46 strains of soil isolates were proved to produce PAL, Aspergillus panamensis, Penicillium varioti and 11 soil isolates showed comparatively large PAL activity. When PAL activity was assayed with cell-free extracts of these 13 strains and 7 strains of Rhodotorula and Rhodosporidium geni, Rhodosporidium toruloides (IFO 0559) showed the highest PAL activity with 0.333 units per g of the wet cell weight.
Acetone-Butanol Fermentation of Rice Straw by Simultaneous Saccharification and Fermentation
The Korean Journal of Microbiology, volume 26, issue 3, 1988, Pages 278~282
Rice straw was used in the acetone-butanol fermentation by semultaneous saccharification and fermentation (SSF) using Clostridium acetobutylicum and cellulolytic enzyme. Over 230 mM of solvent was produced from alkali treated rice straw of from ball-milled microcrystalline cellulose whilst only acidic fermentation products were formed from ball-milled rice straw. From the results it is concluded that rice straw used in the study contained an inhibitor for the solventogenesis by the organism which is insoluble in water and some organic solvent and destroyed by alkaline treatment.