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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
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Volume & Issues
Volume 26, Issue 4 - Dec 1988
Volume 26, Issue 3 - Sep 1988
Volume 26, Issue 2 - Jun 1988
Volume 26, Issue 1 - Mar 1988
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Characterization of a Revertant that Restroes the Export of Ribose-Bnding Potein to the Priplasm in Echerichia coli
;;Randall, Linda L.;
The Korean Journal of Microbiology, volume 26, issue 4, 1988, Pages 283~290
A spontaneous revertant of mutation rbsB103 that is ribose taxis-positive was characterized. This revertant was found to be export-competent in the export of ribose-binding protein shown by the disappearance of accumulated mutant precursor protein and the export of mature ribose-binding protein to the periplasm. The reversional change was shown to be in the region of risB gene that codes for the amino terminal portion of ribose-binding protein. Analysis by high-performance liquid chromatography of peptide patterns of ribose-binding proteins confirmed the relationship between the wild-type and the revertant proteins as shown for the mutant previously (Iida et al., 1985). When the processing rate of presursor proteins from the wild type and the revertant strain in vivo was compared by pulse-chase experiment, it was found that processing is less efficient than normal in the revertant. Purified mature proteins from both wild-type and revertant were subjected to amino acid sequencing. The results confirmed the amino acid changes deduced from the DNA sequencing and showed that processing of the revertant precursor occured in the correct position even though there are two different amino acids present in the signal sequence.
Overproduction and Purification of Ribose-Binding Proteins from the Wild-Type Mutant and Revertant Strains in Escherichia coli
;Randall Linda L.;
The Korean Journal of Microbiology, volume 26, issue 4, 1988, Pages 291~297
Three alleles of rbsB gene, rbsB, rbsB103, and rbsB106 from the wild type, the mutant and the revertant strain, respectively, were cloned for overproduction of proteins under the control of lambda
promoter. Five different species of precursor and mature ribose-binding proteins were purified to homogeneity using DEAE-Sephadex column chromatography, osmotic shock pocedure, CM-Sephadex column chromatography, and Chromatofocusing column chromaography. pI of the precursor proteins and mature proteins were determined and found to be pH 8.0 and 7.5, respectively. The purified proteins were subjected to amino acid sequencing. The results confirmed the amino acid changes deduced from the DNA sequencing.
Spheroplast fusion of pseudomonas spp. using plasmid as selective marker
The Korean Journal of Microbiology, volume 26, issue 4, 1988, Pages 298~304
Antibiotic resistance plasmids (RP4, pMG1, R679 and R91-5) were used as primary selective markers to detect the fusants in Pseudomonas spp. By using plasmid marker, clones containing both plasmids of parental strains were obtained as fusants by direct selection. The spheroplast fusion was occured not only between strains of interspecies, but also between strains of intergenus such as Pseudomonas and E. coli. The frequencies of fusant formation were variable from
-4/. In addition, chromosomal recombinants were formed among the clones with both parental plasmids in frequencies of 0.44-1.3%. The fusant formation frequency between interspecies of intraspecies was not different markedly, but stability of plasmids in fusants correlated with the phylogenic smilarity of the parental strains.
Mutagenesis of Slow Growing Rhizobium japonicum by Transposon Tn5
The Korean Journal of Microbiology, volume 26, issue 4, 1988, Pages 305~311
The spectinomycin resistant strain of slow growing R. japonicum R-168 was selected to be participated in conjugation with E. coli WA803/pGS9. Tn5 was introduced from suicide vector pGS9 into R. japonicum R-168
chromosome at the frequency of
and the transconjugante were selected on the yeast extract-mannitol plate containing kanamycin (
g/ml) and spectinomycin (
g/ml) after 8-9 days incubation. All transconjugants we tested were found to contain Tn 5 DNA on their genome, which was confirmed by Southern hybridization experiments. R. japonicum RNa75, which had been selected through plant test, was found to be defective in symbiotic nitrogen fixing ability and the production of leghemoglobin in soybean nodules formed by the inoculation of this mutant. In addition, this mutant strain hardly developed nitrogenase activity asymbiotically in contrast with the wild type strain, indicating that some nitrogen fixing gene might be blocked in this strain and the production of leghemoglobin could be decreased by the interference in nitrogen fixing genes.
Studies on the riboxomal RNA genes of rhizobium meliloti and bradyrhizobium japonicum
The Korean Journal of Microbiology, volume 26, issue 4, 1988, Pages 312~317
The genes for ribosomal RNA in Rhizobium meliloti and Bradyrhizobium japonicum were analyzed by southern hybridization of BamHI, EcoRI, HindIII digested chromosomal DNA with purified 5'
-labeled 16S and 23S rRNA. The big differences in the hybridization pattern of both rhizobia were found. The comparative results were discussed in relation to the copy number and conservativity of restriction sites in the rRNA genes of both rhizobia.
Localization of MAK18 gene on chromosome VIII of saccharomyces cerevisiae
The Korean Journal of Microbiology, volume 26, issue 4, 1988, Pages 318~323
MAK18 gene of Saccharomyces cerevisiae, needed for M1 replication, was mapped within 2cM of PET3 on chromosome VIII. From 38kb clone pRE66 carrying SPO11 and PET3, we have localized MAK18 gene whose insert is 2.8kb. MAK18 gene is Iocalized on about 9kb distance from PET3 and about 18kb distance from SPO11 on chromosome VIII.
Properties of Ascorbate-Oxidizing Enzyme Purified from Pleurotus ostreatus
The Korean Journal of Microbiology, volume 26, issue 4, 1988, Pages 324~331
Ascorbate oxidizing enzyme from the crude extract of Pleurotus ostreatus was purified by ammonium sulfate precipitation, preparative polyacrylamide gel electrophoresis, DEAE Sepharose CL-6B ion exchange chromatography and Sephadex G-150 gel filtration chromatography. The molecular weight of the enzyme estimated by Sephadex G-150 gel filtration chromatography was 140,000 and that of its subunit by SDS-polyacrylamide gel electrophoresis 66,000. The optimum pH for the maximum activity of the enzyme was 5.2 and the isoelectric point of the enzyme was 6.0 Km values for L-ascorbic acid and D-isoascorbic acid were both 2.2.
M, which indicates that the enzyme has the asme affinity towards both substrates.
Bacterial Abundance and Heterotrophic Activity in Sudong Stream
The Korean Journal of Microbiology, volume 26, issue 4, 1988, Pages 332~338
The density of heterotrophic bacterial population and heterotrophic activity were measured at monthly interval from March, 1986 to March, 1987 at four sites in the Sudong Stream, a tributary of North Han River. Total bacterial numbers and maximum uptake velocity (
) of glucose ranged as 0.8-
of glucose showed marked seasonal periodicity, with highest values in summer. But density of viable bacteria varied considerably, with no definite seasonal pattern. At the nucontaminated site which located in upstream, heterotrophic bacterial population and activities were relatively low, and small variations were observed downstream flowing except the site where animal originated sewage inputs occurred. And this large input of allochthonous materials and bacteria was an important factor for the stream condition.
Effect of temperature and salinity on the bacterial degradability of petroleum hydrocarbon
The Korean Journal of Microbiology, volume 26, issue 4, 1988, Pages 339~347
The rate of bacterial degradation of hydrocarbon was estimated for the measurment of the self-purification capacity of the aquatic ecosystem. Strain ND601P-2, selected as petroleum degrading bacteria from Nakdong River Estuary with high degradability of petroleum, transformed 42% of hexadecane to
or cell mateials under the conditions of
, 0.03M NaCl, 167mg-
/1, 50 mg-hexadecane/1. The mineralization rate was found to be significantly affected by the temperature and the
value was 2.2. Teh optimal salinity of the strain ND601P-2 was 2o/oo. The increased salinity caused the elevation of % respiration value and the prolonged lag phase.
Effects of physical and chemical factors on the growth of Tetrahymena sp. isolate
The Korean Journal of Microbiology, volume 26, issue 4, 1988, Pages 348~354
A Protozoa, Tetrahymena sp. was isolated from domestic wastewater and identified by morphological characteristics. It was 50 to
in length, ovoidal shape, and divided by binary fission. When Escherchia coli was fed as a food microorganism, the optimal conditions for growth were 30 to
, pH 7 to 9 and 0.05% NaCl. The growth was inhibited by heavy metal ions; 0.3 mg of
and 0.1mg of
. While, it was not inhibited up to 0.7 mg of
Relationship between Sporulation and Synthesis of Alkaline Protease in Streptomyces sp.
The Korean Journal of Microbiology, volume 26, issue 4, 1988, Pages 355~361
The aims of the present studies were to understand the physiolosical and genetic characters of Streptomyces sp. isolated from soil. It revealed that Streptomyces sp. SMF301 had very fast growth rate and produced extracellular protease and heavily sporulated on rich media. It also showed
-lactamase activity and pigment production. Nonsporulating mutants were isolated after NTG or acriflavin treatment and their characters were compared with the parent strain. It was found that the mutants obtained by acriflavin treatment and ghier characters were compared with the parent strain. It was found that the mutants obtained by acriflavin treatment lost the pigment formation and
-lactamase production. Protease actibity of the mutant was lowered and the pH optimum was changed toward neutral. It was found that the changes were resulted from the reduction of alkaline protease biosynthesis in the bald mutant. Therefore it is considered that sporulation, pigment formation,
-lactamase production, and alkaline protease production in Streptomyces sp. might be controlled with a closely related relationship.
Optimum culture conditions for production of extracellular cytosine deaminase by bacellus polymyxa YL 38-3
The Korean Journal of Microbiology, volume 26, issue 4, 1988, Pages 362~367
The strain YL 38-3, which was capable of producing extracellular cytosine deaminase, was isolated and taxonomically examined. The isolated strain was identified to be Bacillus polymyxa YL 38-3. The optimal conditions for the enzyme production from Bacillus polymyxa YL 38-3 were investigated. The enzyme production was reached maximum level in the medium containing 0.5% glucose, 0.2% beef extract, 0.5% NaCl and 0.1%
(pH 6.0). And the enzyme showed the highest activity when the strain YL 38-3 was cultivated at
for 24 gours under the initial pH 6.0. By the additions of peptone the extracellular enzyme production was inhibited, meanwhile the intracellular enzyme production was highly stimulated. It was, therefore, deduced that peptone was related to the secretion mechanism of the enzyme from this bacterial cell.
Enzymatic Properties of Extracellular Cytosine Deaminase
The Korean Journal of Microbiology, volume 26, issue 4, 1988, Pages 368~374
Enzymological proprties of an extracellular cytosine deaminase from Bacellus polymyxa YL 38-3 were investigated. The extracellular enzyme was very stable, and optimum pH and temperature for the enzyme activity were found to be near pH 6.0 in 0.2M potassium phosphate buffer and at
, respectively. 5-Fluorocytosine was converyed to 5-fluorouracil by the enzyme, but 5-methylcytosine was not to thymine by it. The enzyme activity was completely inhibited by some heavy metal ion such as 1mM of
, and by 1mM of p-chloromercuribenzoate, respectively. The enzyme activity was inactivated about 75% by 1mM of o-phenanthroline and monoiodoacetate. But the enzyme activity was stimulated up to 200% by 1mM of 2-mercaptoethanol.