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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
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Volume & Issues
Volume 27, Issue 4 - Dec 1989
Volume 27, Issue 3 - Sep 1989
Volume 27, Issue 2 - Jun 1989
Volume 27, Issue 1 - Mar 1989
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Cloning of RNA1 Gene from Saccharomyces cerevisiae
The Korean Journal of Microbiology, volume 27, issue 2, 1989, Pages 77~84
The temperature sensitive (ts) mutation on RNA1 gene of Saccharomyces cerevisiae prevents growth at restrictive temperature (
) by accumulation of precursor tRNA, rRNA and mRNA (Hutchison et al., 1969; Shiokawa and Pogo, 1974; Hopper et al., 1978). RNA1 gene was cloned by complementation of the temperature sensitive growth defect of an rna1-1 mutant strain and identified by retransformation and concomitant loss of recombinant plasmid on non-selective condition. By deletion mapping, it was found that RNA1 gene resides within 3.5kb of BgII fragment.
Effect of Viral Enhancers on the Tissue-Specific Expression of Bovine Growth Hormone Gene
The Korean Journal of Microbiology, volume 27, issue 2, 1989, Pages 85~91
The effect of SV40 and murine cytomegalovirus (MCMV) enhancers on the general and tissue-specific gene expression was investigated. Recombinant plasmids containing these transcriptional engancers downstream of a structural gene for chloramphenicol acetyl transferase (CAT) were constructed. The transient expression of CAT gene from these plasmids was measured in monkey (CV1PD) and HeLa cells. Both SV40 and MCMV engancers activated the expression of CAT gene by more than 20 and 150 folds, respectively, compared with engancerless plasmids. When the SV40 promoter, upstream of CAT gene, was replaced with 2.2 kbp of promoter regulatory region of bovine growth hormone (bGH) gene, there was no expression of CAT even in the presence of enhancers, reflecting the tissue-specific expression of bGH genes. However, when the bGH regulatory region was shortened to 230 bp, the expression level increased dramatically in the presence of SV40 enhancers. In contrast, the expression from the shortened promoter was only marginally activated by the stronger MCMV enhancer.
Construction of rhizobium-E. coli shuttle vector using replication and mobilization function of indigenous multicopy plasmid from rhizobium
The Korean Journal of Microbiology, volume 27, issue 2, 1989, Pages 92~97
the vector, pGUR19, for Rhizobium gene manipulation, was constructed by combining the replication and mobilization function of indigenous multicopy plasmid from Acacia(Robinia pseudoacacia L.) Rhizobia sp86 with E. coli cloning vehicle, pBR322. The vector could be efficiently mobilized by RP4 tra function incorporated into chromosome of E. coli named SM10 and efficiently transferred to various gram negative hosts including Rhizobium and Afrobacterium by transformation. Mobilization frequency of the constructed vector was ranged from
(E.coli HB 101) to
(A. tumefaciens 15955) and transformation frequency was ranged from
(E. coli HB101) to
(A. tumefaciens 15955). The vector, pGUR19, was stably replicated and maintained in a variety of Rhizobium and Agrobacterium.
Analysis of Intraspecific Protoplast Fusion Products in Trichoderma koningii
The Korean Journal of Microbiology, volume 27, issue 2, 1989, Pages 98~107
Intraspecific fusants, produced by protoplast fusion of auxotrophic mutants from Trichoderma koningii ATCC 26113, were segregated into various strains including parental types, non-parental auxotrophic hybrids, and prototrophic hybrids on complete plate. Interestingly, some of non-parental prototrophic hybrids revealed to have enhanced cellulolytic activity incomparison with other strains of parents or hybrids derived thereafter. It was also evident that prototrophic hybrids of aneuploid could be constructed after the spontaneous segregation of complementing fusants produced through the protoplast fusion.
Affinity labeling of the Vacuolar Arginine Transporter in Neurospora crassa
;Weiss, R. L.;
The Korean Journal of Microbiology, volume 27, issue 2, 1989, Pages 108~116
Based on the specificty of recognition of the vacuolar arginine transporter, N-p-nitrobenzoxycarbonyl (NBZ)-L-arginyl diazomethane was synthesized and used as an affinity label specific for the arginine transporter. This arginyl derivative ingibited both ATP-dependent and independent L-arginine transport into vacuolar membrane vesicles. When vacuolar proteins were labeled with radioactive NBZ arginyl diazomethane, the binding was irreversible, detached by treatment with base and blocked by treatment with cysteinyl blocking groups suggesting cysteine as a labeling site.
Purification of the Vacuolar Arginine Transporter from Neurospora crassa
;Weiss, R. L.;
The Korean Journal of Microbiology, volume 27, issue 2, 1989, Pages 117~123
H] arginyl diazomethane was used as an affinity label for the vacuolar arginine transporter in Neurospora crassa. Vacuolar matrix proteins were removed by fracturing the membranes with freeze-thaw method in dry ice/ethanol bath. Vacuolar membrane proteins were then wasged with 500mM NaCl to remove ionically bound derivatives and peripheral membrane proteins from vacuolar membranes. After dissolved in 1% Titon X-100, dissolved vacuolar memvrane proteins were separated with molecular sieve column chromatography, anion and cation exchange chromatographies. The arginine transporter was purified giving the purification factor of 1136.
Alginic acid production of azotobacter vinelandii
The Korean Journal of Microbiology, volume 27, issue 2, 1989, Pages 124~129
In order to improve the production of bacterial alginate, Azotobacter vinelandii NCIB 8789 was treated with 200.
g/ml of MNNG for obtaining mutant strain. The mutant HB18 was selected, which produced the highest amount of alginic acid among the survival stains. The HB18 produced 5.4g/l of alginic acid when batch cultured at
for 160 hrs and its alginic acid showed high molecular weight and simple composition when compared with thoseof wild type.
Distribution of bacterial biomass in the water column of Soyang lake
The Korean Journal of Microbiology, volume 27, issue 2, 1989, Pages 130~138
Microbiological parameters such as bacterial biovolume and biomass in Soyang Reservoir was statistically analyzed with the physico-chemical enviromental factors. Analysis of correlation and multiple regression showed that temperature affects most of microbiological parameters. Variations of total bacterial number, total bacterial biovolume and saprophyte number were highly correlatd with the concentrations of chlorophyll a and pheophytin a. Bacterial production by the
-thymidine incorporation rate was largely affected by Seston. It suggests that microbiological factors such as bacterial biovolume and bacterial biomass were controled by the concentration of seston and distribution of phytoplankton which acts as carbon and energy source for the bacterial community in the water column of Soyang Reservoir.
Microbial Degradation of Diazinon in Sudmerged Soil
The Korean Journal of Microbiology, volume 27, issue 2, 1989, Pages 139~146
The mechanisms and metabolic products involved in the degradation of an organophosphate insecticide, diazinon, were studied in submerged paddy soil under the laboratory condition at
. Diazinon abatement in non-sterilized soil was more rapid than indicating microbial participation in diazinon in soil. One-half of the original applications was lost in 2 days and less than 5% remained after 7 days. During the same period, dizinon applications increased tha microbial populations in accordance with the monooxygenase and esterase activities in soil. These results suggest that the microbiological factors develop in soil following diazinon application. The esterase and monooxygenase-catalyzing degradation products of diazinon were isolated and tentatively identified by mass spectrometryas 2-isopropyle-6-methyl-4-hydroxy pyrimidine, diazoxon, hydroxydiazinon, and sulfotep.
Biological properties of vibrio vulnificus lipopolysaccharide and compared to those of escherichia coli and salmonella typhimurium lipopolysaccharides
The Korean Journal of Microbiology, volume 27, issue 2, 1989, Pages 147~154
Vibrio vulnificus Lipopolysaccharide (LPS) was extracted, performed chemical analysis, tested its biological activities, and compared to those of Escherichia coli LPS and Salmonella typhimurium LPS. The lethal activity of V. vulnificus LPS was 138.6138.6 mg/kg in mouse, but this was lower than thowe of E. coli LPS (56.3 mg/kg) and S. typhimurium LPS (37.5 mg/kg). The result of fatty acid analysis showed that V. vulnificus LPS had more saturated fatty acid than E. coli LPS and S. typhimurium LPS. Above results indicated that V. vulnificus LPS did not have much effect on the lethality. The results of biological responses of enzymes and blood cells by LPSs showed that V. vulnificus LPS had slightly greater activity than E. coli LPS and S. typhimurium LPS. V. vulnificus LPS was recommendavle for stimulant on interferon induction because of adequate stimulation and safety for host and cell lines.
Development of Lactobacillus sporogenes Resistant to Rifampicin an Antituberculosis Agent
The Korean Journal of Microbiology, volume 27, issue 2, 1989, Pages 155~161
Lactobacillus sporogenes was treated with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) to obtain resistant mutants to rifampicin. Fifty-eight strains of the NTG-induced mutants showed distinct resistance to rifampicin and nine mutants were selected for further studies. They also exhibited identical characteristics with the parent Lactobacillus sporogenes when they were tested for spore formation, acid formation and growth inhibition of E. coli. From in vitro test it was identified that rifampicin is not inactivated by certain factors of the rifampicin resistant mutants. It is suggested that they can be utilized as efficient normalizing agents for human intestinal flora when they are simultaneously taken with rifampicin
A small recA analog in streptococcus pneumoniae that is not induced during competence for genetic transformation
;Morrison, Donald A.;
The Korean Journal of Microbiology, volume 27, issue 2, 1989, Pages 162~167
Western blot analysis of lysates of Streptococcus pneumoniae revealed a single polypeptide species that cross-reacted with E. coli RecA antiserum. The apparent molecular weight of this putative RecA protein analog (RecAsp) was 24, 000 smaller than any other known RecA analogue. The RecAsp protein was present at the same level in competent and non-competent cells.