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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 27, Issue 4 - Dec 1989
Volume 27, Issue 3 - Sep 1989
Volume 27, Issue 2 - Jun 1989
Volume 27, Issue 1 - Mar 1989
Selecting the target year
Selective overproduction of chloramphenicol acetyltransferase in the T7 expression system
The Korean Journal of Microbiology, volume 27, issue 4, 1989, Pages 317~322
A gene can be selectively overexpressed in E. coli by utilizing the phage T7 RNA polymerase's stringent recognition and active transcription of the T7 promoter. The T7 expression system was constructed such that the T7 RNA polymerase gene is under the control of lacUV5 promoter in one plasmid, and that the target gene, the promoterless chloramphenicol acetyltransferase (CAT) gene with E. coli ribosome binding site is under the control of T7 promoter in the other plasmid. Only the E. coli cells containing both plasmids show high resistance to chloramphenicol. When the copy number of the runaway plasmid containing the polymerase gene was varied by a temperature shift, amounts of the CAT protein synthesized upon induction was correspondingly changed as shown in SDS gel electrophoresis.
Amplified synthesis and stability of Tn5 polypeptides in escherichia coli
The Korean Journal of Microbiology, volume 27, issue 4, 1989, Pages 323~333
Plasmid DNA molecules containing strong promoter upstream from IS50L or IS50R, the two insertion sequences that flank Tn5, were constructed to amplify the synthesis of Tn5-encoded polypeptides. When proteins made by cells that contain these plasmids were analyzed on polyacrylamide gels, enhanced synthesis of IS50R polypeptides could be detected. Synthesis of this polypeptide apparently is initiated within the large open reading frame of this element. In addition, the stability of IS50L-and IS50R-encoded polypeptides was analyzed. It was found that IS50L polypeptides are relatively unstable in vivo. This instability could account for the observed inability of this element to promote transposition.
Characterization and Incompatibility of Transmissible TOL Plasmid from Pseudomonas cepacia
The Korean Journal of Microbiology, volume 27, issue 4, 1989, Pages 334~341
Toluate degradative plasmid from Pseudomonas cepacia SUB37 was determined to be molecular weight as $79.3\times 10^6$
Chromosomal Studies on the Genus Fusarium
The Korean Journal of Microbiology, volume 27, issue 4, 1989, Pages 342~347
by use of HCl-Giemsa technique and light microscope, dividing vegetative nuclei in hyphae of Fusarium species were observed and the results are summerized. The chromosome number of these fungi was ranged 4 to 8. Of the 20 strains, the highest haploid chromosome number is 8 in F. solani S Hongchun K4, F. moniliforme (from banana) and F. raphani (from radish). The lowest is 4 in F. sporotrichioides NRRL 3510 and F. equiseti KFCC 11843 IFO 30198. F. solani 7468 (from Sydney), F. solani 7475 (from Sydney), F. oxysporum(from tomato). F. roseum (from rice), F. sporotrichioides C Jngsun 1, F. equiseti C Kosung 1 and F. avenaceum 46039 are n=7. F. moniliforme (from rice) F. graminearum, F. proliferatum 6787 (from Syndey), F. proliferatum 7459 (from Synder) and F. anguioides ATCC 20351 are n=6. F. moniliforme NRRL 2284, F. poae NRRL 3287 and F. trincinctum NRRL 3299 are n=5. From these results, it may be concluded that the basic haploid chromosome number of the genus Fusarium is 4 and mat have been evolutionary variation of chromosome number through aneuploidy and polyploidy.
Characterization of Extracellular Peroxidase from Pleurotus ostreatus
The Korean Journal of Microbiology, volume 27, issue 4, 1989, Pages 348~356
An extracellular peroxidase found in culture broth of Pleurotus ostreatus was induced by syringic acid. This enzyme was fractionated by DEAE Sephadex A-50 ion exchange chromatography and gel filtration chromatogrphy on Sephadex G-150. The enzyme is a glycoprotein containing 35.7% carbohydrate. The results of SDS-linear polyacrylamide gradient gel electrophoresis and gel filtration indicate that the enzyme is a dimer consisted of identical subunits (Mr=72,400). The absorption spectrum of the enzyme indicates the presence of one mole of iron protoporphyrin IX per one mole of subunit. Isoelectric point of the enzyme is 4.26 and
. The enzyme showed its optimal activity at pH 3.5-4.0 and at
. The Km values of this enzyme for ferulic acid and sinapic acid are 2.4 and 12.3 times higher than those of horseradish peroxidase, respectively.
Induction of Autolysis and Autoplast Formation of Anaerobic Clostridium thermohydrosulfuricum
The Korean Journal of Microbiology, volume 27, issue 4, 1989, Pages 357~365
Induction conditions for autolysis and autoplast formation of thermophilic Clostridium thermohydrosulfuricum were studied. The cells in the initial exponential growth phase were well autolysed in Tris-HCl buffer or inorganic buffers containing univalents, such as
, and chemicals such as cysteine-HCl, sorbitol and glycerol. Meanwhile, autolysis induction was slightly inhibited by divalents, such as
, and strongly by divalents, such as
and citric acid. The autolysis was stimulated when the cells were grown in the medium containing ampicillin that inhibites cell wall synthesis, meanwhile, it was slightly inhibited by nucleic acids and protein synthesis inhibitors. The optimal pH and temperature for the induction of autolysis were 7.5 and
, respectively. On the other hand, the cells were autoplasted without lysozyme treatment during autolysis due to the stabilization of protoplasmic membrane in the presence of divalents such as
. Autoplast formation was mostly induced at
in 50mM Tris-HCl buffer (pH 7.5) containing 20 mM
and 0.3 M glycerol, and in the late exponential growth phase growing cell.
Induction of Steroid 11
-hydroxylase in Pellicularia fillamentosa
The Korean Journal of Microbiology, volume 27, issue 4, 1989, Pages 366~372
Twenty-one strains were tested for 11
-hydroxylation of Reichstein's substance S. Four fungi exhibited ability for the reaction, among which Pellicularia fillamentosa showed the highest activity. The 11
-hydroxylase of this fungus was proved to be induced by the substrate, cycloheximide reducing significantly the activity of the enzyme. Range of optimum pH for the 11
-hydroxylation was broad and found to be 2.0-8.0. Test of the enzyme activity at different growing stages, from spore to mycelia, showed that the branching stage of hyphae and the mature mycelial stage were the most effective for the Reichstein's substance S transformation. However, 11
-hydroxylase in the intact spore was turned out to be uninducible with the substrate.
Effects of environmental factors on the extracellular release of photosynthetic products by scenedesmus quadricauda
The Korean Journal of Microbiology, volume 27, issue 4, 1989, Pages 373~377
The effects of environmental factors on the extracellular release of organic carbon by Scenedesmus qudricauda were studied. The PER (percentage extracellular release) was greater at high temperature and at high concentration of nitrogen and phosphate. The PER variation according to the change of M/P ratio showed high values at each extreme N/P ratio. This result suggested that the limitation of nitrogen or phosphorous resulted in the accumulation of carbohydrates as photosynthetic products, and the products in high concentration were excreted through algal cell membrane.
Seasonal analysis of heterotrophic bacterial community in lake Soyang
The Korean Journal of Microbiology, volume 27, issue 4, 1989, Pages 378~384
The numerical taxonomy of heterotrophic bacterial community in Lake Soyang was analysed. 95, 115, 88 and 75 strains which were isolated at each season from spring in 1987 to winter in 1988 were clustered by single matching coefficient. The diversity indices (H') were in the range of 0.511-1.684, and the community was most diverse in spring. THe seasonal variation of generic composition was significant. Of the domonant genera, Acinetobacter, Pseudomonas, and Flavobacterium were representative.
Characteristics and Numerical Taxonomy of Aeromonas spp. Isolated from Naktong Estuary
The Korean Journal of Microbiology, volume 27, issue 4, 1989, Pages 385~390
One hundred and sixty one strains of Aeromonas were isolated at three sites from August, 1986 to December, 1986 in Naktong Estuary. Cluster analysis was performed on total of 42 morphological and biochemical characteristics of the isolated strains. At the level of 85% and 84% similarity, three major clusters and two minor clusters had been identified: the first three clusters were different one another from MR reaction and gas production utilizing glucose. The five clusters were classified as two serotypes on the basis of serotyping, which was consistent with the result of cytotoxicity test.
Effects of Environmental Factors on Aeromonas spp. Population in Naktong Estuary
The Korean Journal of Microbiology, volume 27, issue 4, 1989, Pages 391~397
Population of Aeromonas and environmental parameters were investigated at three sites from August 1986, to December, 1986 in Naktong Estuary. The variation range of Aeromonas was
MPN/100ml. The result of ANOVA indicates significant differences among the populations of Aeromonas in each site. The highest population of Aeromonas occurred at site 2, and the lowest at site 3-B. To scrutinize the effects of environmental parameters on the distribution of Aeromonas spp, principal component analysis and multiple stepwise regression were used. The results showed that distribution of Aeromonas spp. was mainly influenced by outflow of freshwater and inflow of inorganic nutrients and correlated with heterotrophic bacteria, available nitrogen, fecal coliform bacteria, and temperature.
Isolation of Symbiotic Rhizobium spp. Strain from Root Nodule of Canavalia lineata
The Korean Journal of Microbiology, volume 27, issue 4, 1989, Pages 398~403
The root nodule of Canavalia lineta was classified as a determinate nodule and the symbiont as a Rhizobium-bacteriod based on their morphological characteristics. Isolated encosymbiont was similar both to R. leguminosarum and R. meliloti in its peritrichous arrangement of flagella and some of the physiological characteristics. Compared to control plants, Canavalia seedlings inoculated with the isolate grew normally due to induced root nodules, confirming isolate's infectivity and effectivity. Characteristics of the reisolated endosymbiont from induced root nodule were identical to those of the first isolate, indicating the nodules were induced by the first isolate. From these results, it was confirmed that Rhizobium strain isolated from the root nodules of Canavalia lineata was a real symbiont, and was named Rhizobium sp. SNU003.
Reevaluation of Isolation and Identification of Gram-positive Bacteria in Kimchi
The Korean Journal of Microbiology, volume 27, issue 4, 1989, Pages 404~414
Attempts were made to isolate and identify Gram-positive or lactic acid bacteria in Kimchi fermentation. Species diversity depended on isolation media and temperatures, and diversity tended to be reduced with decrease of temperature. MRS and KM (natural medium prepared from Kimchi materials) were suitable respectively for isolation and present number of species. Identification of isolates was performed by dichotomous identification schemes arranged on the basis of Bergey's manual of Systematic Bacteriology (1986). Gram-positive bacteria isolated at different temperatures (5, 15,
) were 5 species of Leuconostoc, 4 species of Streptococcus, 3 species of Pediococcus, 2 species of Bacillus and 18 species of Lactobacillus. Species with high frequency of appearance were Lactobacillus plantarum, Streptococcus raffinolactis, Leuconostoc mesenteroides subsp. mesenteroides at
, L. plantarum, Lactobacillus fructosus, L. mesenteroides subsp. mesenteroides at
and L. mesenteroides subsp. mesenteroides, Leuconosotoc paramesenteroides, Lactobacillus maltaromicus at
. In general, Kimchi fermentation was achieved by Lactobacillus spp. (59.7% frequency) at
and Leuconostoc spp. (65.2% frequency) at
. Pediococcus cerevisiae and Streptococcus faecalis which have been so far known as bacteria of Kimchi fermentation were not isolated.
Isolation of the killer yeasts and its characteristics
The Korean Journal of Microbiology, volume 27, issue 4, 1989, Pages 415~421
Ten strains out of about 1,000 yeast strains isolated from byproducts of alcoholic industries, milk products, fruits, greens, food-related industries and soils of nature, revealed the killer activities. Two strains which have excellent killer activities among them were isolated and identified with Saccharomyces cerevisiae B 15-1 and Hansenula anomala Y 33 by investigation of the morphological, cultural and physiological properties. The optimal conditions on these strains for the production of killer toxin were investigated. The strain B 15-1 showed the highest killer toxin activities when it was cultured up to the log phase of 48 hr in YPD medium (pH 4.7) at
. On the other hand, the strain Y33 revealed the highest activities when it was cultured up to the stationary phase of 60 hr in YPD medium (pH 4.0) at
. The sensitive strain Kyokai 7 was found to be killed entirely by the killer toxin produced from the wild killer yeast B 15-1 when B 15-1 was cocultured with the same cell concentration (
cells/ml) of Kyokai 7 after cultivation of 36 hr, and with large concentration (
cells/ml) after 48 hr.
Conditions for protoplast formation and fusion of the killer yeast
The Korean Journal of Microbiology, volume 27, issue 4, 1989, Pages 422~429
Auxotrophic mutant were isolated from wild types by the treatment with NTG as a mutagen, and the conditions of protoplast formation for them were established. The protoplasts of killer yeast Saccharomyces cerevisiae K52 were formed to the level of above 70% when cells grown for 20 hr in PM medium were treated with 200 unit/ml Lyticase 50,000 at
for 60 min after pretreatment of 50 mM 2-mercaptoethanol in 10mM potassium phosphate buffer (pH 7.5) containing EDTA and 0.6 M sorbitol for 15 min. Also, the protoplast of the recipient S. cerevisiae S 29 were formed to the level of above 85% as it was cultured to the log phase of 24 hr in PM medium under the same conditions. The fusion frequency between the protoplast of killer yeast S. cerevisiae K 52 and the protoplast of recipient S. cerevisiae S 29 was reached to
when the hypertonic regeneration medium embeded with the fused protoplasts after mixing the parental protoplasts to 10
cells/ml in SP buffer containing 20 mM
and 30% PEG 6,000 for 15 min at
Effect of dextranase and glucose-oxidase on the formation of plaque by Streptococcus mutans
The Korean Journal of Microbiology, volume 27, issue 4, 1989, Pages 430~435
Dextranase and glucose-osidase was investigated as an anti-plaque agent and a component of dentifrice. In vitro synthesis of the water-insoluble glucan was decreased with increasing amount of dextranase and glucose-oxidase. Dextranase was effective on the decrease of viable S. mutans, and the formation of plaque decreased. But it is not effective on the degradatio of plaque. As a research for addition of enzyme to the dentifrice components, we formulated the Model Dentifrice for stabilization of enzyme. At the Model Dentifrice, we confirmed the stability of enzyme by evalution of activity for a long time.
A rapid method for sodium dodecyl sulfate polyacrylamide gel electrophoresis evaluation
Page, C. ; Hamelin, C. ;
The Korean Journal of Microbiology, volume 27, issue 4, 1989, Pages 436~438
We propose a method for rapid evaluation and permanent record of sodium dodecyl sulfate polyacrylamide gel electrophoretic runs. This method is based on the photocopy process, rather than on photography and requires no extensive or expensive investment. Comparison of a print obtained through this method and a 35mm photography indicated, on a balanced gel, equal sensitivity.