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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 28, Issue 4 - Dec 1990
Volume 28, Issue 3 - Sep 1990
Volume 28, Issue 2 - Jun 1990
Volume 28, Issue 1 - Mar 1990
Selecting the target year
The ascorbic acid oxidizing activity in streptomyces viridochromogenes
The Korean Journal of Microbiology, volume 28, issue 4, 1990, Pages 283~289
The competence time of Streptomyces viridochromogenes for aerial mycelium formation was determined. Within 10 hrs after spore inoculation the submerged mycelium was programed to form aerial mycelium, when the former was laid on agar plate. The white aerial mycelium was formed 17-22 hrs after the transfer. Ascorbic acid oxidizing enzyme band on native gel showed chracteristic mobility change during aerial mycelium formation. Total activity of this enzyme did not show any correlation with the differentiation. The asay condition for the crude enzyme was determined. EDTA and
showed stimulatory effect. Approximate ratio of oxygen consumed to ascorbic acid oxidized was 1:1.
Polyamines in methylobacterium extorquens AM1 grown on different energy sources
The Korean Journal of Microbiology, volume 28, issue 4, 1990, Pages 290~296
Putrescine, spermidine, and spermine were found to persent in Methylobacterium extorquens AM1 growing on methanol, succinate, glucose, or nutrient broth as an energy source. Spermidine was found to be a major polyamine in cells growing on methanol or succinate, while putrescine to be the one in nutrient broth-grown cells. The overall content of polyamines in cells growing on glucose was less than that in cells growing on other substrates. Spermine was the most abundant polyamine in glucose-grown cells. Accumulation of polyamines in M. extorquens AM1 was maximal at the mid-exponential or early stationary phase during growth on each substrate. The effect of polyamines added into the medium on the polyamine composition in M. extorquens AM1 was variable. Each polyamine added into the nutrient broth medium was found to increase the amount of the respective polyamine in the cell. Exogeneously added polyamines had no effect on the growth of M. extorquens AM1.
Secretion of the cloned serratia marcescens nuclease in escherichia coli
The Korean Journal of Microbiology, volume 28, issue 4, 1990, Pages 297~303
Secretion of Serratia marcescens nuclease by E. coli harboring pNUC4 was investigated. 29.2, 54.2 and 16.6% of total nuclease were observed in culture medium, periplasm, and cytoplasm of E. coli, respectively. To investigate the secretion mechanism of Serratia nuclease by E. coli, secretion kinetics of nuclease was examined in the presences of sodium azide, and energy metabolism inhibitor; procaine, an exoprotein processing inhibitor; and chloramphenicol, a protein synthesis inhibitor. In the presence of sodium azide, periplasmic unclease was gradually decreased and the extracellular nyclease was linearly increased according to the incubation time. Similar results were obtained in presences of procaine and chloramphenicol. From these results, we concluded that two transport processes are involved in nuclease secretion: secretion of nuclease through the inner membrane is occurred by an energy-dependent process and probably requiring precusor processing: secretion of nuclease through outer membrane does not require energy, de novo protein synthesis, and precursor processing.
Protoplast Formation and Regeneration of Thermophilic Clostridium thermocellum and Clostridium thermohydrosulfuricum
The Korean Journal of Microbiology, volume 28, issue 4, 1990, Pages 304~310
The conditions for protoplasts formation and regeneration of thermophilic anaerobic C. thermocellum and C. thermohydrosulfuricum were determined under the anaerobic growth conditions. The cells of C. thermocellum in initial exponential growth phase were identified to be the most suited for protoplast formation. The optimal conditions for protoplast formation were found to be at
for 2 hours with 0.5 mg/ml of lysozyme in TMG buffer (pH7.5). On the other hand, C. thermohydro-sulfuricum grown in the same medium but excluding glycine was optimally protoplasted at the same conditions but with 0.2 mg/ml of lysozyme. The protoplasts of both strains only subjected to lysozyme treatment of the short time were satisfactorily regenerated after 7-10 days incubation at
in regeneration medium containing 0.3-0.4 M sorbitol, 0.5% casamino acid, and high concentration of
. The regeneration frequencies of the protoplasts of C. thermocellum and C. thermohydrosulfuricum were found to be very low level of
, respectively. The nonregenerated L-form cells were also observed inregeneration medium together with regenerated cells.
Characterization of fusants between thermophilic clostridium thermocellum and clostridium thermohydrosulfuricum
The Korean Journal of Microbiology, volume 28, issue 4, 1990, Pages 311~317
Intraspecific and interspecific protoplast fusions in/between C. thermocellum and C. thermohydrosulfuricum were studied. Protoplast fusions were well induced in 30-40% PEG solution, however, their fusion frequencies were low level of 1.2*10
for intraspecific fusion of C. thermocellum,
for C. thermohydrosulfuricum, and 4.2*10
for interspecific fusion between above two Clostridia, respectively. Most fusants were unstable and segregated after 3 subcultures. Relatively stable intraspecific C. thermocellum fusant FTT17, intraspecific C. thermohydrosulfurecum fusant FSS22 and interspecific fusant FTS3, which were stable after several subcultures, were selected and properties of fusants were further investigated, Phenotypes of the fusants were similar with wild types mostly in cellular morphology, carbon source assimilation and enzyme activities. However they were differed in assimilation of pyruvic acid and sorbitol as carbon source. The DNA contents of fusants were slightly increased compared with wild types. Ethanol production by intraspecific and/or interspecific fusants was not increased, however, acetic acid production as byproduct was decreased or not detected, which indicates that industrial thermophilic anaerobes can be improved by means of protoplast fusion of two strains.
Comparison of Methods for the Measurement of Bacterial Production in a Lake Ecosystem
The Korean Journal of Microbiology, volume 28, issue 4, 1990, Pages 318~323
The bacterial secondary production was measured at 6 sites of Lake Soyang in October, 1989 by
H-thymidine incorporation rate. Verfication for the method of bacterial secondary production measurement showed that
H-thymidine incorporated into DNA, RNA and protein by average percentage of 38.45, 42.27 and 20.07%, respectively. THe more increased incoporated
H-thymidine, the more increasde DNA fraction, but protein fraction was generally low. Incorporation of rate of /usp 3/H-thymidine.
H-leucine into protein correlated with protein fraction of incorporated
H-thymidine. Conversion factors were calculated as follows;
cells/moles of thymidine incorporated/hr and 1.69*10
cells/moles of leucine incorporated/hr.
Distribution and Activity of Heterotrophic Bacterial Communities in Kyeonggi Bay, Korea
The Korean Journal of Microbiology, volume 28, issue 4, 1990, Pages 324~330
The distribution of physicochemical environmental factors and microbiological factors was studied at 6 sampling sites in Kyeongge Bay of Yellow Sea from October 1989 to October 1990. The total bacterial number, saprophytic bacterial number, petroleum-degrading bacterial number, bacterial biomass, and bacterial secondary production were measured in the range of 0.09~1.24*10
cells/ml, 7~60000 CFUs/ml, 0~240 cells/ml, 14.16~301 .
g-C/l, and 0.13~11.82 mg-C/m
/hr, respectively. The turnover times of
H-acetate were in range of 6.5~6984 and 41~24897 hours, respectively. The spatial distribution of heterotrophic bacterial communities were hightly affected by influx of organic pollutants from the coastal area and the seawater exchange with offshore.
Seasonal Distribution and Characteristics of Heterotrophic Marine Bacteria in the Intertidal Zone Near Kunsan of Yellow Sea, Korea
The Korean Journal of Microbiology, volume 28, issue 4, 1990, Pages 331~336
Annual distribution of hiterotrophic marine bacteria and seasonal characteristics were investigated in the intertidal waters and sediments in the vicinity of Kunsan of Yellow Sea, Korea. Annual distribution of heterotrophic marine bacteria ranged from
c.f.u./ml in water and from
c.f.u.per g dry sediments. As for the morphological distribution measured by epifluorescence microscopy, rod-form bacteria were distributed more than 74% of total observed bacteria during surveying periods. Average biovolume of sampled bacteria ranged from
for coccus-form bacteria. Isolated bacteria showed various utilization of carbon sources such as glucose, maltose, lactose, xylose and arabinose, and showed tolerance at various range of salinity. It was isolated 82 genus in seawater and 114 genus in sediments. Dominant genera were Pseudomonas, Vibrio, Flavobacterium and Acinetobacter in seawater, and Pseudomonas, Acinetobacter, Vibrio, and Mycobacterium in sediments.
Filamentous Bacteria Found in Rotating Biological Contactors Treating Domestic Wastewater
The Korean Journal of Microbiology, volume 28, issue 4, 1990, Pages 337~344
Slime sampless from 10 ratating biological contactor (RBC) plants in New Jersey were microscopically examined. Filamentous bacteria such as Type 1701, Type 0041, Type 021N, Nocardia, Beggiatoa, and Sphaerotilus, which are commonly present as suspended forms also were found in RBC slimes growing as attached forms. However, the abundance wwas much different from that of activated sludge. In RBC slimes, Beggiatoa was most frequently observed filamentous bacteria and Sphaerotilus, Type 0041, Type 1701, Type 021N and Nocardia were present in decreasing order of abundance. There were morphologically two different types of Behhiatia in RBC slimes. The statistical anaysis shows that filamentous bacterial poulations between the 100 cm inside and the outside the RBC were different in most cases with significant interactions between the location and stage.
Isolation and properties of D-xylose fermenting yeast
The Korean Journal of Microbiology, volume 28, issue 4, 1990, Pages 345~350
In order to ferment D-xylose directly to ethanol, Yeasts capable of utilizing D-xylose as a sole carbon source and energy source were isolated from soil, sawdust and rotten woods. Among them, the yeast strain, which showed the best ability to produce ethanol, was identified as Candida sp. L-16 isolated from rotten woods. The optimal conditions for production of ethanol were 60rpm of agitation speed, 28j.deg.C of temperature, 4.5 of initial pH and 5% of D-xylose concentration. Ethanol production was reached to maximum state for 4 days culture. Under these optimal conditions, the maximum ethanol concentration and theoretical ethanol yield were 2.4%(v/v) and 74.4% of theoretical value, respectively.
Conversion of Plant Sterols to Androsta-4-ene-3,17-dione by a mutant of Mycobacterium sp. NRRL B-3805
The Korean Journal of Microbiology, volume 28, issue 4, 1990, Pages 351~363
A mutant was selected by NTG treatment of Mycobacterium sp. NRRL B-3805, which was capable of degrading plant sterol to androsta-4-ene-3, 17-dione and yields was higher than NRRL B-3805. Also this mutant produced androst-4-ene-3, 17-dione faster than NRRL B-3805. It described the mode of sitosteroidal degradation, and the interrelation between cell membrane and its attachment to substrate during the sterol degradation process by this mutant and it was compared with Mvcobacterium sp. NRRL B-3805.