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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
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Volume & Issues
Volume 29, Issue 6 - Dec 1991
Volume 29, Issue 5 - Nov 1991
Volume 29, Issue 4 - Sep 1991
Volume 29, Issue 3 - Jul 1991
Volume 29, Issue 2 - May 1991
Volume 29, Issue 1 - Mar 1991
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Secretion of Membrane-Associated Laccase in Liquid Culture of Coprinus congregatus
The Korean Journal of Microbiology, volume 29, issue 5, 1991, Pages 267~269
The hyphal tip laccase of Coprinus congregatus which is a membrane-associated enzyme and shows diffdrdnt banding patterns of PAGE analysis when compared with the enzyme of liquid culture (Choi et al. 1987) has been successfully secreted to culture medium in liquid shake culture by lowering the pH of medium to 4.0. When the fungus is cultivated in YpSs(pH 4.0) liquid, only the hyphal tip laccase is found in the medium after 6 hr incubation and there is no liquid-type enzyme when examined by PAGE analysis.
Intragenic Suppressors for Expory-defective Signal Sequence Mutation of Ribose-binding Protein in Escherichia coli
The Korean Journal of Microbiology, volume 29, issue 5, 1991, Pages 270~277
A mutational alteration in the signal sequence of ribose-binding protein (RBP) of Escherichia coli, rbsB103, completely blocks the export of the protein to the periplasm. Intragenic suppressors for this mutation have been selected on minimal medium with ribose as a sole carbon source. Six suppressor mutations were characterized in detail and were found to have single amino acid wubstitution in the mature portion of RBP, which resulted in the mobility shift of the proteins on SDS polyacrylamide gel. Amino acid changes of these suppressors were localized in several peptides which are packed to form the N terminal domain of typical bilobate conformation of RBP. The involvement of SecB, a molecular chaperone, was investigated in the suppression of signal sequence mutation. Translocation efficency was found to be increased by the presence of SecB for all suppressors. It is likely that the folding characteristics of RBP altered by the suppressor mutations affect the affinity of interaction between SecB and RBP.
Effect of Amino Acid Substitutions of Escherichia Coli
The Korean Journal of Microbiology, volume 29, issue 5, 1991, Pages 278~283
Two amino acid residues (
of E. coli .gamma.-glutamylcysteine synthetase have been investigated whether they are the site of feedback inhibition by site specific mutagenesis. Single substitution of
(S495F), and double substitutions of alanine
(A494G-S495F) resulted in the inactivation of the .gamma.-glutamylcysteine synthetase activity. Substitution of
resulted in a higher level of feedback inhibition. These results suggest that
in .gamma.-glutamylcysteine synthetase is required for its catalytic acitvity and
is presumably related to the feeback inhibition site.
3'-terminal sequence of mucor racemosus 18S rRNA gene
The Korean Journal of Microbiology, volume 29, issue 5, 1991, Pages 284~289
the nucleotide sequence of the 3' terminal 568 bases of the 18S rRNA gene from Mucor racemosus was determined. The 3' end of the structural gene was identified by comparison with the published sequence for the Saccharomyces cerevisiae gene. The M. racemosus gene was found to share 83.8% homology with that of S. cerevisiae and 71-81% homology with those of human, mouse, maize, Xenopus laevis and Tetrahymena thermophila. The known methylation sites in X. laevis and human were also highly conserved in M. racemosus and located within most conserved regions of 18S RNA gene throughout evolution.
The DNA region of rtn gene essential for resistance against N4 infection
The Korean Journal of Microbiology, volume 29, issue 5, 1991, Pages 290~295
N4 phage, which infects E. coli K-12 strains, could not infect E. coli K-12 strains containing rtn(resistant to N4) gene on plasmids, which was isolated from Proteus vulgaris ATCC 13315. The region of rtn gene for Rtn phenotype was reduced to the 1.7 kb HincII-AccI fragment, and rtn gene seemed to have its own promoter. This putative promoter was present in 107 bp HindII-DraI fragment, and known to be functional in E. cole K-12, which is supported by the fact that phenotype of a subclone, pRMG103A1B which does not contain the 107 bp fragment, was dependent on the existance of a functional promoter in the upstream of rtn gene, and that the 107 bp fragment had promoter activity when located in the upstream of structural gene of galactodinase of E. coli. The promoter-bearing fragment contains two overlapping putative promoter sequences, both of which show a fit in eight of twelve nucleotides with consensus sequences of E. coli promoters at the -35 and -10 regions.
Identification and Partial Purification of Two Hydrogenase Isoenzymes from Escherichia coli
The Korean Journal of Microbiology, volume 29, issue 5, 1991, Pages 296~300
The membrane-bound Escherichia coli hydrogenases were purified partially by the solubilization with detergents. the E. coli crude extract was solubilized with sodium deoxycholate and dialyzed against the buffer containing Triton X-100. Two different hydrogenases were obtained by the DEAE-cellulose, hydroxyapatite and Sephedex G-200 column chromatography. The one was unstable during purification and contained 70- and 47-kDa polypeptides as major proteins. The other showed high H2-evolving activity and had major polypeptides of Mr 31 and 27. Those polypeptides were detected by the two-dimensional electrophoresis.
Characterization of extracellular fructosyl transferase from aureobasidium pullulans C-23
The Korean Journal of Microbiology, volume 29, issue 5, 1991, Pages 301~306
Extracellular fructosyl transferase from Aureobasidium pullulans C-23 was characterized. The molecular weight of the isolated enzyme was determined to be approximately 170,000 by SDS polyacrylamide gel electrophoresis. The enzyme has the pI value of about 3.7. The enzyme was almost completely inhibited by 5mM
, but was not significantly affected by other cations tested. The enzyme was inactivated by treatment of tryptophan-specific reagent N-bromo- succinimide and tyrosine-specific reagent iodine. The substrate sucrose showed protective effect on the inactivation of the enzyme by the both reagents. These results suggest that tryptophan and tyrosine residues are probably located at or near active site of the enzyme.
Biochemical studies of the siderophore A3 produced by pseudomonas synxantha A3
The Korean Journal of Microbiology, volume 29, issue 5, 1991, Pages 307~313
A yellow-green, fluorescent siderophore A3 was extracellularly produced under iron-limited growth conditions from Pseudomonas synxantha A3. The physicochemical and biological properties of siderophore A3 were examined. The approxiamte molecular weights of the Fe(III)-siderophore A3-1 complex and Fe(III)-siderophore A3-2 complex were estimated to be about 1,300 and 1,100, respectively, by Bio-gel P2 gel exclusion chromatography. The molar ratio between the siderophore and the Fe(III)was 1.08 mole. The molecular weight of the complex could be calculated with this ratio and the new values were 1,150 and 960, respectively. The binding constant(K) between thesiderophore A3 and Fe(III) that determined by displacing the iron from the Fe(III)-siderophore complex with EDTA was 4.12*10
at pH 5.0. Siderophore A3 appeared to have antibacterial activity on several bacterial strains, however, ferric siderophore Ae complex did not show that activity. The cytotoxicity of siderophore A3 was obtained from Human Chronic Myelogenous Leudemia K562 cells. Inhibition concentration (50%)(
Effect of Triton X-100 on the Growth and Morphology of Trichoderma koningii
The Korean Journal of Microbiology, volume 29, issue 5, 1991, Pages 314~318
We investigated the effect of Triton X-100 on the growth and morphology of Trichoderma koningii by comparing various parameters representing the frowth of mold in the presence or absence of Triton X-100. The specific growth rate and doubling time of T. koningii were not affected by the addition of 0.05% Triton X-100 in batch culture. However, in the presence of Triton X-100, cultures reached its stationary phase earlier and showed reduced level in total yield of biomass. The addition of Triton X-100 into solid medium also resulted in decrease in the colony radial growth rate and this response was correlated with the formation of mycelia which showed increase in branching and septation in the presence of Triton X-100.
Isolation of vibrio species from cultured flounders (paralichthys olivaceus) with uicers and ascites in the southern coast of Korea during the winter season
The Korean Journal of Microbiology, volume 29, issue 5, 1991, Pages 319~328
this study was performed for the purpose of surveying the distribution and characteristics of the Vibrio species in the diseased farm flounders (Paralichthys olivaceus) during the winter season in Korea. Samples were collected along the southern coast in the Sinam, Keoje Island, and Teosu areas from November '90 to Feburary '91. Nine species of Vibrio were identified as V. tubiashii (85 strains), V. damsela (78 strains), V. anguillarum (21 strains), V. compbelli (9 strains), V. fluvialis (9 strain), V. costicola (1 strain), V. alginolyticus (1 strain), V. gazogenes (1 strain), V. marinus (1 strain), and unidentified 3 groups of bacteria (154 strains) which may have related with V. tubiashii were isolated. Eleven kinds of discs (BBL Co.) were used for antibiotic susceptibility test and 116 strains were selected for the experiment. Most bacteria were powerfully inhibited their growth by 4 antibiotics such as cephalothin, chloramphenicol, colistin, and tetracycline, but they were poorly inhibited by 4 antibiotics such as carbenicillin, kanamycin, penicillin G, and streptomycin.
The Environmental Factors Affecting the Distribution and Activity of Bacteria in the Estuary of Naktong River
The Korean Journal of Microbiology, volume 29, issue 5, 1991, Pages 329~338
From July 1985 to December 1986, 28 variables of phycal-chemical factors, bacteria and heterotrophic activity were investigated 17 times at 3 stations in the estuary of Naktong River and the influences of environmental factors to bacterial population and heterotrophic activity were analyzed through multiple regression. The results of multiple regression were as follows. At station 1, total bacteria and heterotrophic bacteria(Z-25) could explain 57% of the variation of maximum uptake velocity for glucose and 54% of turnover time for glucose was explained by total coliform bacteria and MBOD, Sixty four percent of the variation of Kt+SN was accounted for salinity, MBOD-N and inorganic phosphate. Turnover rate for acetate was also accounted for the change of MBOD-P by 56%. At station 2 maximum uptake velocity for glucose depends on MBOD-N by 81%; turnover time on bacteria by 50%; Kt+Sn on avilable nutrient by 61%. More than 50% of maximum uptake velocity and turnover time for glucose were influenced by bacteria and that of Kt+Sn by the change of nutrient in the surface water of station 3. In the bottom water of station 3, the change of maximumuptake velocity, turnover time and Kt+Sn for glucose was controlled by total bacteria and available nutrient, bacteria, the change of nutrient salts respectively. On the whole, more than 50% of maximum uptake velocity and turnover time for glucose could be due to the change in the number of bacetria and the value of Kt+Sn was affected by the change of nutrient salts. Turnover rate for acetate was controlled by available phosphate at station 1 and by bacteria at station 2 and 3, which showed a distinct difference between the environmental factors which govern the rate of glucose and acetate uptake in the Naktong esturine ecosystem. And bacterial communities were controlled by available nutrients at station 1, by nutrient salts and salinity at station 2 and in the surface water of station 3 and by salinity in the bottom water of station 3.
Seasonal Variation of Heterotrophic Activity in the Estuary of Naktong River over Half Tidal Cycle and Salinity Effect
The Korean Journal of Microbiology, volume 29, issue 5, 1991, Pages 339~343
Heterotrophic activity, total bacteria and salinity were determined seasonally in the estuary of Naktong River over half tidal cycle. Heterotrophic activity was determined by the uptake of [U-
C]glucose. Heterotrophic activity fluctuated with the tides and was decreased as salinity increased. Teh great activity occurred near low ebb tide at all seasons except summer. The main environmental factor affecting hetreotrophic activity was the salinity rather than water temperature in the estuary of Naktong River. In order to estimate the effect of salt, salt was added to estuarine water. Vmax for glucose of salt-added water was 17% and 77% of original estuarine water at station 1 and 2 respectively and slight increase was observed at station 3. Respiration rate and Kt+Sn for glucose of salt-added sample increased at all 3 stations. The increase of the Kt value implies the reduced affinity of bacterial population for glucose. The effects of salinity on the heterotrophic activity were more extensive in the upper region of estuary than at the mouth.