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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 29, Issue 6 - Dec 1991
Volume 29, Issue 5 - Nov 1991
Volume 29, Issue 4 - Sep 1991
Volume 29, Issue 3 - Jul 1991
Volume 29, Issue 2 - May 1991
Volume 29, Issue 1 - Mar 1991
Selecting the target year
Purification and Characterization of Extracellular Proteinase Produced by Pseudomonas aeruginosa
The Korean Journal of Microbiology, volume 29, issue 6, 1991, Pages 345~352
A serine proteinase of molecular weight 60 kd was purified from culture supernatant of P. aeruginosa using DEAE-Trisacryl M ion-exchange and AcA 54 gel filtration column chromatography, and the properties of serine proteinase were characterized. By means of SDS-polyacrylamide gel electrophoresis, the molecular weight of the enzyme was 55 kd. The optimal pH for the activity of purified enzyme was 7.5. The activity of the purified enzyme was completely inhibited by Di-isopropylfluorophosphate(DFP) and N-.alpha.-p-tosyl-L-lysine choloromethyl detone(TLCK) but not by other proteinase inhibitors such as E-64, pepstatin A, 1, 10-phenanthroline. The purified enzyme was capable of degrading type I and type IV collagen. Antisera obtained from hymans infected with Pseudomonas aeruginosa reacted to the purified serine proteinase in immunoblots. These results indicate that the purified enzyme is trypsin-like serine proteinase and this enzyme of P. aeruginosa may play an important role in tissue damage as a spreading factor and may be useful for serodiagnosis of Pseudomonas infections.
A study on the Synthesis and Biological Activity of Nucleoside Chemotherapeutic Agents
The Korean Journal of Microbiology, volume 29, issue 6, 1991, Pages 353~360
5-substituted uridine(I,Br,Cl), 5'-amino-5'-deoxyuridine conjugates of amino acid, peptide and penicillin G, 5'-monophosphate uridine derivatives and 5'-monophosphate-fatty acid detrivatives were chemically synthesized. Their biological activities were determined as MIC and IC/sub 50/ unit against various pathogenic microorganisms in vitro. 5'-amino-5'-deoxyuridine-cyclo(Phe-Asp)(23), 5-iodo-5'-amino-deoxyuridine-penicillin G(26) were the most efficient; their IC/sub 50/ against L5178Y murine lymphoma cell was 6.5 h/ml, MIC against S. aureus (+) and E. coli (-) was 6.25 g/ml. MIC of 5-bromo-2', 3'-O-isopropylideneuridine(6) against Trichophyton rubrum was 0.2 g/ml. And 5'-monophosphate derivatives are more active than simple uridine derivatives, suggesting other modified nucleoside 5'-phosphate may be worthwhile examing further as a new prodrugs.
Effects of environmental factors on the outbreak of freshwater red tide by peridinium bipes in Soyang reservoir
The Korean Journal of Microbiology, volume 29, issue 6, 1991, Pages 361~370
Physical and chemical environmental factors influencing on the outbreak of freshwater red tide by Peridinium bipes (dinoflagellate) in Soyang Reservoir were studied. Red tide occured in the site of inflowing of tributary streams annually, but the extent and severity of red tide varied from year to year. Several environmental factors such as water level, nutrient releasing from sediment, cyst resuspension, and concentrations of
were studiedin relation to development, extent, and duration of red tide. In June of 1989 and 1991, the red tides of Peridinium bipes were very severe, and these red tides coincided with notable and rapid drawdown of lake water in late spring. Nutrient releasing and cyst resuspension by turbulence during drawdown were suggested as main causes of red tide. The quanity of nutrient releasing from sediment and hydrometeorological factors such as run-off and wind may determine the extent and duration of red tide.
Effects of Freshwater Red Tide by Peridinium bipes on Microbial Loop in the Water Column of Soyang Reservoir
The Korean Journal of Microbiology, volume 29, issue 6, 1991, Pages 371~379
Effects of freshwater red tide of Peridinium bipes (dinoflagellate) in August of 1991 on the carbon flux through the microbial loop were studied in Soyang Reservoir. Chlorophyll a and primary production, bacterial production in red tide area were 22, 18 and 400 times higher, respectively, than other sites. Phytoplandton biomass comprised 97% and 20% of food source of zooplankton grazing within and without red tide, respectively. The percent bacterial production supported by phytoplankton exudate was 14% within red tide and >100% without red tide. In laboratory experiments, more than 85% organic carbon of Peridinium biomass was released or degraded by heterotrophic bacteria within 14 days. As results of red tide of Peridinium with sudden influx of organic carbon in water column, the main food source of zooplankton and dependency of bacteria on phytoplankton exudate were changed. Therefore, the relative importance of microbial loop to grazing food web was changed.
Purification and Some Properties of Soluble Cytochrome c from Methylobacillus sp. Strain SK1
The Korean Journal of Microbiology, volume 29, issue 6, 1991, Pages 380~386
Three types of soluble cytochrome c were purified to homogeneity from Methylobacillus sp. strain SK1 which grows only on methanol. Cytochrome c-I was purified 58.5-fold in seven steps. Cytochrome c-II and c-III were purified 57.3- and 122.1-fold in eight steps, respectively. The molecular weights of the cytochrome c-I was determined to be 12,500, while those of the cytochrome c-II and c-III were 16,000. The isoelectric points of the c-I, c-II and c-III were found to be 8.8, 6.6, and 6.6 respectively. The spectrum of reduced cytochrome c-I showed .alpha.-, .betha.-, .gamma.-peaks at 551.4, 522.2, and 416.6nm. The peaks for c-II were found at 551.0, 521.6, and 416.5nm, while those for c-III were shown at 551.2, 521.8, and 416.0 nm. The spectra of oxidized cytochrome c-I, c-II, and c-III showed .gamma.-peak at 411.8, 409.0, and 410.2 nm, respectively. The absorption coefficients of .alpha.- and .gamma.-peak for c-I in the reduced state were determined as 47 and 197
, respectively. The coefficients of .alpha.- and .gamma.-peak for c-II were determined to be 43 and 137
, while those for c-III were 41 and 172
, respectively. The c-I and c-III were found to bind carbon monoxide.
Effects of Cultivation Conditions on the Growth and Polyamine Composition in Methylobacterium extorquens AM1 Growing on Methanol
The Korean Journal of Microbiology, volume 29, issue 6, 1991, Pages 387~391
Methylobacterium extorquens AM1 growing on methanol as a sole source of carbon and energy was found to grow most rapidly (t
=6h) at 30.deg.C in a mineral medium (pH 7.0) containing 0.5% (v/v) methanol which was agitated at 200 rpm (optimal cultivation condition). Cells grown under the optimal cultivation condition contained more spermidine, but less putrescine, than the cells grown on 2.5%(v/v) (
=8h ) or at 20.deg.C (
=8h ). Cells cultivated under the optimal condition was found to contain more spermidine than the cells grown at pH 6.0 ((
=7h ) and pH 8.0 (
=7.3h). the cells growing at the stationary phase under the optimal condition accumulated more spermine or putrescine than the cells growing at the same phase on 2.5%(v/v) methanol or at pH 6.0 and pH 8.0, respectively. M. extorquens AM1 grown in a medium agitated at 100 rpm (
=8.8h ) contained less spermidine and spermine than the cells grown under the optimal cultivation condition.
Characterization of the din (damage-inducible) and tin (temperature-inducible) Genes Isolated from Escherichia coli
The Korean Journal of Microbiology, volume 29, issue 6, 1991, Pages 392~396
Mu d1(Ap lac) bacteriophage can be used to search for genes which are members of a common regulatory network without having to know the functions of the genes in advance. Aim was for obtaining the loci in the SOS network as well as temperature inducible loci. For this purpose, recA441 allele was used. This allele encodes a thermosensitive recA gene product; thus, the recA441 allele can be activated upon temperature upshift without by external DNA damage. Approximately 10, 000 colonies were screened, and then searched for the colonies which expressed .betha.-galactosidase higher level at 42.deg.C than at 30.deg.C. The strains identified fell into two dlasses; (i) ones in which the increased expression was
-dependent, that is, din(damage-inducible) genes which were due to the activation of recA441 allele and (ii) ones in which the increased expression was
-independent and only temperature-inducible, tin genes. Rough mapping position was obtained for these genes.
Morphological Changes of Hansenula anomala B-7 by Cadmium Ion
The Korean Journal of Microbiology, volume 29, issue 6, 1991, Pages 397~401
Yeast-form cells of cadmium ion-tolerant Hansenula anomala B-7 were changed to mycelial cells in medium containing more than
g/ml of cadmium. Moreover, the mycelial cells were exchanged into clumped cells in a medium containing more than
g/ml of cadmium. Optimal conditions of mycelial cell formation were achieved in the presence of .
g/ml of cadmium with shaking cultivation for 7 days. Glucan and mannan contents of the yeast cell wall frown with
g/ml of cadmium decreased by 10% compared with those grown without cadmium. However, protein and lipid contents increased about 20% respectively. By cadmium, no significant findings in specific amino acid contents were discovered.
Determination of Marine Bacterial Number and Biovolume in the Intertidal Zone of the Yellow Sea near Kunsan, Korea
The Korean Journal of Microbiology, volume 29, issue 6, 1991, Pages 402~407
As a baseline survey to clarify the structure and function of bacterial populations in the intertidal waters of the Yellow Sea near Kunsan in 1987/1988, annual variation of bacterial number and biovolume was estimated by AODC method. Rod-shaped bacteria were more than 74% of all cells during the investigation period. Average biovolume of sampled bacteria ranged from
for coccoid bacteria, and from 4.57.+-.0.
for rod-shaped ones. It showed that larger biovolume occurred at surface in case of coccoid bacteria, while at bottom in case of rod-shaped ones. There was little correlation between population density and biovolume. Diameters of edlls were below
m among 77-89% of all measured coccoid bacteria, and lengthes of cells more than 70% of all measured rod-shaped ones were below
m. It explains the high percentage of "mini-bacteria" was distributed in the investigation area.tion area.
production of photosynthetic bacteria transferred TOL plasmid from flavobacterium odoratum
The Korean Journal of Microbiology, volume 29, issue 6, 1991, Pages 408~415
TOL plsmid size of Flavobacterium odoratum SUB53 was estimated as 83 Md and the optimum concentration of m-toluate degradation by TOL plasmid was 5 mM.
production by Rhodopseudomonas sphaeroides KCTC1425 was largely dependent on nitrogenase activity and showed the highest at 30 mM malate with 7 mM glutamate as nitrogen source. Nitrogenase activities were inhibited by 0.3 mM
ions, to be appeared the decrease of
production. Conjugation of TOL plasmids from F. odoratum SUB53 and Pseudomonas putida mt-2 to R. sphaeroides showed the optimum at the exponential stage of recipient cells in presence of helper plasmid pRK2013. According to the investigation of catechol-1,2-oxygenase (C-1, 2-O) and catechol-2,3-oxygenase (C-2,3-O) activities of R. sphaeroides C1 (TOL SUB53) and C2 (TOL mt-2), the gene for C-2,3-O is located on TOL plasmid and gene for C-1, 2-O on the chromosome of R. sphaeroides. m-Toluate was biodegraded by TOL plasmid in R. sphaeroides C1 and C2, presumably to be produced
gas from the secondary metabolites of m-toluate.e.