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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 3, Issue 2 - Oct 1965
Volume 3, Issue 1 - Apr 1965
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Studies on potato virus disease in Korea
The Korean Journal of Microbiology, volume 3, issue 2, 1965, Pages 1~8
The potato viruses, as possible potato virus X(PVX), potato virus Y(PVY), potato virus S(PVS) are isolated from potato tuber, which collected from eleven areas (Table 1) in Korea. These viruses are isolated by single lesion isolation, Aphid transmission and inoculating methods through the many species of the different plants. The PVX is identified by host range, symptoms, physical properties, serological reaction and electron micrography. The other two viruses are identified by the first two methods mentioned above. The results of the above experiments are as follows. The total value of these viruses infection is 81%. The value of PVX infection is higher than the other two viruses. The properties of PVX are marked as local lesions on Comphrena globosa. The dilution end point is
, the thermal inactivation point is
and the size of virus particles is around 13 x 60
Studies on Koji for Soy Sauce Brewing (Part. 3)
The Korean Journal of Microbiology, volume 3, issue 2, 1965, Pages 9~14
The enzyme-producing potentials of industrially important strains of Aspergillus spp. were studied. Irradiation of three original isolates of Aspergillus oryzae to ultra-violet rays resulted in the production of mutants which differed from the parent riboflavin and vitamin
in culture media. 1. Irradition three strains of Aspergillus oryzae to ultraviolet light produced mutants and two strains of them were selected for soy sauce brewing. 2. The two strains are the physiological mutants of Aspergillus oryzae. Both were found to have superior enzyme activity to their relatives. 3. Aspergillus oryzae UV-induced mutant 172-722 and 569-713 were more powerful than others in the production of riboflavin and vitamin
. The enzyme activity of these strain were high and decreased only slightly even in 20 percent solution of NaCl. 4. Aspergillus oryzae UV-induced mutant 172-722 had more powerful protease producibility in wheat bran media than in modified Czapek's solution. On the contrary, Aspergillus oryzae UV-induced mutant 569-713 had more powerful producibility of saccharogenic and dextrinogenic amylase in modified Czapek's solution than in mold bran. 5. Aspergillus oryzae UV-induced mutant 172-722 formed the spore rapidly and Aspergillus oryzae UV-induced mutant 569-713 did ordinarily. 6. It is found from the results that Aspergillus oryzae UV-induced mutant 172-722 is valuable material for the manufacture of soy sauce because of its high protease activity in 20 percent solution of NaCl. Aspergillus oryzae UV-induced mutant 569-713 is suitable for soy bean mash and for fermented red pepper sauce for its high saccharogenic and dextrinogenic amylase activity in 20 percent solution of sodium chloride.
Studies on the organic acids metabolism in chlorella cells.
The Korean Journal of Microbiology, volume 3, issue 2, 1965, Pages 15~21
Using the synchronous culture method and the manometric technique, changes in respiratory activities, utilization of some organic acids (succinate, malate, lactate and acetate etc.) and its effect on glucose metabolism in Chlorella cells at different growing stages were measured. 1) Endogenous respiration of the cells was not active at growing stage and was almost constant throughout the early ripening, maturing and division stages. 2) Lactate was utilized as respiratory substrate better than other organic acids tested. Exogenous respiration of glucose was most active at growing and maturing stages and was decreased strikingly at division stage. 3) Succinate and citrate inhibited endogenous and glucose respiration of the cells throughout the all life cycle. 4) Malate and acetate were utilized in the cells at early growing and division stages better, and malate enhanced the glucose respiration while in case of acetate it was depressed. 5) Calcium ion inhibited not only permeability of respiratory substrate but endogenous respiration itself.
Mosaic Disease of Black Locust on Rodinia pseudo-acacja L.(Par. 2)
The Korean Journal of Microbiology, volume 3, issue 2, 1965, Pages 22~26
In order to investigate the host range of the mosaic disease of black locust in the Chunchon area, the sap of the mosaic-diseased leaves of black locust itself and the cowpea leaves infected with the above mentioned sap, were inoculated to 53 species of plants belong to 12 families. As to the result, no difference in infection was found as related to the virus sources, and the infection was recognized in 4 species of the family Chenopodiaceae and 8 species of the family Leguminosae. The plants recognized as hosts are as follows: the plants which showed local infection are Chenopodium album, Ch. ambrosioides, Ch. quinoa; the plants which showed systemic infection are Chenopodium amaranticolor, Phaseolus vulgaris, Robinia pseudo-acacia, Vigna sinensis; and Astragalus sinicus, Melilotus indicus, Phaseolus angularis, Pisum sativum and Vicia faba were recognized as carriers. Through investigating its host ranges and symptoms, this mosaic virus of black locust seems not to be regarded as the group of the black locust mosaic virus in southeastern Europe reported by Milinko et al (1961). And, too, it is thought hardly to exist in combination with the cowpea mosaic virus. It appears, therefore, that this mosaic virus was confined to that of black locust.
Ueber die chlorophyllbildung von Chlorella variegata bei Glukose-anzucht
The Korean Journal of Microbiology, volume 3, issue 2, 1965, Pages 27~43
Wir haben das Wachstum und Chloropbyllzunahme und -abnahme bei der Autotroph-, Mixotroph-, und Heterotrophanzucht analysiert, und ob Glukose-Chlorose von Stickstoffi-nangel verursacht wird, oder Glukose die Chlorophyllbildung unterdriickt, und wie Lipid und Stdrke gebildet worden ist. Wir wollten bei Glukose-Chlorose Zucker, Stickstoff und Chlorophyll drei Verhiiltnisse untersuchen. Chlorella variegate fehlte Nitrat-Reduktase. Sie wachste nicht mit
als stickstaffquelle und bildete kein chlorophyll bei Heterotroph und unterdrilckte chlorophyllbildung bei Mixotroph. Akkumulation von Lipid und Stdrke war nicht so viel wie bei Mineralsalz mangelchlorose. Chlorella variegate brauchte nicht
die Oxydationsphosphorylierung aber
ist notig. Durch DNP und Fluoracetat blockierte Chlorella variegate die oxydationsph-osphorylieriing. Zell-Vermehrung und Koblenhydratbildung sind reduziert worden, aber nicht normal.