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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Microbiology
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Journal DOI :
The Microbiological Society of Korea
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Volume & Issues
Volume 30, Issue 6 - Dec 1992
Volume 30, Issue 5 - Oct 1992
Volume 30, Issue 4 - Aug 1992
Volume 30, Issue 3 - Jun 1992
Volume 30, Issue 2 - Apr 1992
Volume 30, Issue 1 - Feb 1992
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Molecular Cloning of a Gene Cluster for Phenanthrene Degradation from Pseudomonas sp. Strain DJ77 and Its Expression in Escherichia coli
The Korean Journal of Microbiology, volume 30, issue 1, 1992, Pages 1~7
We cloned a gene cluster encoding phenanthrene-degrading enzymes on a 6.8-kb Xhol fragment from the Pseudomonas sp. DJ77 chromosomal DNA into the vector pBLUESCRIPT SIC(+). The resultant clone, containing the recombinant plilsmid pHENX7, was able to convert 3-methylcatechol to a yellow mela-cleavage compound. Since the pHENX7R in which the DNA insert was cloned in the opposite orientation lacked extradiol dioxygenase activity. the direction of transcription was established. Four polypeptides, PhnC (24 kDa). PhnD (31 kDa), PhnE (34 kDa). and PhnF (15 kDa), were identified in E coli JM101 transformed with several pHENX7-derived plasmids. The locations and extents of ~ndividual genes were determined by subcloning. The gene order was phnC-phnD-phnE-phnF-phnG, and phnC, phnD, phnE, and phnG genes encoded glutathione S-transferase, mrta-cleavage compound hydrolase, extradiol dioxygenase, mera-cleavage compound dehydrogenase, respectively.
Pseudomonas sp. strain DJ77 균주에서 extradiol dioxygenase 를 암호화하고 있는 phnE 유전자의 염기배열
The Korean Journal of Microbiology, volume 30, issue 1, 1992, Pages 8~14
Nucleotide Sequence of phnE Gene Encoding Extradiol Dioxygenase fromPseudomonas sp. Strain DJ77Kim, Young-Chang'.", Myeong-Su Shin1, Kil-Sang Younl, Young-Soon Park1, andUg-Hyeon Kim'.' (Department of Microbiology, C'hungbuk National University.Cheongju 360-763, KOREA. and 'Research Center for Molecular Microbiology,Seoul National University)nal University)
국내 연근해 및 환자로부터 분리된 vibrio vulnificus의 세균학적 특징
The Korean Journal of Microbiology, volume 30, issue 1, 1992, Pages 15~29
Vibrio vuln$cus has been recognized as a pathogen of septicemia and wound infection, when the organism attacks high-risk persons with a history of hepatic disease. alcohol abuse. diabetes or any debilitative disease. Forty six strains of K vulnzjicus. isolated from 1025 marine specimens from May to Novemver for three years. from 1985 to 1987. were studied for their biochemical properties. growth requirements, serotype and drug susceptibilities. The isolates were different in their various biochemical reactions. Ninety-five percent of isolated strains were able to ferment lactose, while most strains didn't utilize sucrose in their biochemical test, for example ornithine, gelatin and mannitol were quite dit'ferent composition than those described in other reports. It was found that the biochemical test wasn't useful for identifying strain. The type of somatic 0 antiserum was determined in isolates from marine sources and in patients with Vibrio septicemia. In patient isolates. 1-2 group were 24% and 1-4 group were 42%. However. 02 group(33%) were more abundant in isolates from marine sources. Minimal inhibitory concentrations(M1Cs) of chloramphenicol, tetracycline. erythromycin and ampicillin were determinef for V vuln~ficus by broth dilution method. MIC90 was I , 0.25, :! and 4,ug/ml in patient isolates. 1, 0.25, 2 and 2 ,ug/ml in marine isolates. The divalent chelating agent, IDTA. inhibited the growth of V. vuln!'ficus at 6.25 mMlml of MIC90.
Frankia sp. strain SNU 014201의 nif-H, D, K, 유전자 클로닝
The Korean Journal of Microbiology, volume 30, issue 1, 1992, Pages 30~36
nif (nitrogen fixation)-H.D, K genes of Frankia sp. SNU 014201. a symbiotic strain isolated from root nodule of Alnus hirsura, were found to be located in the genome on 13.5 kb of EcoRI, 18.0 kb of BamHI, 10.5 kb of BglII and 4.5 kb of KpnI fragments. Using EMBL-3 BamHI arms of bacteriophage lambda. the genomic library was constructed. from which fourteen recombinant phage nif-clones were selected. Among them, Ahnif-I2 had insert DNA of 18 kb, in which 7.9 kb of BamHl fragment contained nif-H, D, K and 3.6 kb of HindlIl/KpnI had nif-H and partial -D. Therefore, the 7.9 kb and 3.6 kb fragments were subcloned and partial restriction maps were constructed. As the results, nif-F1, D.K genes were found to be located continuously on the 6.5 kb of HindII/BamHI and 5.2 kb of SalIIBamHI fragment in the genome of Frankia sp. SNU 014201.
Studies on KEM1 Gene Controlling Mitotic Cell Division in Yeast: Molecular Cloning of a High Copy Suppressor (ROK1) of kem1
Kim, Sang Hyeon ; Kim, Jin Mi ;
The Korean Journal of Microbiology, volume 30, issue 1, 1992, Pages 37~41
The KEM1 gene is known to affect microtubule and spindle pole body function during the cell division cycle in Saccharomjyces cerevisiae. To identify new genes with functions similar or related to those of KEM1, we isolated a high copy suppressor gene (ROK1) that suppresses the kem1 mutation when cloned on a high copy number plasmid but not on a low copy number plasmid. Two clones which suppress both the benomyl hypersensitivity and the
enhancing phenotype of kem1 null mutation were isolated and were shown to have a 9.0 kb identical insert by restriction endonuclease analysis. The restriction map constructed indicates that this suppressor gene, ROK1 is not KEM1. Subcloning experiments suggest that the functional region of ROK1 is at least 3.0kb in size.
Glyphosate 저항성 pseudomonas sp. strain HG-1 의 분리 및 저항성 유전자의 클로닝
The Korean Journal of Microbiology, volume 30, issue 1, 1992, Pages 42~46
From the soil environment which is treated with herbicides. we isolated strain having high resistance against glyphosate. After being identified. the isolated strain was turned out to be Pseudomc~nu.c~c ~paciua nd to have intense tolerance lo the 10 mM glyphosate. The isolated strain shows slow, growth rate about twenty hours in glyphosate comparing with that in inorganic phosphate. As a result of confirming the p~~sitioonf glyphosate resistant gene. it was proved to exist in chromosome. After cloning it into E coli C600, transformants E. coli THC-101 and plasmid pGR19 were obtained.
토양에서 분리한 pseudomonas sp. 에 의한 phosphinothricin 과 glyphosate의 생분해
The Korean Journal of Microbiology, volume 30, issue 1, 1992, Pages 47~52
This study describes isolation and identification of a soil bacterium which is degradable of phosphinothricin and improvement of the isolated strain by using mutagenesis and spheroplast fusion. The experiment was performed to search for a possibility of development of a new strain which is both PPT-degradable and glyphosate-resistant by using interspecies cell fusion between the PPT-degrading bacterium. Pseudornonu.\ puucimohlis and a glyphosate -resistant strain, Pseudornonu.~ cc,pucicl. Auxotrophic mutants were obtained by the treatement of P. puucimohili.\ with ethylmethanosulfate, and used to cell fusion. Lysozyme and EDTA were used to spheroplast formation and regeneration rates :)f the spheroplast were 6.5'%1 in P. pauc.irnohili.\ and 8.8% in P.ci,j~u[,i(lr, espctively. Polyethylenglycol 5.000 was used to cell fusion as fusogen. The fusant\ulcorner F1. F2. F\ulcorner and F4 werc- obtained by the intra- and interspecies cell fusion. The fusant Fl of intraspecies cell fusion was higher to the wild type by 1 I'%l in PPT degrading ability, and the fusant F3 of inierspesis cell fusion developed plyphosatc-resistant and PPI-dcgrading ability which were propertics of two parental strains.
Divergence of the cbp Genes in 4-Chlorobiphenyl Catabolizing Bacteria
The Korean Journal of Microbiology, volume 30, issue 1, 1992, Pages 53~59
Four bacterial strains capable of catabolizing 4-chlorobiphen!;l (4CB) were isolated from the industrial waste waters. The bacterial isolates designated as PO$. P20, P27, and P1242. respectively, were examined for their catabolic activities. And in order to examine molecular homology of the 4CB catabolizing genes of these bacterial isolates. Southern hybridization was conducted with bphABC genes of P. p.srudoalculigrnrs KF707 as a DNA probe. The metabolites of 2-hydroxy-6-0x0-6-(4'-chlorophenyl)hexa-2 .4-dienoic acid and Cchlorobenzoate were detected to be produced by the isolatc:~ in the MM2 liquid cultures. But Cchlorobenzoate was further catabolized to produce 4.-hydroxybenzoate by DJ-12, P08. and P27. but not by P20 and P1242. As results of hybridization, homologous regions were commonly observed in Xhol fragments of 2.2 and 1.8 kb and in EcoRl fragment of 11 kb in the DJ- 12. P08, and P27 isolates. But in any restriction enzyme digests ot the P20 and PI242 isolates. homologous region was not detected. The cbp genes of the bactcria capable of catabolizing 4CB in nature could be divided into two groups by divergence<
Secretion of escherichia coli
-lactamase from bacillus subtilis with the aid of usufully constructed secretion vector
Park, Geon-Tae ; Rho, Hyun-Mo ;
The Korean Journal of Microbiology, volume 30, issue 1, 1992, Pages 60~64
The secretion vector with promoter and signal sequence region of neutral protease gene (npr) from Bacillus amyloliquefaciens was constructed by the technique of polymerase chain reaction (PCR). A unique restriction iste was introduced into the 3' of the signal coding region by the synthesis of PCR primer. To demonstrate the function of cloned promoter and signal sequence, we used the E. coli .betha.-lactamase structural gene as a foreign gene. The signal sequence of .betha.-lactamase gene was deleted by Bal31 exonuclease and only mature region was introduced into the secretion vector. Bacillus subtilis cells transformed by the recombinant vector synthesized the fusion protein and were also capable of removing the signal peptide from the original fusion protein, as judged by the assay of .betha.-lactamase activity and secretion into the growth medium by western blotting.
The Introduction of Polycrylamide Gel into the Solid Culture of Streptomyces spp
Han, Hong-Ui ; Yang, Moon ;
The Korean Journal of Microbiology, volume 30, issue 1, 1992, Pages 65~69
It is proposed the polyacrylamide gel, instead of agar, could be used for the solid cultures of microorganisms including Streptomyces strains. Polymerization and gellation of 5% acrylamide solution were done by autoclaving for 5 min at 121.deg.C and no hindered by the addition of nutrient-rich media. In particular, pH buffer solution suitable for corresponding microorganisms must be used in the preparation of culture media. Comparing with agar, it was discussed that polycrylamide gel had many advantages such as gellation within the wide range of strong acid Carbon and Nitrogen sources, requirement tests of growth factors and minerals, sterization at high temperature, diffusion assays of products depending on the pore size of gel, and stability and standarization of microbial cultures.