Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 31, Issue 4 - Aug 1993
Volume 31, Issue 3 - Jun 1993
Volume 31, Issue 2 - Apr 1993
Volume 31, Issue 1 - Feb 1993
Selecting the target year
Formation of Intergeneric Hybrids Between Aspergillus niger and Penicillium verruculosum by Nuclear Transfer
The Korean Journal of Microbiology, volume 31, issue 1, 1993, Pages 1~8
lntergeneric hybrids formed between Aspergillus niger and Penicillium verruculosum were obtained by nuclear transfer technique. Nuclei isolated from wild type and auxotrophic mutants of donor strains were transferred into the protoplasts of different auxotrophic mutants as recipient strains. Several auxotrophic mutants were isolated from conidiospores of the two strains mutagenized with ultraviolet and N-methyl-N'-nitrosoguanidine. Optimal conditions for formation of intergeneric hybrids were investigated. Frequencies of intergeneric hybrid formation by nuclear transfer were
. From observations of genetic stability. DNA content. nuclear stain and conidial size. it was suggested that their karyotypes are aneuploid. In addition. the hybrids possess the 1.1~2.3-fold higher cellulase activities than those of parental strains. It was also revealed that some hybrids had different isozyme patterns compared to those of parental strains by CMCase and
-glucosidase activity assays
Physicochemical Characterization of Chlorosome Isolated from Chlorobium limicola f. thiosulfatophilum NCIB 8327
The Korean Journal of Microbiology, volume 31, issue 1, 1993, Pages 9~16
Physicochemical characteristics of chlorosomes isolated from Chlorobium lirnicoh f.thiosulfirtc~pl~ilut~i NClB 8327 were analyzed by means of UV-Visible spectrophotometer and CD-spectrophotometer. The density of the isolated chlorosomes were estimated to be 1.05 (g/
) by Percoll self gradient ultracentrifugation. Chlorosome consist of bacteriochlorophyll d and some chlorobactene, and little amounl of bacteriochlorophyll a. Chlorosome is stable from 0 to
and alkaline solution (above pH 7.0). but unstable in illuminated condition. From these results. it is suggested that some proteins or lipids may be essential for the stabilization of chlorosomes in vivo.
Characterization of Microbial Pathogen Bacillus thuringiensis Isolates from Soil Against Mosquito and Silkworm Larvae (II)
Lee, Hyung-Hoan ; Yoo, Bo-Rim ; Kim, Young-Joo ; Won, Nam-Hi ; Kim, Hak-Chun ;
The Korean Journal of Microbiology, volume 31, issue 1, 1993, Pages 17~21
Eight strains of Bacillus thuringiensis were isolated from soil in Korea and characterized. The isolates were named HL-24, HL-25, HL-33, HL-34, HL-35, HL-38, HL-39, HL-40. Strains HL-24 and HL-25 produced irregular parasporal crystals, HL-33 and HL-35 produced bipyramidal crystals, and others were round form in their cells. The biochemical characteristics of the eight isolates were only minor different in specific characteristics to the known serotypes of Bacillus thuringiensis. The HL-25, HL-33 and HL-34 strains showed resistances to cephalothin, colistin and penicillin G, and HL-39 and HL-40 strains were resistant to penicilin G. The strains of HL-24, HL-25, HL-33 and HL-34 were toxic to Bombyx mori lavae and HL-24, HL-25, HL-38, HL-39 and HL-40 strains killed Culex pipiens 3rd instar larvae. The HL-24 and H25 strains showed lethal activity against two kinds of the larvae, however lethality against mosquito larvae was low.
Cell Biological Characteristics of Trimorphomyces papilionaceus diploid
The Korean Journal of Microbiology, volume 31, issue 1, 1993, Pages 22~26
Cloning and Transcriptional Fusion with lacZ of a Gene (exo) Required for Exo-polysaccharide Synthesis in Rhizobium fredii USDA191
The Korean Journal of Microbiology, volume 31, issue 1, 1993, Pages 27~36
Stability of Recombinant Plasmids Carrying the stb Locus of E. coli IncFII NR1 Plasmid in E. coli and Yeast
Chung, Kung-Sook ; Kim, Choon-Kwang ; Kim, Kyu-Won ;
The Korean Journal of Microbiology, volume 31, issue 1, 1993, Pages 37~43
The effect of stb locus of E. COLI IncFII plasmid NR1 on the stability of chimeric plasmids was investigated. First, we have isolated the stability locus (stb) from E. coli NR1 plasmid and then inserted into the three different vectors, pUC8, YRp17 and YEp24. By examining their stability in E. coli and yeast, we showed that the recombinant plasmids containing stb locus were resonably stable. Also, by comparing the amounts of the rDNA fragments per haploid genome with those of the plasmid fragments, we showed they copy number of recombinant plasmids was not increased. Consequently, the stb locus of E. coli IncFII plasmid NR1 stabilized the chimeric plasmids but did not affect the replication or copy number of plasmids.
Cloning and Base Sequence Determination of Replication Initiation Gene (rep) Isolated from Staphylococcus aureus DH1 R-plasmid pSBK203
Park, Seung-Moon ; Kwon, Dong-Hyun ; Byeon, Woo-Hyeon ;
The Korean Journal of Microbiology, volume 31, issue 1, 1993, Pages 44~47
A replication initiation gene was identified and its nucleotide sequence has been determined from a 3.8 kb, chloramphenicol acethyltransferase conferring R-plasmid pSBK203 of Staphylococcus aures. Location of the replication related region of pSBK 203 was determined by interuption with pUC 119 at XBaI and MspI sites which resulted in inactivation of replication in Bacilius subtilis. Base sequence of this region revealed on open reading frame of 942 base pairs, which encoded a 314 amino acid protein. Base sequence homology with other rep of pT181 family plasmids such as pT181, pC221, pC223, pS194, pU112, and pCW7 was ranged from 78% to 97% and the predicted amino acid sequence homology was from 72% to 95%.
Improvement of Glucoamylase Productivity of Saccharomyces diastaticus by Intergration of Glucoamylase Gene, STA, into Chromosomal DHA
The Korean Journal of Microbiology, volume 31, issue 1, 1993, Pages 48~53
For the purpose to improve the glucoamylase productivity of Saccharomyces diastaticus, we integrated STA 1 gene into chromosomal DNA of S. diastaticus using YIp vector. After construction of Ylp-STA by the subcloning of STAI (5.3 kb) into YIp5 vector, S. diastaticus GMT-II(a. ura3. STAJ) was transformed by Ylp-STA through homologous recombination at the chromosomal STAJ gene. So we obtained the tram formants that glucoamylase productivity was increased maximum six fold. These strains transformed by the multi-copy integration of Ylp-STA in chromosomal DNA were confirmed by Southern hybridization. And the integrated Ylp-STA was maintained stably during 30 mitotic divisions.
Purification and Characterization of Adenosine deaminase from Aspergillus oryzae
Choi, Hye-Seon ;
The Korean Journal of Microbiology, volume 31, issue 1, 1993, Pages 54~62
Intracellular adenosine deaminase (ADA) from Aspergillus oryzae was purified using ammonium sulfate fractionation, a DEAE-Sephadex A-50 anion exchange chromatography, an ultrafiltration using a PM 10 membrane and two times of Sephadex G-100 gel filtration chromatography. The enzyme was purified 151 fold with a 9% recovery. Purified enzyme gave a single protein band with a molecular weight of 105,000 delton. The enzyme was reasonably stable. The enzyme activity was kept even after 1 hr incubation at 55.deg.C, but decreased significantly at 60.deg.C. The pH optimum was found to be from 6.5 to 7.5. Among tested compounds, the substrate activity was found with adenosine, adenine arainofuranoside, formymcin A, 2'-deoxyadenosine, 3'-deoxyadenosine, 2', 3'-isopropylidene adenosine, 2,6-diaminopurine deoxyriboside, .betha.-nicotinamide adenine dinucleotide (reduced form), 6-chloropurine riboside, 2'-adenine monophosphate (AMP), 3'-AMP and 5'-AMP. The values of Km of adenosine and 2'-deoxyadenosine were calculated to be 500 and .
m, respectively. ADA was sensitivite to
, p-chloromercuribenzoate and mersalyl acid inactivated the enzyme. The activity of enzyme was not changed when ADA was incubated with dithiothreititol, 2-mercaptoethanol, N-ethylmaleimide, iodoacetic acid and iodoacetamide.
Purification and Characterization of Xylanase I from Trichoderma koningii ATCC 26113
Kim, Hyun-Ju ; Kang, Sa-Ouk ; Hah, Yung-Chil ;
The Korean Journal of Microbiology, volume 31, issue 1, 1993, Pages 63~71
A xylanase (xylanase I) was purified 11.9-fold from the culture filtrate of Trichoderma koningii ATCC 26113 by the column chromatography on Sephadex G-75, SP-Sephadex C-50, DEAE-Sephadex A-50 and Sephadex G-50 with an overall yield of 8.2%. The molecular mass determined by gel filtration and sodium dodecyl sulfate polyacrylamide gel electrophoresis was found to be a monomeric polypeptide of ca. 35 kDa. The isoelectric point of the enzyme was estimated to be 9.3. The optimal reaction pH and temperature are 5.8 and 55.deg.C, respectively. The enzyme is stable up to 60.deg.C, while 78% of its activity is lost after the incubation for 10 min at 70.deg.C. The enzyme hydrolyzes sylan with relatively high activity, as well as carboxymethyl cellulose and avicel. The
values of the enzyme for oat-spelf sylan, larchwood xylan and Avicel were 3.5, 1.6 and 10. 1 mg/ml, respectively. The enzyme hydrolyzed oat-spelt sylan to sylose, sylobiose, sylotriose and arabionoxylobiose, while it degraded larchwood xylan to xylose, xylobiose, xylotriose and arabionoxylobiose, while it degraded larchwood xylan to xylose, xylobiose and xylotriose as the major products. The hydrolysis patterns indicate that xylanase I is endo-enzyme.e.
Effect of cAMP on the Replication of Human Cytomegalovirus
The Korean Journal of Microbiology, volume 31, issue 1, 1993, Pages 72~78
Since the 'promoter/enhancer region of the major immediate early (IE) ~ene of human cytomegalovirus (HCMV) contains the cyclic AMP (cAMP) response element (CRE) consensus sequence, it was reasonable to hypothesize that cAMP might affect HCMV replication. Cyclic AMP modulating drugs such as 8-bromoadenosine 3',5'-cyclic monophosphate (BrA), and papaverine were used to affect the intracellular levels of cAMP, and the effects of the drugs on HCMV replication were studied. While papaverine effectively inhibited HCMV multiplication and DNA synthesis, BrA exerted little effect on the production of infectious HCMV yields. The synthesis of DNA in HCMV-infected cells appeared to be stimulated by BrA In order to understand the effect of cAMP on the expression of HCMV major IE gene, plasmid (pCMVIE/CAT) containing a reporter gene driven by HCMV IE promoter was transfected into either permissive human embryo lung (HEL) cells or nonpermissive cells. PL,Javerine, which has been reported to block the HCMV-induced increase in cAMP, reduced the expression of pCMVIE/CA T in permissive HEL cells. Treatment of transfected cells with BrA increased the expression of HCMV major IE promoter not only in HEL cells, but also in nonpermissive HeLa and Vero cells. Therefore, it seems that the expression of HCMV major IE gene is regulated by cAMP.
Calcium Response of CHSE Cells Following Infection with Infectious Pancreatic Necrosis Virus (IPNV)
Kang, Kyung-Hee ; Park, Kee-Soon ; Lee, Chan-Hee ; Lee, Chan-Hee ;
The Korean Journal of Microbiology, volume 31, issue 1, 1993, Pages 79~84
Infection of Chinook Salmon Embryo (CHSE) cells with IPNV resulted in a significant decrease in intracellular free calcium concentration ([
]i) compared to mock-infected cells. The degree of the decrease in [Ca
]i was dependent on the amount of input virus, and treatment of IPNV-infected CHSE cells with metabolic inhibitors such as cyloheximide cordycepin partially reversed the decrease in [
]i in IPNV-infected cells. Inactiation of PINV with UV also abolished IPNV-induced decrease in [
]i. These data suggest an active role of IPNV in the decrease of [Ca
]i in the infected CHSE cells. The importance of the decrease in [
i] could be supported by the finding that the production of IPNV plaques increased in the cells treated with verapamil, a calcium influex blocker, and by lowering the concentration of extracellular calcium. Decreased production of IPNV plaques was observed by elevating the extracellular calcium. Thus, it is suggested that IPNV induced a decreased in [
]i and the decrease in [
]i may plan an importat role in efficient replication of IPNV.ation of IPNV.
Microbiological and Chemical Analyses of Paldang Lake Water
The Korean Journal of Microbiology, volume 31, issue 1, 1993, Pages 85~92
To investigate the eutrophication process and pollution characteristics in Paldang Lake, Korea, water and sediment samples were analysed during July 1986~June 1987. The transparency, chlorophyll-a concentration, dissolved oxygen concentration and biochemical oxygen demand in Paldang Lake ranged 0.5~3 m, 3-17
, 7.2~12.3 ppm and 0.5~2.3 ppm, respectively. Heterotrophic bacterial number fluctuated seasonally between
in the water column and between
in the I g dry sediment. Water turbulence and water quality of up-stream seem to play important roles for determining the water quality in Paldang Lake particularly where the hydraulic retention time is so short as about 5 days. The present water quality in Paldang Lake according to the criteria of lake water quality was shown to be between mesotrophic and eutrophic state by secchi depth(O.5 ~ 3 m) and chlorophyll-a concentration (3~17
). The distribution of coliform bacteria showed that the pollution was mainly due to the human activities in this area and it is needed to establish countmeasurements for the problems.
Seasonal Fluctuations of Heterotrophic Activity and Bacterial Extracellular Enzyme Activity in Paldang Lake
The Korean Journal of Microbiology, volume 31, issue 1, 1993, Pages 93~98
To investigate the organic matter transformation in aquatic environment, seasonal fluctuations of heterotrophic activity and microbia] extracellular enzyme activity were studied in Paldang Lake, Korea. The turnover time in the water column and the sediment at the station I fluctuated between 3 -I ,300 hrs and 17-170 hrs for glucose, 5 -1.900 hrs and 15-240 hrs for protein hydrolysate and 4-350 hrs and 15-230 hrs for acetic acid, respectively, indicating that the seasonal turnover time of organic substrates fluctuated drastically. The respiration ratios of glucose. protein hydrolysate and acetate were 23-32%, 38-41% and 22-28% in the water column and 34%, 61% and 41% in the sediment. respectively. These results showed that the respiration ratios in the sediment were higher than those in the water column regardless of kinds of organic substrates. The bacterial extracellular enzyme activities of
-D-glucosaminidase and aminopeptidase were 32-44%. 31-32%, 18-34% and 61-67% in the water column, and 34%. 40%, 23% and 65% in the sediment. respectively.