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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
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Volume & Issues
Volume 32, Issue 4 - Jan 1994
Volume 32, Issue 3 - Jan 1994
Volume 32, Issue 2 - Jan 1994
Volume 32, Issue 1 - Jan 1994
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Molecular Level Relationships of Purple Nonsulfur Bacteria and their Relatives
Lee, Sang-Seob ; Yoon, Byoung-Su ; Kim, Jae-Soo ; Lee, Hyun-Soon ;
The Korean Journal of Microbiology, volume 32, issue 1, 1994, Pages 1~6
DNA-DNA hybridization by kinetic method was carried out between species of purple nonsulfur photosynthetic bacteria and nonphotosynthetic bacteria. The degrees of homology percent were shown to be low (2-35 D%) with the exception of high homology % (72-88 D%) for strains within a species and between Rhodobacter capsulatus and Rhodopseudomonas blastica. The D% between the purple nonsulfur photosynthetic bacteria, Rhodopseudomonas palustris, and nonphotosynthetic bacteria, Pseudomonas aeruginosa ATCC 27853 or Bradyrhizobium japonicum were a little higher (26-33 D%) than the D% between any other photosynthetic bacteria. The homology % between Rhodopseudomonas blastica and Rhodobacter capsulatus was 72 D%, which showed genetic relationship.
The Aerobic Nature of Arcobacter nitrofigilis
Han, Yeong-Hwan ;
The Korean Journal of Microbiology, volume 32, issue 1, 1994, Pages 7~11
The free-livein nitrogen fixing bacterium, Arcobacter nitrofigilis which has been known to be a microaerophile, exhibited aerobic growth in brucella broth. In a level of oxygen equivalent to an air atmosphere (21%
), the maximum cell growth was observed in brucella broth. Low level of cell growth occurred in a level of low oxygen equivalent to lower than 2%, unless any other terminal electron acceptors other than oxygen were wupplied in brucella broth. Membrane-bound cytochrome b and c, and soluble cytochrome c were found. The growth in an aerobic atmosphere, little growth at low oxygen level, and occurrence of cytochrome c mean that this species is an aerobe and obtains energy using energy-yielding respiration.
Localization of a KEM1::lacZ Fusion Protein in Yeast Cells
Kim, Jin-Mi ; Fink, Gerald R. ;
The Korean Journal of Microbiology, volume 32, issue 1, 1994, Pages 12~19
KEM1 is known to control the spindle pole body or microtubule function, probably in response to the cellular nutritional conditions in Saccharomyces cerevisiae. Transposon insertions were performed in the cloned KEM1 gene using mini-Tn10-LUK element carrying E. coli
-galactosidase structural gene. A collection of ranfom Tn10-LUK insertions defined an approximately 3.5 kb region required for the KEM1 function. From this collection functional KEM1::lacZ protein fusions were identified. Indirect immunofluorescence using anti-
-galacatosidase antibodies localized the KEM1::lacZ fusion protein to the periphery of the nucleus.
Genetic Organization of an Inducible
-Lactamase Gene Isolated from Chromosomal DNA of Staphylococcus aureus
Kim, Young-Sun ; Min, Kyung-Il ; Byeon, Woo-Hyeon ;
The Korean Journal of Microbiology, volume 32, issue 1, 1994, Pages 20~27
-lactamase gene (bla) was identified and isolated from the chromosomal DNA of multiple drug resistant strains of Staphylococcus aureus. Determined base sequence of bla and of its flanking region was compared with those of bla genes identified on the staphylococcal plasmids pPC1, pI258, pI1071, and pUB101. Base sequence of 843 base-long structural gene of our bla was same as that of pPCl-, pI258-, and pS1-bla. However, HindIII recognition site Which is found in most of the bla genes at 140 base upstream from the structural gene was moved to the site of 370 base upstream from the structural gene. And one of the two direct repeat sequence found in downstream flanking region of pI1071-bla was deleted in our bla. Amino acid sequence homology analysis of the ORF located around HindIII recognition site reveals that this 80 amino acids-long polypeptide is C-terminus of transposase of Tn4001.
Cloning and Experssion of a Human tau Gene cDNA in Escherichia coli
Chung, Sang-Ho ; Maeda, Tadakazu ; Yanagawa, Hiroshi ;
The Korean Journal of Microbiology, volume 32, issue 1, 1994, Pages 28~33
In normal cells tau protein is associated with axonal microtubules, whereas in Alxheimer's disease it is immobilized in the somatodendritic compartment of certain nerve cells as a major component of the paired helical filament. As a part of the study to analyze the nature of the paired helical filament (PHF) deposits and some related factors in brain, we have cloned and expressed a human tau gene cDNA in Escherichia coli to obtain the recombinant human tau protein in abundance.
Molecular Cloning of
-Amylase Gene from Schwanniomyces CBS 2863
Park, Jong-Chun ; Bai, Suk ; Chun, Bai-CHun ;
The Korean Journal of Microbiology, volume 32, issue 1, 1994, Pages 34~39
The gene encoding
-amylase of Schwanniomyces castellii was cloned in Saccharomyces cerevisiae. The 5.0-kilobase insert was shown to direct the synthesis of
-amylase. Southern blot analysis confirmed that this
-amylase gene was derived from the genomic DNA of Sch. castellii. Immunoblot analysis showed that
-amylase production from S. cerevisiae transformant was less than that of donor strain. The
-amylase secreted from S. cerevisiae transformant was shown to be indistinguishable from that of Sch. castellii on the basis of molecular weight and enzyme properties.
Cloning and Expression of pcbCD Genes in Escherichia coli from Pseudomonas sp. DJ-12
Kim, Chi-Kyung ; Sung, Tae-Kyung ; Nam, Jung-Hyun ; Kim, Chang-Young ; Lee, Jae-Koo ;
The Korean Journal of Microbiology, volume 32, issue 1, 1994, Pages 40~46
The pcb genes of Pseudomonas sp. DJ-12 coded for the catabolism of polychlorinated biphenyl (PCBs) and biphenyl. The products of the pcbCD genes were 2,3-dihydroxy-4'-chlorobiphenyl dioxygenase and meta-cleavage product (MCP) hydrolase, which acted on degradation of 2,3-dihydroxy-4'-chlorobiphenyl to 4-chlorobenzoate. The pcbCD genes were cloned in E. coli XLl-Blue, and then the pcbD gene was further subcloned. As a metabolite transformed from 2,3-dihydroxybiphenyl by the cloned cell of E coli CU103, benzoate was detected by the resting cell assay. The enzyme activities of 2,3-dihydroxybiphenyl dioxygease and MCP hydrolase produced in the cloned cells E. coli CU103 and CU105 were about 17 and 3 times higher than those of Pseudomonas sp. DJ-12, respectively.
Chaperon Effects of Campylobacter jejuni groEL Genes Products in Escherichia coli
Lim, Chae-Il ; Kim, Chi-Kyung ; Lee, Jae-Kil ;
The Korean Journal of Microbiology, volume 32, issue 1, 1994, Pages 47~52
The cells of Campylobacter jejuni heat-shocked at 48
for 30 min synthesized the heat shock proteins of HSP90, HSP66 and HSP60. Those heat shock proteins were found to correspond to the heat shock proteins of HSP87, HSP66 (DnaK), and HSP58 (GroEL) of E. coli, respectively. By Southern blot analysis of the chromosomal DNAs of C. jejuni with groESL and dnaK genes of E. coli as DNA probes, the heat shock genes of C. jejuni which are homologous to the E. coli groESL and dnaK genes were found to exist in the chromosomal DNA. The genomic libraries of C. jejuni were constructed with the cosmid vector pWE15 and the groEL gene of C. jejuni were cloned in E. coli B178 groEL44 temperature senstive mutant. The hybrid plasmid (pLC1) was inserted with the DNA fragment (about 5.7kb in size) containing the groEL gene. E. coli groEL44 mutant cell transformed with the pLC1 could grow at 42
by synthesizing the HSP60 of C. jejuni and regained the susceptibility to the
vir phage by expression of the groEL gene in the cloned cells. These indicated that the groEL products of C. jejuni had chaperon effects by synthesizing the heat shock proteins in the cloned cells of E. coli.
Host Construction by Curing the Octopine Type Ti and Cryptic Plasmids in Agrobacterium tumefaciens KU12
Ha, Un-Hwan ; Lee, Yong-Woog ; Moon, Hye-Yeon ; Sim, Woong-Seop ;
The Korean Journal of Microbiology, volume 32, issue 1, 1994, Pages 53~59
Agrobacterium tumefaciens KU12 contains pTiKU12 (240kb) of the octopine type Ti plamsid and pTi12 (45 kb) of the cryptic plasmid. To make the avirulent A. tumefaciens, the octopine type Ti plasmid, pTiKU12, was cured with elevated temperature (37
) and ethidium bromide (EtBr), respectively. Also the cryptic plasmid, pTi12, was cured by the introduction of recombinant plasmid, pYWXP, made by pTi12 replication origin and pUC19. pYWXP was cured by elevated temperature (37
) and EtBr simultaneously.
Studies on the Isolation and Characterization of the Pseudomonas syringae pv. tabaci Phage
Jun, Hong-Ki ; Kim, Tae-In ; You, Jin-Sam ; Baik, Hyung-Suk ;
The Korean Journal of Microbiology, volume 32, issue 1, 1994, Pages 60~64
Pseudomonas syringae pv. tabaci produces tabtoxin and causes wildfire disease on tabacco and bean plants. In this study, bacteriophage of P. syringae pv. tabaci were isolated from sewage by top agar overlay method, and physiological and genetical characteristics of the phage were investigated. Plaques of isolated phage were turbid and ranged in size from 1 to 2 mm. The stability range of pH was between 6.0 and 9.0, and stability of temperature was up to 30
and inactivated at 70
. The adsorption rate of phage was about 85% for 30min. The latent period and mean burst size as dertermined in one step growth experiments were 3 hrs and 200 PFU/bacterium, respectively. Genomic material of isolated phage was dsDNA of which size was about 30kb.
Influence of the Photosynthesis of Synechococcus sp. on the Development of its Cyanophage
Kim, Min ; Choi, Yong-Keel ;
The Korean Journal of Microbiology, volume 32, issue 1, 1994, Pages 65~69
Light appears to be needed in the early and late function of the cyanophage of Synechococcus sp. and dark treatment during the first 2 hr of the replication cycle increased the virus yield to 200%. The burst size of the cyanophage multiplied in Synechococcus sp. in dark was 11% of that of control. The viral multiplication was reduced 2% in the presence of photosynthetic inhibitor, DCMU of
M, and nearly blocked in
M CCCP. These data suggested that the photosynthetic dependence of the cyanophage is greater than those of LPP-1 and AS-1, and smaller than SM-1.
Antibiotic Biosynthesis in bldA-like Mutant of Strptomyces coelicolor
Park, Unn-Mee ;
The Korean Journal of Microbiology, volume 32, issue 1, 1994, Pages 70~77
The author isolated 7 mutant candidates which mapped around cysA (which was 10 o'clock). They were divided into two groups. One of them was located counterclockwise to cysA, and the other was clockwise to cysA. Since bldA was mapped counterclockwise to cysA, the candidate which mapped counterclockwise to cysA was transduced with phage containing wild type bldA gene clone. The candidates might be the alleles of bldA, because they were complemented by bldA clone. However some of such mutants sporulated very well and developed as much pigment as wild type on rich media plate. Their phenotype was not like bld mutant at all on such conditions. There were real antibiotics gene expressions, since transcriptional reporter gene xylE had shown high activities. Majority of the bldA like mutants showed act gene expressions when they were transformed with high copy number plasmid containing actII-ORF4.
Purification and Properties of a Membrane-bound Alcohol Dehydrogenase from Acetobacter sp. HA
Yoo, Jin-Cheol ; Sim, Jung-Bo ; Kim, Heung-Keun ; Chun, Hong-Sung ; Kim, Sung-Jin ;
The Korean Journal of Microbiology, volume 32, issue 1, 1994, Pages 78~83
Membrane-bound alcohol dehydrogenase(ADH) was purified to homogeneous state fron an acetic acid producing bacteria, Acetobacter sp. HA. The enzyme was purified about 153-fold with an overall yield of 35% from the crude cell extract by solubilization and extraction of the enzyme with Triton X-100 and subsequent fractions by column chromatography. Upon sodium dodecyl sulphate-PAGE, the enzyme showed the presence of three subunits with a molecular mass of 79,000 daltons, 49,000, and 45,000 daltons, respectively. Absorption oxidized aliphatic alcohols with a straight carbon chain except for methanol. Formaldehyde, acetaldehyde and glutaraldehyde were also oxidizable substrates. The apparent
for ethanol was 1.38mM. The optimun pH and temperature were 5.0~6.0 and 32
and heavy metals such as
were inhibitory to the enzyme activity.
Kinetics of Intracellular Adenosine Deaminase to Substrate Analogs and Inhibitors in Aspergillus oryzae
Choi, Hye-Seon ;
The Korean Journal of Microbiology, volume 32, issue 1, 1994, Pages 84~90
Kinetic parameters of various substrates and inhibitors were measured to elucidate the binding requirements of the active site of intracellular adenosine deaminase (ADA) in Aspergillus oryzae. 3'-Deoxyadenosine was the best substrate according to the value of relative kcat/
. Purine riboside was found to be the strongest inhibitor with the
M. Adenine acted neither as a substrate nor as an inhibitor, suggesting the presence of ribose at N-9 of adenosine was crucial to binding. ADA also catalyzed the dechlorination of 6-chloropurine riboside, generating inosine and chloride ions. Substrate specificity of 6-chloropurine riboside was 0.86% of adenosine. Purine riboside, a competitive inhibitor of ADA, inhibit the dechlorination with similar
value, suggesting that the same binding site was involved in deamination and dechlorination. Among the sulfhydryl group reagents, mercurials, pchloromercuribenzoate (PCMB), mersalyl acid and
inactivated the enzyme. Mersalyl acid-inactivated ADA was reactivated by thiol reagents, but PCMB-inactivated enzyme was not. When ADA was treated with the mercurial reagents, the inhibition constants and inhibition patterns were determined. Each inhibition was competitive with substrate. The
values of these mercurial reagents were lower in 10 mM phosphate buffer than in 100 mM phosphate buffer, showing phosphate dependency.
Characterizations of Restriction Endonuclease EagBI from Enterobacter agglomerans CBNU45
Choe, Yeong-Ju ; Kim, Seong-Jae ; Hwang, Hye-Yeon ; Im, Jeong-Bin ; Kim, Yeong-Chang ;
The Korean Journal of Microbiology, volume 32, issue 1, 1994, Pages 91~95
EagBI is a type II restriction endonuclease from Enterobacter agglomerans strain CBNU45 isolated from soil. EagBI was partially purified by DEAE-cellulose, phosphocellulose P11 and hydroxylapatite column chromatography. EagBI recognizes and cleaves the sequence 5'-CGAT
CG-3' and generates 2-base 3'-protruding cohesive ends. The optimal reaction conditions of EagBI are 10 mM Tris-HCl (pH 7.8), 6-10 mM
, at 37
. The enzyme is maximally active in the absence of NaCl, able to cleave both
DNAs, and sensitive to heat treatment (at 65
for 10 min). Therefore, although EagBI is an isoschizomer of PvuI, it is more useful than PvuI in respect of the NaCl requirement and heat-stability.