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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Microbiology
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Journal DOI :
The Microbiological Society of Korea
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Volume & Issues
Volume 32, Issue 4 - Jan 1994
Volume 32, Issue 3 - Jan 1994
Volume 32, Issue 2 - Jan 1994
Volume 32, Issue 1 - Jan 1994
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Isolation and Microbiological Characterization of Azospirillum from the Rhizosphere of Oryza sativa L. in Korea
Kim, Won-Gon ; Seo, Hyun-Chang ; Kim, Jong-Pyung ; Kim, Chang-Jin ; Lee, Ke-Ho ; Yoo, Ick-Dong ;
The Korean Journal of Microbiology, volume 32, issue 2, 1994, Pages 97~101
Fifteen strains of the nitrogen fixer Axospirillum were isolated from the rhizosphere of rice collected from Kyonggi-do and Chungcheongnam in Korea. They had strong acetylene-reducing activity of 400 of 900 nmol
per hour vial had a similar morphology in succinate-malate medium: vibrioid cells having a diameter of 1.0
and a monopolar single flagellum in liquid media. According to their physiological and morphological characteristics, they were divided into two distinct groups, group I and group II. Group I strain were, unlike group II, distinguished by their ability to use glucose as a sole carbon source in nitrogen-free medium, requirement for biotin, and formation of wider, longer, and S-shaped cells in semisolid nitrogen-free malate medium. On the basis of their characteristics, strains belonging to group I were identified as Azospirillum lipoferum, while those belonging to group II were identified as Azospirillum brasilense.
Structure-Function Analysis of DNA Binding Domain of the Yeast ABF1 Protein
Cho, Gi-Nam ; Lee, Sang-Kyung ; Kim, Hong-Tae ; Kim, Ji-Young ; Rho, Hyune-Mo ; Jung, Gu-Hung ;
The Korean Journal of Microbiology, volume 32, issue 2, 1994, Pages 102~108
Autonomously replicating sequence Binding Factor 1(ABF1) is a DNA-binding protein that specifically recognizes the
at many sites in the yeast genome including the promoter element, mating-type silencer and ARS. To express the intact full-length ABF1 gene in E. coli, the ABF1 gene has been cloned into pMAL-c2 and His-61, Leu-353 and Leu-360 were substituted with other amino acid. ABF1 fusion proteins of wild type ABF1 and H61A, L353R and L360R nutants were purified by amylose resin affinity chromatography. Fusion protein of MBP and ABF1 was digested by Factor Xa and Characterized by gel retardation assay and complementation test. As aresult, we suggested that other DNA binding motif except atypical inc-finger motif is in the middle region of ABF1.
Repression of Escherichia coli serC-aroA Operon by Aromatic Amino Acids
Hwang, Woo-Gil ; Sa, Jae-Hoon ; Kim, Kyung-Hoon ; Lim, Chang-Jin ;
The Korean Journal of Microbiology, volume 32, issue 2, 1994, Pages 109~114
The Escherichia coli aroA and serC genes constitute a mixed-function operon which involves in two different amino acid biosynthetic pathways. The regulation of expression of serC-aroA operon was evaluated through the use of a serC-araA-lacZ fusion plasmid pWH2. The expression of the serC-aroA operon was decreased by aromatic amino acids such as tyrosine, tryptophan, and phenylalanine. The repressible effects were diminished in E. coli tyrR of trpR strain, indicating the involvemnt of TyrR of TrpR protein in the repression. Tyrosine was competitie with cAMP in the influence on the expression of the serC-AroA operon. From these data, it was suggested that the serC-aroA operon is controlled by aromatic amino acids in a negative manner.
Cloning, Base Sequence Determination and Homology Analysis of Replication Controlling cop Gene of R-plasmid pSBK203 Isolated from Staphylococcus aureus DHI
Park, Seung-Moon ; Byeon, Woo-Hyeon ;
The Korean Journal of Microbiology, volume 32, issue 2, 1994, Pages 115~119
Replication control region of pSBK203, a chloramphenicol acetyltransferase conferring plasmid from Staphylococus aureus was cloned and its nucleotide sequence has been determined. Base sequence homology of this copy control region with those of plasmids belonging to pT181 family was obtained and analyzed. Copy number of four copy mutants derived by addtion or deletion of nucleotides in unique XbaI recognition site in copy control region of pSBK203 was also determined.
Cloning and Sequence Analysis of the trpB, trpA and 3' trpC(F) Gens of Vibrio metschnikovii Strain RH530
Kwon, Yong-Tae ; Kim, Jin-Oh ; Yoo, Young-Dong ; Rho, Hyune-Mo ;
The Korean Journal of Microbiology, volume 32, issue 2, 1994, Pages 120~125
The genes, trpB, trpA and 3’ trpC(F) of Vibrio metschnikovii strain RH530 were cloned and sequenced. The trpB and trpA genes had open reading frames of 1,173 bp and 804 bp encoding 391 and 268 amino acids, respectively. The trpB and trpA genes had conventional ribosome-binding sequences and overlapped with each other by one nucleotide, suggesting that these two genes are translationally coupled. 115 nucleotide upstream the trpB start codon, tjere was an incomplete open reading frame of the 3’-end of the trpC(F). The amino acid sequences of trpB, trpA and trpC(F) of V. metschnikovii RH530 had identities of 64.2%, 82.4% and 73.7% respectively, for those of V. parahaemolyticus; 58.7%, 72.3% and 54.9%, respectively, for Salmonella typhimurium; and 42.6%. 54.1% and 12.5%, respectively, for brevibacterium lactofermentum. The genetic organization of these genes, especially in the noncoding region between trpC(F) and trpB, was distinct from that of Enterobacteriaceae.
Cloning of Dechlorination Genes Specifying Biodegradation of Toxic 4-Chlorobiphenyl
Kim, Chi-Kyung ; Chae, Jong-Chan ; Han, Jae-Jin ;
The Korean Journal of Microbiology, volume 32, issue 2, 1994, Pages 126~131
The pchABCD genes in Pseudomonas sp. DJ-12 speciyin degradation o 4-chlorobiphenyl(4CB) were cloned in Eschericia coli. The cloned cells of E. coli CU1 and CU101 showed to produce 2,3-dihydroxybiphenyl (2,3-DHBP) from 4-chlorobiphenyl by dechlorination, as Pseudomonas so. DJ-12 produced 2,3-DHBP from both biphenyl and 4CB. In particular, E. coli CU101 transformed with the recombinant plasmid of pCU101 revealed dechlorination activity to produce 2,3-DHBP from 4CB without production of 4-chlorobenzoic acid. Therefore, the pcbAB genes (2.2 kb in size) cloned from the chromosome of Pseudomonas sp. DJ-12 were found to have dechlorination activity on 4CB to produce 2,3-DHNP.
Isolation and Characterization of L-Ascorbic Acid-Producing Enzyme in Neurospora crassa
Kim, In-Sil ; Lee, Yeon-Hee ;
The Korean Journal of Microbiology, volume 32, issue 2, 1994, Pages 132~138
L-Ascorbic acid-producing enzyme in Neurospora crassa was found to exist in mitochondria and the activity of this enzyme was increased by the addition of D-fluconno-
-lactone or L-gulono-
-lactone in the media. L-Ascorbic acid-producin enzyme in N. crassa has been purified with ammonium sulfate precipitation. DEAE Sepharose CL-6B ion exchange chromatography. Sephacryl S-200 gel filtration chromatography and Reactive yellow 3-agarose dye affinity column chromatography. The specific activity of this enzyme was increased to 239.6 fold and the yield was 2.1%. The molecular weight of the native enzyme was 150.000 dalton when it was estimated with Sephacryl S-200 gel filtration chromatography. Its molecular weight appeared as 75.000 dalton by SDS-polyacrylamide gel electrophoresis. which suggested that this enzyme was consisted with two identical subunits. The optimal pH for this enzyme was 9.0 and the
value for D-galactono-
-lactone was 0.073 mM.
Functional Characteristics of Cytoplasmic and Periplasmic Photobacterium leiognathi CuZnSOD (PSOD) in Escherichia coli SOD Double Mutants
Kim, Young-Gon ; Yang, Mi-Kyung ;
The Korean Journal of Microbiology, volume 32, issue 2, 1994, Pages 139~146
Protective effects on subcellular localization of Photobacterium leiognathi CuZnSOD(PSOD) were examined in Escherichia coli SOD mutant cells on the treatment of paraquat, heat shock
, hydrogen peroxide and copper sulfatem respectively. The physiological characteristics of the periplasmic and cytoplasmic PSOD localized differently are dependent on the conditions in this experiment. Cells expressing SOD periplasmically in the treatments of paraquat and
respectively were somewhat better protective effects cells expressiong SOD cytoplasmically at comparable level and SOD expression level showed, the most consistently important variable. However, this was reversed in the treatments of heat shock and
Purification and Cellular Localization of Extracellular Nuclease of Serratia marcescens Expressed in Escherichia coli
Kim, Woe-Yeon ; Lee, Hoon-Sil ; Suh, Sook-Jae ; Cho, Moo-Je ; Lee, Sang-Yeol ; Kim, Jae-Won ;
The Korean Journal of Microbiology, volume 32, issue 2, 1994, Pages 147~154
Nuclease was secreted to the environmental media from the Escherichia coli JM107 tranformant harboring the extracellular nuclease gene of Serratia marcescens in the plasmid of pNUC4. Under the growth conditions, the amount of secreted enzyme was increased in parallel with bacterial growth conditions, the amount of secreted enzyme was increased in parallel with bacterial growth. The enzyme was purified using chromatofraphic procedures of Matrex green gel and heparin agarose affinity gel, resulted in 50-fold purification with 15% recovery of the enzyme. The apparent molecular weight of the enzyme was estimated to be 29Kda by sodium dodecylsulfate denaturing gel electrophoresis. Using the purified enzyme, polyclonal antibody was obtained from the rabbit. The specificity of the antibody was confirmed by immunoblotting and immunoprecipitaion. For the investigation of cellular distribution of the enzyme, cells were fractionated into three fractions; cytoplasm, periplasm and extracellular fluid. While more than 80% of the enzymatic activity was detected in the extracellular fluid and periplasm, a little was found in the cytoplasm, indicating that the enzyme was likely to be immediately exported to the membrane for excretion after biosynthesis. These results were confirmed again by immunocytochemistry technique using the antibody.
Catalases in Acinetobacter sp. Strain JC1 DSM 3803 Growing on Glucose
Shin, Kyoung-Ju ; Ro, Young-Tae ; Kim, Young-Min ;
The Korean Journal of Microbiology, volume 32, issue 2, 1994, Pages 155~162
Cells of Acinetobacter sp. strain JC1 DSM 3803, an aerobic monoxide-oxidizing bacterium, growing on glucose exhibited high catalase activity at the mid-exponential growth phase. The enzyme activity decreased gradually after then until the early stationary phase, increased again at the mid-stationary phase, and then decreased again thereafter. Cells growing on glucose was found to contain three kinds of catalses. Cat1, Cat2 and Cat3. The activities of Cat1 and Cat3 did change significantly during growth, but that of Cat2 exhibited significant variation. Cat3 was found to present only in cells growing on glucose, but not in cells growing on carbon monoxide of methanol. The activities of call and Cat3 in cell-free extracts were stable upon treatment with ethanol and chloroform, but decreased to some extent when the enzymewere treated with 2mM
and/or 3-amino-1,2,4-triazole (AT). Cat2 was found to be extremely sensitive to the ethanol-chloroform and
treatments, but was insensitive to the AT treatment. Cat1 exhibited enzyme activity after incubation for 1 min at 80
. Cat2 and Cat3 did not show enzyme activity after incubation for 1 min at 60
, respectively. Cat2 was found to have peroxidase activity. Cat3 was purified to homogenity in seven steps. The molecular weight of the native enzyme was estimated to be 150,000. Sodium dodecyl sulfate-gel electrophoresis revealed two identical subunits of molecular weight 65,000. The enzyme was found to show two
values of 39 mM and 58mM. The optimal pH for the enzyme activity was 7.0, but the activities at pH 6.0, 8.0, and 9.0, were found to be comparable to that at the optimal pH. The optimal temperature for the enzyme activity was found to be 40
. The enzyme also exhibited strong activity at 20
, and 50
. The purified enzyme was not affected by the ethanol-chloroform treatment. The enzyme, howerver, showed less than 10% of the original activity when it was treated with 12 mN AT, 0.1 mM
of 1mM KCN.
Effects of Diesel Oil on the Population and Activity of Soil Microbial Community
Seo, Eun-Young ; Song, Hong-Gyu ;
The Korean Journal of Microbiology, volume 32, issue 2, 1994, Pages 163~171
The effects of diesel oil on the microbial community in sandy loam soil were investigated, and the effects of bioremediation which was performed to enhance the removal of diesel oil from soil were also measured. The residual percentage of diesel oil was about 50% after 16 week incubation period. The bioremediation treatment increased the removal rate at 60~95%. When the soil was contaminated with diesel oil, the direct bacterial count, length of fungal hyphae, aerobic heterotroph and hydrocarbon degrader were increased by 2~3 orders of magnitude. The bioremediation further increased these numbers 10 to 100-fold. There were no difinite patterns of change in fluorescein diacetate hydrolysis activity in bioremediation-untreated soil, but about 10 times of increase of activity was observed in bioremediation-treated soil. Similar change was occurred in soil dehydrogenase activity.
Biodegradation of Saturated Hydrocarbons by Xanthomonas campestris M12
Choi, Soon-Young ; Lee, Myung-Hye ; Hwang, Moon-Ok ; Min, Kyung-Hee ;
The Korean Journal of Microbiology, volume 32, issue 2, 1994, Pages 172~175
Xanthomonas campestris M12 carrying OCT plasmid which could dissimilate octane was able to utilize n-alkanes of eight to sixteen carbon atoms via the capacity of this plasmid. M12 strain could utilize terminal oxidation products of these primary, alkanes, alcohols, aldehydes and fatty acids but not hexanoic acid, adipic acid, pimelic acid and heptanal. This strain also biodegraded n-alkanes by monoterminal or diterminal oxdation of straight-chain fatty acids, and branched-chain alkane.