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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Microbiology
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Journal DOI :
The Microbiological Society of Korea
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Volume & Issues
Volume 32, Issue 4 - Jan 1994
Volume 32, Issue 3 - Jan 1994
Volume 32, Issue 2 - Jan 1994
Volume 32, Issue 1 - Jan 1994
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Phylogenetic Study of Ganoderma applanatum and Schizopora paradoxa Basd on 5S rRNA Sequences
Kim, Hak-Hyun ; Jung, Hack-Sung ;
The Korean Journal of Microbiology, volume 32, issue 3, 1994, Pages 177~181
The sequences of the cytoplasmic 5S rRNAs(EMBL accession number X73589 and X73890) from two polupores, Ganoderma applanatum and Schizopora paradoxa, were determined by the direct chemical method for sequencing RNA and compared to the sequences of 9 reported mushrooms. 5S rRNAs of Ganoderma applanatum and Schizopora paradoxa consisted of 118 bases and fit the secondary structure model of the 5S rRNAs of basidiomycetes proposed by Huysmans et al. Based on Kimura’s K_nuc values, the closest fungus to Ganoderma applanatum was Ceratobasidium cornigerum and the one to Schizopora paradoxa was Bjerkandera adusta. When the secondary structures of 5S rRNAs of 11 mushrooms were compared the base substitution occurred at helix regions more than at loop regions. When a phylogenetic tree was constructed using the Neighbor program of the PHYLIP package, it partially discriminated and separated the mushrooms of the Hymenomycetes by the order.
Decolorization of Poly R-478 Dye by Coriolus versicolor IFO 30388
Yoon, Kyung-Ha ;
The Korean Journal of Microbiology, volume 32, issue 3, 1994, Pages 182~185
Effects of nitrogen and carbon sources on the decolorization rate of poly R-478 dye by a white rot basidiomycete Coriorus versicolor IFO 30388 were examined. The fungus exhibited 87.2% of decolorization rate when it was cultured in the state of stationary in a nitrogen-limited medium (pH 4.5) which contained 2.0% glucose, 0.04% ammonium tartrate, 0.02% poly R-478 dye, 2%
, 0.002% thiamine-HCl and 10 mM 2,2 dimethylsuccinate (sodium) at
for 10 days. Decolorization of the dye occurred in the presence of nitrogen source in the medium and decolorization rate increased rapidly after depletion of
from the medium.
Replication origin (ori) of R-plasmid pSBK203 Isolated from Staphylococcus aureus DHI
Min, Kyung-Il ; Byeon, Woo-Hyeon ;
The Korean Journal of Microbiology, volume 32, issue 3, 1994, Pages 186~191
The origin of the leading strand replication (ori) and of lagging strand replication (M-O) of R-plasmid pSBK203 was identified and its base sequence was determined. About 50 bp of ori sequence residues overlapped with the structural gene of rep. Sequence comparison reveals that pSBK-ori shares obvious identities with those of pT181 family and consists of two regions, one is conserved and the other is variable region. Of two palindrome sequence located one after another in upstream region of rep gene, palA' instead of palA which shares sequence homology with diverse family of plasmids such as pOX6, pC194, and pE194 seems to act as a signal for conversion of primarily replicated ssDNA to dsDNA (minus origin (M-O)).
Evience that a Plasmid Endoces Genes for Metabolism of Malonte in Pseudomonas fluorescens
Kim, Yu-Sam ; Kim, Eun-Joo ;
The Korean Journal of Microbiology, volume 32, issue 3, 1994, Pages 192~197
Pseudomonas fluorescens which is able to utilize malonate as a sole carbon source was found to contain a novel 60 kb plasmid, which encodes the genes for the proteins to assimilate malonate, including malonate decarboxylase and acetyl-CoA synthetase. The evidence is as follows: The Pseudomonas cured with mitomycin C was unable to grow on malonate-medium as well as it lost plasmid. The plasmid isolated from the Pseudomonas could be introduced into E. coli strain JM103 and DH1 by transformation. The transformed E. coli was able to grow on malonate-medium and could transmit its plasmid back to the cured P. fluorescens by conjugation. The existence of the plasmid in the transformed E. coli was confirmed by hybridization with a labeled probe prepared from 12 kb segment of the plasmid. Dot hybridization showed that the copy number of the plasmid in the transformed E. coli is at least 13 times higher than in the wild type P. fluorescens. The two key enzymes, malonate decarboxylase and acetyl-CoA synthetase, were inducible by malonate in the transformed E. coli.
Sucleotide Sequence of the Cohesive End Site of Lactobacillus casei Phage J1 Genome
Kim, Young-Chang ; Seong, Hark-Mo ; Gang, Hyeon-Sam ;
The Korean Journal of Microbiology, volume 32, issue 3, 1994, Pages 198~201
The nucleotide sequence of the cohesive end site (cos) of Lactobacillus casei phage J1 genome was determined. Comparison between the nucleotide sequences of the circular cos and the left end of the linear J1 DNA showed that the nicking sites of the terminase were as follows: 5'- GGTCGGCC
CCAGCCGG -5' The cohesive single-stranded ends of J1 were found to be 3'-protruding and composed of 8 nucleotides. The mol% G + C of the cohesive ends was 87.5. The cos site shows dyad symmetry from -33 to + 25 bp if the 5' terminal nucleotide of the left end of the linear J1 DNA is numbered +1. No homology was found among the cos sites of phages reported so far.
Isolation and Characterization of a Mutant Defective in Light-activated Heterotrophic Groth from Synechocystis sp. PCC 6803
Park, Mi-Seon ; Lee, Young-Sook ; Kim, Young-Chang ;
The Korean Journal of Microbiology, volume 32, issue 3, 1994, Pages 202~207
A mutant strain PRM1 defective in light-activated heterotrophic growth was isolated from Synechocystis sp. PCC 6803. PRM1 could be grown at growth rate equivalent to Synechocystis 6803 under mixotrophic growth conditions. However, PRM1 could not be grown under light-activated heterotrophic conditions, in which a daily pulse of light for 5 min was given. These results suggest that PRM1 is not defective in heterotrophic metabolism, but in the transduction pathway of light signal essential to the growth. Plasmid patterns, absorption spectra of whole cells, and the exterior and interior structures of PRM1 were similar to those of Synechocystis 6803, except that PRM1 could not produce amorphous slime holding cells together.
Characterization of a New Type II Restriction Endonuclease Isolated from streptoverticillium olivoverticillatum
Hwang, Hye-Yeon ; Yim, Jeong-Bin ;
The Korean Journal of Microbiology, volume 32, issue 3, 1994, Pages 208~214
We screened many species from a wide variety of bacterial genera for a new type II restriction endonuclease. The purification and characterization of SolI from a soil isolate, Streptoverticillium olivoverticillatum are described here. The enzyme turned out to be an isoschizomer of BamHI. It recognized the hexanucleotide sequence of 5'-G
GATCC-3' and cleaved as in dicated by the arrow, generating a 4 base 5' extension. Unlike its isoschizomer, BamHI, the activity was sensitive to dam methylation within the recognition sequence. Following ammonium sulfate fractionation of the crude extract, heparin-agarose and Affi-gel Blue column chromatography were employed to purify the enzyme. SolI required at least 0.2 mM of
for the cleavage to occur. The enzyme exhibited its maximal activity in the absence of NaCl, but was inhibited completely in the presence of 120 mM NaCl. The pH and temperature optima for activity were pH 8.6 and
, respectively. The molecular weight of SolI was estimated to be 43,000 Da by Superose-12 gel filtraion chromatography.
Nucleotide Sequence of the Penicillin G Acylase Gene from Bacillus megaterium and Characteristics of the Enzyme
Gang, Ju-Hyeon ; Kim, Seong-Jae ; Park, Yong-Chjun ; Hwang, Young ; Yoo, Ook-Joon ; Kim, Young-Chang ;
The Korean Journal of Microbiology, volume 32, issue 3, 1994, Pages 215~221
The complete nucleotide sequence of the cloned pga gene encoding the penicillin G acylase of Bacillus megaterium ATCC 14945 and its 5'- and 3'-flanking regions was determined. The sequence revealed only one large open reading frame (2,406 hp) of the penicillin G acylase (pga) gene. Upstream from ATG of the pga gene, there was a putative ribosome binding site, Shine-Dalgarno sequence. The promoter-like structure, - 10 and - 35 sequences, was also found. Following the stop codon, TAG, a structure reminiscent of the E. coli rho-independent transcription terminator was present. The amino acid sequence was deduced from the nucleotide sequence. The molecular mass of the polypeptide was 91,983 Da. There was a potential signal sequence in its amino-terminal region. A comparison of its deduced amino acid sequence with other characterized penicillin G acylases and the result of SDS-polyacrylamide gel electrophoresis of the purified enzyme showed that a precursor polypeptide of 92 kDa was processed into two dissimilar
-subunits of 25 and 61 kDa.
Reaction Mechanism of Purine Nucleoside Phosphorylase and Effects of Reactive Agents for SH Group on the Enzyme in Saccharomyces cerevisiae
Choi, Hye-Seon ;
The Korean Journal of Microbiology, volume 32, issue 3, 1994, Pages 222~231
Kinetic analysis was done to elucidate the reaction mechanism of purine nucleoside phosphorylase (PNP) in Saccharomyces cerevisiae. The binary complexes of PNP
phosphate and PNP
ribose 1-phosphate were involved in the reaction mechanism. The initial velocity and product inhibition studies demonstrated were consistent with the predominant mechanism of the reaction being an ordered bi, bi reaction. The phosphate bound to the enzyme first, followed by nucleoside and base were the first product to leave, followed by ribose 1-phosphate. The kinetically suggested mechanism of PNP in S. cerevisiae was in agreement with the results of protection studies against the inactivation of the enzyme by sulfhydryl reagents, p-chloromercuribenzoate (PCMB) and 5,5'-dithiobisnitrobenzoate (DTNB). PNP was protected by ribose 1-phosphate and phosphate, but not by nucleoside or base, supporting the reaction order of ordered bi, bi mechanism. PCMB or DTNB-inactivated PNP was totally reactivated by dithiothreitol (DTT) and the activity was returned to the level of 77% by 2-mercaptoethanol, indicating that inactivation was reversible. The kinetic behavior of the PCMB-inactivated enzyme had been changed with higher
value of inosine and lower
, and was restored by DTT. Inactivation of enzyme by DTNB showed similar pattern of K sub(m) value with that by PCMB, but had not changed the
value, significantly. Negative cooperativity was not found with PCMB or DTNB treated PNP at high concentration of phosphate.
Isolation and Identification of Microorganism Producing Glutary 7-Aminodeacetoxycephalosporanis Acid Acylase
Lee, Yun-Jin ; Lim, Jai-Yun ;
The Korean Journal of Microbiology, volume 32, issue 3, 1994, Pages 232~237
Microorganism producing glutaryl 7-aminodeacetoxycephalosporanic acid (GL-7-ADCA) acylase was screened from soil. The microorganism was identified as Alcaligenes sp. J-421 by its morphology and biochemical properties. Cultural conditions of Alcaligenes sp. J-421 were investigated for the production of GL-7-ADCA acylase. Optimum medium composition was 1% glucose, 1% beef extract, 0.5% yeast extract, 0.2% monosodium L-glutamate, 0.1% glutaric acid, 0.2% NaCl, 0.5%
, and 0.05%
. Optimum cultivation conditions for the production of the enzyme in 5 l jar fermentor were
, tip speed 300 rpm, aeration 1 vvm. Optimum reaction pH of the enzyme was 8.0 and the enzyme was stable at pH7.0-11.0.
Bacterial Biomass and Secondary Productivity in Naktong River Estuary
Song, Sung-Joo ; Kwon, O-Seob ; Lee, Hye-Joo ; Lee, Jin-Ae ; Kim, Young-Eui ;
The Korean Journal of Microbiology, volume 32, issue 3, 1994, Pages 238~244
To investigate the bacterial potentials for utilizing dissolved organic matter in highly eutrophic estuary, the annual fluctuations of microbiological and physicochemical environmental parameters were analyzed in Naktong River Estuary. Total bacterial number ranged from 0.33 to
cells/ml, and correlated with the heterotrophic bacterial numbers in more eutrophic sites, especially. Bacterial biovolume and biomass varied between 0.064 and 0.156 2.09
/cell, 0.163 and 1.036
-C/ml, respectively. Bacterial secondary productivity ranged from 0.24 to 60.86
-C/l/h, and showed high correlations with the environmental parameters of pollution indicator. The seasonal variation pattern of bacterial productivity in freshwater sites was high in winter and low in summer, which was interpreted as the results of pollution loads varied with the amount of rainfall. In seawater site, the pattern was different from those of freshwater sites; high in summer and low in winter. In this site, the values of bacterial productivity showed positive correaltions with chlorophyll a, heterotrophic bacterial number, and temperature (r>0.5, p<0.05). These results suggested that the main source of organic matter which influences the bacterial productivity may be allochthonous materials in the upper freshwater zone of Naktong River Barrage, and autochthonous algal excretory products in the lower seawater zone of Naktong River Barrage.