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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Microbiology
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Journal DOI :
The Microbiological Society of Korea
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Volume & Issues
Volume 40, Issue 4 - Dec 2004
Volume 40, Issue 3 - Sep 2004
Volume 40, Issue 2 - Jun 2004
Volume 40, Issue 1 - Mar 2004
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Isolation of Tetracycline-resistant Lactic Acid Bacteria from Kimchi
The Korean Journal of Microbiology, volume 40, issue 1, 2004, Pages 1~6
Tetracycline resistant bacterial strains were isolated from 10 batches of Kimchi among 50 batches collected in Taegu restrict. The MIC of tetracycline ranged between 25 and> 100 ㎖/l. Total genomic DNA preparation from all 10 tetracycline resistant lactic acid bacterial isolates were subjected to PCR amplification with class-specific primers for tet(M) and tet(O). In only one isolate, HJ9, tet(M) was detected. By Southern blotting and hybridization with a tet(M)-specific probe, the tet(M) gene of HJ9 isolate could be localized on a plasmid. The partial nucleotide sequence and deduced amino acid sequence of tet(M) of HJ9 showed 90-99% and 94-100% homology to those of Gram positive bacteria, respectively. With sequencing of 16S rRNA, HJ9 isolate from Kimchi was identified as Lactobacillus sakei. From these results, Kimchi can be considered potential vehicle for the spread of antibiotic-resistant lactic acid bacteria along the food chain to the consumer.
Construction and Characterization of Vector Expressing Low Level of Translation Factor eIF5B
The Korean Journal of Microbiology, volume 40, issue 1, 2004, Pages 7~11
eIF5B is a translation initiation factor that delivers Met-
to AUG start codon and subsequently joins the small and large ribosomes. In order to study the function of eIF5B encoded by FUN12, we constructed FUN12 which lacked 5' end of its sequence. We found that this construct lacking almost all of its promoter in pRS plasmid partially complemented slow growth phenotype of fun12 deletion strain. Interestingly, this construct expressed N-terminally truncated eIF5B and its expression level was about 5% of that of wild type eIF5B. Low amount of the eIF5B expressed additionally in fun12 deletion strain played a direct role as a limiting factor for its growth. This limiting factor eIF5B in those strains also modulates activities of overall translation in vitro.
Cloning and Sequencing of Gene Fragment of Acid Proteinase from Penicillium oxalicum HCLF-34
The Korean Journal of Microbiology, volume 40, issue 1, 2004, Pages 12~16
Acid proteinase has been discovered in Aspergillus niger (acid protease A) and Cryphonectria parasitica (acid proteinase EapC) and it plays major roles in cheese formation from milk. In this study, a partial gene encoding acid proteinase in Penicillium oxalicum HCLF-34 was cloned by using PCR with degenerate primers corresponding to highly conserved regions of the acid proteinase. The partial acid proteinase gene in P. oxalicum HCLF-34 contains an open reading frame of 438 base pairs and encodes an acid proteinase protein of 146 amino aicds. The predicted amino acid sequences showed 71 % homology with acid protease A and 67% homology with EapC.
Antimicrobial Activities of Scutellaria baicalensis and Phellodendron amurense Against MRSA and Candida
The Korean Journal of Microbiology, volume 40, issue 1, 2004, Pages 17~22
Scutellaria baicalensis and Phellodendron amurense, which have been used traditionally in treatment of many kinds of illness including infectious diseases, were extracted with hexane, dichloromethane, methanol and water, respectively and serially. Antimicrobial activities of the extracts were examined by disk diffusion method. Methanol extract from Scutellaria baicalensis revealed high antimicrobial activities against MRSA, Gm-bacteria and Candida albicans, dichloromethnane extract from Phellodendron amurense showed lower activity than the extract of Scutellaria baicalensis. Results suggest that methanol extract from Scutellaria baicalensis could be one of the candidates for new antimicrobial agent against the antibiotic-resistant microorganisms by further steps for the purification, determination of structure and synthesis.
Immunoelectron Microscopic Localization and Analysis of Herpes simplex Virus Type 2 Antigens
The Korean Journal of Microbiology, volume 40, issue 1, 2004, Pages 23~28
Antigenic analysis of Herpes simplex type 2 virus was performed and its major antigen was localized using an immunoelectron microscopy. Antigens of 32, 43, 59 and 69 kDa were constantly expressed during the course of infection for 48 hr in the infected Vero cell. An antigen of 51 kDa was turned out to be the major one in inducing a immune response in Western-blot analysis. The 51 kDa antigen was localized on the surface of HSV-2 by immunoelectron microscopy using colloidal golds and anti-HSV 2 polyc1onal antibody. Immunofluorescence assay indicated that viral antigens were found throughout the infected cell and, especially, on the surface of the cell.
Effect of Several Physicochemical Factors on the Biodegradation of Acrylamide by Pseudomonas sp. JK-7 Isolated from Paddy Soil
The Korean Journal of Microbiology, volume 40, issue 1, 2004, Pages 29~36
The purpose of this work was to investigate the relationships between acrylamide degradation by Pseudomonas sp. JK-7 and several relevant physicochemical environment parameters. In initial experiments, the bacterial culture, strain JK-7 isolated from paddy soil sample was developed to grow aerobically with acrylamide as the sole source of carbon and nitrogen. The bacterium was identified as genus Pseudomonas in the basis of use BIOLOG test, and designated as Pseudomunas sp. JK-7. Strain JK-7 could degrade 50 mM acrylamide completely within 72 hours of incubation. Major intermediates resulting from acrylamide degradation were not detected with the HPLC methodology except acrylic acid which appeared to accumulate transiently in the growth medium. The pH increased from 7.0 to 8.7 with complete degradation of the initial 50 mM acrylamide within 72 hours of incubation. pH control in the range of 5 to 9 influenced the growth of JK-7 and acrylamide degradation, whereas it was not examined the growth and degradation at pH 3 or pH 11, respectively. The effect of supplemented carbons (e.g., glucose, fructose, citrate, succinate) on the acrylamide degradation by the test culture of JK-7 was evaluated. The results indicated that the addition of carbons accelerated the bacterial growth and acrylamide degradation compared to those in the absence of supplemented carbons. The effect of supplemented nitrogens on the degradation was monitored. Increasing concentrations of yeast extract resulted in higher growth yield, based on the turbidity measurement, and complete degradation of acrylamide. However, acrylamide degradation was essentially uninfluenced by the addition of
or urea. Addition of
in the test culture inhibited the degradation of acrylamide and growth of JK-7.
Studies on the Selective Media for Bifidobacterium infantis Maeil-K9 Using Various Carbon Sources and Antibiotics
The Korean Journal of Microbiology, volume 40, issue 1, 2004, Pages 37~42
To differentiate commercial bifidobacteia for Bifidobacterium lactis Bb-12, Bif. longum Bb-536 and Bif. infantis Maeil-K9, we studied the various carbon source, the nitrogen sources and antibiotics. Amygdalin and fructose were good candidates for carbon sources, and tryptone was suitable for nitrogen sources to design a new selective media for three commercial bifidobacteria. In the case of the amygdalin-containing medium as carbon sources, Bif. lactis Bb-12 and Bif. infantis Maeil-K9 showed good growth, and in fructose-containing medium, Bif. longum Bb-536 showed good growth. In antibiotics resistance study, the addition of 1 mg/L doxycyclin was very effective for differentiation of each bifidobacteria. Doxycyclin did not affect the growth of Bif. lactis Bb-12 and Bif. infantis Maeil-K9, but Bif. longum Bb-536 was completely inhibited by doxycyclin. Finally to confirm the selection capability of newly designed selective media, temperature-shocked bifidobacteria were cultured on them. As the results, fructose or doxycyclin containing medium showed for high growth for temperature-shocked bifidobacteria, but amygdalin containing medium showed low growth of temperature-shocked bifidobacteria.
Production of a Keratinolytic Protease by a Feather-Degrading Bacterium, Bacillus megaterium F7-1
The Korean Journal of Microbiology, volume 40, issue 1, 2004, Pages 43~48
Bacillus megaterium F7-1 producing keratinolytic protease was isolated from decayed chicken feather. The optimal culture conditions for the production of keratinolytic protease by B. megaterium F7-1 were investigated. The composition of optimal medium for the keratinolytic protease was 0.2% glucose, 0.8% skim milk, 0.05% NaCl, 0.01 %
and 0.01 %
. Especially, skim milk was found to be the most effective compound in keratinolytic protease production. The optimal temperature and initial pH were 6.5 and
, respectively. The keratinolytic protease production under optimal condition reached a maximum of 269 U/ml after 5 days of cultivation. B. megaterium F7-1 degraded 98% of the feather used in the optimized medium within 6 days.
Chungkookjang Fermentation of Mixture of Barley, Wormwood, Sea Tangle, and Soybean
The Korean Journal of Microbiology, volume 40, issue 1, 2004, Pages 49~53
Barley, wormwood, sea tangle, and soybean were fermented by Bacillus licheniformis B 1 to make Chungkookjang with better flavor and aroma. Maximal protease activity in mixed grains was observed one day after inoculation. pH increased to 8.4 two days after inoculation. Browning material derived from interaction between sugar and amino acids increased 20-fold. Thus, it is proved that Chungkookjang can be made in the mixed grains. Antioxidant activities of mixed fermented grains dissolved in ethanol or methanol (0.2 and 1 %) increased depending on their concentrations. Antioxidant activities were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH). One % of powder Chungkookjang dissolved in methanol showed highest antioxidant activity. Systolic blood pressure of hypertensive volunteers who took 20 g of mixed fermented powder decreased on average by 10 mmHg in 2 h. Preference of mixed fermented soybean containing barley, wormwood, sea tangle to fermented soybean was demonstrated by t-test analysis. Mixed fermented grains can be developed as a functional food to lower hypertension.
Effective Production of
-Glucan by the Liquid Cultivation of Agaricus blazei
The Korean Journal of Microbiology, volume 40, issue 1, 2004, Pages 54~59
-Glucan has been efficiently produced with higher yield by the optimization of liquid cultivation conditions. The optimal composition of medium for batch culture was 5% (w/v) of glucose as a carbon source, 0.5% (w/v) of yeast and 0.5% (w/v) of malt extract as a nitrogen source, 0.1% (w/v) of
and 0.05% (w/v)
, which had been the base medium for determination of other conditions. The set-up conditions are pH 5.0,
, 1 vvm for aeration and 300 rpm for agitation. In order to minimize the inhibition effect of glucose on the initial growth of mycelia and to maximize the production of extracellular
-glucan, we have reduced the initial glucose feed to 4% and added 2nd feed at the point of 70 hr from the initial feed. The 2nd feed was composed of glucose 3%, yeast extract 0.1 % and malt extract 0.1 %. It improved the
-glucan yield upto 5.2 g/L in comparison with 2.8 g/L resulted from batch cultivation. Moreover, the serial treatment of a cell wall lytic enzyme and bromelain to the mycelia was effective for extraction of the cell wall bound
-glucan. The yield of
-glucan extraction by the enzyme treatment was 3.5 g/L, which was almost 4 times higher than that by hot-water extraction.
Increased Expression of aac(3)II by Tn3 in Gentamicin - Resistant Bacteria Isolated from Hospital Sewage
The Korean Journal of Microbiology, volume 40, issue 1, 2004, Pages 60~64
We tested gentamicin - resistant bacteria isolated from hospital sewage to confirm the presence of aac(3)II encoding aminoglycoside- (3)-N- acetyltransferase by dot-blot hybridization. A probe from the internal fragment of aac(3)II was hybridized to DNA from 41 % (39/95) of gentamicin resistant isolates. PCR was performed with primers from aac(3)II and Tn3. Of 39 strains, 13 strains had Tn3-aac(3)II structure. Minimal inhibitory concentration (MIC) test demonstrated that 18 strains containing Tn3-aac(3)II showed higher resistance to gentamicin than those of other strains. Thirteen strains were identified as 5 Escherichia coli, 3 Acinetobacter johnsonii, 2 Enterobacter agglomerans, 2 Micrococcus luteus, and 1 Pseudomonas facilis. These results suggest that gentamicin-resistant determinant of Tn3-aac(3)II structure was widely distributed in the gentamicin-resistant bacteria.