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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Microbiology
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Journal DOI :
The Microbiological Society of Korea
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Volume & Issues
Volume 42, Issue 4 - Dec 2006
Volume 42, Issue 3 - Sep 2006
Volume 42, Issue 2 - Jun 2006
Volume 42, Issue 1 - Mar 2006
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Biochemical Characteristics of Lrp (Leucine-responsive Regulatory Protein) as a Global Regulator in Escherichia coli
Lee, Chan-Yong ; Kim, So-Young ; Kim, Ryu-Ryun ;
The Korean Journal of Microbiology, volume 42, issue 4, 2006, Pages 239~245
Leucine-responsive Regulatory Protein (Lrp) is a global regulator involved in modulating a variety of metabolic functions, including the catabolism and anabolism of amino acids as well as pili synthesis. In addition, there is growing evidences that Lrp may play an important role when cells make transition between rich and lean nutritional conditions. In this review, the biochemical characteristics of Lrp are described to provide a good example that shows how bacteria adapt to nutrient limitation and environmental stress.
Isolation and Characterization of the nsdC Gene in Sexual Development of Aspergillus nidulans
Kim, Hye-Ryun ; Han, Dong-Min ;
The Korean Journal of Microbiology, volume 42, issue 4, 2006, Pages 246~251
A lot of mutants which cannot initiate sexual development were screened and several loci including nsdA, nsdB, nsdC, and nsdD were identified in homothallic ascomycetes Aspergillus nidulans. The NSD206, which has nsdC6 allele, showed typical phenotype of NSD (Never in sexual development) mutants. The nsdC gene was cloned by transforming NSDP697 (
) with AMA1-NotI genomic library. The transforming library DNA recovered from several transformants showing wild phenotype carried about 10 kb genomic DNA insert. The DNA sequence of nsdC was analysed using GPS (Genome priming system). The nsdC gene has an open reading frame (ORF) of 1,929 bp encoding a putative polypeptide of 643 amino acids. The NsdC carries
type zinc finger DNA binding domains in the middle of the polypeptide. A coiled-coil domain at its C terminus were also found. In nsdC6 allele, a single T insertion was occurred between 407-408 bp leading to the frameshift mutation and early termination of translation producing the truncated protein which has only 139 amino acids.
The Trend of Antimicrobial Resistance of Escherichia coli isolated from Healthy Volunteers of Community and Hospital Patients in Incheon
Kim, Yong-Hui ; Go, Jong-Myeong ; Gong, Young-Woo ; Oh, Bo-Young ; Kim, Jung-Hee ; Kim, Hye-Young ; Lee, Mi-Yeon ; Koh, Yeon-Ja ; Hwang, Kyoung-Wha ; JeGal, Seung ; Lee, Jae-Mann ;
The Korean Journal of Microbiology, volume 42, issue 4, 2006, Pages 252~256
We monitored antibiotic resistance of Escherichia coli isolates from healthy volunteers of community and hospital patients from February to July in 2006. From disc diffusion test on 4915 E. coli isolates from healthy volunteers of the community, the resistance rates were as follows; tetracycline resistant, 46.6%; ampicillin resistant, 41.1%; ticarcillin resistant,37.9%. From disc diffusion test on 120 E. coli isolates from hospital patients, the resistance rates were as follows: ampicillin resistant, 66.9%; ticarcillin resistant, 63.8%; tetracycline resistant, 47.2%. Extended spectrum
-lactamase producing E. coli were isolated 0.6% and 4.1% from healthy volunteers and hospital patients.
Serotype and Enzymatic Profile of Crypfococcus neoformans Isolates from Clinical and Environmental Sources in Korea
Hwang, Soo-Myung ; Oh, Kwang-Seok ; Lee, Kyung-Won ;
The Korean Journal of Microbiology, volume 42, issue 4, 2006, Pages 257~264
Fifty eight Cryptococcus neoformans strains isolated from clinical and environmental sources in Korea were examined for their serotypes and extracellular enzyme activities. Among the 51 strains isolated from clinical sources, 48 strains were serotype A (94.1%), 2 strains were serotype B (3.92%), and 1 strain was serotype D (1.96%). All seven environmental strains isolated from pigeon excreta were identified as serotype A. All isolates of C. neoformtans were positive for the production of extracellular proteinase and phospholipase. In the API-ZYM system, all fifty eight isolates produced alkaine phosphatase, esterase C4, esterase lipase: C8, leucine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrase,
-glucosidase. Thirty nine isolates (67.2%) of C. neoformans produced N-acetyl-
-glucosidase. Two isolates, serotype B, and B only one serotype A produced
-glucuronidase. Analysis of enzymatic profiles to 21 enzymes revealed four biotypic patterns among the 58 strains. The enzymatic patterns of C. neoformans isolated from clinical and environmental sources represented a significant relationship with the serotypes.
Antibiotic Resistance and Assessment of the Food-borne Pathogenic Microorganisms in Ready to Eat Meals
Hong, Eun-Kyung ; Kim, Yun-A ; Lee, Do-Kyung ; Kang, Byung-Yong ; Ha, Nam-Joo ;
The Korean Journal of Microbiology, volume 42, issue 4, 2006, Pages 265~270
This study was performed in order to measure the level of food-borne pathogenic bacteria and antibiotic resistance pattern of found ready to eat meals such as Him-bap, Cho-bap, Hamburger, Sandwich and packed lunch boxes. A total of 497 samples were collected from supermarket and department of Seoul, Kyung-ki, Inctleon, Kang-won, Chung-Cheong from November, 2005 to March, 2006. The contaminated microorganisms were in most cases tract relative strain like E. coli and S. aureus. Result have shown E. coli was detected 4 strains and S. aureus was detected 22 strains. 26 strains were also tested the antibiotic resistance pattern. 26 strains were shown to be relatively susceptible to synercid, vancomycin, teicoplanin, ciprofloxacin, gentamycin, lincomycn, cefotaxime, meropenem, cephalosporin, amoxicillin-clavulanic acid by the MIC dilution method, but E. coli 1 strain was resistant to amoxicillin-clavulanic acid.
Subcellular Localization of Novel Stress Protein VISP
Moon, Chang-Hoon ; Yoon, Won-Joon ; Ko, Myoung-Seok ; Kim, Hyun-Ju ; Park, Jeong-Woo ;
The Korean Journal of Microbiology, volume 42, issue 4, 2006, Pages 271~276
Previously we demonstrated that virus-inducible stress protein (VISP) is induced in fish cells by the infection of a fish rhabdovirus. In this paper, we investigated the subcellular localization of the VISP and determined the region of VISP responsible for the subcellular localization. The CHSE-214 cells were stained with monoclonal antibody raised against VISP and observed with confocal microscope to detect the endogenous VISP. The results showed that the VISP localizes to the perinuclear region as spots. A plasmid expressing VISP fused to enhanced green fluorescent protein (EGFP) was constructed. The transient expression of full-length VISP fused to EGFP in CHSE-214 cells confirmed the spot formation of the VISP at perinuclear region. To determine the region responsible for the perinuclear localization of the VISP, we constructed a series of deletion mutants and, by using these deletion mutants, we found that C-terminal region of the VISP (aa 612-710) is essential for the perinuclear distribution of VISP and that this region contained nuclear receptor binding motif (691-TLTSLLL-697). Our results suggest that VISP localizes to the perinuclear region and C-terminal regions are important for this localization. Further studies on the role of the perinuclear localization of VISP in IHNV growth mali reveal the novel mechanism of IHNV pathogenecity.
The Analysis of Expression of Autoinducer Synthesis Genes Involved in Quorum Sensing among Catheter Associated Bacteria
Lee, Mi-Hye ; Seo, Pil-Soo ; Lee, Ji-Youl ; Peck, Kyong-Ran ; Lee, Sang-Seob ;
The Korean Journal of Microbiology, volume 42, issue 4, 2006, Pages 277~285
The most biofilm forming bacteria in catheter, Esctherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus were isolated and identified from a patient's catheter occuring catheter-associated urinary tract infection (CA-UTI). We examined mRNA expression and its quantification of AIs synthetic genes encoding signal substance of quorum sensing from each bacterial species in order to elucidated quorum sensing mechanism. Both pure cultures for each bacterial strains and a mixed cultures with three were grown for 24 hr and 30 days. Initial densities to be able to detect mRNA expression oil single strains culture were shown at
CFU/ml of E. coli for ygaG and S. aureus for luxS, and at
CFU/ml of P. aeruginosa for rhlI and lasI. Also, in mixed culture of three, initial cell densities of mRNA expression were appear to at
CFU/ml of E. coli for ygaG and S. aureus for luxS, and at
CFU/ml of P. aeruginosa for rhlI and lasI. Each AIs synthetic gene was expressed in initial cell density and the mRNA expression of the genes were detected continously during 30 days. And then, the quantification of mRNA expression level of ygaG, rhlI, last, and luxS which were related AIs synthesis was done each time point by real-time RT-PCR. Interestingly, the mRNA levels of ygaG, rhlI, lasI, and luxS from the mixed culture was higher than those from each single strain culture. In the case of E. coli ygaG, the amount of transcript from the mixed culture was at least 30 times for that from single culture. In the case of P. aeruginosa rhlI and lasI, the amount of transcript from the mixed culture was at least 40 times and 250 times for that from single strain culture. In the case of S. aureus luxS, the amount of transcript from the mixed culture was at least 5 times for that from single strain culture. And specially, the mRNA expression of rhlI and lasI of P. aeruginosa showed the highest efficency among four AIs synthetic genes.
Characteristics of Biosurfactant Producing Pseudomonas sp. G314
Shim, So-Hee ; Park, Kyeong-Ryang ;
The Korean Journal of Microbiology, volume 42, issue 4, 2006, Pages 286~293
Three hundred thirty two bacterial colonies which were able to degrade crude oil were isolated from soil samples that were contaminated with oil in Daejon area. Among them, one bacterial strain was selected for this study based on its low surface tension ability, and this selected bacterial strain was identified as Pseudomonas sp. G314 through physiological-biochemical tests and analysis of its 16S rRNA sequence. Pseudomonas sp. G314 showed a high resistance to antibiotics such as ampicillin, chloramphenicol, spectinomycin, and streptomycin, and heavy metals such as Li, Cr, and Mn. It was found that the optimal pH and temperature for biosurfactant production of Pseudomonas sp. G314 were pH 7.0 and
, respectively. After seven hours of inoculated, the biosurfactant activity reached the maximum, and surface tension of the culture broth was decreased from 72 to 25 dyne/cm. The crude biosurfactant was obtained from the culture broth by acid precipitation, followed by solvent extraction, evaporation and then freeze drying. The CMC (critical micelle concentration) value of the crude biosurfactant was 20 mg/L.
Purification of Streptomyces Phospholipase D by Immunoaffinity Chromatoghraphy using Peptide Antibodies
Park, In-Sun ; Kim, Young-Ah ; Jeong, Su-Jin ; Uhm, Tai-Boong ;
The Korean Journal of Microbiology, volume 42, issue 4, 2006, Pages 294~298
An immunoaffinity chromatography for the specific binding of Streptomyces somaliensis phospholipase D (PLD) that is considered as an industrially potential enzyme was developed. By using the protein structure prediction programs and the X-ray crystal structure of a Streptomyces PLD, 5 different epitopes with high antigenicity that are predicted to locate on the surface of the S. somaliensis PLD were selected and then synthesized for the preparation of antipeptide antibodies. Each purified rabbit IgG was coupled with NHS-activated Sepharose to prepare the immunoaffinity resins. After one-step purification of the culture concentrate on the antipeptide IgG-coupled Sepharose column, SDS-PAGE and the Western blot analysis of the purified samples showed that purification of PLD on the affinity columns was satisfactory, indicating that the peptide design using the structural information of Streptomyces PLDs was rational. However, the purified PLD in the solution aggregated rapidly, which resulted in poor specific activity and low purification yield.
D-Alaninepeptidase Increases the Vulnerability of Bacterial Cells to Osmotic Stress and Antibiotics
Song, Jin-Sue ; Lee, Young-Nam ;
The Korean Journal of Microbiology, volume 42, issue 4, 2006, Pages 299~305
D-Alaninepeptidase purified from Bacillus amyloliquefaciene CMB01 caused a reduction of survival of Proteus vulgaris, Klebsiella oxytoca, and Staphylococcus aureus placed under the osmotic pressure. D-Alaninepeptidase caused an increase of susceptibility of bacteria to antibiotics. An increased number of malformed cells in bacterial groups exposed to D-alaninepeptidase was observed by scanning electron microscopy. These data suggested that bacterial cells exposed to D-alaninepeptidase resulted in an increase of vulnerability of bacterial cells toward environmental stress, such as osmotic pressure and antibiotic substances.
Treatment of Food Garbage Using a Treatment Reactor and Microbial Consortium
Koh, Rae-Hyun ; Lee, Kang-Hyoung ; Yoo, Jin-Soo ; Song, Hong-Gyu ;
The Korean Journal of Microbiology, volume 42, issue 4, 2006, Pages 306~312
Disposal of food garbage in most large cities is very troublesome task. To date, microbiological treatment has been received an attention as a garbage decomposition process. In this study, the inoculation effect of some cellulase, amylase and protease-producing bacteria and photosynthetic bacteria on food garbage treatment was examined. They were added into a treatment reactor specially designed in this study together with food garbage and incubated in various conditions for 15 days and the removals of food garbage and foul smell produced during the treatment were analyzed. Average decomposition percentages of the inoculated food garbage in treatment reactor were 11 and 18.8% under intermittent aeration (once in a day) and continuous aeration conditions (2 L/min), respectively, and these were higher than removal percentages in the corresponding uninoculated reactors,3.4 and 13.8%. Optimal pH and temperature for food garbage decomposition by inoculated bacteria were pH 7.0 and
. Maximal decomposition percentage in the inoculated food garbage was 35% under the optimal condition (pH 7,
, and continuous aeration). The malodor compounds generated from food garbage treatment such as complex foul smell and sulfur compounds were effectively reduced about 84% and 25.5%, respectively, with a biofilter composed of purple nonsulfur bacteria trapped in sponge. This decomposing capability of food garbage by these bacteria can be utilized for the rapid and efficient treatment of food garbage.
Cloning and Expression of A Bacillus licheniformis Cellulase Gene
Yoon, Ki-Hong ;
The Korean Journal of Microbiology, volume 42, issue 4, 2006, Pages 313~318
A thermophilic bacterium producing the extracellular cellulase was isolated from soybean paste, and the isolate WL-12 has been identified as Bacillus licheniformis on the basis on its 16S rRNA sequence, morphology and biochemical properties. A gene encoding the cellulase of B. licheniformis WL-12 was cloned and its nucleotide sequence was determined. This cellulase gene, designated celA, consisted of 1,551 nucleotides, encoding a polypeptide of 517 amino acid residues. The gene product contained catalytic domain and cellulose binding domain. The deduced amino acid sequence was highly homologous to those of cellulases of B. licheniformis, B. subtilis and B. amytoliquefaciens belonging to the glycosyl hydrolase family 5. When the celA gene was highly expressed using a strong B. subtilis promoter, the extracellular cellulase was produced up to 7.0 units/ml in B. subtilis WB700.
Study on the Anti-HT-29 Human Colon Cancer Activity of
-Glucans and Their Enzymatically Hydrolyzed Oligosaccharides from Agalicus blazei Murill
Lee, Mi-Young ; Kim, Ki-Hoon ; Kim, Yea-Woon ; Chang, Hun-Gil ; Lee, Dong-Seok ;
The Korean Journal of Microbiology, volume 42, issue 4, 2006, Pages 319~325
]-Glucans (AG) were prepared from Agaricus blazei cultured in the medium fortified with the roots of Pueraria spp. by repeated extraction with hot water, gel filtration chromatography and DEAE ion exchange chromatography. Oligosaccharides (AO) were derived from the hydrolysis of AG by an endo-
6)-glucanase from Bacillus megaterium. The anti-HT-29 human colon cancer activity of AG or AO was investigated using MTT assay, apoptosis assay, cell cycle analysis, and cDNA microairay. AG and AO both inhibited proliferation and growth of HT-29 cells, and stimulated apoptosis of the cells in a dose-dependent manner. In cell cycle analysis, treating HT-29 cells with AG or AO resulted in the increase of cells in the G0 (sub-G1) and G1 phase. Especially, AO was more effective in inducing G0/G1 cell cycle arrest than AG. To screen the genes involved in the increase of apoptosis, the gene expression profile of the HT-29 cells treated with AO was examined by cDNA microarray. While several genes involved in cell cycle progression (CCND2 and CDK2) were down-regulated, many genes involved in apoptosis (TNFSF9, TNFRSF9, FADD, CASP8, BAD, CRADD, CASP9 etc), cell cycle inhibitor (CDKN2A), immune response (IL6, IL18, IL6R etc), and tumor suppressor (CEACAM1, TP53BP2, IRF1, and PHB) were up-regulated. These results suggest that AO could inhibit the proliferation and growth of HT-29 cells by G0/G1 cell cycle arrest and induction of apoptosis.
Adsorption of Heavy Metal onto the Extracellular Polysaccharide Produced by the Purple Nonsulfur Photosynthetic Bacteria Rhodopseudomonas sp. KH4
Jeong, Jeong-Hwa ; Seo, Pil-Soo ; Kong, Sung-Ho ; Lee, Jong-Yeol ; Lee, Sang-Seob ;
The Korean Journal of Microbiology, volume 42, issue 4, 2006, Pages 326~331
In the present study, we examined biosorption characteristics of heavy metals onto the extracellular polysaccharide (EPS) produced by the purple nonsulfur photosynthetic bacteria Rhodopseudomonas sp. KH4, which was isolated from a stream in Anyang, Kyonggi-Do. When Cd (100 mg/L) and Cu (100 mg/L) were added to EPS (1.0 g/L) in the optimal condition (Cd; pH 8, Cu; pH 5,
), 84.2 mg/L of Cd and 70.0 mg/L of Cu were adsorbed within 30 min and 10 min, respectively. When 100 mg/L of Cd and Cu were present as mixture, 16.8 mg/L of Cd and 48.7 mg/L of Cu were adsorbed at
, pH 5. The maximum adsorption capacity determined by fitting Langmuir isotherms model was suitable for describing the biosorption of Cd (76.9 mg/g) and Cu (67.1 mg/g) by EPS. The neutral monosaccharide in the EPS determined by GC consisted of arabinose (2.4%), glucose (7.1%) and mannose (90.5%).