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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
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Volume & Issues
Volume 43, Issue 4 - Dec 2007
Volume 43, Issue 3 - Sep 2007
Volume 43, Issue 2 - Jun 2007
Volume 43, Issue 1 - Mar 2007
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Simultaneous Detection of Cytomegalovirus, Epstein-Barr Virus, Hepatitis B Virus, and Parvovirus by a Multiplex PCR
Sung, Hye-Ran ; Joo, Jin-Young ; Lee, Chong-Kil ; Chung, Yeon-Bok ; Song, Suk-Gil ;
The Korean Journal of Microbiology, volume 43, issue 1, 2007, Pages 1~6
We describe a multiplex PCR method that can detect and differentiate simultaneously four different kinds of DNA viruses, Epstein-Barr virus (EBV), cytomegalovirus (CMV), hepatitis B virus (HBV) and parvovirus B19 (B19). Primers for the multiplex PCR reaction were designed to amplify specific regions of the EBV (pol), CMV (pol), HBV (pol) and B19 (ns) viral genomes and used to simultaneously detect individual viruses. In order to achieve optimal sensitivity and specificity for multiplex PCR, the thermo-cycling parameters, primer sequences, and concentration of each reaction components were optimized systematically. The sensitivity of the detection method ranged between 5 and 10 copies of viral genome with a mixture of multiple primer pairs. Furthermore, this highly sensitive test showed no cross-reactivity among the four viruses. Thus, the results obtained in this study provide evidence that the assay system is a good tool for supporting the diagnosis of viral infection and contamination.
Protective Antibodies and Immunity elicited by Immunization with Outer Membrane Protein H of Pasteurella multocida in Mice
Kwon, Moo-Sik ; Kim, Young-Bong ; Lee, Jeong-Min ;
The Korean Journal of Microbiology, volume 43, issue 1, 2007, Pages 7~13
Pasteurella multocida is one of the important animal pathogen causing widespread infections in various domestic animals. In swine, it causes severe respiratory diseases such as atrophic rhinitis and pneumonic pasteurellosis. To develop the efficient subunit vaccine against swine atrophic rhinitis, we investigated protective antibodies and humoral immunity of outer membrane protein H (OmpH) which is one of the major outer membrane proteins in P. multocida. Outer membrane fraction of P. multocida was immunologically detectable using antisera from both mice groups vaccinated by formalin-killed whole cells and by commercial vaccine. The expression vector for production of recombinant OmpH was constructed and the recombinant OmpH was expressed and purified from E. coli. Recombinant OmpH showed high antigenic and immunogenic properties in mice vaccination and ELISA with antisera.
Community Analysis of the Bacteria in Sponges of Lake Baikal by FISH Method
Seo, Eun-Young ; Kim, Mi-Ree ; Ahn, Tae-Seok ;
The Korean Journal of Microbiology, volume 43, issue 1, 2007, Pages 14~18
The bacterial community structures at 2 sponge species belonging to the genus Baikalospongia and Lubomirskia in Lake Baikal were analyzed with fluorescent in situ hybridization (FISH) method. The total bacterial numbers in the genus Baikalospongia ranged from
, and those in Lubomirskia from
, while those of lake water were from
. Total bacterial numbers in the sponges were
times higher than those of lake water. In the genera Baikalospongia and Lubomirskia, the proportions of other unidentified bacterial groups to the Bacteria were 42.0-60.3% and 40.7-51.9%, respectively. These proportions were similar to those in lake water (22.6-46.3%). These results suggest that bacterial compositions in Lake Baikal water and sponges are highly unique.
Purification and Characterization of Recombinant Acetohydroxyacid Synthase Catalytic Subunit in Haemophilus influenzae
Noh, Kyoung-Mi ; Choi, Kyoung-Jae ; Park, Joon-Shik ; Yoon, Moon-Young ;
The Korean Journal of Microbiology, volume 43, issue 1, 2007, Pages 19~22
Acetohydroxyacid synthase (E.C.22.214.171.124., AHAS) is the enzyme that catalyses the first step in the synthesis of the branched-chain amino acids valine, leucine and isoleucine. The AHAS gene (TIGR access code HI2585) from Heamophilus influenzae was cloned into the bacterial expression vector pET-28a and expressed in the Escherichia coli strain BL21(DE3). The expressed enzyme was purified by
HiTrap chelating HP column. The purified enzyme appears as a single band on SDS-PAGE with a molecular mass of about 63.9 kDa. The enzyme exhibits absolute dependence on the three cofactors FAD,
and thiamine diphosphate for activity. Specific activity of purified enzyme has 3.22 unit/mg and optimum activity in the pH 7.5 at
. This enzyme activity has an effect on the buffer. When comparing the enzyme activity against the organic solvent, it followed in type and the difference it is but even from the aqueous solution where the organic solvent is included with the fact that the enzyme activity is maintained.
Rapid Detection Method of Avian Influenza Subtype H5N1 using Quick Real-Time PCR
Kim, Eul-Hwan ; Lee, Dong-Woo ; Han, Sang-Hoon ; Kwon, Soon-Hwan ; Yoon, Byoung-Su ;
The Korean Journal of Microbiology, volume 43, issue 1, 2007, Pages 23~30
The most rapid Real-time PCR based detection method for Avian influenza A virus (AIV) subtype H5N1 was developed. The target DNA sequence in this study was deduced from H5N1 subtype-specific 387 bp partial gene of hemagglutinin, and was synthesized by using PCR-based gene synthesis on the ground of safety. Real-Time PCR was performed by
using microchip-based, total
of reaction mixture with extremely short time in each steps in PCR. The detection including PCR-amplication and analysis of melting temperature was totally completed within 13 min. The H5N1-specific 189 bp PCR product was correctly amplified until 2.4 molecules of hemagglutinin gene as minimum of templates. This kind of PCR was designated as Quick Real-Time PCR in this study and it could be applied to detect not only AIV H5N1, but also other pathogens using PCR-based detection.
Antimicrobial Resistance Patterns and Resistance genes assay of Shigella sonnei Isolated in Korea for Five Years
Huh, Wan ; Lee, Sang-Jo ; Kwon, Gi-Seok ; Jang, Jong-Ok ; Lee, Jung-Bok ;
The Korean Journal of Microbiology, volume 43, issue 1, 2007, Pages 31~39
This study has been carried out for investigating the relatedness of representative 135 Shigella sonnei strains isolated from 2000 to 2004 by using biotyping and antimicrobial resistance. All strains showed typical biochemical characterisics of Shigella strain. Among 135 strains,79 (58.5%) strains were biotype "g",54 (40.0%) strains were biotype "a" and 2 (1.5%) strains were biotype "e". The results of susceptibility test against 16 antimicrobial agents were like this. Most of strains were susceptible to AN, CIP, C and GM. 129 (95.6%) strains were resistant to SXT, 126 (93.3%) strains were resistant to TE and 122 (90.4%) strains were resistant to SM. One hundred thirty two (97.8%) strains were resistant to more than two antimicrobial agents. R28 type (antimicrobial resistance patterns 28: resistant to AM, SAM, TE, TIC, SXT, K, SM and AmC) were 42 strains (31.1%). The other strains were showed 33 kinds of R patterns. The results of
, sulII, tetA and strA gene detection were coincided with phenotype of antimicrobial resistance by disk diffusion method. But some strains which had sulII and strA genes were not showed the resistance against SXT and SM.
Bacterial Abundances and Enzymatic Activities in the Pore Water of Media of Artificial Floating Island in Lake Paro
Kim, Yong-Jeon ; Hur, Jai-Kyou ; Nam, Jong-Hyun ; Kim, In-Seon ; Choi, Kyoung-Suk ; Choi, Seung-Ik ; Ahn, Tae-Seok ;
The Korean Journal of Microbiology, volume 43, issue 1, 2007, Pages 40~46
For restoration of disturbed ecosystem in Lake Paro, artificial floating island (AFI) was installed. Even though the lake water was oligo-mesotrophic, the macrophytes, such as Iris ensata, Iris pseudoacorus, Phragmites communis were growing well in the rubberized coconut fiber media. For elucidating this process, total bacterial numbers, active bacterial numbers and exoenzymatic activities of
and phosphatase of pore water of media and lake water were analyzed. The average of total bacterial numbers, active bacterial numbers and exoenzymatic activities of
and phosphatase were
which were 10, 15, 22 and 38 times higher than those of lake water, respectively. Moreover, the total phosphorus and total nitrogen concentration of media showed high values of 0.82 mg/L and 7.0 mg/L, respectively, while those of lake water 0.07 mg/L and 2.3 mg/L. This results suggest that the bacteria was playing an important role for restoration of disturbed ecosystem with newly created microbial ecosystem in media of artificial floating island.
Identification and Characterization of Paenibacillus polymyxa DY1 Isolated from Korean Soil with New Antibacterial Activity
Shin, Eun-Seok ; Lee, Hee-Moo ; Lee, Bok-Kwon ; Kim, Sung-Hoon ; Kwon, Sun-Il ; Yoo, Kwan-Hee ;
The Korean Journal of Microbiology, volume 43, issue 1, 2007, Pages 47~53
The DY1 strain of Gram-positive, rod-shaped bacteria was isolated from the soil sample collected from Daeam mountain, Korea. The culture filtrate of DY1 strain showed a broad spectrum of antimicrobial activity on various pathogenic and food poisoning enteric bacterial species tested in vitro. It showed significant growth-inhibitory effect on Salmonella enterica sp., Shigella sp., pathogenic Escherichia coli, Vibrio cholerae, Vibrio parahemolyticus, and Yersinia enterocolitica. For the identification of the DY1 strain, morphological, biochemical and molecular phylogenetic approaches were performed. The DY1 strain was found to be a member of the genus Paenibacillus on the basis of morphological and biochemical analyses. The 16S rDNA of DY1 showed the highest pairwise identity with Paenibacillus polymyxa with 99.79% (1,413 bp/1,416 bp). The antimicrobial entity from DY1 looked different from preciously reported ones and seems to have a great potential to be further studied as a candidate of new antibiotics to control multi-drug resistant pathogens.
Mass Analysis of Isoflavones in Chungkookjang
Yoo, Hyung-Jae ; Hwang, Jae-Sung ; Kim, Han-Bok ;
The Korean Journal of Microbiology, volume 43, issue 1, 2007, Pages 54~58
Fermented soybean, Chungkookjang contains microorganism, enzyme, and diverse bioactive compounds. Isoflavones in Chungkookjang might suppress breast and prostate cancers. Using HPLC and Mass analyses, it was found that 100% ethanol extract of Chungkookjang contains aglycone forms such as genistein, daidzein, and glycitein. 8-OH-genistein, 8-OH-daidzein were not found. There are two estrogen receptors,
might stimulate proliferation of breast and prostate cancer cells, and
might suppress their growth. Using yeast transactivation assay under the control of human ER expression, it was demonstrated that isoflavones in Chungkookjang can stimulate
Optimization of Production of Pigment from Monascus sp. in Liquid Culture
Seo, Young-Eun ; Jung, Hyuck-Jun ; Hong, Soon-Myung ; Yu, Tae-Shick ;
The Korean Journal of Microbiology, volume 43, issue 1, 2007, Pages 59~65
The optimal conditions for Monascus pigments production of Monascus sp. KM 1001, pigment overproducing mutant, in submerged culture was investigated. The optimal medium for the production of pigment from KM 1001 mutant is determined to be composed of 4% rice powder, 0.15% Bacto-peptone, 0.1% glycine, 0.01%
. On optimal conditions,10.0 g/L of the cell mass was obtained at
for 5 days. Yellow, orange and red pigment of Monascus sp. KM 1001 were produced 3.25 units, 1.59 units and 0.88 units in extracellular part, and 84.96 units, 78.84 units and 91.80 units in intracellular part, respectively.
Isolation and Identiffication of Acinetobacter koreensis sp. nov. from Jang-Baek Waterfall
Lee, Ha-Yan ; Yoo, Yong-Kyu ; Seo, Pil-Soo ; Lee, Jung-Sook ; Lee, Keun-Chul ; Lee, Sang-Seob ;
The Korean Journal of Microbiology, volume 43, issue 1, 2007, Pages 66~71
Two isolates of genus Acinetobacter were obtained from Jang-Baek waterfall in North Korea. Morphological characteristics of the isolated 2 strains were Gram-negative, aerobic and rod shape bacteria. Physiological and biochemical characterization of the isolated 2 strains were some different aspect from those of type strains. 16S rDNA sequence analysis showed that the two isolates shared 99.9% sequence similarity. Strains JB10 and
were shown to belong to the Gammaproteobacteria and showed the highest levels of sequence similarity to Acinetobacter tandoii
(97.3%), Acinetobacter haemolyticus
(97.2%), Acinetobacter johnsonii
(97.2%), Acinetobacter junii
(96.7%), Acinetobacter schindleri
(97.0%) and Acinetobacter ursingii
(96.6%). The major cellular fatty acid in Acinetobacter type strains and isolated strains included
. Eventhough it was ascertained that the isolated strains were closely related to genus Acinetobacter, physiological and biochemical characteristics and the result of the isolated strains 16S rDNA analysis indicate some different aspects from those of type strains of genus Acinetohacter It is considered that the isolated JB10 (=KEMC 52-093) and
strains be new species of genus Acinetobacter. We name it as Acinetobacter koreensis sp. nov.
Identification and Functional Analysis of Proteins Interacting with Streptomyces coelicolor RNase ES
Kim, Jong-Myung ; Song, Woo-Seok ; Kim, Hyun-Lee ; Go, Ha-Young ; Lee, Kang-Seok ;
The Korean Journal of Microbiology, volume 43, issue 1, 2007, Pages 72~75
Using co-immunoprecipitation, we identified proteins interacting with Streptomyces coelicolor RNase ES, an ortholog of Escherichia coli RNase E that plays a major role in RNA decay and processing. Polyphosphate kinase and a homolog of exoribonuclease polynucleotide phosphorylase, guanosine pentaphosphate synthetase I that use inorganic phophate were co-precipitated with RNase E, indicating a possibility of S. coelicolor RNase ES to form a multiprotein complex called degradosome, which has been shown to be formed by RNase E in E. coli. Polynucleotide phophorylase proteins from these two phylogenetically distantly related bacteria species showed similar RNA cleavage action in vitro. These results imply the ability of RNase ES to form a multiprotein complex that has structurally and functionally similar to that of E. coli degradosome.