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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Microbiology
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Journal DOI :
The Microbiological Society of Korea
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Volume & Issues
Volume 47, Issue 4 - Dec 2011
Volume 47, Issue 3 - Sep 2011
Volume 47, Issue 2 - Jun 2011
Volume 47, Issue 1 - Mar 2011
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Development of a Rapid Detection Method for Pectobacterium carotovorum subsp. carotovorum Using the Loop-Mediated Isothermal Amplification (LAMP)
Kim, Jeong-Gu ; No, Ji-Na ; Park, Dong-Suk ; Yoon, Byoung-Su ;
The Korean Journal of Microbiology, volume 47, issue 2, 2011, Pages 103~109
Pectobacterium carotovorum subsp. carotovorum is the causative agent of soft rot in crops such as potato and cabbages. Loop-mediated isothermal amplification (LAMP) is a simple DNA amplification method, as well as isothermal PCR technique. In this study, a new method for the rapid detection of Pectobacterium carotovorum subsp. carotovorum was developed using LAMP that named PCC-LAMP. Based on lytic murein transglycolase gene of Pectobacterium carotovorum subsp. carotovorum, a set of four primers for LAMP was designed. The optimal PCC-LAMP reaction temperature was established at
. Under standard conditions, PCC-LAMP amplified
copies of clone PCC-pBX437 per reaction. Further, this method can also assay directly by SYBR Green I without electrophoresis. Amplification was not detected for five other bacterial species. In conclusion, PCC-LAMP may be a useful method for the detection Pectobacterium carotovorum subsp. carotovorum in the field.
Function of Dual Specificity Kinase, ScKns1, in Adhesive and Filamentous Growth of Saccharomyces cerevisiae
Park, Yun-Hee ; Yang, Ji-Min ; Yang, So-Young ; Kim, Sang-Mi ; Cho, Young-Mi ; Park, Hee-Moon ;
The Korean Journal of Microbiology, volume 47, issue 2, 2011, Pages 110~116
In the previous study with the Saccharomyces cerevisiae S288c strains, no known function of the dual specificity kinase, ScKns1, was reported because its gene deletion did not show any noticeable phenotypic changes. Recent study with fission yeast, however, revealed the involvement of the LAMMER kinase in flocculation, filamentous growth, oxidative stress, and so on. Therefore we made Sckns1-deletion mutants with the
-background, with which one can induce filamentous and adhesive growth in contrast to those of the S288c-background. The
strains of both haploid and diploid showed defect in filamentous growth under conditions for inducing the filamentous growth such as nitrogen starvation and butanol treatment. Both kinds of the deletion mutants also showed decrease in adhesive growth on agar surface. Interestingly enough the defects of the
strains were suppressed by the over-expression of each gene for the components of the MAPK signaling pathway such as STE11, STE12, and TEC1, respectively, but not by the upstream components, RAS2 and STE20, respectively. Although further investigations are required, these results indicate that the ScKns1 may act in place between the Ste20 and the Ste11 of the S. cerevisiae MAPK cascade.
Rapid Detection Method for Human Rotavirus from Vegetables by a Combination of Filtration and Integrated Cell Culture/Real-Time Reverse Transcription PCR
Hyeon, Ji-Yeon ; Chon, Jung-Whan ; Song, Kwang-Young ; Hwang, In-Gyun ; Kwak, Hyo-Sun ; Lee, Jung-Soo ; Kim, Moo-Sang ; Lee, Jung-Bok ; Seo, Kun-Ho ;
The Korean Journal of Microbiology, volume 47, issue 2, 2011, Pages 117~123
The purpose of this study was to evaluate and compare different elution and concentration methods for optimization of human rotavirus (HRV) detection method using real-time RT-PCR and cell culture techniques. The leafy vegetable samples (lettuce, Chinese cabbage) were artificially inoculated with HRV. Viruses were extracted from the vegetables by two different elution buffers, buffer A (100 mM Tris-HCl, 50 mM glycine, 3% beef extract, pH 9.5) and buffer B (250 mM Threonine, 300 mM NaCl, pH 9.5), and the extracted viruses were concentrated by filtration and PEG precipitation sequentially. To determine infectivity of the viruses, the viruses recovered from the samples were infected to the MA-104 cells, and integrated cell culture real-time RT-PCR was performed at 1, 48, 72, 96, 120, 144, 168 h post-infection (p.i.). The elution buffer A was more efficient in extracting the virus from the produce samples tested than the buffer B, 29.54% and 18.32% of recoveries, respectively. The sensitivity of real-time RT-PCR method was markedly improved when the virus was concentrated by the filtration method. When the viruses were eluted and concentrated by buffer A and filtration, respectively, the average recovery rate was approximately 51.89%. When the viruses recovered from samples were infected to MA-104 cell, infectious HRV was detected within 48 h p.i. by ICC/real-time RT-PCR, whereas cytopathic effects were not observed until 72 h p.i. The optimized detection method evaluated in this study could be useful for rapid and reliable detection of HRV in fresh produce products and applied for detection of other food-borne viruses.
The Role of Helicobacter pylori's Fur Protein in the Oxidative Stress Induced by Photodynamic Therapy
Park, Yu-Na ; Kim, Ji-Hoon ; Choi, Sung-Sook ;
The Korean Journal of Microbiology, volume 47, issue 2, 2011, Pages 124~129
The role of the ferric uptake regulator (Fur) of Helicobacter pylori in the oxidative stress was investigated in this study. A fur knockout mutant of H. pylori was constructed by replacing the fur gene with an aphA (kanamycin resistant marker) gene. Photodynamic therapy using methylene blue (MB) and 660 nm light was chosen to induce oxidative stress. The bactericidal effect of photodynamic therapy (PDT) was compared between wild type H. pylori and fur knockout mutant H. pylori. The degree of oxidative damage of DNA was confirmed using alkaline gel electrophoresis and an assay of 8-hydroxy-2-deoxyguanosine (8-OHdG). In control groups, the number of viable cells was maintained constantly during experiment. After PDT, the mutant H. pylori showed 10,000 times decreased viable cell number compared with wild type H. pylori. Depending on the exposure time of 660 nm light, the 3-fold increase in the concentration of 8-OHdG was observed in mutant H. pylori. The results of this study showed that H. pylori's Fur protein may play a role in oxidative stress induced by PDT.
Characterization of Actinomyces Isolated from Freshwater Sponges in Lake Baikal
Jung, You-Jung ; Joung, Yo-Chan ; Ahn, Tae-Seok ;
The Korean Journal of Microbiology, volume 47, issue 2, 2011, Pages 130~136
Five strains of Actinomyces were isolated from freshwater sponges, Baikalospongia and Lubomirskia, in Lake Baikal. By 16S rRNA sequencing, isolates were identified as Streptomyces griseoplanus, S. halstedii, S. violascens, S. flavovirens, and S. microflavus. Isolates had different characteristics of growth temperature, carbon utilization, enzyme activity, and cellular fatty acid composition. Optimum growth conditions of isolates were
, pH 8-9, and 0-1.5% salt concentrations. Major fatty acid compositions were anteiso-
, and iso-
. Strain ATS-BA-19 had DNase and chitinase activities and strain ATS-BA-16 had cellulase and protease activities. Colonies of strain ATS-BA-15 and ATS-BA-19 made inhibition zone of Pseudomonas aeruginosa.
Effective Biodegradation of Polyaromatic Hydrocarbons Through Pretreatment Using
-Coated Bamboo Activated Carbon and UV
Ekpeghere, Kalu I. ; Koo, Jin-Heui ; Kim, Jong-Hyang ; Lee, Byeong-Woo ; Yi, Sam-Nyung ; Kim, Yun-Hae ; Koh, Sung-Cheol ;
The Korean Journal of Microbiology, volume 47, issue 2, 2011, Pages 137~142
-coated bamboo activated carbon has been prepared and utilized under UV irradiation as a pretreatment method for an effective biodegradation of the recalcitrant polyaromatic hydrocarbons (PAHs). The anatase
was successfully coated on the bamboo activated carbon (AC) and it showed the highest photoactivity against methylene blue. In the absence of the PAHs-degrading bacteria PAHs having low molecular weight (i.e., naphthalene, acenaphthylene, acenaphthene, and fluorene) were degraded by 9.8, 76.2, 74.1, and 40.5%, respectively. Higher molecular weight PAHs, however, maintained high residual concentrations of PAHs (400-1,000
/L) after the same treatment. On the other hand, the overall concentrations of PAHs became lower than 340
/L when the pretreated PAHs were subjected to biodegradation by a PAH-degrading consortium for a week. Herein, phenanthrene, anthracene, fluoranthene, and pyrene were removed by 29.3, 61.4, 27.0, and 44.3%, respectively, indicating the facilitated potential biodegradation of PAHs. Activated carbon coated with
appeared to inhibit growth of PAH degraders on the surface of AC, indicating planktonic degraders were dominantly involved in the PAH biodegradation in presence of the
-coated bamboo AC. It was proposed that an effective remediation technology for the recalcitrant PAHs could be developed when an optimum pretreatment process is further established.
Making of Dongchimi Naengmyeun Broth Which Has Enhanced Antioxidant Activity Using Purple Sweet Potato
Seo, Weon-Taek ; Kim, Han-Gon ; Lee, Jin-Sung ; Cho, Kye-Man ;
The Korean Journal of Microbiology, volume 47, issue 2, 2011, Pages 143~150
Lactic fermented dongchimi naengmyeun broth which has enhanced antioxidative activity was developed. During lactic fermentation of dongchimi naengmyeun broth at
, changes of total lactic acid bacteria, pH, acidity, soluble phenolics, and anthocyanins were investigated. After 72 h of fermentation, the stronger antioxidant activities were observed in dongchimi naengmyeun broth supplemented with purple sweet potato than those of control dongchimi naengmyeun broth which showing 96.80% in DPPH radical scavenging activity, 100.82% in
scavenging activity, 7.77 in reducing power, and 6.89 in ferric reducing/antioxidant power, respectively. These high antioxidant activities related with higher contents of soluble phenolics and anthocyanins in dongchimi naengmyeun broth supplemented with purple sweet potato. The results suggest that the making of functional dongchimi naengmyeun broth by using high soluble phenolics and anthocyanins supplements such as purple sweet potato powder was possible.
Physiological Effect of Yogurt with Powder Two Stage Fermented Dioscorea batatas Dence by Monascus sp. and Lactobacillus sp.
Jeon, Chun-Pyo ; Lee, Jung-Bok ; Choi, Chung-Sig ; Kwon, Gi-Seok ;
The Korean Journal of Microbiology, volume 47, issue 2, 2011, Pages 151~157
This study was to examine the quality characteristics and biological activities of the yogurt containing dual fermentation MFCY (Monascus-Fermented Chinese Yam) powder. Six different contents (0, 0.1, 0.3, 0.5, 1.0, and 2.0%, w/v) of MFCY powder were added to raw milk and 5% skim milk followed by fermentation with lactic acid bacteria at
for 12 h, and then the biological activities of the samples were investigated. Biological activities of the prepared yogurt were evaluated for acid production (pH, titratable acidity), number of viable cells, total polyphenol contents, DPPH radical scavenging activity, reducing power, angiotensin converting enzyme inhibitory effects and GABA contents. In this study, the results show that after fermentation in raw milk and 5% skim milk added with MFCY powder. The yogurt can produced pH, titratable acidity, number of viable cells, total polyphenol contents, DPPH radical scavenging activity and reducing power at 4.12-4.25 (pH), 0.94-0.97% (TA),
CFU/ml, 161.4-329.9 (TP, mg/L), 54-94% (DPPH), and 1.13-1.53 (R.P), respectively. In addition, the showed ACE inhibitory activity and GABA contents was 67.1-87.7% and 304.6-685.4 (mg/L).
DNA Binding Specificity of Proteus mirabilis Transcription Regulator
Gang, Jong-Back ;
The Korean Journal of Microbiology, volume 47, issue 2, 2011, Pages 158~162
Amino acid sequence alignment shows that
egulator (PMTR) has cystein sequence homology at metal binding domain to CueR (copper resistance) protein, which conserves two cysteins (Cys 112 and Cys 120 in PMTR). Gel shift assay revealed that PMTR protein bound to promoter region of Escherichia coli copA (copper-translocating P-type ATPase) and Proteus mirabilis atpase (putative copper-translocating P-type ATPase) genes except that of E. coli zntA (zinc-translocating P-type ATPase) gene. DNase I protection experiment indicated that PMTR protein protected the region over -35 box and close to -10 box. DNase I hypersensitive bases were shown at C and A bases of labeled template strand and at G and C bases of labeled non-template strand of DNA. These hypersensitive bases were appeared in other metalloregulatory proteins of MerR family, which suggests protein-induced DNA bending.
Action Pattern of Anti-Yeast Substance Originated from Rahnella aquatilis Strain AY2000
Park, Hae-Ji ; Kang, Min-Jung ; Lee, Jong-Hwan ; Kim, Kwang-Hyeon ;
The Korean Journal of Microbiology, volume 47, issue 2, 2011, Pages 163~166
For reduction of side effects by anti-fungal agents, a less toxic anti-fungal substance or a synergistic substance with a new mechanism is needed. The anti-yeast substance (AYS) originated from Rahnella aquatilis strain AY2000 is like to be a heterogeneous protein. The AYS inhibited the growth of Candida albicans in culture broth, and AYS-treated cells were arrested in each phase during cell cycle. Among AYS-treated cells, the population of the cells belonging to sub-G1 phase was not increased during cell cycle. Therefore, AYS has rather yeaststatic than yeastcidal effect to C. albicans. Moreover, with combination of itraconazole or fluconazole, AYS had a synergistic anti-yeast activity against Saccharomyces cerevisiae based on the analysis of fractional inhibitory concentration index.
Temperature Sensitivity of Sigma Background Is Suppressed by the Disruption of ScKNS1 in Saccharomyces cerevisiae
Park, Yun-Hee ; Park, Hee-Moon ;
The Korean Journal of Microbiology, volume 47, issue 2, 2011, Pages 167~169
The Saccharomyces cerevisiae S288c strain does not show haploid and diploid filamentous growth, and biofilm formation, because it has a flo8 nonsense mutation unlike
strain which has a FLO8 gene. During the heat stress experiments to investigate the role of ScKns1, LAMMER kinase in S. cerevisiae, we found that
strain revealed heat sensitivity at
, a mild heat stress in contrast to S288c strain. We also found that the disruption of ScKns1 and the addition of sorbitol suppress heat sensitivity of
strain. These results suggest the possibility that Flo8 and ScKns1 may interact to transducer a signal for regulating heat stress through a novel signaling pathway.
Conservation of the Epothilone-Biosynthetic Genes in Sorangium cellulosum Strains
Hyun, Hye-Sook ; Youn, Jin-Kwon ; Cho, Kung-Yun ;
The Korean Journal of Microbiology, volume 47, issue 2, 2011, Pages 170~173
The epothilone biosynthetic gene cluster (epoA~F, epoK) of Sorangium cellulosum KYC3013, an epothilone producing myxobacterium isolated in Korea, was cloned. When the amino acid sequences of the encoded proteins were compared with those from S. cellulosum SMP44, S. cellulosum So ce90, and S. cellulosum So0157-2, which were isolated in other continents or country, the proteins from different strains were 97.4-99.8% identical each other. This suggested that the epothilone-biosynthetic gene clusters are well conserved in S. cellulosum strains.
Understanding of Extracellular Fumarate Induced dctA Gene Expression Profile Using GFP Reporter
Irisappan, Ganesh ; Ravikumar, Sambandam ; Kim, Joo-Han ; Hong, Soon-Ho ;
The Korean Journal of Microbiology, volume 47, issue 2, 2011, Pages 174~178
In Escherichia coli, DcuS/R two-component system controls fumarate import and utilization related gene expression. To understand the dynamic response of the bacterium DcuS/R two-component system with respect to fumarate concentrations, DcuS/R induced dctA promoter was integrated with GFP reporter protein. Expression monitoring study using recombinant strain showed that dctA promoter was upregulated with 1 mM of fumarate in M9 minimal medium.