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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Microbiology
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Journal DOI :
The Microbiological Society of Korea
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Volume & Issues
Volume 50, Issue 4 - Dec 2014
Volume 50, Issue 3 - Sep 2014
Volume 50, Issue 2 - Jun 2014
Volume 50, Issue 1 - Mar 2014
Selecting the target year
Recent Update in Fecal Microbiota Transplantation
Kim, Haejin ; Kang, Kyungmin ; Kim, Sujin ; Im, Eunok ;
The Korean Journal of Microbiology, volume 50, issue 4, 2014, Pages 265~274
DOI : 10.7845/kjm.2014.4072
Gut microbiota is a group of microorganisms that resides in the intestine and serves many important functions in human health. Using 16S ribosomal RNA sequencing analysis, a wide variety of bacteria in human gastrointestinal tract has been identified along with intriguing findings that there is a different bacterial composition among individuals. Fecal microbiota transplantation (FMT) is a procedure of stool transplantation from healthy donors to patients suffering from various diseases. Specifically, FMT is able to alter the composition of gut microbiota of recipients and therefore could be an effective treatment for the patients with gastrointestinal diseases including recurrent Clostridium difficile infection, inflammatory bowel disease, and irritable bowel syndrome. Here we review a list of human diseases related to gut microbiota disturbance and the case studies of FMT. We also summarize medicines and diagnostic tools that are under development. Therefore, gut microbiota can be a next generation's biotherapy for promotion of health and treatment of chronic diseases.
Fluorescence Characteristics of a Tryptophan Mutant of Leucine-responsive Regulatory Protein (Lrp)
Pokoo, Robert ; Lee, Eui Ho ; Lee, Chan Yong ;
The Korean Journal of Microbiology, volume 50, issue 4, 2014, Pages 275~280
DOI : 10.7845/kjm.2014.4075
Leucine-responsive Regulatory Protein (Lrp) from Escherichia coli is an 18.8 kDa protein composed of 164 amino acids. Wild type Lrp (Lrp Wt) does not possess any tryptophan amino acid which has strong intrinsic fluorescence, whereas the mutant Lrp R145W contains a single tryptophan at the position 145 in the leucine-responsive domain. To investigate the fluorescence character, the Lrp R145W and Lrp Wt proteins were purified. The fluorescence intensity of Lrp R145W is much higher than that of wild type protein, and the intensity of Lrp R145W was decreased by binding to its specific DNA designed from ilvIH operon and to L-leucine. In addition, the tryptophan fluorescence intensity of Lrp R145W was strongly quenched by addition of acrylamide even in the least amount of concentration as well as by urea. The data obtained from this study may give valuable information on the three dimensional structure of Lrp R145W.
Upregulaton of Bradykinin Receptor Mediated by Nucleoside Diphosphate Kinase and Flagellin from Pseudomonas aeruginosa
Kim, Yong-Jae ; Shin, Hee-Sung ; Jin, Shouguang ; Ha, Un-Hwan ;
The Korean Journal of Microbiology, volume 50, issue 4, 2014, Pages 281~284
DOI : 10.7845/kjm.2014.4063
Immune defense responses against Pseudomonas aeruginosa infection play an important role in maintaining homeostasis in the human body. Previously, we reported that expression of the bradykinin receptor (BR) is induced in response to P. aeruginosa infection. However, the factors responsible for the induction was uncertain. Here, we found that the type III secretion system (T3SS) is responsible for the induction of BR expression, and nucleoside diphosphate kinase (Ndk), as a novel T3SS effector, mediates the upregulation. The Ndk-mediated expression of BR was not induced by fliC mutant treatment, indicating the involvement of flagellin, one of the well-known pathogen-associated molecular patterns (PAMPs). Taken together, this study demonstrated that Ndk cooperates with flagella in the development of defense responses against P. aeruginosa infection.
Analysis of Epidemiological Characteristics, PFGE Typing and Antibiotic Resistance of Pathogenic Escherichia coli Strains Isolated from Gyeonggi-do
Kim, Kyung-A ; Yong, Kum-Chan ; Jeong, Jin-A ; Huh, Jeong-Weon ; Hur, Eun-Seon ; Park, Sung-Hee ; Choi, Yun-Sook ; Yoon, Mi-Hye ; Lee, Jong-Bok ;
The Korean Journal of Microbiology, volume 50, issue 4, 2014, Pages 285~295
DOI : 10.7845/kjm.2014.4057
This study was conducted to survey the epidemiological characteristics and the isolated strains for pathogenic E. coli which was the major causative organisms for food poisoning occurred at school food services in the Gyeonggi-do area during the past three years. We investigated 19 accidents of food-borne disease outbreaks by pathogenic E. coli at school food services from 2010 to 2012. Food-borne disease outbreaks by pathogenic E. coli were usually occurred at direct management type (18 accidents, 95%) and high schools. For the seasonal factors, 13 accidents (65%) were occurred in June to September, especially the end of August and September after the summer holidays. The first patients were occurred on Wednesday (7 accidents, 37%) and Thursday (7 accidents, 37%), and they were mainly reported on Thursday (7 accidents, 37%) and Friday (5 accidents, 26%). The exposure of risk was estimated in Monday (4 accidents, 21%), Tuesday (7 accidents, 37%) and Wednesday (4 accidents, 21%), and kimchi (5 accidents, 50%) was estimated as the food of the high risk responsible for the outbreaks. 98 isolates of pathogenic E. coli consisted of PEC (50%), ETEC (34%), EAEC (15%), and EHEC (1%). The antibiotic resistance of pathogenic E. coli showed in the descending order of ampicilline (40%), nalidixic acid (37%), trimethoprim/sulfamethoxazole (24%), and tetracycline (19%). The antibiotics of second and third generation cephalosporins, cabarpenem, aminoglycosides, and second generation quinolones had antimicrobial susceptibilities and cefalotin, ampicillin/sulbactam and chloramphenicol showed medium resistance at 29%, 25%, and 6% respectively, and 70% of isolates were resistant to more than one antibiotic. By the PFGE analysis, they were classified into nine major groups and 31 profiles with 57% pattern similarity. It was very difficult to find the correlation of antimicrobial susceptibilities and genotype in the small scale-food poisoning, but the similarity of antimicrobial resistance and PFGE patterns in the large scale-food poisoning enabled the outbreaks to estimate the same pathotype of E. coli derived from identical origins.
Antiviral Properties of Probiotic Mixtures against Rotavirus in the Rat
Park, Jae Eun ; Lee, Do Kyung ; Kim, Min Ji ; Kim, Kyung Tae ; Choi, Kyung Soon ; Seo, Jae Goo ; Ha, Nam Joo ;
The Korean Journal of Microbiology, volume 50, issue 4, 2014, Pages 296~301
DOI : 10.7845/kjm.2014.4064
Rotavirus is a major cause of acute gastroenteritis in young children in developed and developing countries. The use of probiotics for the treatment of gastrointestinal diseases is both safe and easily accessible. In this study, we evaluated the anti-rotaviral activities of probiotic mixtures in a Sprague-Dawley rat. 24 litters with their dams were randomly assigned to four groups; placebo, phosphate buffered saline (PBS), and two probiotic mixture (PRO-1 and PRO-2) groups. All rats were inoculated with rotavirus at dose of 8 log plaque forming units per rat at 5 days old. Animals in the PRO-1 and PRO-2 groups were orally administered probiotic mixtures 1 or 2, respectively, at a dose of 8 log colony forming units daily during 4 days. For control purposes, placebo and PBS groups were orally administered the same amount of placebo (containing maltose and polydextrose) or PBS once daily for 4 days, respectively. Antiviral analysis was performed by real-time quantitative PCR (RT-qPCR) and observing intestinal villi. As a result, weights of small intestines were greater in the PRO-1, PRO-2 groups than in control groups. Villi were short and villous epithelial necrosis was exhibited in control groups, but these morphological changes were not observed in PRO-1, PRO-2 treated rats. RT-qPCR analysis showed that VP7 gene level of rotavirus in fecal samples and small intestinal epithelial cells were lower in the PRO-1 and PRO-2 groups. These findings suggest that probiotic mixtures may be useful probiotics for the treatment of or as alternative therapies for rotaviral gastroenteritis.
Interactions between Indole-3-acetic Acid Producing Acinetobacter sp. SW5 and Growth of Tomato Plant
Kwon, Hyeok-Do ; Song, Hong-Gyu ;
The Korean Journal of Microbiology, volume 50, issue 4, 2014, Pages 302~307
DOI : 10.7845/kjm.2014.4050
Many rhizobacteria can promote plant growth through various direct or indirect mechanisms, and their production of phytohormones such as indole-3-acetic acid (IAA) may have pronounced effects on growth and development of plants. Rhizobacterial strain isolated from rhizosphere of foxtail (Setaria viridis), Acinetobacter sp. SW5 produced 118.1 mg/L of IAA and 4.5 mg/L of gibberellin (
) in brain heart broth medium at 2 and 1 day of incubation, respectively. In a pot test the lengths of stem and root and fresh weight of the germinated tomato seedlings treated with Acinetobacter sp. SW5 significantly increased by 26.3, 33.3, and 105.3%, respectively compared to those of the uninoculated control in 12 weeks of cultivation. When the root exudate secreted from tomato seedlings was analyzed by HPLC, 3.75 ng mg tomato
of tryptophan which is an IAA precursor was detected. Acinetobacter sp. SW5 could produce
of IAA from root exudate from 8 tomato seedlings. Together with the capability of growth of Acinetobacter sp. SW5 in the tomato root exudates, this IAA secreted by bacteria might contribute to enhance the growth of tomato plants.
Comparison Analysis of Swine Gut Microbiota between Landrace and Yorkshire at Various Growth Stages
Unno, Tatsuya ;
The Korean Journal of Microbiology, volume 50, issue 4, 2014, Pages 308~312
DOI : 10.7845/kjm.2014.4078
In this study, we conducted a next generation sequencing based microbial community analysis to investigate gut microbiota of the two commercially most available swine breeds, Yorkshire and Landrace. Bacterial 16S rRNA gene was amplified from fecal DNA using universal primer sets designed for V4 regions. Our comparison analysis of the gut microbiota of the two breeds suggested that their gut microbiota changed depending on the growth stages, while the difference between the two breeds was insignificant. However, there was a limited number of genera, the abundance of which was found to be different between the breeds. Those included the genus Xylanibacter in the Yorkshire samples, which was previously reported as a fiber digesting bacteria, likely increasing energy harvesting capacity of swine. In addition, others included opportunistic pathogens mostly found in the Yorkshire samples while the Landrace samples had significantly more prevalent Clostridium_IV species that were known to play a key role in systemic immunity of hosts. While microbial community shifts was found to be associated with growth stages, the difference between the two breeds seemed to be insignificant. However, there were several bacterial genera showing differential abundance, which may affect growth of hosts.
The Effect of Microalgal Growth on Nutrient Sources Using Microalgal Small Scale Raceway Pond (SSRP) for Biodiesel Production
Kim, Dong-Ho ; Kim, Byung-Hyuk ; Choi, Jong-Eun ; Kang, Zion ; Kim, Hee-Sik ;
The Korean Journal of Microbiology, volume 50, issue 4, 2014, Pages 313~318
DOI : 10.7845/kjm.2014.4076
The world is in need of sustainable and eco-friendly energy sources such as microalgal biodiesel due to global warming and fossil fuel shortages. In this study, we compared the effectiveness of liquid fertilizer produced from swine manure and agriculture grade solid fertilizers as nutrient sources for microalgal biomass production. Mixed culture (Chlorella spp., Scenedesmus spp., Stigeoclonium spp.; CSS) was cultivated for 28 days in Small Scale Raceway Pond (SSRP) using various nutrient sources (swine manure liquid fertilizer, agricultural solid fertilizer, and mixture of these two fertilizers). Biomass and lipid productivity of fertilizer mixture were the highest at 0.8 g/L and 5.8 mg/L/day, respectively. These results indicate that the fertilizer mixture can provide microalgae necessary nutrient sources for stable biodiesel production and biomass growth. In addition, overall cost of microalgal cultivation and subsequently biodiesel production would be significantly reduced.
Characterization of Biogenic Amine-reducing Pediococcus pentosaceus Isolated from Traditionally Fermented Soybean Products
Oh, HyeonHwa ; Ryu, MyeongSeon ; Heo, Jun ; Jeon, SaeBom ; Kim, Young Sang ; Jeong, DoYoun ; Uhm, Tai-Boong ;
The Korean Journal of Microbiology, volume 50, issue 4, 2014, Pages 319~326
DOI : 10.7845/kjm.2014.4056
Two bacterial strains, named as LE17 and LE22, were isolated from traditionally fermented soybean products in order to select lactic acid bacteria for the reduction of biogenic amines and harmful bacteria. Both strains were identified as Pediococcus pentosaceus by 16S rRNA sequence analysis and additional biochemical tests. The strain LE17 reduced the amines by 13.7% for histamine and by 25.9% for tyramine, when it grew in minimal synthetic media containing 0.1% (w/v) histamine and 0.1% tyramine at
for 48 h, while the strain LE22 reduced the amines by 23.7% for histamine and by 15.7% for tyramine. Both strains also had broad inhibition spectra against pathogens. Considering their properties, they could be used as starters for industrial soybean fermentation.
Comparison of Acidic pH and Temperature Stabilities between Two Bacillus Mannanases Produced from Recombinant Escherichia coli
Jeon, Ho Jin ; Yoon, Ki-Hong ;
The Korean Journal of Microbiology, volume 50, issue 4, 2014, Pages 327~333
DOI : 10.7845/kjm.2014.4060
Two genes encoding the mannanase of Bacillus sp. YB-1401 and B. amyloliquefaciens YB-1402, which had been isolated at acidic pH as mannanase producers, were each cloned into Escherichia coli, and sequenced. Both mannanase genes consisted of 1,080 nucleotides, encoding polypeptides of 360 amino acid residues. The deduced amino acid sequences of the two mannanase genes differed by four amino acid residues different, and were highly homologous to those of mannanases belonging to the glycosyl hydrolase family 26. Comparison of two mannanases produced from recombinant E. coli indicated that His-tagged mannanase of YB-1402 (HtMAN1402) was more stable than that of YB-1401 at acidic pH and high temperature. In particular, HtMAN1402 retained more than 50% of its activity at pH 3.0 after 4 h of pre-incubation, suggesting the enzyme is a valuable candidate for use as a feed additive. In addition, thermostability of the two mannanases was found to be enhanced by
Anti-Helicobacter pylori Activity of Yogurt Fermented with Lactic Acid Bacteria from Baikkimchi
Lim, Sung-Mee ; Kim, Duck-Sool ; Ahn, Dong-Hyun ;
The Korean Journal of Microbiology, volume 50, issue 4, 2014, Pages 334~344
DOI : 10.7845/kjm.2014.4058
The objective of this study was to evaluate the microbiological and physicochemical characteristics, and the antagonistic activity against Helicobacter pylori ATCC 43504, of yogurt fermented with the lactic acid bacteria from Baikkimchi kept under cold storage. The viable cell counts, titratable acidity, viscosity, and total solid content of the yogurt were different according to the bacterial strains used for fermentation. There was no significant change (P>0.05) in the various properties of refrigerated yogurt. Among the tested strains, the strongest resistance against artificial gastric juice and bile salt was found for Lactobacillus brevis BK11 and Lactobacillus paracasei BK57. Due to high lactic acid levels obtained from these two lactic acid bacteria, yogurt may show good anti-Helicobacter effects according to the time-kill assay. In particular, yogurt fermented with L. brevis BK11 significantly reduced the number of H. pylori adhering to gastric epithelial AGS cells and the urease activity of this pathogen (P<0.05).
Viability of Probiotics in Feed under High Temperature Conditions and Their Growth Inhibitory Effect on Contaminant Microbes
Kim, Gyeom-Heon ; Yi, Kwon-Jung ; Lee, Ah-Ran ; Jang, In-Hwan ; Song, In-Geun ; Kim, Dong-Woon ; Kim, Soo-Ki ;
The Korean Journal of Microbiology, volume 50, issue 4, 2014, Pages 345~350
DOI : 10.7845/kjm.2014.4046
The aim of this study was to investigate the effect of high temperature on the viability of probiotic organisms (Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae) mixed with animal feed under controlled conditions by simulating a farm feed bin in the summer. Following inoculation of probiotics into the feed, the pH and probiotic viability were monitored during an 8-day incubation at room temperature. Sterile and non-sterile feeds displayed different patterns of pH changes, with increased pH in non-sterile feed at 2 days, but a pattern of decreasing pH at 4 days. The viabilities of S. cerevisiae and B. subtilis after mono/co-inoculation were maintained without substantial changes during the incubation, whereas L. plantarum viability tended to decline. In both non-sterile and sterile feeds, the probiotics were maintained or grew without any antagonistic effects. Probiotic viability was also tested upon a shift to high temperature (
). There was no distinct change in pH between sterile and non-sterile feeds after the temperature shift. L. plantarum and S. cerevisiae could not survive at the high temperature, whereas B. subtilis displayed normal growth, and it inhibited the growth of contaminant microbes. Fungal growth was not observed in non-sterile feed 2 days after supplementation with B. subtilis. Therefore, heat resistant B. subtilis could be safely used in feed bins to inhibit microbial contamination, even at high temperatures. The prevention of elevated temperature in feed bins is necessary for the utilization of L. plantarum and S. cerevisiae during the summer season.
Microbial Diversity in the Enrichment Cultures from the Fermented Beverage of Plant Extract Using Ribosomal RNA Sequence Analysis
Lee, Choung Kyu ; Kim, Baolo ; Kang, Young Min ; Lee, Hee Yul ; Hwang, Chung Eun ; Ahn, Min Ju ; Seo, Weon Taek ; Cho, Kye Man ;
The Korean Journal of Microbiology, volume 50, issue 4, 2014, Pages 351~359
DOI : 10.7845/kjm.2014.4034
A beverage was produced by the fermentation of mixed extracts from the various fruits, vegetables, algae, and medical herbs. The physicochemical properties of the fermented beverage of plant extracts (FBPE) and microbial diversity were analyzed in cultures enriched from FBPE using 16S and 26S rRNA gene sequence analyses. The pH, acidity,
, reducing sugar, and alcohol contents of the FBPE were determined to be the 3.48, 1.68%, 70.0, 1,026 g/L, and 3.5%, respectively. The most abundant free sugar and organic acid in the FBPE were glucose (567.83 g/L) and tartaric acid (93.68 mg/L), respectively. Lactobacillus homohiochii was the predominant species in all enriched culture samples: 100% of the species in 0B (0% sugar) and 40B (40% sugar) libraries and 95.6% of 20B library (20% sugar). Lactobacillus fructivorans was detected in the 20B library. The predominant species in the samples of enrichment cultures collected from FBPE with three different sugar concentrations were: Candida zeylanoides (45.2%) in the 0Y library (0% sugar), Candida lactis-condensi (35.7%) and C. zeylanoides (35.7%) in the 20Y library (20% sugar), and C. lactis-condensi (38.1%) in the 40Y library (40% sugar). This result may provide a useful frame of reference for further analyses of microbial population dynamics in FBPE.
Antifungal Activity of Rheum undulatum on Candida albicans by the Changes in Membrane Permeability
Lee, Heung-Shick ; Kim, Younhee ;
The Korean Journal of Microbiology, volume 50, issue 4, 2014, Pages 360~367
DOI : 10.7845/kjm.2014.4085
Candida albicans is an opportunistic and the most prevalent fungal pathogen that can cause superficial and systemic infections in immunocompromised patients. C. albicans can promote the transition from budding yeast to filamentous form, generating biofilms. Infections associated with C. albicans biofilms are frequently resistant to conventional antifungal therapy. Therefore, the development of more effective antifungal drugs related with biofilm formation is required urgently. The roots of Rheum undulatum have been used for medicinal purposes in Korea and China traditionally. The aim of present study was to evaluate the effect of R. undulatum extract upon preformed biofilms of 12 clinical C. albicans isolates and the antifungal activities. Its effect on preformed biofilms was evaluated using XTT reduction assay, and metabolic activity of all tested strains was reduced significantly (
) at 0.098 mg/ml R. undulatum. The R. undulatum extract blocked the adhesion of C. albicans biofilms to polystyrene surfaces, and damaged the cell membrane integrity of C. albicans which was analyzed by CFDA, AM, and propidium iodide double staining. It caused cell lysis which was observed by Confocal laser scanning and phase contrast microscope after propidium iodide and neutral red staining, respectively. Membrane permeability was changed as evidenced by crystal violet uptake. The data suggest that R. undulatum inhibits biofilm formation by C. albicans, which can be associated with the damage of the cell membrane integrity, the changes in the membrane permeability and the cell lysis of C. albicans.
Pseudomonas sp. G19 Alleviates Salt Stress and Promotes Growth of Chinese Cabbage
Lee, Gun Woong ; Lee, Kui-Jae ; Chae, Jong-Chan ;
The Korean Journal of Microbiology, volume 50, issue 4, 2014, Pages 368~371
DOI : 10.7845/kjm.2014.4069
A variety of abiotic stresses limit plant growth and crop productivity. Among the abiotic stress, salinity is one of the major harmful stresses to plants. Plant growth-promoting bacterium was isolated from reclaimed land soil of Kyehwa-do and identified as Pseudomonas. Pseudomonas sp. strain G19 produced
of indole acetic acid and solubilized 25% of insoluble phosphate after 36 h cultivation. Also, G19 was able to produce a protein that was structurally homologous to 1-aminocyclopropane-1-carboxylate deaminase of Pseudomonas fluorescens KACC10070 playing a role in reduction of ethylene in plant. The strain G19 increased the biomass of Chinese cabbage seedlings grown in the presence of 150 mM NaCl. The results indicated that the strain G19 promoted the growth of Chinese cabbage seedling under salinity stress through microbe-plant interactions.
Protoplast Isolation and Genetic Transformation of Polyporus brumalis
Ryu, Sun-Hwa ; Kim, Myung-Kil ;
The Korean Journal of Microbiology, volume 50, issue 4, 2014, Pages 372~375
DOI : 10.7845/kjm.2014.4070
This experiment was undertaken to investigate proper conditions for protoplast isolation and genetic transformation of the white rot fungi, Polyporus brumalis. The protoplasts were formed from mycelia at a frequency of
with 0.5% Usukizyme. The transformation vector (pHYgpt) was constructed using hygromycin resistance gene (hph) for the selectable maker. The yield was 100-160 transformants/
DNA in a transformation mediated by 40% polyethylene glycol solution with aurintricarboxylic acid, heparin and supermidine. The genomic integration of the pHYgpt was confirmed by hph-specific PCR and the expected amplified band appeared only in the transformants. These results could be an efficient tool in gene engineering of the genus polyporus.
Comparison of Bacterial Communities in Beach Sands along the East Coast of North Gyeongsang Province
Khang, Yongho ;
The Korean Journal of Microbiology, volume 50, issue 4, 2014, Pages 376~380
DOI : 10.7845/kjm.2014.4067
Marine beach sands with bacterial pathogens may cause increased outcomes of illness among beachgoers in summer. In this study, pyrosequencing of 16S ribosomal DNAs extracted from 12 beach sands was performed to understand how the environmental factors of wastewaters or human wastes affected the distribution of bacterial communities at the beach of North Gyeongsang province (Yeongdeok and Pohang counties) in the middle of October. It was found that Acidobacteria were dominantly distributed in the sands near the clean seawaters, Proteobacteria in the sands near the river waters, Cyanobacteria in the sands near the wastewaters, and Bacteroidetes in the sands near the beach park. Other phyla groups such as Actinobacteria, Chlorobi, Deferribacteres, Deinococcus-thermus, Firmicutes, Gemmatimonadetes, Nitrospirae, and/or Verrucomicrobia were distributed at low relative abundance (1-5%).
Isolation and Characterization of α-Glucosidase Inhibitor Produced by Bacillus sp. SKU31-1 Strain
Kim, Shin-Duk ;
The Korean Journal of Microbiology, volume 50, issue 4, 2014, Pages 381~383
DOI : 10.7845/kjm.2014.4081
In the course of screening for
-glucosidase inhibitor produced by microorganism, the active compound was isolated from the culture filtrate of Bacillus sp. SKU31-1 using a series of chromatography procedures. The structure of the active compound was elucidated as 5-amino-1-hydroxymethyl-1, 2, 3, 4-cyclohexanetetrol on the basis of spectroscopic evidence obtained and comparison with data from the literature. The active compound showed potent inhibitory activity against
-glucosidase with an
for maltose and 4.9 mM for sucrose. A Lineweaver-Burk plot indicated that its inhibition of
-glucosidase was competitive, with a
value of 0.15 mM.
Isolation and Identification of Pseudomonas aeruginosa in Natural Environments by International Organization for Standardization ISO/NP 16266
Lee, Siwon ; Kim, Ji Hye ; Lee, Bo-Ram ; Joo, Youn-Lee ; Choe, Byeol ; Park, Su Jeong ; Chung, Hyen-Mi ; Jheong, Weon Hwa ;
The Korean Journal of Microbiology, volume 50, issue 4, 2014, Pages 384~386
DOI : 10.7845/kjm.2014.4068
Pseudomonas aeruginosa is an opportunistic pathogen that inhabits various natural and artificial environments, such as pathogenesis, water, soil and air. They can cause serious problems, such as pathogenic infection. In this study, 220 colonies were isolated from water and soil environment that assumed to be P. aeruginosa using a membrane filter method based on International Organization for Standardization (ISO/NP 16266). Identification of the isolates was determined by physiobiochemical characteristics using newly modified ISO method which includes the resistance to 1,10 phenanthroline test. Only one of 220 presumed P. aeruginosa strains isolated from effluence water using a drain swab was determined as P. aeruginosa-positive by the ISO/NP 16266 method. Subsequently, the resistance to 1,10 phenanthroline test, which was newly proposed by ISO in 2014 and applied in this study, was considered as more precise and improvable method for identification of P. aeruginosa.