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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Microbiology
Journal Basic Information
Journal DOI :
The Microbiological Society of Korea
Editor in Chief :
Volume & Issues
Volume 9, Issue 4 - Dec 1971
Volume 9, Issue 3 - Sep 1971
Volume 9, Issue 2 - Jun 1971
Volume 9, Issue 1 - Mar 1971
Selecting the target year
Effect of cation on solute permeability of mitochondrial membrane
The Korean Journal of Microbiology, volume 9, issue 4, 1971, Pages 145~148
Mitochondria were isolated from Chlorella cells effects of cation on solute permeability of mitochondrial membrane were investigated using P
as a tracer. It was strikingly increased uptake of phosphate for NaCl, KCl, while evidently decreased phosphate uptake of mitochondrial membrane for
. This consider that uptake of monovalent cation were increased, but uptake of divalentcation were decreased for permeability of mitochondrial membrane as if the permeability of protoplasmic membrane.
Relationship between growth, respiration and permeability of chlorella cell treated with gibberellic acid
The Korean Journal of Microbiology, volume 9, issue 4, 1971, Pages 149~154
Effect pf gibberellic acid (GA) on the growth rate, respiratory activity and solute uptake of Chlorella cells were measured and their correlation were discussed. Growth rate and respiratory activity of the algal cells are enhanced considerably by very samll amount (50 ppm) of GA treatment although they are suppressed by relatively higher concentration more than 100 ppm. Phosphate uptake of the algal cells, however, decreased even though lower concentration of GA is applied. Thereforem it is inferred that the growth enhancement of the algae by GA is not due to the increase of the permeability of the algal cells but expansion growth owing to the increase of osmotic pressure caused by the increase of hydrolase activity of the algae.
A study on dextrinogenic amylase in the aspergillus niger group
The Korean Journal of Microbiology, volume 9, issue 4, 1971, Pages 155~162
A comparison of dextrinogenic amylase activities in the Asp. niger group was made with their crude and ethanol dialized enzymes before and after heating at high temperature (60-
). The results obtained are as follows ; 1. THe dextrinogenic amylase activity of crude enzymes of Asp. kawachii and Asp. foetidus was strong, but Asp. phoernicis, Asp. carbonarius and Asp.japonicus showed weak activity. The others showed medial grades of activity. 2. The ethanol dialized enzymes of Asp. kawachii, Asp. foetidus and Asp. japonicus was very sesitive to high temperature (60 or
) and their enzymatic activities were diminished greatly. The others did not show diminution of enzymatic activity at 60 or 65.deg.C, but diminished greatly at 70 or
. 3. The ethanol dialized enzymes of the Asp.niger group heated to 65.deg.C was more sensitive at pH 6.0 and 6.5 than at pH 4.5, 5.0 and 5.5. 4. Tested strains in the Asp.niger group were subdivided into 4 subgroups by their dextrinogenic amylase activities before and after heating at 60 or
. The first group showed a medial grade of activity before heating and no diminution of their enzymatic activities after heating. Asp. niger, Asp.pulverulentus, Asp. awamori and Asp. usmii were included in this group. The second group had strong enzymatic activity before heating, but diminished greatly after heating. Asp rawachii and Asp. phoenicis were included in this group. The fourth group showed very weak enzymatic activity before heating, and was inactivated easily by heating. Asp.oryzae of the Asp. flavus group showed a very strong dextrinogenic amylase activity before heating. After the heat treatment, however, its enzymatic activity was diminished greatly.
Crystallization of a-Amylase and Protease of Aspergillus oryzae from Columm Chromatography (I)
The Korean Journal of Microbiology, volume 9, issue 4, 1971, Pages 163~168
Neutral protease which was obtained from a genus of Aspergilli as the crystal form were investigated for their purification and properties. The results of biochemical and enzymatic studies for their purification and properties in this enzyme were as follows. 1) On the wheat media containing 70%-water and
, Aspergilus oryzae S.H.W. 131 is satisfactorily grown under the basic optimum conditions temperature
at relative humidity 100% for three days. 2) The enzyme solution extracted with water is successively purified through the passing on column of Asmti-177N for decolorization of it. And ion exchanger such as DEAAE Sphadex A-50 or Shepadex G-100 and fraction collector is necessary for the sepearte treatments of this enzyme. After washing it with organic solvents as aceton-EtOH, etc., it should be dried on the vacuum dryer at
) The protease activity is determined by the amounts of amino acids, tyrosine. 4) The optimum pH of neutral protease is 6.0-8.0. 5) In effectively decomposing with this neutral protease, the optimum temperature is
. 6) It is interesting that the amounts of metal ion affects the activity of neutral protease. For examples, if it were treated with manganic ion, its activity would be more effective than any other that.
Electron microscopic observations of the mycelial and tissue phase of coccidioides immitis
The Korean Journal of Microbiology, volume 9, issue 4, 1971, Pages 169~174
Ultrastructural observations of mycelial and tissue phase with dimorphic fungal pathogen Coccidioides immitis were studied by electron microscopy of thin sections. 1. In mycelial phase of C.immitis contains normal cell components such as nucleus, mitochondria, endoplamsic reticulum, intracytoplasmic membrane system, cell wall and cell membrane as observed in the other encaryotic cells. 2. In tissue phase of C. immitis was larger than mycelial phase in cell size and observed much more vacuoles than mycelial phase. 3. In the contrast of mycelial phase of C. immitis, the tissue phase of cells were observed fibril form of capsular layer.
Studies on the production of Japanese encephalitis virus vaccine
The Korean Journal of Microbiology, volume 9, issue 4, 1971, Pages 175~178
Because of the cases of Japanese Encephalitis(J.E.) were reported every year in Korea. We, Dong-A Pharmaceutical Co., Ltd., produced J.E. virus vaccine, with lower price, since 1970 in order to prevent ourselves from being infected by the disease. And inoculated the J.E. virus vaccine for the children with a great success. We are going to report several questions which brought about in producing the J.E. virus vaccine by alcohol precipitation, protamine sulfate treatment method. The results obtained were as folows ; 1) In process treated with 40% alcohol, we used to ethanol made in Germany, but it was too expensive to use it. As the result which we had studied about it, we were satisfied with J.E. virus vaccine which produced with alcohol made in Korea, and then, we treated with accurate specific gravity of 40% ethanol for the precipitation of the virus. And also, we knew that it was the best method to be treated it for 3hrs,
. 2) When we treated with protamine sulfate (0.025mg/ml), we acquired the highest potent titer, and suited into purpose for the nitrogen concentration. 3) The filtration of the purified J.E. virus vaccine, in case of millipore filter paper of large pore size was not suitable for the sterility. Therefore the pore size less than 0.8.
(AA filter paper) in millipore filter paper was very suitable. But it seemed to be important subhects that the smaller was the pore size, the lower was the potent titer.
Dissemination of plant viruses by fungi
The Korean Journal of Microbiology, volume 9, issue 4, 1971, Pages 179~188
There is a good evidence that tabacco necrosis virus, lettuce big vein virus, and tabacoo stunt virus are transmitted by Oplidium brassicae, although absolute proof in aspetic condition is lakcing. Some evidence suggests that polymyxa graminis may be involved in transmission of wheat mosaic virus. One report claims that Synchytrium endobioticum can transmit potato virus X. The cultivated mushroom, Agaricus bisporus, is known to act as a hose of a virus and is apparently involved in the spread of the virus.