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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 1, Issue 2 - Dec 1973
Volume 1, Issue 1 - Jun 1973
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Stuidies on Production of Nucleic acid Derivatives by Microorganisms (IV) -Effect of Culture Media on 5′-IMP Accumulation by Brevibacterium ammoniagenes-
Microbiology and Biotechnology Letters, volume 1, issue 2, 1973, Pages 75~78
Effects of nitrogen sources, supplement of vitamins and minerals on the accumulation of 5'-inosinic acid by an adenineless mutant of Brevibacterium ammoniagenes were examined. Maximal yield of 5'-inosinic acid was attained by the use of yeast extract. as organic nitrogen source Casamino acid supplemented with
Ca-D-pantothenate and thiamine HCl could be subsitute for it. Subsequent experiment using the defined medium showed that the concentration of these trace element in the medium affected inosinic acid accumulation markedly. And it was found that the simulataneous addition of
1). thiamine Hcl(10mg/1) and Ca-D-pantothenate (5mg/l) to the defined medium stimulated inosinic acid accumulation, from which 7mg/ml of inosinic acid was obtained.
Studies on the Substitution of raw Materials of Bean Paste and Red Pepper Paste.
Microbiology and Biotechnology Letters, volume 1, issue 2, 1973, Pages 79~87
The superior materials for substitution of bean and red pepper paste were selected through the examination of chemical compositions and the function. The following results were obtained. (1) The enzyme activities were higher in the process of koji manufacturing for brewing bean paste and red pepper paste in barley koji and in corn powder koji, but those of wheat koji were inferior (2) Corn powder was proved as the most excellent substitute, because its components and functioning were the best. (3) In the production of the paste the use of corn powder and bean with equal amount was proved as good as those product of the traditional way of the brewing. (4) By using the corn powder, the production cost can be cut down to 25％ in the production of bean paste, and to 23％ in the production of red pepper paste, comparing with those of traditional brewing products
Studies on the Inhibitory Substance of Yeast Growth. Part III. Effect of the Vitamins on the Yeaststatic Activity of Astradix-P.
Microbiology and Biotechnology Letters, volume 1, issue 2, 1973, Pages 89~92
In the previous paper the biological characteristics and some reaction mechanisms of Astradix-P, yeaststatic substance, were reported. Especially yeaststatic activity of Astradix-P was anta-gonistically inhibited by alkaline amino acids, arginine, lysine and histidine, which were added as a nitrogen source in the yeast growing medium. In this paper the effects of alkaline nitrogen containing substance and several vitamins on the yeaststatic activity were investigated. The antagonistic action of alkaline nitrogen containing substance; adenine and vitamins; thiamine, riboflavin, pyridoxin, cobalamin, nicotinic acid, folic acid, biotin, p-aminobenzoic acid, inositol, and pantothenic acid to Astradix-P were not observed, thus evidencing that the yeaststatic activity of Astradix-P was not inhibited by a alkaline nitrogen containing substance and several vitamins.
Studids on the acid stable protease from Penicillium sp. part I. Isolation of Penicillium sp. and the properties of the acid proease.
Microbiology and Biotechnology Letters, volume 1, issue 2, 1973, Pages 93~98
The acid protease was isolated from the culture broth of the Penicillum sp. grown in the wheat bran media. 'quot;The crude purification of this enzyme was carried out by extraction with distilled water and precipitated with saturated ammonium sulfate. " The activity of this enzyme was found to be very strong by Folin′s colorimetric method. The results were as follows. 1. The optimum pH of the enzyme activity was at 3.0 and its optimum temperature was 5
. 2. Although the enzyme activity to hydrolyze casein was maximal at 5
, its activity decreased rapidly by about 50％, treated at 5
for 30 min. When treated at 4
for 60 min, the enzyme activity decreased to 75％ of original value and did not decrease any more. 3. The enzyme was stable at pH 2.0 to 6.0. 4. This enzyme activity was not effected by metal ions; C
, and F
5. Also, it was not effected by treatemnt of EDTA.
Studies on the Acid stable Protease from Penicillium sp. Part II. Effect of inhibitor on the proteolytic activity of acid Protease and the Milk clotting activity.
Microbiology and Biotechnology Letters, volume 1, issue 2, 1973, Pages 99~104
A study on the active center of the acid protease from Penicillium sp. was conducted, and also the milk clotting activity of acid prorease was measured. 1. PCMB failed to influence the proteolytic activity of acid protease, indicating that a reactive sulfhydryl group is not required for the enzymatic activity. 2.
-amino caproic acid did not show any inhibitory effect on tile proteolytic activity of acid protease. 3. Also 2, 4-dinitro phenol did not show any inhibitory effect on the enzyme activity. 4. Acid protease from Penicillium sp. showed a strong milk clotting activity in the presence of Ca ion. 5. This enzyme had a strong proteolytic activity on various substrate, such as casein, denatured hemoglobin, ovalbumin, denatured bovine muscle protein, denatured percine muscle protein and denatured chicken muscle protein.
Studies on the Effect of Biotin Vitamers as a Growth Factors in the L-Glutamic Acid Fermentation
Microbiology and Biotechnology Letters, volume 1, issue 2, 1973, Pages 105~113
The effect of biotin and biotin vitamer on the fermentative production of L-glutamic acid (L-GA) by Brevibacterium flavum was studied. And results were as follows. 1) L-GA production in the medium containing 10％ Glucose was the best at the concentration of Biotin 5
/l, Desthiobiotin 5
/1, and 7,8-Diaminopelargonic acid 10
/1, respectively. 2) In the experiment using the Glucose-Acetate mixed media derided into four parts, considerable amounts of cell growth and L-GA production were observed in the mixed medium containing 2％ Glucose-Acetate. 3) In the cases of using the media containing methanol, ethanol, ethylacetate, acetic acid (free acetate), Na-acetate:NH
-acetate=2 : 1, the production of L-GA were in decreasing order as follows; Na-Acetate:NH-Acetate=2 : 1> Acetic acid (free acetate)> Ethylacetate> Ethanol＞ Methanol. 4) When biotin vitamers as growth factors were added in the medium containing Glucose or Acetate as the source of carbon, the substitution effect of Desthiobiotin was almost the same, 7,8-Diaminopelargonic acid 3 or 4 times stronger, and Bisnorbiotin has no substitution effect, compared with Biotin.
Microbiology and Biotechnology Letters, volume 1, issue 2, 1973, Pages 115~117
Microbiology and Biotechnology Letters, volume 1, issue 2, 1973, Pages 119~120