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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 10, Issue 4 - Dec 1982
Volume 10, Issue 3 - Sep 1982
Volume 10, Issue 2 - Jun 1982
Volume 10, Issue 1 - Mar 1982
Selecting the target year
Studies on the Cellulolytic Enzyme System of Rhizopus sp. G-211 Isolated from Rotting Ginseng
Microbiology and Biotechnology Letters, volume 10, issue 1, 1982, Pages 1~7
A Rhizopus sp. was selected for its strong cellulolytic activity among various strains of molds found in rotting ginseng roots. Studies were made on some properties of the cellyloiytic enzyme produced by the strain. The results obtained were summarized as follows: The optimum pH of the enzyme was 4.5 and the range of its stability to the pH was 3.0 to 7.0. The optimum temperature was 5
, while the enzyme was instantly inactivated above 6
ions increased enzyme activity and the metal ions were found to increased the ther-mostability of the enzyme. This enzyme was inhibited by sodium dodecyl sulfate and 2,4-dinitrophenol. This enzyme had a strong cellulolytic enzyme activity on various native cellulose given a sufficient reaction time. The addition of 0.5% saponin solution into reaction mixture increased the enzyme activity.
Studies on Frozen Storage of Lactic Acid Bacteria
Microbiology and Biotechnology Letters, volume 10, issue 1, 1982, Pages 9~14
Three common species of lactic acid bacteria, Lactobacillus casei, Lactobacillus bulgaricus, and Streptococus lartis were tried to store in frozen state for various periods up to four months and their stabilities during the storage were evaluated. A needle of culture suspension was transferred to 10
of 10% reconstituted skim milk and frozen immediately. After storage under said conditions the storage tubes were thawed and incubated under optimum growth temperatures for 48 hours after which plate counts and acidity determinations were made. The same incubation and analyses were repeated with organisms transferred from the previous culture tubes. No significant deterioration in both survival and acid producing activity were observed during the four month storage except that some decrease in acid forming by L. bulgaricus appeared after 60 days of storage. Among the three species tested L. casei was found to be most stable and the fast was reconfirmed with scale-upped fermentation experiments using the same organism
Production of Fungal Lipids (Part 2) Isolation of Starch Utilizing Mold and Its Optimum Compositions of Growth Media
Microbiology and Biotechnology Letters, volume 10, issue 1, 1982, Pages 15~26
A potential fungal lipid producer from starch, which was identified as Muror plumbeus, was isolated from natural sources and its optimum cultivation condition for lipid production was investigated. The Mucor plumbeus FRI 0007 showed the highest felt weight and lipid content which were 2.09
0.24g per 50
of medium and 37.43% on dry weight basis respectively after 20 days incubation on the medium containing 21% of starch as a carbon source. The urea was the best nitrogen source as compared with sodium nitrate, potassium nitrate, magnesium nitrate, ammonium nitrate and ammonium acetate and its optimum concentration was 2.14g/
, showing 2.39
0.07 g felt/50
of medium and 50.73% lipid content on dry weight basis after 25 days incubation. Besides the starch as a carbon source and urea as a nitrogen source, the Mucor plumbeus FRI 0007 utilized ZnSO
as mineral sources. However, it did not require ail the above 5 minerals in group in-dispensably for its growth and lipid accumulation. The lipid and economic coefficient of Mucor plumbeus FRI 0007 grown on the medium containing 0.44g
or 5.00g MgSO
solely were 14.96 and 15.37 and 31.12 and 26.10 which was higher than those on the medium containing the above 5 minerals.
Studies on Hydrogen Evolution by Photosynthetic Bacteria(Part 1) Isolation and Identification of the Photosynthetic Bacteria
Microbiology and Biotechnology Letters, volume 10, issue 1, 1982, Pages 27~32
Many microorganisms capable of hydrogen photoproduction were isolated from samples of mud fiats of paddy field collected in Seongnam area near Seoul. Among the 63 isolants, a strain K-13 was selected for the capability of hydrogen evolution. As the results of examinations in physiological, morphological and cultural characteristics, the strain K-13 was identified as Rhodopseudomonas gelatinosa.
Studies on the Antifungal Antibiotics Produced by a Streptomyces sp. (Part 1) Selection of the Antibiotics Producing Organism and Isolation of the Antibiotics
Microbiology and Biotechnology Letters, volume 10, issue 1, 1982, Pages 33~37
The work has been carried out for the development of antifungal antibiotics possessing curative effect in the control of sheath blight disease of rice plant. Soil samples were collected from over 1600 spots throughout the country. More than 1300 specimens which seem to be the genus Streptomyces were isolated from the soil samples. Screening procedures consist of respective processes by four steps. Those are growth inhibition test in liquid culture, paper disk method, dendroid test and green house test. 102 isolates appeared to be active against Pellicularia sasakii when all specimens isolated were examined by the first growth inhibition test. Finally a strain of Streptomyces forming strong antifungal substances against P. sasakii was selected from a soil sample of Mt. Soyo, Kyeongi Province. Antifungal substances formed by the strain were isolated and purified from the culture broth and examined for antimicrobial activities as to be specific against fungi but not active on bacterial growth.
Studies on the Antifungal Antibiotics Produced by a Streptomyces sp. (Part 2) The Occurrence of trans-Cinnamamide in Streptomyces No. 297
Microbiology and Biotechnology Letters, volume 10, issue 1, 1982, Pages 39~43
t-Cinnamamide has been isolated from the culture filtrate of the isolates, Streptomyces. sp. No.297 The identity of the compound was established by UV and IR spectra, NMR, mass spectra and by chemical reactions. Through antimicrobial activity test using a two-fold serial agar dilution mothjod, t-cinnamamide showed strong growth inhibitory activity against Pellicularia sasakii, Pyriculario oryzae and some pathogenic fungi, but not inhibitory over procaryotes tested.
Studies on the Protopectinase Produced by Verticillium sp. (Part 1) Optimum Conditions for the Protopectinase Production and Utilization
Microbiology and Biotechnology Letters, volume 10, issue 1, 1982, Pages 45~52
A fungus with the highest protopectinase productivity was selected among 205 strains isolated from the soil and identified as a Verticillium sp. The Verticillium sp. was cultivated on wheat bran and the crude extruct of its culture medium showed the highest protopectinase activity on the following conditions: 3 days of cultivation time, 27
of cultivation temperature, 1.2
/g wheat bran of water content, and reinforcement of ammonium nitrate and calcium chloride at the concentration of 0.5 and 0.02%, respectively. The optimum conditions for pectin production from Citrus peel pulp by the protopectinase were consequently obtained as follows: 20
/g of liquid volume-to-pulp weight ratio, 4
of reaction temperature, and 4 of reaction pH. The higher the enzyme concentration, the better the yield of pectin and the shorter the reaction time. Total 45.6mg of pectin/g peel was produced by 1 hour reaction at the enzyme concentration of 10.5 units/
. Molecular weight of the pectin produced by the enzyme was estimated to be about 62,000 by Smit and Bryant's method.
Primary Screening for Growth Inhibitors of L1210 Cells from Oriental Herbs.
Ryu, S.H. ; Moon, K.H. ; Pack, M.Y. ;
Microbiology and Biotechnology Letters, volume 10, issue 1, 1982, Pages 53~58
In order to obtain anticancer substances from natural products, extracts of dry herbs, which have long been used to treat cancer or canter-like diseases in oriental countries, were screened. Extracts were made with hot water and/or organic solvents. With the extracts we treated murine leukemic L1210 cells growing in Fischer's medium. After 48 hours of incubation, cells were counted and concentrations of dry extracts to achieve 50 percent inhibition of the control growth, ED
values, were determined. Among the 38 species of medicinal plants tested, water extracts of six species showed ED
values of substantially low. Further extraction with organic solvents could reduce their ED
values within the range of the NCI quality control limit. The promising species as potential sources of anti-cancer substances included Cinnamomum cassia, Citrus trifoliata, Coptis japonica, Panax ginseng, Phellodendron amurense, and Scutellaria baikalensis.
High Productivity Fermentation for Ethanol Production
Lee, Kye-Joon ;
Microbiology and Biotechnology Letters, volume 10, issue 1, 1982, Pages 59~68