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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 10, Issue 4 - Dec 1982
Volume 10, Issue 3 - Sep 1982
Volume 10, Issue 2 - Jun 1982
Volume 10, Issue 1 - Mar 1982
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Studies of the Physiological Activity of Korean Ginseng (Part 2) The effects of Ginseng Saponin on the Antimicrobial Activity of Antibiotics
Microbiology and Biotechnology Letters, volume 10, issue 3, 1982, Pages 163~169
The possible effects in vivo on the duel usage of sinseng saponin and antibiotics were studied in vitro with microorganisms. Streptomycin.sulfate, kanamycin.sulfate and gentamycin.sulfate as being an aminoglycoside-antibiotic substance showed a general synergism by the interaction of ginseng saponin and these antibiotics. But kanamycin.sulfate and gentamycin.sulfate did not show a synergism in their original antimicrobial activity against Er-winia aroideoe. Chloramphenicol as being a benzene derivative displayed an increased antimicrobial activity by the interactions of ginseng saponin and this antibiotic against Salmonella typhi, Aerobacter aerogenes and the genus Serrotia. This antibiotic also showed the decreased antimicrobial activity against Bacillus subtilis, Bacillus megaterium and Escherichia coli, but did not show an uniform antimicrobial activity against others.
Studies of the Physiological Activity of Korean Ginseng (Part 3) The effects of Ginseng Saponin on the Antimicrobial Activity and Drug-resistance of Antibiotics in Bacteria
Microbiology and Biotechnology Letters, volume 10, issue 3, 1982, Pages 171~175
An oxytetracycline as being a tetracycline-antibiotic substance displayed a general synergism in the antimicrobial activity by the interaction of ginseng saponin and antibiotics, but did not to Sarcina maginata. Penicillin G.Na as being a
-lactam-antibiotic substance displayed a synergism in the antimicrobial activities by the addition of ginseng saponin to microorganisms used, but changed the effects in the antimicrobial activity of penicillin G.Na against the genus Serratia. An antimicrobial activity by the addition of ginseng saponin to antibiotics showed a non-specificity, and it varied synergism or antagonism to Gram-positive or Gram-negative bacterium. It was assumed that a drug-resistance could be eliminated by the dual administration of ginseng saponin and antibiotics.
Studies on the Penicillinase Produced by a Streptomyces sp. (Part I). Optimal Conditions for the Penicillinase Production by Streptomyces sp. YS-40.
Microbiology and Biotechnology Letters, volume 10, issue 3, 1982, Pages 177~184
Studies were carried out to investigate the optimal culture conditions for the production of penicillinase using a strain of Streptomyces sp. isolated from soil, YS-40. Among the carbon and nitrogen sources, glucose and L-asparagine increased the peniciilinase production. The addition of M
increased the enzyme production, but depressed by F
. L-Leucine slightly increased the enzyme production but L-histidine, L-methionine depressed. Among the vitamins riboflavine, i-inositol, hesperidine, niacin-amide, biotin, folic acid, DL-
-lipoic acid increased the enzyme formation. The addition of cephradine, cephalexin, ampicillin, cloxacillin more increased the enzyme formation than that of other
-lactam antibiotics and antibiotics. Optimal pH and temperature on the enzyme formation was pH 7.0 and 28
respectively Amount of the enzyme production reached at maximum with incubation for 3 days on the optimal condition.
Studies on Penicillinase Produced by a Streptomyces sp. (Part 2) Enzymatic Characteristics of the Penicillinase Produced by Streptomyces sp. YS-40.
Microbiology and Biotechnology Letters, volume 10, issue 3, 1982, Pages 185~190
A strain of Streptomyces sp. (YS-40) which was able to produce penicillinase, was isolated from soil and the enzymatic characteristics of this enzyme were investigated. The crude enzyme was obtained with the fractionation by 80 % cold acetone. The optimal temperature and pH of this enzyme was 45
and 5.0 respectively. The stable pH range of the enzyme was between pH 5.5 and 8.0 at 37
. By heat treatment at 6
for 10 min, the remained relative activities were about 50%, 30% respectively. The activity of the enzyme was inhibited by Cu
did not affect. Among 11 chemical reagents, ethylenedi aminetetra-acetic acid disodium salt (EDTA-2Na), sodium dodecyl sulfate (SDS) and sodium fluoride inhibited the enzyme activity.
Studies on the Fungi in Stored Rice
Mheen, T.I. ; Cheigh, H.S. ; Ragunathan, A.N. ; Majumder, K.S. ;
Microbiology and Biotechnology Letters, volume 10, issue 3, 1982, Pages 191~196
In order to prevent the losses of the rice by fungal deterioration during storage, fungal contaminants were isolated and identified from the grain samples (Milyang Nr.23) stored for seven months from December, 1978 to June, 1979 in silo, flat store and Tongari. Out of thirty cultures isolated from Korean paddy and brown rice samples, twenty seven species were identified, and there are eleven species of Aspergillus (A. caespitosus, A. condidus, A. chevalieri, A fischeri, A. fumigatus, A. flavus, A. nidulans, A. oryzae, A. ruber, A. sydowii, A. versicolor), five species of Penicillium (P. atramentosum, p. chrysogenum, P. cyaneofulvum, P. nototum, P. steckii), two species of each Alternaria (Al. faesiculata, Al. grisea) and Curvalaria (C, interseminata, C. tetromea), and one species of each Trichothecium roseum, Nigrospora sphaerica, Rhizopus nigricans, Fusarium spp., Mucor spp., Helminthosporium spp., and Gliocladiopsis spp. The major types of fungi grown on the surface of paddy during storage were A. flavus and A. candidus, while A. ruber and A. sydowii appeared in brown rice samples. And also A. candidus, A. versicolor and A. glacus groups were considered as major deteriorating microorganisms in stored brown and paddy rice in Korea.
Studies on the Protopectinase Produced by Verticillium sp. (Part 2) Purification and Properties of Protopectinase from Verticillium sp.
Microbiology and Biotechnology Letters, volume 10, issue 3, 1982, Pages 197~203
The protopectinase from the culture extract of a Verticillium sp. was purified about 1000 fold by ammonium sulfate fractionation, DEAE-Sephadex treatment and Sephadex G-75 column chromatography. The purified enzyme was homogeneous on electrophoresis and its molecular weight was estimated to be 38000 by Andrew's gel filtration, method. The enzyme was almost stable under the temperature of 4
and within the pH range of 3-5. Its optimum pH and temperature were 4 and 4
, respectively. The activity was markedly inhibited by galacturonic acid. The purified enzyme was able to macerate various kinds of plant tissues, such as radish, cucumber, onion, carrot, and potato. It also reduced the viscosity of pectin solution more rapidly than that of pectic acid solution and showed no lyase or CMCase activity.
Enzyme-Linked Immunosorbent Assay (ELISA) for Detection of Clostridium botulinum Type F Toxin
Lee, Jeong-Kug ; K. H. Yang ;
Microbiology and Biotechnology Letters, volume 10, issue 3, 1982, Pages 205~209
The enzyme-linked immunosorbent assay using the so-called "double-sandwich"technique was applied to determine Clostridium botulinum type F toxin. Polystyrene tubes were coated with horse anti-type F toxin serum and then toxin sample was added. The tubes were subsequently treated with rabbit anti-type F toxin IgG and sheep anti-rabbit serum IgG-horseradish peroxidase conjugate. By this technique, about 10 mouse intraperitoneal 50% lethal doses (ip LD/50/) of type F toxin could be detected. Low back-ground reading was achieved with the use of phosphate-buffered saline containing 0.05% Tween 20 and 1% bovine serum albumin as diluents of rabbit IgG and conjugate. Addition of EDTA in the diluents of toxin increased ELISA extinction value significantly. No cross-reaction was observed with botulinum type A and B toxin, but type E toxin gave sleight cross-reaction.
Studies on the Antifungal Antibiotics Produced by a Streptomyces sp. (Part 4) The Occurrence of Tetraene Substance and Its Physiological Properties
Microbiology and Biotechnology Letters, volume 10, issue 3, 1982, Pages 211~215
Streptomyces griseorubiginosus var. soyoensis previously identified, produced two kinds of antifungal antibiotics, trans-cinnamamide and another new substance. The latter was identified to be a new substance of tetraene family by establishment of UV, IR, NMR, mass spectra and chemical reactions and rotatively named as Tetraene KM-A. Through an antimicrobial activity test using serial agar dilution method, Tetraene KM-A showed strong growth inhibitory activity against fungi and yeasts, but not against procaryotes tested. The inhibitory action of Tetraene KM-A on fungi was remarkably ineffective when some of sterols were added to the cultural media.
of the Tetrene KM-A to mice and rats by intravenous injection were 84.3 and 90.4 mg/kg respectively.
to mice by oral feeding was 1503mg/kg.
Development of Phage-resistant Mutants from Lactobacillus casei
Microbiology and Biotechnology Letters, volume 10, issue 3, 1982, Pages 217~222
A lactic starter organism, Lactobaciilus casei YIT 9018 was treated with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) to obtain phage-resistant mutants. Freshly grown cells suspended in citrate buffer were exposed to NTG of 50 g/
for 40 min. Among 88 colonies isolated eight colonies showed distinct resistance to phages isolated previously from milk plants. The eight new colonies showed character similar to the original L. casei except that they responded differently to phage of different sources and thus were designated as eight different mutants of L casei. From the phage resisting toaether with the fermentative ability equivalent to the mother organism the mutants may be considered to be used as starter cultures for fermented milk.
The Oxygen Transfer and Oxygen Uptake in Antibiotic Fermentation using Streptomyces kanamyceticus
Lee, Kye-Joon ; Moo Bae ;
Microbiology and Biotechnology Letters, volume 10, issue 3, 1982, Pages 223~226
The aim of the present study was to assess the oxygen transfer rate and oxygen uptake rate in antibiotic fermentation. As a model study, cultures of Streptomyces kanamyceticus in a complex medium were analyzed to evaluate the oxygen transfer and uptake rates using oxygen balance technique. Quantitative evidence for the effect of oxygen transfer rate on the volumetric antibiotic production was clearly demonstrated. The oxygen uptake rates and the specific oxygen requirements were significantly changed with culture time. Those phenomena were indicative of biological turnover in the antibiotic fermentation
Studies on the Fermentation of Lupinseed (Part 1) Determination of the Growth Rate of Aspergillus oryzae on Beans.
Lee, Cherl-Ho ; Oh, Sung-Hoon ; Kim, Chan-Shick ;
Microbiology and Biotechnology Letters, volume 10, issue 3, 1982, Pages 227~232
The methods determining the growth rate of mold on beans were investigated in order to compare the growth of Aspergillus oryzae on lupinseed to that on soybean. The growth of A. oryzae on cooked whole or paste form of bean substrates was evaluated by the measurements of colony diameter and hyphae length of the mold. The mold showed characteristic lag times to form the colony on different types of substrate. The growth of colony diameter was coincided with the increase in
-amino nitrogen content of the substrate when the moisture level of the substrates was similar each other. The colony diameter and the cultivation time after the lag period showed a straight line relationship, from which the growth rate was estimated. in general, lupinseed paste allowed faster growth of A. oryzae than soybean paste at the initial growth phase. The lag time to form the colony was 24.0 hrs on lupinseed paste and 44.4 hrs on soybean paste. The growth rate after colony formation was, however, 7.05 mm/day for lupinseed paste and 8.83mm/day for soybean paste, which indicated that the growth rate after the lag period was faster on soybean compared to lupinseed. The sporulation time of the mold was related to the lag time for the colony formation. The measurement of hyphae length on whole beans could be used as a simple and rapid method of estimating the growth property of mold on different substrates.11 showed that the growth of A. oryzae was partly hindered by the thick hull of the lupinseed.