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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 12, Issue 4 - Dec 1984
Volume 12, Issue 3 - 00 1984
Volume 12, Issue 2 - 00 1984
Volume 12, Issue 1 - 00 1984
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Studies on the characteristics of Rhizopus japonicus Acid protease I and II
Chung, Man-Jae ;
Microbiology and Biotechnology Letters, volume 12, issue 3, 1984, Pages 179~179
The molecular weights of Acid protease I and II from Rhizopus japonicus were estimated to be the same as 38,000 by SDS-polyacrylamide gel electrophoresis, and as 38,900 by Sephadex G-100 gel filtration. Plots of log mobility of Acid protease I and II versus polyacrylamide gel concentrations gave the parallel lines indicating that they were certainly the charge isomers. lsoelectric points of Acid protease I and II were pH 4.5 and pH 7.1, respectively. Both enzymes showed the maximum absorption spectra at 283 nm. The amino acid compsitions of Acid protease I and II were slightly different from each other, but both enzymes were found to have a considerable amount of aspartic acid whereas no histidine could be detected from their amino acid compositions.
Enological Characteristics of Korean Grapes and Quality Evaluation of their Wine
Yoo, Jin-Young ; Seog, Ho-Moon ; Shin, Dong-Hwa ; Min, Byoung-Young ;
Microbiology and Biotechnology Letters, volume 12, issue 3, 1984, Pages 185~185
Enological studies were carried out to get a few fundamental data in winemaking, in terms of sugar substitution, sugar concentration, temperature effect, optimum inoculum size and variety of grape, along with the analysis of components in musts. Temperature effect in sensory evaluatin was hardly recognized among 20,25 and
. Alcohol production rates and yields were 18.798, 19.219 and 20.163m mole.
and 1.308, 1.087 and 1.147 mole(EtOH)mole
at each temperature, respectively. To obtain grape wine of 12% (v/v)alcohol, amelioration to 22% brix and 5% inoculum could be recommended. There was no significant difference (p=0.05) in organoleptic score in taste and flavor among Neo Muscat, Niagara, and Seibell 9110. However difference in appearance between Niagara and each of Seibell 9110 and Neo Muscat (p=0.01), and between Campbell Early and Muscat Bailey A (p=0.01) were recognized. Relationship of acidity and logarithm of yeast cell number, free sugar content and cell number, and nitrogen content and cell number could expressed in a first order equation;Y=1.08X-3.26, Y=-12.44x+109.59 and Y=-17.20X+149.36, respectively. There was no significant difference in sensory scores among sucrose, glucose and high fructose corn syrup as a sugar source of amelioration.
Increase of Ultraviolet resistance by Acquisition of Drug Resistance Factor R2 (Cb. Km, St) in Pseudomonas strains
Cho, Bong-Gum ;
Microbiology and Biotechnology Letters, volume 12, issue 3, 1984, Pages 191~191
Pseudomomas 2-72 harboring
drug resistant plasmid was conjugated with PAO 38 and Ps. 33-72 strains, and the
factor was transferred to the recipient strains with the frequency of
. Although the two transconjugants (PAO 38/
C and Ps. 33-72/
C) obtained showed a little difference in their resistance to U.V.irradiation, it was found that both of them were endowed with strong resistance by acquiring the plasmid. The
factor, coexisting with
plasmid already known as a
member which has an ability to give U.V. resistance to its host cell, increased further more in the resistance of their host organism. As an investigatin in relation to DNA repair mechanism,
strain was conjugated with
, a host cell reactivatin deficient strain to transfer the
factor with the frequency of
. In this case, the U.V. resistance of the transconjugant (
) was certainly increased but still lower than that of the
strain carrying no plasmid. The fact that
factor did not endow the host cells lack of the recombination repair ability with U.V. resistance suggest that the plasmid is not carrying any genetic information corresponding to rec gene and the rec gene products of host cells were involve in the development of the
Purification and properties of Adenylate kinase from Baker's Yeast
Ki, Woo-Kyung ;
Microbiology and Biotechnology Letters, volume 12, issue 3, 1984, Pages 197~197
Adenylate kinase from baker's yeast was purified about 2,000 fold in 40% yield by DEAE-cellulose column chromatography, and stepwise elution of AMP from a column of phosphocellulose. The enzyme purified was crystallized with ammonium sulfate in a phosphate buffer as pyramid form. The adenylate kinase appeared to be a single polypeptide which do not readily associated while drying process or ultracetrifugation. The molecular weight of the enzyme was estimated about 27,000 by both sodium dodesyl sulfate electrophorisis, and gel filtrtion on sephadex-G100 column. Sedimentation equilibrium resulted in the molecular weight of the enzyme 24,000. The enzyme was labile on dilution 3-
g perml stabilized in 0.1% BSA. The substrate, AMP + ATP, ATP, and AMP protected from inactivation of the enzyme in decresing order of stability.
A Study on the Production of Single Cell Protein from Ginseng-cake extract using Saccharomyces cerevisiae
Joo, Hyun-Kyu ; Cho, Gyu-Seong ;
Microbiology and Biotechnology Letters, volume 12, issue 3, 1984, Pages 205~205
The present study was carried out to investigate the effect ginseng-cake extract on the productin of yeast protein. The yeast, saccharomyces cerevisiae, was cultured on the basic molasses medium by adding 0, 10, 20 and 50% ginseng-cake extract, respectively. The effect of ginseng-cake extract on the growth, protein content and amino acid compositions of the yeast was examined. The treatment adding 10% extract showed the highest yeast production and the best fermentative ability of the yeast among 4 treatment. In the 10% treatment yeast production and fermentative ability were increased by as much as 7% and 5 to 9% compared with those of control plot, respectively. The growth of the yeast in the treatments adding extracts was faster than in the control. The 10% treatment showed 15.7% increase of the yeast growth compared with the control. The content of crude protein in the yeast was 56.25% in the control, 63.13% in the 10% treatment, 61.88% in the 20% treatment and 58.26% in the 50% treatment. The protein content of the 10% treatment was by 6.88% than that in the control. The 10% treatment showed the highest amount of total amino acid, which was 50.64%, the 20% treatment 44.75%, the control 44.53% and the 50% treatment 44.36%. In the 10% treatment the contents of 12 amino acids were higher than in the control, and among the 12 amino acids the contents of glycine, valine, proline, alanine, methionine, isoleucine, leucine and phenylalanine were considerably increased by 22.4 to 37.5%.
Studies on the Production of
-Galactosidase by Lactobacillus sporogenes (III) -Regulation of
-Galactosidase Synthesis in Growing Cells-
Kim, Young-Man ; Lee, Jung-Chi ; Choi, Yong-Jin ; Yang, Han-Chul ;
Microbiology and Biotechnology Letters, volume 12, issue 3, 1984, Pages 213~213
The study has been made to investigate induction and repression
-galactosidase sythesis in Lactobacillus sporogenes. Lactose was more effective than other metabolizable inducers tested, and the induction rate by lactose was 7.8 units/mg. Whereas, isopropyl-
-D-thiogalactopyranoside(lPTG), a non-metabolizabel inducer, induced synthesis of about 18 times more enzyme than lactose when it was fed at the concentration of 5mM to the exponentially growing culture in the basal medium containing maltose. The highest level
-galactosidase produced was 41 units/ml in the presence of [PTG after 2] hours of culture. Glucose supplied exogenously repressed enzyme synthesis significantly regardless of the inducer employed, and caused nearly 100% repression when glucose was added at the final concentration of 0.2% (w/v). Exogenous cAMP failed to release the glucose-caused catabolite repression. In addition, the uptake of lactose was severely inhibited by glucose present when the cells were grown on a mixture of glucose and lactose. Enzyme synthesis was also unrecognizable until nearly all the glucose was utilized.
Studies on the production of pullulan by Aureobasidium pullulans
Yim, Moo-Hyun ; Son, Heung-Soo ; Chung, Nag-Hyun ; Yang, Han-Chul ;
Microbiology and Biotechnology Letters, volume 12, issue 3, 1984, Pages 219~219
Cultural condition for the production of pullulan, an extracellular polysaccharide, by Aureobasidium pullulans IFO 7757 were investigated. Teh fermentation medium giving maximum pullulan yield was found to consist of 10% soluble starch as a carbon source, 0.5% potassium phosphate dibasic as a phosphate source and 0.3% ammonium sulfate as a nitrigen source. Optimum initial pH of the medium was 6.0 and the highest pullulan excretion was observed after 96 hours of cultivation at
Studies on the Adriamycin Production using Streptomyces peucetius var. caesius YS-107
Kong, In-Soo ; Oh, Doo-Hwan ; Yu, Ju-Hyun ;
Microbiology and Biotechnology Letters, volume 12, issue 3, 1984, Pages 225~225
Microorganisms which produced adriamycin had been obtained from Streptomyces peucetius var. caesius by U.V.irradiation. Among the several mutants, Streptomyces peucetius var. caesius YS-107 was showed the highest adriamycin productivity. Adriamycin had a remarkabel bacteriocidal activity against Streptococcus pyogenes (YUFE 2204). UV-light was irradiated for 25 min. in order to obtain the mutants from Streptomyces peucetius var. caesius. A medium which consists of 7.0% glucose, 1.0%
1.4% yeast extract (pH7.0) was used for the production of adriamycin. Fermentation were performed with shake cultures at 30%C for 96hus. The purified antibiotics was identified as authentic adriamycin, compared with the TLC chromatogram, IR absorption spectra and UV spectrum of adriamycin. The productivity of Streptomyces peucetius var. caesius YS-107 was about three fold than that of wild strain.
Culture conditions of Streptococcus sp. for Streptokinase Production
Suh, Hyang ; Kim, Kil-Hyoun ; Kim, Soung-Soo ; Han, Moon-Hi ;
Microbiology and Biotechnology Letters, volume 12, issue 3, 1984, Pages 231~231
Streptokinase productin was performed with a strain of Streptococcus which was isolated from human patients. The strain was estimated as one of Lancefield group C. The Streptococcus sp. grew at rich media such as brain heart infusion like other streptococcal strain. The optimal pH and concentration of brain infusion for the streptokinase (production were pH7.4 and 6.5 percent, respectively. Maximum activity of streptokinase) was detected in exponential phase of the cel growth. It was noted that the Streptococcus sp. studied didn't produce streptodornase which is produced in
-hemolytic streptococci of Lancefield group A, "human" C, and G.
Physiological Characteristics of Thialysine Resistant Mutant of Candida utilis
Bang, Byung-Ho ; Seu, Jung-Hwn ;
Microbiology and Biotechnology Letters, volume 12, issue 3, 1984, Pages 235~235
Lysine excreting, thialysine resistant mutant, TRN-4006 physiologically exhibited various propertities. Its generation time in ferementation medium was about 230min compared to 140min for wild type. This mutant also increased ability to lysine uptake. Various conditions for lysine uptake by mutant was investigated. The rate of lysine uptake was optimal at pH 6.0. When 0.6M-
Cl and 2,4-dinitrophenol were added to assay medium, lysine uptake was not occurred until 60min. After growth in minimal proline medium, lysine uptake velocity by the cells was remarkably increased, furthermore, when small-amounts of glucose were added assay wedium, there was an immediate initiation of uptake.
Cultural Condition for Lysine Production by Thialysine Resistant Mutant of Candida utilis
Bang, Byung-Ho ; Seu, Jung-Hwn ;
Microbiology and Biotechnology Letters, volume 12, issue 3, 1984, Pages 241~241
Cultural conditions on lysine production by TRN-4006 were investigated. Maximum-productivity was observed when mutant strain was grown at pH 5-6. The optimum concentration of
was 0.8%. Glucose, sucrose, and fructose were the preferred carbon sources. When medium containing 10% glucose was used, (
served as good nitrogen source. Acetate, citrate and succinate stimulated lysine production slightly, but tartrate and oxalate rather inhibited. Natural nutrient sources tested stimulated both cel growth and lysine production. Especially, addition of 0.5% beef extract pormoted productivity by about 2 fold. Lysine titer was increased by the addition of SDS into the culture broth at the end of cultivation. A highly aerobic condition was necessary for the maximum production of lysine as well as cell growth. Any concentration of
was note effective on lysine productin and
also had no effect.
Production of NADPH for Lipogensis in Oleaginous Yeast Rhodotorula glutinis
Yoon, Suk-Hoo ; Park, Jin-Seo ; Rhee, Joon-Shick ;
Microbiology and Biotechnology Letters, volume 12, issue 3, 1984, Pages 247~247
Rhodotorula glutinis(syn.R.gracilis), a red oleaginous yeast, has been grown in nitrogen-and carbon-limited continuous cultures. Using radiorespirometric methods, the proportions of pentose cycle and glycolysis in glucose catabolism have been determined at each cultural condition. As the specific lipid production rate increased, the proportion of pentose cycle in glucose catabolism was increased under both nitrogen-and carbon-limited conditions. Specific activities of dehydrogenases in pentose cycle were not affected by the changes of specific growth rates and specific lipid production rates. Malate enzyme was not shown to be a major supplier of reducing powers in this microorganism. Reducing powers being essential for lipid biosynthesis were considered to be produced mainly from pentose cycle.
Cloning of Glucose Isomerase Gene from Streptomyces phaeochromogenes in Streptomyces lividans
Kho, Yung-Hee ;
Microbiology and Biotechnology Letters, volume 12, issue 3, 1984, Pages 253~253
I report the cloning of the glucose isomerase gene from Streptomyces phaeochromogenes(NRRL B-5333). The DNA isolated from S. phaeochromogenes producing glucose isomerase was digested with Sst I and inserted into the Sst site of pIJ 702 which carries genes for thiostrepton resistance and melanin pigmentation as markers. The recombinated plasmid (pSP107) was selected on the basis of thiostrepton resistance, losing melanin pigmentation and producing the enzyme in the mutant strain of S. lividans 66 which is not producing the glucose isomerase. The plasmid (pSP 107) isolated from the cloned strain contains an insert of approximately 1,200 base pairs and located at tyrosinase site. The glucose isomerase activity of the cloned strain, S. lividans 532S107 (pSP107), was approximately 50 fold higher than the mutant strain, and 2.5 fold higher than that of the wild type.