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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 12, Issue 4 - Dec 1984
Volume 12, Issue 3 - 00 1984
Volume 12, Issue 2 - 00 1984
Volume 12, Issue 1 - 00 1984
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Ethanol Fermentation of Raw Cassava Starch (II)
Microbiology and Biotechnology Letters, volume 12, issue 4, 1984, Pages 261~264
The optimal condition of the ethanol fermentation from raw cassava starch by simultaneous saccharification - fermentation (SSF) was studied using glucoamylase from Aspergillus sp. and a yeast strain. The rate and yield of ethanol production were optimum at pH 3.6 with shaking. The fine milling treatment was effective for both saccharification and SSF of raw cassava starch. The presaccharification at 6
for 1 hr before SSF increased the rate and yield of ethanol production, as well. To increase the ethanol concentration after fermentation the substrate concentration could be increased up to 2195 without the problem of viscosity. The use of high concentration ethanol tolerant yeast strains and high substrate concentration produced ethanol higher than 10%(W/V) after fermentation for 5 days.
Production of Pyruvic Acid by Luminescent Bacterium Beneckea sp.
Microbiology and Biotechnology Letters, volume 12, issue 4, 1984, Pages 265~270
Luminous marine bacteria which have ability toproduce pyruvic acid were isolated from fresh fishes. Among them, newly isolated bacterium BL-1980 having the highest ability to produce pyruvic acid was selected and identified as Beneckea sp. Optimal conditions for the production of pyruvic acid from glucose by the bacterium BL-1980 were investigated. At the optimal conditions, 10.6g/L of pyruvic acid was produced at the conversion ratio of 35.33%.
Effect of Carbon and Nitrogen concentration on Lipid production by Rhodotorula sp.
Microbiology and Biotechnology Letters, volume 12, issue 4, 1984, Pages 271~276
Effect of C/N ratios on the cell growth, cell lipid, cell protein and fatty acids of Rhodotorula sp. was examined. At low concentration of nitrogen in cultural medium the growth of the yeast was retarded, whereas its growth was favorable at high concentration but lipid yield was decreased due to a high content of cell protein. In order to increase lipid yield, the optimum C/N ratio obtained was 45-50 (5% glucose, 0.0414% nitrogen). During the cultivation cell protein synthesis occurred mainly in initia stage and the lipid was accumulated rapidly at the late phase. The highest content of total fatty acid was observed at the optimum C/N ratio. The higher C/N ratio was the more unsaturation degree of fatty acid decreased. It was attributed principally to a decreased oleic acid with palmitic acid increased. No significant changes occurred in content of other fatty acids.
Studies on Enzymatic Characteristic′s of Adenylate Kinase from Baker′s Yeast
;Takahisa Ohta;Hiroshi Sakai;
Microbiology and Biotechnology Letters, volume 12, issue 4, 1984, Pages 277~283
In the forward reaction (ADP formation) of the adenylate kinase from baker's yeast, dissociation constants from binary complexes are higher by a factor of about 4 times then those from at ternary complexes. In the reverse reaction, dissociation constants from the binary complexes are 2 times higher then those from the ternary complexes. The enzyme showed activities against various nucleotide triphospate in following orders; ATP 100, UTP 18, ITP 9 and GTP 5, of the necleotide monophosphate. only dAMP showed 33% activity of that AMP as phosphate acceptor. Divalent cations were required in enzyme reaction in following orders;
10 and Sn
6. AMP, as a substrate inhibitor, competitively inhibited the adenylate kinase at pH 7.2 or 8.0. Inhibition constants of the enzyme showed greater dependence on the pH of the reaction mixture, which was the lower Ki values under higher pH. Adenosine pentaphospho adenosine was competive inhibitor to the enzyme against all substrate, and it showed the same Ki values, 2.9mM. Further, PEP was competive inhibitor with respect to AMP and non-competive inhibitor with respect to MgATP. Adenylate kinase from bakers yeast was similar to mitochondrial type of animal in the contents of aianine, leucine and asparagine or asparatic acid differing from muscle type enzyme. Based on the results and observation, characteristic of yeast adenylate kinase resembled the adenylate kinase of mitochondrial type from animals. Further, difference of characteristics in adenylate kinasa depending upon the workers might be due to the difference of strain used.
Influences of Proteolytic Ability of Lactic Acid Bacteria on Acid Production and Precipitates Occurrence in Liquid Yogurt Preparation
Microbiology and Biotechnology Letters, volume 12, issue 4, 1984, Pages 285~291
In making liquid yoghurt, the influences of proteolytic ability of lactic acid bacteria on acid production and on protein stability were investigated. L. bulgaricus CH-2, L. helviticus IAM 1042 and L. jugurti 3048 showed a comparatively high proteolytic activity in milk, while L. casei YIT 9018 did not show any marked proteolysis. Starter organisms having high proteolytic ability showed more rapid growth and acid production than those having low ability in milk. The most active proteolysis occurred during logarithmic growth phase of yogurt organisms, and most of the proteolysis took place in the first 24-48 hrs of incubation. Highly proteolysed yogurts made by L. bulgaricus CH-2, L. jugurti 3048, L. helviticus IAM 1042, L. acidophilus L-54 and L. casei 3012 had low protein solubility at pH 3.5 and had much protein precipitates during storage of product, but those having little protein hydrolysates made by L. casei YIT 9018 or artificial acidification showed no precipitation during keeping.
Effect of the Red Pepper Seed Contents on the Chemical Composition of Kochujang
Microbiology and Biotechnology Letters, volume 12, issue 4, 1984, Pages 293~298
Kochujangs (red pepper pastes) were mashed with the variation of seed content in the red pepper powder, i.e. none (plot A), 10%(B), 20%(C), 40 %(D) and 50%(E), and chemical compositions and qualities of the products were analysed and compared. Contents of amino nitrogen, reducing sugar and ethanol were high in the plot A and B, whereas lower levels were detected in the plot C, D and E. Differences in the contents of moisture, crude protein, crude fiber and sodium chloride were not significant among the plots, however, the plot D and E showed higher crude oil contents and pH as compared with the others. The plot B and A showed higher acidic protease and saccharogenic amylase activity as compared with the others. Taste, flavor and color were evaluated for the products which aged for 3 months, and better results were obtained in the plot A and B than in D and E. Especially the products of D and E were inferior in color.
Confirmation of Saprophytes of Onions in Korea and Effects of Temperature, Humidity and Fumigation on Boyrytis-rot.
Microbiology and Biotechnology Letters, volume 12, issue 4, 1984, Pages 299~304
Saprophytic microorganisms of onion bulbs in Korea were confirmed, and effects of temperature, humidity and fumigation by Tetrachloro isophthalonitrile on Botrytis-rot were investigated in order to decrease storage loss of onions. Dominant saprophytes were Botrytis, Penicillium and Fusarium as molds which were all pathogens, and Erwinia and Pseudomonas as bacteria of which Pseudomonas was a non-pathogen. Botrytis-rot was most effectively suppressed by temperature. At
, the incubation days at which 50% area of one onion leaf-fragment (2.5
2.5cm) inoculated by Botrytis was rotten were 26.2 days and the rotting was delayed more by 21.8 days than at
. For humidity, the effect was pretty insignificant in contrast with temperature effect. At RH 70% and
, the incubation days at which 50% area of one onion leaf-fragment was rotten were 28.0 days and the rotting was delayed more by 1.8 days than at RH9o% and
. By fumigation, the rotting was delayed by 3.8 days at RH 70% and
. In case of slightly infected samples, temperature effect was reduced and the effects of humidity and fumigation were ignored, which implies that storage samples should be healthy.
Combination of Colony Formation and Congo Red Reaction for Detecting Intra- and Extra-Cellular Cellulolytic Activities
Lee, D. S. ; Kim, H. K. ; M. Y. Pack ;
Microbiology and Biotechnology Letters, volume 12, issue 4, 1984, Pages 305~309
A selective medium which allows growth of only cellulolytic bacteria was developed. The medium composed of 0.5% carboxymethylcellulose (CMC), 0.005% yeast extract, minerals and agar. Colony formation on this medium indicates overall activities of cellulose utilization. A subsequent test with Congo Red dye could distinguish extracellular cellulolysis from intracellular type.