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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 13, Issue 4 - Dec 1985
Volume 13, Issue 3 - Sep 1985
Volume 13, Issue 2 - Jun 1985
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단백질의 치환 크로마토그래피
Yun, Tae Ho ; Kim, In Ho ;
Microbiology and Biotechnology Letters, volume 13, issue 2, 1985, Pages 13~13
Bioremediation of Trichloroethylene-Contaminated Groundwater)
Microbiology and Biotechnology Letters, volume 13, issue 2, 1985, Pages 18~18
메탄발효를 이용한 음식물 쓰레기 소화 처리 및 바이오 가스 생산 기술
Kim, Si Uk ; Park, Sun Cheol ;
Microbiology and Biotechnology Letters, volume 13, issue 2, 1985, Pages 30~30
Mutagens에 의한 DNA Damage 및 Repair Mechanisms과 Monitoring Technology
Gu, Man Bok ; Min, Ji Ho ;
Microbiology and Biotechnology Letters, volume 13, issue 2, 1985, Pages 35~35
지하수 미생물군잠의 분자생태학적 분석
Jo, Jang Cheon ; Kim, Sang Jong ;
Microbiology and Biotechnology Letters, volume 13, issue 2, 1985, Pages 43~43
Curing Action of Antibiotic Resistant Factor in Bacillus Subtilis
Hong, Yong-Ki ; Seu, Jung-Hwn ;
Microbiology and Biotechnology Letters, volume 13, issue 2, 1985, Pages 103~107
A variety of plasmid curing agents such as sodium dodecyl sulfate, acriflavine, ethidium bromide, and mitomycin-C were used to cure Bacillus subtilis cells of streptomycin resistant factor. The drug susceptibility was increased by 0.1％ sodim dodecyl sulfate at stationary growth phase. The curing frequency was obtained highly at 4
of acriflavine, 10
of ethidium bromide, and 200
of mitomycin-C. respectively. Curing action occurred competitively for the streptomycin and terramycin resistant factors in B. subtilis.
Effect of ginseng Saponin on the fatty acid composition of the lipids of Rhodotorula glutinis.
Microbiology and Biotechnology Letters, volume 13, issue 2, 1985, Pages 109~114
This study was attempted to observe the effect of crude ginseng saponin on the cell growth, lipid contents of Rhodotolula glutinis cells and fatty acid composition of the lipids from the yeast. The results obtained were as follows; The weight of dry yeast cells was increased by the addition of ginseng saponin and was noticeably increased in the treatment containing 10
％ ginseng saponin. With the increase of ginseng saponin concentration, lipids contents in Rhodotorula glutinis were increased but it's contents were decreased at the concentration of 10
％ ginseng saponin. The crude lipids of Rhodotorula glutinis were identified to be composed of palmitic, stearic, oleic, linoleic acid. Un-saturated fatty acid contents were greater than those of saturated fatty acids. Fatty acid contents were increased in the order of oleic, linoleic, palmitic and stearic acid, but palmitic acid was increased more than linoleic acid in the lipids of cells obtained at the higher than 10
％ ginseng saponin. As the ginseng saponin concentration was increased fatty acid contents were also increased, while they were considerably decreased in case of the addition of 10
％ ginseng saponin concentration.
Immobilization and Characterization of Rifamycin B Oxidase in Cellulose Acetate Beads
Chung, Bong-Hyun ; Chang, Ho-Nam ; Han, Moon-Hi ;
Microbiology and Biotechnology Letters, volume 13, issue 2, 1985, Pages 115~118
Rifamycin B oxidase converts rifamycin B to rifamycin S using oxygen as cosubstrate. Humnicola spp. (ATCC 20620) was treated with acetone and the cell powder was immobilized with cellulose acetate. The properties of the immobilized enzyme was examined. The optimum pHs of the immobilized and the free enzymes were 7.2. The optimum temperature of the immobilized enzyme was at 50-55
, which was 5
higher than that of the free enzyme. The activities of the immobilized enzyme appeared less sensistive with respect to the changes of temperature and pH as compared to those of the free enzyme. Twenty percent of the enzyme activity was recovered when the enzyme was immobilized in 3mm beads. The storage stability was good below 4
, but the activity decreased very rapidly above 5
. The physical strength of the beads was good and was suitable as packing material in a three-phase enzyme reactor.
A Study on the Production of Aromatic Amino Acids by Escherichia coli.
Park, Young-Jin ;
Microbiology and Biotechnology Letters, volume 13, issue 2, 1985, Pages 119~127
A series of Escherichia coli mutants were exmined for ability to convert glucose and ammonium salts into phenylalanine. This enabled the biochemical changes having major. effects on phenylaianine yield, and interactions between mutations, to be identified. Changes to the common pathway of aromatic biosynthesis having a major effects include desensitization of the first enzyme (3-deoxy-D-arabinoheptulosonate synthase) to end-product inhibition, and removal of repression of enzyme synthesis. It is suggested that the 3-deoxy-D-arabino-heptulosonate synthase Phe isoenzyme has a more important effect on yield. Similarly, removal of repression and end-product inhibition on the phenylalanine terminal pathway increased yield, and changes to both common and branch pathways were synergistic. Blockage of the typrosine and tryptophan pathways had minor effects on phenylalanine yield, and a mutation affecting aramatic amino acid transport (aroP) decreased yield. With multiple-mutation strains hish specific rates of product formation (ie 0.1-0.17g phenylalanine/g cells/h) were obtained.
Isolation and Identification of the Amylolytic Yeast Hansenula and its Haploid Mutant
Microbiology and Biotechnology Letters, volume 13, issue 2, 1985, Pages 129~135
The amylolytic yeasts were isolated from natural sources. Among them, a strain FRI YO-32 was selected as one of the genetically potential microorganisms and was identified as a strain of Hansenula anomala var anomala. Genetic markers were introduced into the isolated haploid strains of the strain FRI YO-32 and Saccharomyces cerevisiae by conventional mutagenic procedures with EMS or MNNG.
Protoplast Formation of the Amylolytic Yeast and Saccharomyces cerevisiae by Snail Lytic Enzyme from Helix pomatia
Microbiology and Biotechnology Letters, volume 13, issue 2, 1985, Pages 137~144
Studies were conducted on the conditions for preparation of yeast protoplasts utilizing Hansenula anomala var. anomala FRI YO-32 as well as Saccharomyces cerevisiae KFCC 32356 and a lytic enzyme from the snail Helix pomatia. The cell wails of the strain FRI YO-32 and S cerevisiae were found to be resistant to activity of the snail lytic enzyme if they were not treated with thiol compounds. Dithiothreitol was found to be more effective than 2-mercaptoethanol, but the latter was considered to be practical. As factors influencing the formation of yeast protoplast, it was considered to be concentration and incubation time of 2-mercaptoethanol or the lytic enzyme, growth stages in yeast cultivation, initial number of yeast cells, and concentration of osmotic stabilizer (KCI). Optimum conditions for the preparation of yeast protoplasts were determined.
Regeneration of Yeast Protoplast in Hansenula anomala var. anomala and Saccharomyces cerevisiae
Microbiology and Biotechnology Letters, volume 13, issue 2, 1985, Pages 145~149
Studies were conducted on the conditions for yeast protoplast regeneration in Hansenula anomala var.anomala FRI YO-32 and Saccharomyces cerevisiae. Protoplasts lysed when suspended in hypotonic solutions of KCI, and the least degree of osmolysis was shown in the hypertonic solution containing 1.4M KCI for the strain FRI YO-32 or 0.8M KCI for S. cerevisiae. It was considered that the concentration of agrar and KCI, and protoplast plating method were the main factors influencing regeneration of yeast protoplasts. Yeast protoplasts were regenerated very favorably when embedded in the complete protoplast regeneration media containing 3％ agar as well as 0.4M KCI for the strain FRI YO-32 or 1.0M KCI for S. cerevisiae. It was shown from the relationship between protoplast formation and regeneration that the higher extent of protoplast formation, the lower extent of protoplast regeneration.
Studies on the mixed culture of Lactobacillus helveticus and Streptococcus thermophilus in milk - Growth characteristics and cultural conditions for the mixed culture -
Microbiology and Biotechnology Letters, volume 13, issue 2, 1985, Pages 151~156
To investigate the new Yoghurt starter, Lactobacillus helveticus YM-1, which was selected among 14 Lactobacillus strains, and Streptococcus thermophilus CH-1 were inoculated together in reconstituted non-fat skim milk medium and their growth characteristics and cultural conditions for the mixed culture were examined. The main results of this study were obtained as follows. The typical symbiotic growth was shown between the two strains and pH and temperature for optimal growth were 6.5 and 4
, respectively. Heat treatment of milk was most effective at 10
for 30 min. The cell-free filtrate of Lactobacillus helveticus YM-1 had stimulatory effect on Streptococcus thermophilus CH-1 but the reverse case was slightly observed. Significant difference was observed in the proteolytic activities between Lactobacillus helveticus YM-1 and Streptococcus thermophilus CH-1. The former liberated 135
free amino acid per
of cultured milk, the latter 35
Studies on the Toxic Activity of Bacillus sphaericus Spo -D1216 Asporogenic Mutant
Microbiology and Biotechnology Letters, volume 13, issue 2, 1985, Pages 157~162
The cell cultures and crude extracts of Bacillus sphaericus 1593 K-5 and its mutant Spo-Dl216 were respectively bioassayed against Culex pipiens var. pollens mosquito larvae. The B. sphaeriucs 1593 K-5 showed toxic activity against the larvae. LC
) was 2.6
. Also the LC
) of the crude extract was 10.26. However, B. sphaericus Spo-Dl216 didn't show toxic activity against the larvae. The soluble cytoplasmic toxin in broken B. sphaeriucs 1593k-5 cells was partially purified by gel permeation chromatography and ion exchange chromatography. Among the fractions of the gel permeation chromatography only a single fraction was found to be toxic. LC
) of the active fraction was 0.182. The active fraction of the gel permeation was subjected to ion exchange chromatography. Only a single fraction showed toxic activity and its LC
) was 0.02..02.
A Study on the Protoplast fusion of Streptococcus lactis
Microbiology and Biotechnology Letters, volume 13, issue 2, 1985, Pages 163~167
To investigate the condition for the protoplast fusion of Streptococcus lactis, streptomycin(200
) resistant and rifampicin (200
) resistant mutants were isolated. By using these markers, protoplast fusion was carried out in the presence of CaCl
and polyethylene glycol. The optimal conditions for the protoplast fusion were obtained by treatment of protoplasts with 150 mM CaCl
(final concentration; 25 mM) and 40％ (w/v) PEG 4, 000 for 2 min. At the optimal conditions, the fusion frequency was 6.26
. On the other hand, genetic recombination between the antibiotic resistant mutants by mating was not observed.
The Assimilability of Glucose and Xylose in Rhodopseudomonas sp. K-7.
Microbiology and Biotechnology Letters, volume 13, issue 2, 1985, Pages 169~172
The assimilability of glucose and xylose of Rhodopseudomonas K-7, whose hydrogen evolution has been characterized previously, was investigated under the anaerobic photosynthetic and the aerobic dark conditions. This organism is able to grow well in the medium containing glutamate and malate as organic substances under the anaerobic light condition. However, the substitution of glucose for malate retarded the growth rate, while the addition of glucose to the seed culture remarkably promoted the utilization of glucose added in the main culture. Optimal glucose concentration in the seed culture to induce glucose assimilability of the organism was around the concentration of 60 mM of glucose. Then, the seed culture grown in the medium containing 60 mM of glucose were inoculated in the medium containing 10, 20, 30, 60 and 100 mM of glucose respectively. The results were revealed that the consumable content of glucose was limited even though the high concentrations of glucose was contained in the medium. The consumption of considerable amount of glucose was observed when cultured under the aerobic dark conditions than the anaerobic illuminated conditions.