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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 13, Issue 4 - Dec 1985
Volume 13, Issue 3 - Sep 1985
Volume 13, Issue 2 - Jun 1985
Selecting the target year
Properties of Amylase produced from Higher Fungi Ganoderma lucidum
Do, Jae-Ho ; Kim, Sang-Dal ;
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 173~178
Extracellular amylase from tile filtrate of the submerged culture of Ganoderma lucidum was partially purified by ammonium sulfate precipitation and its properties were studied. The optimum pH and temperature of the enzyme activity were 5.5 and 5
. respectively. This enzyme was most stable at pH 5.0 and stable up to 3
, but it lost completely the activity when it was treated at 6
for 10 min. The enzyme was activated by the addition of M
, but inhibited by H
And various enzyme inhibitors and chemical reagents did not affect the enzyme activity. The enzyme hydrolyzed the boiled amylaceous polysaccharides, but it hydrolyzed raw starches very slowly. The activation energy of the enzyme for soluble starch was calculated and found to be 7.06 Kcal per mole. The Km values of the enzyme for soluble starch, amylose, amylopectin and glycogen were 0.16, 0.37, 0.19, and 0.16mg/
, respectively. Maltose was found to inhibit the enzyme activity and kinetic analysis revealed a competitive type of inhibition.n.n.n.n.n.
Citrate Production by Sexually Compatible Strains of Saccharomycopsis lipolytica
Gum, Cho-Seok ; Masayoshi Matsuoka ; Shuichi Aiba ;
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 179~184
Sexually compatible heterothallic haploids and diploids therefrom of Saccharomycopsis lipolytica were compared with respect to production. Diploids constructed through mating were confirmed by random spore analysis, whereas those constructed through protoplast fusion were confirmed by haploidization. ATCC 44601 and IFO 1209 produced larger amount of citrate and isocitrate than IFO 1550 and IFO 1551. A mode of citrate production by diploids was intermediate of the parental haploid strains. The specific activities of citrate synthase and isocitrate lyase in IFO 1550 and IFO 1551 were higher than those in ATCC 44601 and IFO 1209, indicating little correlation between citrate production and specific activities of these enzymes.
Studies on Production of
-Galactosidase by Lactobacillus sporogenes - Purification of Extracellular
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 185~189
-galactosidase from the culture broth of L. sporogenes was purified to apparent homogeniety by procedures including ammonium sulfate fractionation, Sephadex G-200 gel filtration, DEAE-Sephadex A-50 ion exchange chromatography, and Hydroxyapatite adsorption chromatography. The purifying procedures resulted in 347-fold purification with the overall yield of 39.5％ The purified enzyme had a specific activity(using ONPG as a substrate) of about 1, 585 units per mg protein. The molecular weight of the enzyme protein was estimated to be 140, 000 by gel filtration on Sephadex G-200, and SDS-polyacrylamide gel electorphoresis showed that the enzyme consisted of two identical subunits with a molecular weight of 72, 000.
Lactic Acid Fermentation of Lupinseed Milk
Ouk Han ; Tae, Won-Taik ; Kim, Young-Wook ; Lee, Joon-Kyoung ; Lee, Cherl-Ho ;
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 191~198
Seven different strains of lactic bacteria and 13 combinations of these microorganisms were tested for their acid forming capacity on a vegetable milk made from lupinseed protein concentrate(LPC). L acidophilus, L casei, S. lactis, L. mesenteroides, mixed culture of L. acidophilus and S. thermophilus, and mixed culture of S. lactis and L. mesenteroides were selected and further tested for their growth pattern and acid forming property on lupinseed milk both untreated and partly hydrolized one with carbohydrate decomposing enzymes. The enzyme hydrolized lupinseed milk had 1.5 folds of total free sugar, 8.2 folds of fructose, 3 folds glucose, 2.3 folds maltose, compared to the untreated lupinseed milk. For the untreated lupinseed milk, L. mesenteroides was appeared to be most suitable microorganism having the maximum cell concentration of 1.0
and the final pH 4.40 with the acidity 0.46％. For the enzyme treated lupinseed milk, mixed culture of L. acidophilus and S. thermophilus showed the best performance having 1.9
maximum cell number and the final pH and acidity were 3.69 and 1.13％, respectively. Lactic acid fermentation altered the physical property of lupinseed milk; by fermentation the viscosity generally increased with untreated lupinseed milk, but decreased with enzyme hydrolized one. The viscosity change and sedimentation rate of fermented milk varied with the type of lactic bacteria. The results of sensory evaluation indicated that S. lactis, L. casei, mixed culture of S. lactis and L. mesenteroides, and mixed culture of L. acidophilus and S. thermophilus, grown on enzyme hydrolized lupinseed milk, could produce acceptable lactic beverage.
Stability of Plasmid DNA during Liposome Encapsulation
Ahn, J.S. ; Pack, M.Y. ;
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 199~201
Plasmids, YEp13 and pMA56, were encapsulated Into liposomes by two different procedures during which the plasmid DNAs were exposed ether to 6
for 1.5 hr or to sonication for 2-5 min at 4
. The encapsulated plasmids were then reextracted and their physical conformations and transformation abilities were examined. It was confirmed from the results that both plasmid DNAs were remained stable throughout the procedures of encapsulation into 1iposomes.
Effect of Bifidobacterium longum on Growth Inhibition of Enterotoxigenic Escherichia coli
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 203~207
Bifidobacteria are normal inhabitants of the intestinal tract of humans. Using Bif. longum isolated from feces of Korean adult and Bifidus preparation, we observed the growth inhibitory actions of these organisms toward E. coli
causing bacterial diarrhea. Bif. longum SKD-2001 SKD-2004 inhibited the growth of E. coli
drastically. It is supposed that the mechanism of the growth inhibitory actions is due to acid conditions created by Bif. longum.
High Density Cell Cultivation of Escherichia coli in a Dual Hollow Fiber Bioreactor
Chung, Bong-Hyun ; Chang, Ho-Nam ; Kim, In-Ho ;
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 209~212
The cell density and packing characteristics of Escherichia coli immobilized in a dual hollow fiber bioreactor consisting of outer silicone membrane for oxygen transport and three inner isotropic polypropylene hollow fibers for substrate transport were investigated. The cells have grown forming the layer like animal tissue in a nearly 100％ packing density. The dry biomass density was 550g/liter of void volume for cell growth, which was the highest among the biomass densities ever reported.
The Effect of Korean Ginseng Components on the Alcohol Fermentation by Zymomonas mobilis
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 213~221
The effects of ginseng extract, crude saponin and ether layer fraction on the alcohol fermentation and the growth of Zymomonas mobilis studied. The growth of Zymomonas mobilis was inhibited by the addition of ginseng extract, crude saponin and ether layer fraction, of which the last was shown to have the most inhibitory effect. The pH cultural broth recorded a significant decrease during 36 hrs alcohol fermentation, followed by a slower decrease in pH after 36 hrs. The alcohol fermentation was stimulated most remarkably by the addition of 0.305％ crude saponin, while inhibited most by the addition of 0.228％ ether layer fraction.
Screening and Classification of Actinomycetes Producing
-Amylase Inhibitors and the Isolation, their Kinetic Studies of
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 223~232
To find microorganisms of producing
-amylase inhibitors, actinomycetes were isolated from soil samples that were collected at different locations in Korea and screened for enzyme inhibitory activity. A strain of these microbes had a high inhibitory activity and was identified as one of the genus Streptomyces by morphological, biochemical and physiological studies according to the methods of the International Streptomyces Project (ISP). The medium used consisted of 3 ％ corn starch, 0.2％ yeast extract and 0.8％ peptone (pH 7.0). When this strain was aerobically cultured in the medium on a rotary shaker, the highest inhibitory activity was obtained after four days. This inhibitor had inhibitory activities on various
-amylases and glucoamylase, but not on
Isolation of Ethanol-, Acetic acid- and Acetaldehyde- assimilating yeast, Candida sp. JY-5
Jun, Hong-Ki ; Yeehn Yeeh ;
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 233~237
An yeast assimilating ethanol, acetic acid and acetaldehyde among
-compounds was isolated. The morphological, cultural, physiological and biochemical properties of the isolate were examined. As the results of taxonomical researches, the isolate was identified as the genus Candida.
The Growth Characteristics of Candida sp. JY-Cells on Ethanol, Acetic acid and Acetaldehyde Substrate
Yeehn Yeeh ; Jun, Hong-Ki ;
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 239~243
The growth characteristics of Candida sp. JY-5 were examined on ethanol, acetic acid, or acetaldehyde as sole source of carbon by batch culture. The specific growth rates (
) of this strain on an ethanol during tile exponential period were changed depending upon the initial concentrations at above 0.5 g/l, but not in the proportion. The highest
value was 0.291 hr
and the maximum growth yield was 61.2％ at the concentration of 10 g/l. The
values on an acetic acid substrate were constant regardless of the initial concentrations presenting 0.106 hr
in the highest value. The maximum growth yield was shown as 46.8％ at the initial concentration of 10 g/l. The
value on an acetaldehyde during the exponential period was 0.063 hr
and the maximum growth yield was 44.9％ at the initial acetaldehyde concentration of 0.2 g/l.
Citric acid Fermentation by Mutant Strain of Candida lipolytica
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 245~250
In order to increase citric acid productivity. several attempts were made; isolation and characterization of the mutant strain produced citric acid in a high yield, citric acid fermentation in a medium containing relatively higher amount of glucose and citric acid production by the use of semicontinuous ceil recycle system. By the treatment of Candide lipolytica S-109 with NTG, a mutant J-24 was selected as the highest producer of citric acid among the strains formed larger CaCO
lytic zone. it produced 72g/1 citric acid in 10％ glucose medium. Because mutant J-24 produced 85g/l citric acid and showed 53％ yield in 16％ glucose medium, several factors were adjusted to increase the yield in 16％ glucose medium. 0.8-1.0
P/C ratio, 0.15％ urea, 0.25％ yeast extract were suitable at citric acid production in 16％ glucose medium. Under this condition, J-24 strain produced 93g/l citric acid and showed 58％ yield. Semicontinuous cell recycle system was used to protons the effective production phase, to minimize the product inhibition and to shorten the lag phase. The productivity of semicontinuous cell recycle system was 0.79g/l h while that of batch system was 0.53g/l.h
Studies on Nitrogen-Fixing Microorganisms in Rice Rhizosphere
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 251~255
Nitrogen-fixing bacteria were isolated from the rice rhizosphere of various paddy fields in our country. The screening of 235 isolates for nitrogen-fixing ability resulted in the isolation of Enterobacter agglomerans NFB264 and three Klebsiella pneumoniae NFB 3, NFB 320. Plasmids of various molecular weight from 1.7 to more than 84 Mal. were detected by agarose gel electrophoresis in three out of four isolates. But, these plasmids had not any nitrogen-fixing genes. Hybridization experiments using Klebsiella pneumoniae M5al nitrogen-fixing genes, nif Q-K and nif DH, as probes revealed the presence of homologous sequences in the chromosomal DNA of all isolates. However the restriction patterns of nif genes of the isolates by various restriction endonucleases were different to those of Klebsiella pneumoniae M5al.
Evolution of Molecular Hydrogen from Glucose by Rhodopseudomonas sp. KCTC 1437
Woo, Seung-Jin ; Lee, Jeong-Kug ; Kwon, Tae-Jong ; Kho, Yung-Hee ;
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 257~263
Rhodopseudomonas sp. KCTC 1437 evolved molecular hydrogen efficiently under light illuminated anaerobic culture condition in the presence of organic acids and various sugars. especially glucose when low concentration of NH
+ or L-glutamate was added to cultures. It was revealed that hydrogen formation from Rhodopseudomonas sp. KCTC 1437 was mediated by two different enzyme systems. Under the nitrogen limiting condition, hydrogen evolution from glucose was catalyzed by nitrogenase. For the nitrogenase activation in vivo, the precultured cells drown on limiting concentration of NH
as a sole nitrogen source showed more capacity of hydrogen evolution from glucose in the presence of L-glutamate than any other cells .frown on sufficient concentration of NH
, L-glutamate, NH
, or both of L-glutamate and
. A significant volume of molecular hydrogen was evolved from glucose even in the presence of excess NH
either in the light or dark anaerobic condition, presumably due to the mediation of hydrogen evolution by fromic hydrogenlyase.enlyase.
Classification of Bacteriophage of Lactobacillus Casei Strain S-1
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 265~271
The classification of bacteriophage could be followed by several criteria. In this study three criteria were used for classification of Lactobacillus casei bacteriophag. In serological classification. antiserum was prepared by rabbit and used for classification. The inactivation effect of phage by antiserum was exponential and L. casei phage was classified in to three serological groups by inactivation rate (K-values). The Lac Y group was proved as a new serological group but the Lac J and Lac S group were shown the same results as previous reports. From the comparison of restriction enzyme pattern of phage DNA, Lac J group was divided into four sub-groups. According to the difference of host range, Lac J-II group was further subdivided into three groups. These results were shown that L. casei strains S-1 bacteriophage was classified into 8 sub-groups. The phage YK of Lac Y group was shown to consist of a icosahedral head about 95nm in diameter, a contractile tail about 150nm in length and 20nm in width. The tail of YK phage is composed of stacked disks(4nm repeat)and a hexagonal baseplate. The molecular weight of YK phage DNA was approximately 85.6 Mdalton.
Penicillin Fermentation using a Carrier-supported Mycelial Growth
Park, Sang K. ; Kim, Jung H. ; Park, Young H. ;
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 273~278
A carrier-supported mycelial growth of Penicillium chrysogenum was applied to penicillin fermentation system. Among various materials tested, celite was found to be most effective for both spore adsorption and bioparticle development. Hyphal growth through pore matrices of the material showed strong anchorages and provided highly stable biofilm growths. When 5-10％ celite was employed, both cell growth and penicillin production were observed to increase significantly comparing to the dispersed filamentous growth. Specific productivity of penicillin, however. was found to be kept almost constant at a value of 1,900 unit/g cell/hr. A semicontinuous fermentation in a fluidized-bed reactor. using the tarrier-supported biofilm growth, was conducted successfully although free mycelia appeared in the late phase of the fermentation made the reactor operation difficult. Control of the size of bioparticles was considered as a major operating factor to maintain the reactor productivity at a desired level.
Development of L-Lysine Producing Strains by Intergeneric Protoplast Fusion of Brevibacterium flavum and Corynebacterium glutamicum
Kyung, Ki-Cheon ; Lim, Bun-Sam ; Lee, Se-Yong ; Chun, Moon-Jin ;
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 279~283
As a method of breeding L-lysine producing strains, the intergeneric protoplast fusion between Brevibacterium flavum and Corynebacterium glutamicum was performed. As a results, Brevibacterium flavum ATCC 21528 R showed 99％ of protoplast formation and 10％ of regeneration frequencies when treated with 400
of lysozyme for 12hrs. In Corynebacterium glutamicum ATCC 21514 S, 99％ and 12％ were obtained by treatment of 300
lysozyme for 12 hrs. In intergeneric protoplast fusion between Brevibacterium flavum ATCC 21528 R and Corynebacterium glutamicum ATCC 21831 S, 1.0
of recombinant frequency per regenerable cells was observed by use of PEG 6000, 30％(w/v). Among the strains obtained KR
strain showed 12％ higher productivity of L-lysine than the parental cell. Then, the activity of aspartokinase of KR
was about 13％ higher than the parental cell.
Effective Components on the Taste of Ordinary Korean Soy Sauce
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 285~287
To investigate effective constituents of the many taste components in ordinary Korean soy sauce, we analyzed free amino acids, organic acids, free sugars and saline as taste components in ordinary Korean soy sauce, and determined sensory score of the ordinary Korean soy sauce taste with 45 persons of the trained pannels. The relationships between original data transformed with variables and sensory score of the ordinary Korean soy sauce were analyzed by stepwise multiple regression analysis. Eighty five percents of the ordinary Korean soy sauce taste is affected by twenty one kinds (Isoleucine, Leucine, Valine, NaCl, Lactic acid, Alanine, Phenylalanine, Tartaric acid, Sugar(\ulcorner), Proline, Malic acid, Glycine, Tryptophan, Arginine, Glutaric acid, Maltose, Histidine, Glucose, Fructose and Serine) of the taste components by stepwise multiple regression analysis of original data. Eighty one percents of the ordinary Korean soy sance taste is affected by sixteen kinds (Lactic acid, NaCl, Fumaric.Succinic acid, Tyrosine, Tartaric acid, Glycine, Malonic acid, Malic acid, Tryptophan, Glutaric acid, Methionine, Histidine, Cysteine, Maltose, Fructose and (Glutamic acid) of the taste components by stepwise multiple frgression analysis of original data transformed with square root. Eighty five percents of the ordinary Korean soy sauce taste is affected by nineteen kinds (Fumaric.Succinic acid, Lactic acid, Phenylalanine, NaCl, Tyrosine, Sugar(\ulcorner), Tartaric acid, Leucine, Glutaric acid, Methionine, Glycine, Tryptophan, Histidine, Proline, Cysteine, Glutamic acid, Maltose, Threonine and Oxalic acid) of the taste components by stepwise multiple regression analysis of original data transformed with logarithm.
Rapid and Simple Method for Isolating Plasmid DNA from Lactic acid Bacteria
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 289~296
A simple procedure for rapid isolation of plasmid DNA from lactobacillus species and streptococcus species is described. Lactic acid bacteria were cultured in the TCM broth containing 0.5％ glycine and plasmid DNA was isolated from cells treated with mutanolysin by alkaline-detergent lysis method. Good results for releasing and isolating plasmid DNA from lactobacillus species were obtained by treatment of cells with 30
of mutanolysin per ml at 37
for 5 to 10 min. For the streptococcus species, the optimum conditions were slightly different. The procedure could be used for rapid characterization of plasmid DNA in Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus helveticus, Streptococcus lactis, Streptococcus faecalis, Streptococcus faecium, and Streptococcus cremoris strains. Using this procedure, plasmids isolated from
cultures could readily be visualized in agarose gel.
Molecular Cloning and Expression of Bacillus pasteurii Urease Gene in Escherichia coli
Kim, Sang-Dal ; John Spizizen ;
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 297~302
The 7.1 Mdal Xbaf fragment of Bacillus pasteurii ATCC 11859 containing gene for urease was inserted into the Xbal site of bifunctional plasmid pGR71, and its urease gene was cloned and expressed in E. coil RRI. But the cloned gene was not expressed in Bacillus subtilis BR151 in consequence of deletion of inserted DNA fragment. The recombinant plasmid thus formed was named pGU66. The restriction map of the plasmid pGU66 was determined, and the size of the plasmid was estimated to be 12.6 Mdal by double digestion of restriction enzymes of the plasmid. The urease of the cloned strain was accumulated in periplasmic space and very similiar to that of donor strains in their enzymatic properties.
Biofuel Production by Immobilized Living Cells - Hydrogen Production by Photosynthetic Bacteria -
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 303~309
Continuous production of hydrogen by Ca alginate-immobilized photosynthetic bacteria was studied in a packed-bed bioreactor. The dilution rate and input concentration of carbonaces substrate were selected as operating parameters. To choose the strain for immobilization, hydrogen productivities of Rhodopseudomonas caposulata 10006 and Rhodospirillum rubrum KS-301 were compared through preliminary batch cultures of their free cells: the former was found to show better hydrogen productivity in spite of its lower specific growth rate. For the continuous production of hydrogen by immobilized R capsulata, the optimum dilution rate was about 0.84 h
. The Immobilized tells gave better hydrogen yield and conversion efficiency than free ones. And a kinetic parameter K'
was determined for the packed-bed bioreactor, being practically constant for a specific range of dilution rates.s.
Protoplast Formation and Regeneration of Ganoderma lucidum
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 311~314
Ganodema lucidum protoplasts were formed by the treatment of Novozym 234. The osmotic stabilizers such as mannitol were effective enough to produce protoplasts up to 10
. For regeneration, however, MgSO
was suitable. When inositol and sucrose were employed as osmotic stablizers, the regeneration ratio reached to 0.26％. Overlay of Streptomycin sulfate added agar was required to prevent bacterial contamination.
E. M. Visualization and Electrophoresis analysis of B. thuringiensis var. kurstaki and B. thuringiensis var. israelensis
Microbiology and Biotechnology Letters, volume 13, issue 3, 1985, Pages 315~319
Delta-endotoxin crystals of B. thuringiensis var. kurstari and B. thuringiensis var. israelensis were purified by NaBr density gradient centrifigation and the wet weight of the BTK endotoxin was approximately 23.79％ of the cell wet weight and that of BTI was 25％. The shape of BTK crystal was bipyramidal, whose size was 1.7
and that of BTI was a spheroid, whose size was about 1.6
. The molecular weight of BTK crystal protein was approximately 134,000 daltons and that of BTI was about 128,000 daltons.