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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 13, Issue 4 - Dec 1985
Volume 13, Issue 3 - Sep 1985
Volume 13, Issue 2 - Jun 1985
Selecting the target year
Studies on the production and purification of an extracellular protease from a nonpigmenting Serration sp.
Kim, Soung-Soo ;
Microbiology and Biotechnology Letters, volume 13, issue 4, 1985, Pages 321~327
Cultivation conditions for the production of extracellular alkaline protease by a nonpiamentation Serratia sp. and purification of the enzyme were studied. The maximum enzyme level was obtained at the beginning of stationary phase when the organism was cultured on brain heart infusion medium at
under aeration (gyratory shaking, 180 cycles/min). The enzyme was purified about 100 fold with 16.5％ yield by ammonium sulfate precipitation, ammonium sulfate fractionation followed by DEAE-cellulose chromatography (1st and 2nd). The purified enzyme moved as a single symmetrical peak in the analytical ultracentrifuge. The enzyme demonstrated its maximum activity at pH 8.5-9.0 and 4
when vitamin-free casein was used as a substrate.
Studies on Digestion of Raw Starch by Rhizopus oryzae - Optimum Condition of Enzyme Production and Ethanol Fermentation -
Microbiology and Biotechnology Letters, volume 13, issue 4, 1985, Pages 329~337
A potent mold strain was selected to digest raw starch, which was classified as a strain of Rhizopus of zoe. Its amylase production was maximized when grown on wheatbran media for 3 days at 3
and initial pH 4. The crude enzyme was tested for ethanol fermentation by yeast, Saccharomyces cerevisiae IFO 7026, on various starchymaterials and the ethanol production after 4 days was: 9.4％ from rice powder, 9％ from corn powder, 8.1％ from sweet potato powder, and 5.4％ from potato powder, respectively.
Inhibitory Effect of Cephalosporin C on Growth of Cephalosporium acremonium M-113
Kim, Myung-Kuk ; Park, Sang-Ho ; Lee, Jeong-Kug ; Kho, Yung-Hee ; Mheen, Tae-Ick ;
Microbiology and Biotechnology Letters, volume 13, issue 4, 1985, Pages 339~344
Cephalosporin C(CPC) inhibited the growth of Cephalosporium acremonium M-113, a potent CPC producer derived from C acremonium ATCC 20339. Similar inhibitory effects of CPC were also observed in growth of C. acremonium ATCC 20339 and ATCC 14553. Minimum inhibitory concentrations (MIC) of CPC on the growth of conidia and hyphae of C. acremonium M-113 were 200-500 and 3000-4000
respectively in synthetic medium. MIC values were increased in complex media. The inhibitory effect of CPC was due to CPC-exerted inhibition of amino acids uptake by the cells. 3'-Group of CPC might be important in its inhibitory action. In audition, CPC itself could be utilized by the cells as a nitrogen source under nitrogen limited condition.
Transformation of Bacillus subtilis Protoplast by Recombinant Plasmid DNA
Kim, Sang-Dal ; John Spizizen ;
Microbiology and Biotechnology Letters, volume 13, issue 4, 1985, Pages 345~348
Recombinant chimeric plasmid constructed with Xba I digested pUBl10 and -pE194 was transformed by polyethylene glycol induced protoplast transformation system into Bacillus subtilis BR 151 on the mannitol regeneration media, and two genes of antibiotics resistance were expressed simultaneously in the transfromant. Transformation frequency of the recombinant plasmid was 6.5
on the mannitol regeneration agar plate containing neomycin and erythromycin. The replication of recombinant plasmid in the recipient cells was confirmed by the alkaline extraction method and agarose gel electrophoresis.
Molecular Cloning of a Thermostable
-Amylase Gene from Bacillus stearothermophilus and Its Expressions in E. coli
Huh, Tae-Lin ; Koh, Suk-Hoon ; Lee, Se-Yong ;
Microbiology and Biotechnology Letters, volume 13, issue 4, 1985, Pages 349~354
A 4.7 kb Hind III fragment containing
-amylase gene of Bacillus stearothermophilus IAM 11062 was cloned in Escherichia coil HB101, using plasmid pBR322 and runaway plasmid pSY343 as a vector. The cloned gene was stably maintained and expressed In E.coli. The constructed strain of E. coli have at least 3 times higher amylase activity than the donor strain, of B. stearothermophilus. About 75％ of the
-amylase produced by the constructed strain of E. coli was localized in the periplasm and it was found that the enzymes can be released by an osmotic shock using EDTA. The enzymatic properties of L-amylase produced in E. coli were very similar to those produced by B. stearothermophilus in terms of optimum temperature, heat stability and molecular weight
Studies on the Production of
-Galactosidase by Lactobacillus sporogenes - Properties and Application of
Microbiology and Biotechnology Letters, volume 13, issue 4, 1985, Pages 355~359
-galactosidase from L. sporogenes was most active at pH 7.0 and 6
-D-galactopyranoside (ONPG) in 0.05 M phosphate buffer. It was stable over a pH range from 5.0 to 9.0 and lost less than 10％ of its activity after heating for 30 minutes at 6
and pH 7.0. All the mineral ions examined in this work showed no significant activating effect, whereas L-cysteine exerted a great stimnlatory effect on the enzyme activity at the concentration of 10 mM. The Km values were 1.2 mM for ONPG and 33.3 mM for lactose. Approximately 85％ of lactose in cow's milk, in 10％ skim milk and in 5％ lactose solution was hydrolyzed after 4 hours incubation at 6
with 2 units of the purified
of the substrate solutions. The
-galactosidase from L. sporogenes, therefore, is considered to be suitable for hydrolysis of lactose in milk and other dairy products.
Distribution and Substrate Specificity of 5-fluorocytosine Deamiase in Bacteria
Microbiology and Biotechnology Letters, volume 13, issue 4, 1985, Pages 361~366
Distribution and substrate specificity of 5-fluorocytosine deaminase were studied in various genera of bacteria. 5-Fluorocytosine deaminase was produced by various bacteria independent of genus and species and it catalyzed the deamination of cytosine, 5-fluorocytosine and 5-methylcytosine. Xanthomonas campestris IAM 1671 produced relatively large amount of 5-fluorocytosine deaminase. The composition of optimum culture medium for enzyme production wat glycerine 0.5％, peptone 1％, yeast extract 0.5％, NaCl 0.5％ and the initial pH of the medium was 7.5. The highest enzyme formation was observed after 24 hours of cultivation In 500
shaking flask containing 90
of medium at 3
on a reciprocal shaker.
Isolation and Identification of Spoilage Microorganism from Ginseng Extract
Microbiology and Biotechnology Letters, volume 13, issue 4, 1985, Pages 367~370
To find the bases for the preservation of red ginseng extract, the oamophilic yeast was isolated from the spelled red ginseng extract. The isolated yeast was identified as a strain of Candida parapsilosis.
Growth Characteristics of Candida parapsilosis Isolated from Deteriorated Ginseng Extract
Microbiology and Biotechnology Letters, volume 13, issue 4, 1985, Pages 371~376
We investigated the growth parameters of Candida parapsilosis isolated from spoiled red ginseng extract. The optimum pH range for C. parapsilosis was 6.0, whereas the minimum and maximum pH values that permitted growth were 3.0 and 11.0, respectively. For cells grown in PG medium plus 0 and 60％ sucrose, the optimum water activity(Aw) values were 0.98 and 0.97, respectively. The optimum temperature for C. parapsilosis were 3
at an Aw of 0.90 in 4.8％ potato dextrose broth with 18％ sucrose (PGS). Cations inhibiting the growth of C parapsilosis were L
in decreasing order, while anions were S
Factors Affecting Protoplast Formation of Yeast
Kim, Young-Ho ; Seu, Jung-Hwn ;
Microbiology and Biotechnology Letters, volume 13, issue 4, 1985, Pages 377~382
As an essential previous step towards the development of cell fusion to breed a new brewing yearst strain, several factors predicted to affect the protoplast formation of S. cerevisiae, C. tropicalis and E. fibuligera were investigated in order to obtain the protoplasts in high yields. The optimum pH and temperature for the protoplas formation were 7.5 and 35
, respectively. Pretreatment of the yeast cells with 2-mercaptoethanol stimulated the protoplast formation and 50mM of the reagent was found as effective. Among several osmotic stabilizers tested for their effect on protoplas formation, 0.6M KCI was comparatively favorable.
Conditions for Intergeneric Protoplast Fusion of Yeast
Kim, Young-Ho ; Seu, Jung-Hwn ;
Microbiology and Biotechnology Letters, volume 13, issue 4, 1985, Pages 383~389
Optimum conditions of PEG treatment for the intergeneric fusion of yeast protoplasts were investigated. Fusants were selected by nutritional complementation on minimal medium. The intergeneric fusion frequency between pro-toplasts of S. cerevisiae and C. tropicalis was distributed 10
, depending on the combination of parental strains. PEG 4000 or 6000 are equally effective. 30%(w/v) PEG 4000 was found to be optimum and below 20% its stabilizing effect was lost, resulting in protoplast lysis, and optimum pH was 8.0. The efficiency of PEG was enhanced by higher temperature of the PEG solution, and by the addition of Ca ions. The stimulating effect of Ca ions in the range of 1 mM to 100 mM proved similar.
Intraspecific Protoplast Fusion of Citric Acid Producer, Candida lipolytica
Microbiology and Biotechnology Letters, volume 13, issue 4, 1985, Pages 391~395
In order to develope a protoplast fusion system for citric acid and SCP producing Candida lipolytica, the optimal conditions for the formation and regeneration of protoplast were examined and the protoplast fusion was performed. At the optimal conditions of growth phase and Zymolyase treatment, frequencies of protoplast formation were 98%. Approximately 20-30% of protoplasts were regenerated on the regeneration minimal medium containing 3% agar and 30mM
with the overlay of the same medium. The fusion frequencies, 4-5
, were accomplished by the treatment of two nutritionally complementary auxotrophic protoplasts, L-14 (
) and T-24 (X
), with 30% PEG 6000 containing 100mM
for 20 minutes.
Studies on the Ethanol Production by Clostridium thermosaccharolyticum
Microbiology and Biotechnology Letters, volume 13, issue 4, 1985, Pages 397~402
The fermentation of various sugars by C. thermosaccharolyticum was examined under pH controlled, anaerobic condition. The kinetic model for Product formation at various sugars was the combination of growth and non-growth associated mode. In the utilization of a single sugar, glucose was the best carbon source for growth. The specific growth rate of glucose, xylose and cellobiose were 0.363 h
, 0.242 h
and 0.144 h
respectively. The production of ethanol from glucose showed a negatively growth associated mode, so the higher growth rate decreased the productivity of ethanol. The maximum concentrations of the produced ethanol were 2.42 g/l, 3.76 g/l, and 3.4 g/l on glucose, xylose, and cellobiose. No glucose was detected during cellobiose fermentation. Sequential utilization of sugars was observed in the mixtures of glucose, xylose and cellobiose. It preferred glucose, followed by xylose and then cellobiose. The presence of other sugars had little or no effect on the rate of another sugar utilization. Cell lysis at the end of fermentation occured more slowly in the mixtures of sugars than a single sugar.
Enhancement by Surfactant on Release of
-Amylase and Phosphatase in Submerged Culture of Rhizopus oryzae
Microbiology and Biotechnology Letters, volume 13, issue 4, 1985, Pages 403~408
Enhancement of surfactant on release of secretory enzyme, such as
-amylase, acid phosphatase and alkaline phosphatase, was investigated during submerged culture of Rhizopus oryzae. Morphological changes of colony was occured; small pelletal form in 0.18mM of sodium dodecyl sulfate, pulpy form in 0.48mM of sodium deoxycholate, and filamentous form in absence of surfactant. It. Supplement of sodium dodecyl sulfate induced 9 times increasing activity of
-amylase and that of acid phosphatase 25 times in cultural fluids. Alkaline phosphatase was increased 11 times in cultural fluid and also stimulated in cytoplasm with supplement of sodium deoxycholate.
Bacterial Quality of Fish Meat Paste Products and Isolation of Thermoduric Bacteria
Microbiology and Biotechnology Letters, volume 13, issue 4, 1985, Pages 409~415
This study has been carried out in order to investigate the bacterial quality of fish meat paste products and the characteristics of isolated thermodurics from the products. Twenty samples of crab-flavored fish stick (Kematsal), 23 samples of plate fish meat paste (Panomuk, Kamaboko), 5 samples of fried fish meat paste (Tigimomuk), 2 samples of roasted fish meat paste (Puduromuk, Chikuwa), 20 samples of fish sausage were collected from processing plants and supermarkets in Pusan, Korea during the period from May to October in 1984. The results obtained are as follows. Amont the samples collected from supermarkets, roasted fish meat paste and fried fish meat paste marked hish counts in coliforms and fungi while very low in the samples of crab-flavored fish stick and plate fish meat paste. Salmonella was not detected in all the samples examined and Staphylococcus aureus was detected only in fried fish meat paste, Thermoduric bacteria were detected less than 10
/g in the samples of crab-flavored fish stick and plate fish meat paste, which might come from subsidiary materials such as starch and seasonings. Among the isolated bacteria, distribution of the proteolytics were more than 87％ and the lipolytics were less than 20％. Gram positive bacteria was more than 70％ in crab-flavored fish stick and plate fish meat paste, 47.3％ in fried fish meat paste. And rod in shape was almost more than 90％ in all the samples. The most heat resistant bacterium isolated from the samples was identified as a Bacillus licheniformis(named B. licheniformis CR-11). The strain showed strong proteolytic activity and also grew well at above 2
. The growth rate and generation time of CR-11 strain were 0.31 hr
, 2.24 hr at 2
, 0.64 hr
, 1.09 hr at 3
and 0.78 hr
, 0.89 hr at 35
. Heat resistance value of the spores of CR-11 strain suspended in phosphate buffer solution was D
=41.9 min, D
=27.9 min, D
=10.2 min, D
=4.3 min (Z=13.8
Characterization of Plasmid DNA in Streptococcus faecalis var. liquefaciens
Microbiology and Biotechnology Letters, volume 13, issue 4, 1985, Pages 417~422
Streptococcus faecalis var. liquefaciens was examined for the presence of plasmid deoxyribonucleic acid. An analysis by agarose gel electrophoresis revealed the presence of at least four plasmids of approxymately 6.8, 5.2, 2.6, and 2.1 Mdal. Two plasmid cured strains were obtained by novobiocin treatment. SKR2, which lost 5.2 mdal plasmid (pSK2) and 2.1 Mdal plasmid(pSK4) was sensitive to lincomycin and erythromycin. However, all cured strains showed identical response as parental strain in sugar fermentation, temperature sensitivity, proteolytic activity, and liquefaction of gelatin. The results imply that pSK2 or pSK4 is associated with antibiotic resistance of Str. faecalis var. liquefaciens.