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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 14, Issue 6 - Dec 1986
Volume 14, Issue 5 - Oct 1986
Volume 14, Issue 4 - Aug 1986
Volume 14, Issue 3 - Jun 1986
Volume 14, Issue 2 - Apr 1986
Volume 14, Issue 1 - 00 1986
Selecting the target year
Cloning of Bacillus amyloliquefaciens amylase gene using YEp 13 as a vector II. Expression of cloned amylase gene in Saccharomyces cerevisiae
Microbiology and Biotechnology Letters, volume 14, issue 3, 1986, Pages 209~212
-Amylase gene of Bacillus amyloliquetaciens was cloned on plasmid YEp13, S. cerevisiae-E. coli shuttle vector. Hybrid plasmid pTG17, carrying
-amylase gene of B. amyloliquefaciens, was transformed to E. coli and the expression of it in yeast was investigated. This plasmid was unstable in E. coli and produced two minor plasmids, pTG17-1 and PTG17-2, which resulted from the segregation of it. Transformant of S. cerevisiae MC16 with pTG17-1 plasmid was not appeared on SD medium because of the Leu2 gene defection. S. cerevisiae could be transformed by the hybrid plasmid, and
-amylase activity of the yeast transformant was detected by somogyi-Nelson method and agar diffusion method.
Cloning of Bacillus amyloliquefaciens amylase gene using YRp7 as a vector II. Expression of cloned amylase gene in Saccharomyces cerevisiae
Microbiology and Biotechnology Letters, volume 14, issue 3, 1986, Pages 213~218
Hybrid plasmid pEA24, shuttle vector YRp7 carrying amylase gene of Bacillus amyloliquefaciens, was transformed to yeast Saccharomyces cerevisiae, and the expression of B. amyloliquefaciens amylase gene in yeast was investigated. The frequency of transformation to S. cerevisiae DBY747 with YRp7 was increased by treatment of 40% polyethylene glycol (MW 4, 000), PH 7.0, at 3
, and by regeneration used 2% top agar. The amount of cellular amylase activity produced by S. cerevisiae containing pEA24 was 2% of that secreted from B. amyloliquefaciens, but in case of S. cerevisiae transformant, the amylase secreted was not detected. A comparison of genetic stability of pEA24 and YRp7 plasmids in yeast was carried out by cultivation of transformants in tryptophan-supplement-medium. The pEA24 plasmid was more unstable than YRp7 in S. cerevisiae. The size of pEA24 extracted from S. cerevisiae transformants was found to be identical with that from E. coli transformants by agarose gel electrophoresis.
Preparation and Characterization of Cell Hybrids Producing a Monoclonal Antibody to Human Fibroblast Interferon (Hu IFN-
Microbiology and Biotechnology Letters, volume 14, issue 3, 1986, Pages 219~223
In order to preparr the hybridoma cells which produce a monoclonal antibody to human fibroblast interferon(Hu IFN-
), spleen cells from BALB/cmice immunized with the purified Hu IFN-
were fused with NS-O cells, a myeloma cell line. Forty hybrids with high titer among 1300 hybrids Isolated by an ELISA screening method were subcloned using the soft agarose cloning and limiting dilution methods, and 11 hybrids were selected. As a result of iso-typing the hybrids using the mouse monoclonal typing kit, two hybridomas were found to produce 1gG 2a type of monoclonal an-tibodies. The ascites fluid from nude mice inoculated intraperitoneally with the above hybridomas was removed and purified using a protein A-Sepharose CL-4B. Monoclonal antibody was proven to have only the heavy and light chains on SDS-polyacrylamide gel electrophoresis.
Effect of Garlic on the Quality of Barley Kochuzang Brewed with Whole Red Pepper
Microbiology and Biotechnology Letters, volume 14, issue 3, 1986, Pages 225~232
This study was to improve the quality of Kochuzang and utilize red pepper seed. Kochuzang were prepared with the addition of red pepper seed and garlic (2％), compared the changes in the various chemical components and enzyme activity during the aging period of Kochuzang, and also organoleptic values of the products. Enzyme activities of liquefying and saccharogenic amylase, protease and lipase were increased by addition of garlic pulp and the suvival activities of enzyme except liquefying amylase were lasted high the late period of aging. Also the addition of red pepper seed was effective in maintaining the enzyme activities Change of titration acidity and pH of kochuzang were little when red pepper seed was added, but in case of a garlic additive it showed no difference at the late period of aging. Total nitrogen and amino nitrogen were increased by the addition of red pepper seed or garlic until the late period of aging, and ammonia nitrogen also increased during the middle period of againg, but showed no difference at the late period. Alcohol content was decreased by the addition of garlic or red pepper seed. but crude lipid was increased by the audition of red pepper seed. but crude lipid was increased by the addition of red pepper seed. Generally, taste, flavor and color of garlic added group were superior to the non-garlic added group for the products which aged for 10 weeks. Therefore, The quality of Barley Kochuazng may be improved by adding 2％ garlic to the whole red pepper.
Large Scale of Ethanol Fermentation from Sweet Potato Cooked at Low and High Temperature
Microbiology and Biotechnology Letters, volume 14, issue 3, 1986, Pages 233~237
Possibility of large scale ethanol fermentation from sweet potato were compared with low temperature and high temperature rooking. Productivity of sweet potato mash cooked at 9
for 120 minutes was higher than that mash cooked at 124
for 60 minutes and also fermentation yield ai low temperature cooking was better than high temperature cooking. Low temperature cooking was successfully carried out on a large scale. In conclusion, low temperature cooking on large scale should be reduce energy consumption by approximate 30% compared with high temperature cooking.
Physiological properties and transformation of alkaline-tolerant bacteria
Microbiology and Biotechnology Letters, volume 14, issue 3, 1986, Pages 239~244
To develop the potential use as new host strain for gene cloning, alkaline-tolerant isolates from soil were examined for amylase activity, protease activity, antimicrobial activity and transformability by using plasmid pUB 110. Of these strains, one was selected and identified as Bacillus sp. YA-14. in the enzymatic properties of Bacillus sp. YA-14 the optimal conditions for the reaction of amylase and protease were at pH 0.8 and pH 7.5 respectively. The antimicrobial activity of Bacillus sp. YA-14 was also found. For the transformation, Bacillus sp. YA-14 was cultured to late logarithmic growth phase ai 37
in modified SPI medium (pH 8.0) containing 0.4% MgSO
. The presence of pUB 110 plasmid DNA in transformants was confirmed by electrophoresis and stably maintained in the new host.
Transfer and genetic recombination of antibiotic resistance genes occurring in water environment
Microbiology and Biotechnology Letters, volume 14, issue 3, 1986, Pages 245~250
Antibiotic resistant bacteria isolated from the river water in Cheongju City were studied on the transfer of their R-plasmids in water environment. The R-plasmids were transferred by conjugation between the isolates ai the frequencies of 1.1
under laboratory condition and 1.2
in natural environment. The R-plasmids isolated from those bacteria were also transferred into the recipient cells of E. coli HB101 at the frequencies of 1.7-6.7
. The AP
-plasmid of isolate T-44 which were transformation by conjugation and transformation was determined to be 9.01 kilobses in molecular size. When the AP
-plasmid DNA was treated with restriction endonuclease, the plasmid appeared to have one restriction site for EroRI and BamHI, respectively, and three sites for Pst I endonuclease.
A study on the protoplast formation and regeneration of Lactobacillus casei YIT 9018
Baek, Young-Jin ; Bae, Hyeong-Suk ; Min Yoo ; Kim, Young-Kee ; Kim, Hyun-Uk ;
Microbiology and Biotechnology Letters, volume 14, issue 3, 1986, Pages 251~257
Optimum conditions for the protoplast formation and regeneration of Lactobacillus casei have been searched for. L. casei cells were converted to protoplast by treating with 10
of mutanolysin in 20mM potassium phosphate buffer (pH6.8) containing 6mM CaCl
, 6mM MgCl
and 1M sucrose. Maximum number of protoplasts was obtained when cells were taken from Tomochika's medium containing 0.5% glycine at the middle to late logarithmic growth phase. Regeneration was efficiently accomplished on the regeneration medium containing 6mM CaCl
, 6mM MgCl
, 0.8M sucrose and 10% of horse serum. The efficiently of the cell wall regeneration from protoplasts was 2-5% after 3-4 days of incubation at 3
Studies on the Development of the Bacillus thuringiensis is Pesticide - Conditions of delta- endotoxin production by B. thuringiensis serovar kurstaki-
Microbiology and Biotechnology Letters, volume 14, issue 3, 1986, Pages 259~264
The compositions of the four madia and their pHs for delta endotoxin production by B. thuringiensis serovar kurstaki 3a3b were examined. In the M-4 media out of the 4 media at pH8, the production of the endotoxin and spore formation were maximal. The mean generation times of the bacterium were 53.4 minutes in the M-1 media, 98 in the M-2, 132 in the M-3, and 127.5 in the M-4. The proper pHs of the media for the endotoxin production appeared to be pH 7 to 8. In the M-4 media, the lag time lasted about 5 hours.
Studies on the Protoplast Fusion of Lactobacillus casei
Baek, Young-Jin ; Min Yoo ; Kim, Young-Kee ; Bae, Hyeong-Suk ; Kim, Hyun-Uk ;
Microbiology and Biotechnology Letters, volume 14, issue 3, 1986, Pages 265~270
The best conditions for the protoplast fusion of Lactobacillus casei have been searched for in this study. Antibiotic resistance was used as the selective marker for enumerating and selecting the recombinants. Antibiotic resistant mutants were isolated after treating cells with N-methyl-N'-nitro-N'-nitrosoguanidine. High frequency fusion of protoplasts of L. casei strains were obtained in the presence of 40% (wt/vol) polyethylene glycol 4,000 after 1 min at 3
at around neutral pH. Spontaneous mutations of drug-resistance of L. casei were two or three orders lower than the recombination frequency. Recombination frequencies were about 10
per parent cells employed.