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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 14, Issue 6 - Dec 1986
Volume 14, Issue 5 - Oct 1986
Volume 14, Issue 4 - Aug 1986
Volume 14, Issue 3 - Jun 1986
Volume 14, Issue 2 - Apr 1986
Volume 14, Issue 1 - 00 1986
Selecting the target year
Mutagenesis of Sisomicin-producing Strains and Selection Method of High Producers
Microbiology and Biotechnology Letters, volume 14, issue 4, 1986, Pages 271~277
A sisomicin-producing strain, Micromonospora inyoensis, was treated with various mutagens. The optimal death rates to obtain the high-producers of sisomicin were 90% for ultraviolet light, 99% for nitrosoguanidine, and 99.3% for nitrous acid, respectively. In place of the method of liquid culture, an agar plug method, which is easy and convenient, was used to measure the antibiotic-producing abilities of the strains isolated from mutagen-treated cells. On the other hand, as the selection method of overproducing mutants after mutagenesis, gradient agar plates which included various antibiotics and salts were used. Among the antibiotics and salts tested, gentamicin and kanamycin as antibiotics, and CuCl
as salts, were effective to select the high-producers of sisomicin.
Effect of ginseng residue on the growth of Ganoderma lucidum
Kim, Sang-Dal ; Do, Jae-Ho ; Lee, Kwang-Seung ;
Microbiology and Biotechnology Letters, volume 14, issue 4, 1986, Pages 279~283
The ginseng residue, a by-product of ginseng tea manufacture, was used as growth promoting substance in the submerged cultivation of Basidiomycetes for its effective utilization. Ginseng residue contained about 46％ of total sugar, 14％ of crude protein, 12％ of ash, and 0.16％ of crude saponin. Among inorganic substances in ginseng residue, amount of Mg, Na, K and Ca were much more than other inorganic substances. All ginsenosides existed in red ginseng residue. Ganoderma lucidum and Pleurotus ostreatus could be excellently cultured in potato dextrose broth. Most effective additional concentration of residue extract for growth of these fungi was shown to be 0.2％, and the contents of crude protein and amino acid in mycelium were increased when 0.5％ of residue extract were added to the medium.
Citric Acid Production Using Immobilized Yeast Activated with
- containing Medium
Microbiology and Biotechnology Letters, volume 14, issue 4, 1986, Pages 285~291
Immobilized Candida lipolytica cells were prepared by entrapping the whole cells in calcium alginate gel. To enhance citric acid productivity, immobilized cells were Incubated with activation medium in fluidized-bed reactors. When the activation was done in batch operation, maximum citric acid productivity appeared in a much shorter time than in continuous operation. Activated immobilized cells were enhanced about 10-fold in citric acid production relative to non-activated immobilized cells. The productivity of citric acid was also influenced by bead size. When Immobilized cells were reacted in a fluidized-bed reactor with the same quantity of cells, the citric acid productivity was increased as the bead size was decreased
Effects of Various Carbon Sources and Carbon Catabolite Regulation in Sisomicin Fermentation
Microbiology and Biotechnology Letters, volume 14, issue 4, 1986, Pages 293~298
Sisomicin, which is one of aminoglycoside antibiotics, was produced by Micromonospora inyoensis. The effects of carbon sources on sisomicin production were studied in batch cultures. Starch, dextrin and maltose were good carbon sources for the production of sisomicin. However, when glucose was used, the antibiotic productivity decreased significantly due to a carbon catabolite regulation. The carbon catabolite regulation depends mostly on carbon catabolite repression, but not on carbon catabolite inhibition. On the other hand, the growth-production curves of batch cultures show that sisomicin is produced most actively during the idiophase.
Studies on the Ploidy of Saccharomyces cerevisiae
Microbiology and Biotechnology Letters, volume 14, issue 4, 1986, Pages 299~304
The cell volume, cell surface, cell concentration, dry cell weight, frequence of respiratory deficient mutation, resistance against ultraviolet irradiation, fermentation power, DNA contents of haploid diploid, triploid and tetraploid of Saccharomyces cerevisiae strain were investigated. Respiratory deficient mutants by spontaneous mutation were absolved more frequently in the haploid than in the diploid, triploid and tetraploid. And cell volume, cell surface, cell concentration, dry cell weight, resistance against ultraviolet irradiation, fermentation power, and DNA contents were significantly increased as the ploidy increased.
A study on strain improvement by protoplast fusion between amylase secreting yeast and alcohol fermenting yeast I. Isolation and characterization of fusant between S. cecevisiae and S. diastaticus
Microbiology and Biotechnology Letters, volume 14, issue 4, 1986, Pages 305~310
To improve the starch fermentation ability of yeast, hybrids were introduced by protoplast fusion of S. cerevisiae and S. diastaticus. The protoplasts of parental auxotrophic cells were fused in the presence of 10 mM CaCl
and 30% of polyethyleneglycol (M.W 4, 000). The frequencies of fusant formation varied depending upon the strains used and were 3.51
for the regenerated protoplasts. The strains capable of extensive starch hydrolysis produce only 10% to total fusants. The 4 strains were finally selected by the results of starch fermentation and genetic stability test. The DNA content and cell volume of the fusants were greater than those of the parental strains.
A study on strain improvement by protoplast fusion between amylase secreting yeast and alcohol fermenting yeast - ？
. Alcohol and glucoamylase productivities of fusant between S. cerevisiae and S. diastaticus
Microbiology and Biotechnology Letters, volume 14, issue 4, 1986, Pages 311~318
Glucoamylase and ethanol productivities of HSDD-170 and HSDM-119 formed by S. cerevisiae and S. diastaticus protoplast fusion were investigated. For the production of the glucoamylase, soluble starch as carbon source, yeast extract and C. S. L as nitrogen source added into the basal medium were favorable. The production of the enzyme reached at maximum after cultivation of the fusant for 4 days at 3
, aerobically. The properties of glucoamylase produced by fusants were very similar to those produced by S. diastaticus as based on optimum temperature, pH stability. In alcohol fermentation from starch, strain HSDD-170 fermented starch faster than either of its parental strains. The maximum of alcohol yield in 15% of liquefied potato starch was 7.5% (v/v).
Studies on the Genetic Recombination by Intraspecific Fusion of Lactobacillus casei Protoplast
Young Jin Baek ; Hyeong Suk Bae ; Young Kee Kim ; Min Yoo ; Hyun Uk Kim ;
Microbiology and Biotechnology Letters, volume 14, issue 4, 1986, Pages 319~324
After intraspecific fusion of Lactobacillus casei protoplasts, the recombinants have been studied for their lactose utilization, protease activity and phage resistance. L. casei C-M phenotypes constituted 46% of the fused cells when tested against phages, and L. casei 3-M phenotypes 42% of the fused cells, and 12% of the recombinants developed the resistance to both parent types of phages. The acid production and proteolytic activity of recombinants evidenced the similar trends. There was no difference in Hind III digests of plasmid DNA between parent cells and recombinants, but the reconlbinant cells were found to possess only one type of plasmid, either of 1. casei C-M or of L. casei 3-M.
Studies on the Development of the Bacillus thuringiensis - Lethality and safety tests of the endotoxin and the pesticide of B. thuringiensis serovar kurstaki -
Microbiology and Biotechnology Letters, volume 14, issue 4, 1986, Pages 325~328
The lethality to Pieris raepi larvae and the safety tests to mice of the endotoxin crystals and the pesticide of B thuringiensis serovar kurstaki were carried out. The LD50 of the endotoxin (3.9
) appeared to be 1
g and that of the pesticide (2.6
) was about 9
g. When the endotoxin solution and the pesticide were injected to the mice's intraperitoneal cavities, 10 to 20% of the mite were dead. Also in the case of intracerebral injection 20% of the mice were dead at the doses of 7.8
spores and 100% of the mice were dead at the concentration of 7.8
spores. but the mice in the oral, subcutaneous and inhalation treatment groups were safe and healthy. When the pesticide applied to the cabbage field for raepi larvae, 52 to 65% of the larvae in the field were killed in 4 days postapplication.
Studies on the Development of the Bacillus thuringiensis Pesticide - Media compositions for the endotoxin production by B. thuringiensis var israelensis -
Microbiology and Biotechnology Letters, volume 14, issue 4, 1986, Pages 329~334
Bacillus thuringiensis serovar israelensis H 14 strain was cultured in 4 different fermentation M-media and then measured the rates of their growths and the productions of endotoxin crystals front the media. Out of the four M-media the production of endotoxin crystals and spores was maximal in M-4 medium (pH 9). The wet weight of the cells grown in the 150
culture was approximately 3.901g and the number of viable spores was 1.53
per nil and the ratio of the endotoxin over the total cell weight was 18.54%. The generation time was about 89.3 minutes in the M-1 medium, 124.1 minutes in the M-2, 97 minutes in the M-3, 130.8 minutes in the M-4. The proper pHs for the production of the endotoxin appeared to be 6.5 to 7.5.