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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 14, Issue 6 - Dec 1986
Volume 14, Issue 5 - Oct 1986
Volume 14, Issue 4 - Aug 1986
Volume 14, Issue 3 - Jun 1986
Volume 14, Issue 2 - Apr 1986
Volume 14, Issue 1 - 00 1986
Selecting the target year
Factors affecting sisomicin production by Micromonospora inyoensis
Lee, Jae-Heung ; Gil, Gwang-Hoon ; Cho, Young-Je ; Yoo, Moo-Young ;
Microbiology and Biotechnology Letters, volume 14, issue 5, 1986, Pages 355~358
The effects of cobalt chloride, methionine, and various carbon sources on the sisomicin production by M. inyoensis NRRL 3292 were investigated. It was found that both cobalt chloride and methionine exerted a greater stimulatory effect on sisomicin formation. Kinetic studies with various carbon sources revealed thai polysaccharide such as starch or dextrin was found io be better than glucose for sisomicin production Moreover, the relatively low concentration of dissolved carbon dioxide was one of the most important factors In accelerating sisomicin production during idiophase.
A study on strain improvement by protoplast fusion between amylase secreting yeast and alcohol fermenting yeast - III. Isolation and characterization of fusant between S. diastaticus and C. tropicalis
Microbiology and Biotechnology Letters, volume 14, issue 5, 1986, Pages 359~363
S. diastaticus hydrolysised
-1.4 linkage of the starch and was known fermenting yeast strain, but poorly hydrolized
-1.6 linkage of the starch. To improve the starch fermentation ability of yeast, we tried that protoplast fusion between S. diastaticus and C. tropicalis and finally two starins of fusant (FPDC42, FPDC43) were obtained. C. tropicalis well hydrolysis both
-1.6 linkanges in the starch. The protoplast of parental auxotrophic cells were fused in the presence of 10mM CaCl
and 35% of polyethylene glycol (M. W. 4,000). The fusion frequency was 10
. Properties of the fusants(genetic stability, assimilation of carbon sources, random spore formation, copper resistance, NaCl tolerance, DNA content, cell size and growth rate) were investigated.
A study on strain improvement by protoplast fusion between amylase secreting yeast and alcohol fermenting yeast - IV. Alcohol and pullulanase productivities of fusant between S. diastaticus and C. tropicalis -
Microbiology and Biotechnology Letters, volume 14, issue 5, 1986, Pages 365~369
The activity of glucoamylase and pullulanase, properties of glucoamylase and ethanol productivities of fusants were studied. Glucoamylase and pullulanase activity of fusants were higher than parents. The optimal pH and temperature of glucoamylase of fusants were very similar to the those produced by S. diastaticus. In alcohol fermentation. fermenting ability and fermentation rate of fusants were higher and faster than either of its parental strain. The maximum of alcohol yield in 15% of liquefied potato starch was 7.8% (v/v)
Impurities formed from ethanol fermentation process among different materials and it′s effective separation in large scale
Microbiology and Biotechnology Letters, volume 14, issue 5, 1986, Pages 371~376
This study to elucidate concentrations of impurities such as methanol and fusel oil formed during fermentation process among the different materials and has been applied technical seperation for processing improvement by continuous distillation of super-allospas type. Methanol was formed high concentrations of cutting dried sweet potato and tapioca in order among the different materials during fermentation process. n-Propanol oil was formed high concentration of rice, cutting dried sweet potato, corn, naked barley and tapioca in order among the those materials. I-Buthanol showed high concentration of tapioca, corn, rice, cutting dried sweet potato and naked barley in order and isoamyl-alcohol showed high concentration of tapioca, rice, cutting dried sweet potato, corn and naked barley in order. Using the continuous distillation of super-allospas type, the following are collection ratios of n-propanol, iso-butanol, n-butanol and iso-amylalcohol: 37.9％, 28.6％, 37.4％, and 56.1％ when 78.25％ (v/v), 68.54％ (viv), 50.0％ (viv), and 50.0％ (v/v) alcohol are used, respectively. Fusel oil and bad alcohol put into the recovery column and then seperated directly by side cut of fusel oil partially from plate of tower bottom after concentration again. Extra impurities seperated by fusel oil seperator when 20 ％ (v/v) alcohol adjusted with water.
Isolation and identification of cellulolytic Actinomycetes
Microbiology and Biotechnology Letters, volume 14, issue 5, 1986, Pages 377~383
About 300 cellulolytic actinomycetes isolated from soils were tested for their cellulase activities estimated by means of filter paper swelling and carboxymethyl cellulose saccharifying activity. Then, 16 isolates which had shown relatively high levels of CMCase activity were selected and examined for their abilities of
-glucosidase production. Among them strain No. 109 was found to have highest level of intracellular
-glucosidase, and selected for the further studies. In this paper, the cultural, morphological and physiological properties, and cell wall composition of strain No. 109 were described in relation to the taxonomic status of this actinomycete. Based on the results obtained in these experiments strain No. 109 was identified to be a similar species to Streptomyces tanashiensis.
The Relationship between Hydrogenase and Nitrogenase for Hydrogen Evolution in Rhodopseudomonas sp. KCTC 1437
Seol, Won-Gi ; Kho, Yung-Hee ;
Microbiology and Biotechnology Letters, volume 14, issue 5, 1986, Pages 385~389
Both hydrogenase and nitrogenase were found to be involved in hydrogen evolution independently in Rhodopseudomonas sp. KCTC 1437. The hydrogen formation in this bacterium was independent on light illumination and presence of N
After establishment of conditions to measure the amount of hydrogen evolved by each of the enzymes in vivo, the several factors affecting on the hydrogen evolution, e.g. presence of gases (
), C/N ratio, were investigated, Hydrogenase was less inhibited than nitrogenase under
and was active independent on the presence of
which were the strong inhibitor of nitrogenase. Besides, the hydrogenase activity was increased after incubation with
. And it was verified that this bacterium consume hydrogen and photoreduce
by hydrogenase. From above results, it is concluded that hydrogenase in Rhodopseudomonas sp. KCTC 1437 can produce hydrogen under more favorable condition that nitrogenase.e.
Studies on the morphological and physiological characteristics of isolated strains from rotting ginseng
Microbiology and Biotechnology Letters, volume 14, issue 5, 1986, Pages 391~397
Three kinds of microoganisms were isolated and identified from the ginseng and ginseng products to research the properties of the molds which spoil the ginseng and ginseng products. The results obtained were as follows: (1) The predominant strains on ginseng products were Aspergillus sp., Penicillium sp.-A and Penicillium sp.-B. These predominant fungi deteriorated ginseng products exclusively, (2) Aspergillus sp. showed the greatest mycelial growth at
and its optimum pH was 5, meanwhile Pencillium sp. showed the greatest mycelial growth at
and its optimum pH was 3. (3) The growth of the isolated strains was stimulated with the increase in the concentration of saponin at the lower concentration, meanwhile it was inhibited at 1.0% concentration of saponin.
A Novel saccharification method of uncooked concentrated corn starch using an agitated bead reaction system
Microbiology and Biotechnology Letters, volume 14, issue 5, 1986, Pages 399~405
Corn starch was saccharified without cooking in an agitated bead reaction system. Uncooked corn starch was effectively hydrolyzed even at the concentration as high as 39%(w/v). After 24 hours. the extent of saccharification reached at 92%, which corresponds glucose concentration of 425g/L. Fed-batch feeding of starch was more effective than batch feeding for saccharification of uncooked corn starch. The composition of hydrolysated of uncooked starch was analyzed. which was composed of 95% glucose, 0.7% of maltose, and 4.5% of high saccharide, similar with that of cooked starch. The hydrolysate can be successfully utilized for HFCS manufacture. The starch liquefying and saccharifying enzyme was relatively stable even be the physical impact of the attrition-milling media. The enzyme stabilizer,
, played an essential role in preventing the enzyme deactivation caused by the physical impact.
Enhancing mechanism of the saccharification of uncooked starch in an agitated bead reaction system
Microbiology and Biotechnology Letters, volume 14, issue 5, 1986, Pages 407~413
In an agitated bead reaction system, the enzymatic saccharification of uncooked starch was substantially enhanced. The enhancement mechanism was investigated front the view of the structural aspect of starch. The mechanical impact caused by the movement of the attrition-milling media resulted neither the destruction of microcrystalline structure nor the fragmentation of starch granule. instead, the most distinct phenomenon was the swelling of starch granule up to about 2.5 times, and the swelling mechanism was not similar with that caused by cooking. However, in the case of the enzyme addition in the attrition coupled reaction system, the swollen starch was easily fragmented into the large number of small particles by the synergistic action of the enzyme and milling-media. The exposed surface area of the fragmented particles plays the major role in enhancing the saccharification. The saccharification rate was quite different depending on the source of starch, the reason was discussed in terms of the granular structure of uncooked starches.
Alcohol Fermentration of Naked Barley without Cooking
Microbiology and Biotechnology Letters, volume 14, issue 5, 1986, Pages 415~420
Alcohol fermentation of uncooked naked barley was carried out by the combined action of the maceration enzyme from black Aspergillus niger and the glucoamylase from Rhizopus sp. The combined enzyme preparation was found to be effective in maceration and saccharification of the raw naked barley starch. The Hydrolysis rate measured by the amount of glucose liberated reached more than 70% at pH 4.5 and 3
after 76 hrs. For alcohol fermentation without cooking, the naked barley mash of 18% initial total sugar was pretreated with concentrated sulfuric acid (0.15 weight % of the mash volume) at 55
for 2hr, and used for alcohol fermentation. A simultaneous saccharification and fermentation was carried out at pH 4.8 and 3
for 96 hrs. Under this fermentation condition, 3.5% increase in alcohol yield together with 2.0% increase in alcohol concentration were obtained when compared with the conventional cooking fermentation.
Effect of the amino acid mixture on freeze-drying and preservation of Lactobacillns casei YIT 9018
Microbiology and Biotechnology Letters, volume 14, issue 5, 1986, Pages 421~426
This study was attempted to find out effective storage methods of Lactobacillus casei YIT 9018, industrial strain for fermented mil k production, without severe bacterial death and activity deteriorations. The cryoprotective effect of the ammo acid mixture consisting of glycine and DL-g1utamic acid on the test strain were examined and also compared with those other protectants already reported. The apparent protective effect by the amino acid mixture was observed to controls. Both glycine and DL-glutamic acid prevented the freezing death of test strain and his effect of 1. casei YIT 9018 had reached stationary stage in MRS-broth 18h after inoculation. Cells harvested from stationary stage were most resistant to freezing damage. The viability of the test strain was affected by rehydration media and the recovery of viable cells was increased about threefold when amino acid mixture was used for rehydration. The presence of non-fat milk solid (NFMS), sucrose and lactose in amino acid mixture increased viability of the test strain up to 85％. In this case, optimal concentrations of NFMS, sucrose and lactose were 10％, 7.5-10％, 7.5-10％, respectively.
Genetic regulation for the biosynthesis of glutamate family in Corynebacterium glutamicum
Kim In-Ju ; Kyung Hee Min ; Sae Bae Lee ;
Microbiology and Biotechnology Letters, volume 14, issue 5, 1986, Pages 427~432
The regulation of three ammonia assimilatory enzymes, GDH (glutamate dehydrogenase), GS (glutamine synthetase) and GOGAT (glutamate synthase), has been examined in C. glutamicum. Three kinds of arginine auxotrophs blocked in each step of arginine biosynthetic pathway from glutamate were selected as arg 5, arg 6, arg 8. Histidine and tryptophan auxotrophs were also selected because histidine and tryptophan repressed GS biosynthesis in E. coli. These strains were cultured on the media containing nitrogen-excess and limited conditions, to compare the specific activities of
), GDH, GS, GOGAT from the cell-free extracts. These results showed that enzyme levels of
and GDH from 3 kinds of arginine auxotrophs, histidine and tryptophan auxotrophs in nitrogen-excess condition and those of GS and GOGAT in nitrogen limited condition were increased compared with opposite condition. The tryptophan and histidine auxotrophs showed higher level of glutamate and glutamine than parental strains and other mutants. it is assumed that the higher levels of
and GDH from mutants in nitrogen-excess condition promoted the accumulation of glutamate and glutamine in fermentation broth. The inhibition of GS activities by ADP suggested that GS is regulated by energy charge in C. glutamicum. The results with histidine, tryptophan, glycine, alanine, serine and GMP implied that a system of feedback inhibition were effective. The GDH, GS and GOGAT biosynthesis in culture broth was markedly repressed by the nature and kinds of available nitrogen sources such as tryptophan, proline, glycine, alanine, serine and tyrosine.