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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 15, Issue 6 - Dec 1987
Volume 15, Issue 5 - Oct 1987
Volume 15, Issue 2 - Apr 1987
Volume 15, Issue 1 - Feb 1987
Volume 15, Issue 4 - 00 1987
Volume 15, Issue 3 - 00 1987
Selecting the target year
Cloning of the Pullulanase Gene from Klebsiella pneumoniae NFB-320 in Esherchia coli
Yu, Ju-Hyun ; Chung, Kun-Sub ; Kong, In-Soo ; Lee, Jung-Kee ;
Microbiology and Biotechnology Letters, volume 15, issue 3, 1987, Pages 141~141
Pullulanase gene of Klebsiella pneumoniae NFB-320 has been cloned on plasmid pBR 322. Results showed that hybrid plasmid pYKL 451 contained 10 kb fragment of K. pneumoniae DNA and maintained stably after several transfer. The pullulanase produced by recombinant Esherichia coli (pYKL 451) was located mainly in cellular fraction.
Intergeneric Protoplast Fusion between Hansenula anomala var. anomala and Saccharomyces cerevisiae
Chung, Kun-Sub ; Koo, Young-Jo ; Shin, Dong-Hwa ;
Microbiology and Biotechnology Letters, volume 15, issue 3, 1987, Pages 145~145
In order to improve the growth of starch-utilizing yeast, Hansenula anomala var.anomala, studies were conducted on the protoplast fusion between four kinds of mutants of H. anomala var. anomala and Saccharomyces cerevisiae. Two fusants were finally selected by comparison of growth. Properties of fusants-cell volume, DNA content, amylolytic activity and fermentation products-were investigated, These fusants had properties that is originated from two fusion partners. The genetic stability of these fusants were very stable during successive subculture in complete dextrose medium.
Microbial Characterization of Gajami Sik-hae Fermentation
Moussa, Souane ; Kim, Young-Bae ; Lee, Cherl-Ho ;
Microbiology and Biotechnology Letters, volume 15, issue 3, 1987, Pages 150~150
The microflora of the raw materials and fermented product of Gajami sik-hae and the influence of spices on the microbial growth were studied. Lactobacillus and Leuconostoc spp. were found to be the dominant microflora of the product fermented at
in spite of their rareness in the raw materials. The secondary flora were constituted mainly by Bacillus spp. Garlic juice were found to inhibit the growth of Bacillus, Micrococcus, Pseudomonas and Aspergillus species, while Lactobacillus and Leuconostoc spp. were not effected by it. The number of total acid producing and proteolytic bacteria decreased when garlic content in the gajami sik-hae was increased from 2.5% to 5%. The other spices, red pepper and ginger showed no broad and significant antimicrobial activity.
Physiological Characteristics of Methionine Auxotroph Escherichia coli A10-28
Lee, Young-Gil ; Kim, Kwang-Hyeon ;
Microbiology and Biotechnology Letters, volume 15, issue 3, 1987, Pages 158~158
Methionine auxotrophic mutant for the bioassay of methionine and vitamine
was selected from Escherichia coli by U.V. irradiation, and the physiological characteristics of the mutant were investigated. The mutant was grown in proportion to the concentration of methionine in minimal medium up to 100 ng/ml methionine. However, any amino acids and any intermediates related in methionine biosynthesis were not effective on the mutant cell growth. The mutant in the minimal medium containing 40 ng/ml methionine and 1
g/ml folic acid, was grown in proportion to the range from 0 to 400 pg/ml vitamin
Lactic Acid Fermentation of Soymilk by Mixed Cultures of Lactobacillus acidophilus and Kluyveromyces fragilis
Yu, Ju-Hyun ; Lew, In-Deok ; Park, Chung-Kil ; Kong, In-Soo ;
Microbiology and Biotechnology Letters, volume 15, issue 3, 1987, Pages 162~162
Lactobacillus acidophilus (KFCC 12731) and Kluyveromyces fragilis (KFCC 35458) were inoculated together in soymilk, and then growth characteristics, acid production and the conditions suitable for acid production were investigated. L. acidophilus produced more acid and the rate of acid production was more rapid when this organism was incubated with K. fragilis in soymilk than when it was incubated singly. Studying the conditions suitable for lactic acid production in soymilk, optimum acid production by the mixed cultures of L. acidophilus and K. fragilis was achieved with a temperature of 35-
a 1:5-1:9 (O.D.660) ratio of L. acidophilus to K. fragilis at inoculum, a 0.5-1.0% level of sucrose fortification or a 1.5% level of skim milk fortification and a culture time of 24 hr. Under these conditions the amount of acid produced by the single culture of L. acidophilus and the mixed cultures of L. acidophilus and K. fragilis were 0.17% and 0.43%, respectively, in soymilk, 0.20% and 0.80%, respectively, in soymilk fortified with 1.0% level of sucrose. These indicate that the amount of acid produced by mixed culture is about 2.53-fold greater in soymilk and about 4.0-fold greater in soymilk fortified with 1.0% level of sucrose than that produced by the single culture of L. acidophilus. The amount of acid produced by the single culture of L. acidophilus and the mixed culture with K. fragilis in soymilk fortified with 1.5% level of skim milk were 0.77% and 0.80%, respectively. The amount of acid produced by the mixed culture with K. fragilis was greater than that produced by the single culture of L. acidophilus only in soymilk fortified with lower levels of skim milk than 1.5%, beyond which, the amount of acid produced by the single culture of L. acidophilus and the mixed culture with K. fragilis was demonstrated to be almost same level.
The Conversion of D-Xylose to Ethanol by Immobilized Pachysolen tannophilus
Oh, Jong-Won ; Choi, Ho-Joon ; Pyun, Yu-Ryang ;
Microbiology and Biotechnology Letters, volume 15, issue 3, 1987, Pages 170~170
Pachysolen tannophilus cells were immobilized in calcium alginate gel to ferment D-xylose on a continuous basis. Ethanol productivity of immobilized cells was enhanced by activation for 48 h. To minimize the resistance of diffusion, the bead size less than 2 mm in diameter was desirable. The immobilized yeast cells were examined in a packed bed reactor system. The highest ethanol yield which was corresponding to 89% of theoretical yield was obtained with the xylose concentration of 25 g/l and dilution rate of 0.48
The activity of immobilized cells was maintained for 20 days during continuous operation in packed-bed reactor.
Culture Condition for Inulase Production by Pseudomonas species
Lee, Tae-Kyung ; Sung, Ha-Chin ; Choi, Yong-Jin ; Yang, Han-Chul ;
Microbiology and Biotechnology Letters, volume 15, issue 3, 1987, Pages 176~176
A bacterial strain capable of producing high level of extracellular inulase was isolated from soil samples, and the characteristics of the isolated strain No 65, supported the assignment of the strain to a species of Pseudomonas. The maximum inulase production (about 6.6 units/ml) was obserbed in the culture medium containing 1% inulin, 1.5% corn steep liquor, 0.8%
with initial pH value of 7.0 when the Pseudomonas was cultured at
for about 60 hrs with rotary shaking.
Isolation and Properties of a Aminopeptidase from Streptococcus cremoris
Hwang, In-Kyu ;
Microbiology and Biotechnology Letters, volume 15, issue 3, 1987, Pages 184~184
A aminopeptidase was isolated from the cell-free extract of Streptococcus cremoris H61 by a method that included ammonium sulfate fractionation DEAE-cellulose chromatography and Sephadex G-150 gel filtration. The enzyme showed a wide range of substrate specificity on di or tripeptides but hardly hydrolyzed carbobenzoxylpeptides. The enzyme exhibited optimum pH around 6.5 and was stable up to
It was activated by
and inhibited by EDTA, 1,10-phenanthroline and bestatin.
Production and Partial Purification of Thermostable
-Amylase from Streptomyces sp. HA-40
Yi Dong-Heui ; Kwon, Tae-Jong ; Chung Ho-Kwon ;
Microbiology and Biotechnology Letters, volume 15, issue 3, 1987, Pages 190~190
A strain of Streptomyces sp. (HA-40), isolated from soil, was able to produce a thermostable
-amylase. And the conditions for production and partial purification procedure were investigated. For production of the enzyme, starch and peptone was favorable as carbon and nitrogen source, respectively. And the enzyme production was promoted by the addition of
but depressed remarkably by
Optimal initial pH and temperature for the enzyme formation was pH 7.0 and
respectively. The production of the enzyme reached at maximum in 2 days cultivation in the medium containing 1% starch, 0.2% peptone, 0.1%
and 0.05% NaCl. The enzyme was partially purified about 42 folds from cultured filtrate by 80% methanol precipitation, DEAE-cellulose and Sephadex G-100 column chromatography with overall yield of 43%.
Fermentability of Partially Saccharified Uncooked Starch in Attrition-Coupled Reaction System
Jo, Ku-Hyung ; Lee, Sang-Ho ; Lee, Yong-Hyun ;
Microbiology and Biotechnology Letters, volume 15, issue 3, 1987, Pages 196~196
The suitability of partially or completely saccharified uncooked naked barley in an agitated bead reaction system as a substrate for alcoholic fermentation was examined. The fermentability of complete hydrolysate of uncooked barley was superior to that of obtained from cooking process in terms of fermentation rate and yield, especially in earlier stage of fermentation. The partially hydrolyzed uncooked starch over 4 hours-milling in this system was also found to be suitable as a fermentation substrate due to the swelling and fragmentation of granular structure. Simultaneous hydrolysis-fermentation in an agitated bead reaction system was unsuccessful due to the destruction of yeast cell. The growth of contaminant during fermentation was severely restricted due to the loss of viability of cell caused by the physical impact of milling media.
Purification and Catalytic Properties of Cephalexin Synthesizing Enzyme from Acetobacter turbidans
Kang Sang-Mu ; Son Kwang-Hui ; Kim Jung-Hoe ; Kim Deog-Jung ; Kim Young-Jun ;
Microbiology and Biotechnology Letters, volume 15, issue 3, 1987, Pages 203~203
Using Acetobacter turbidans enzyme, enzymatic synthesis of cephalexin (CEX) from D-
phenylglycine methylester (PGM) and 7-amino-3-deacetoxycephalosporanic acid (7-ADCA) was attempted. This enzyme was partially purified through ammonium sulfate fractionation, DEAE and CM-column chromatography, and Sephacryl S-200 gel filtration. The purified enzyme migrated as a single band on a polyacrylamide disc gel electrophoresis. This enzyme showed its maximal synthetic activity at pH 6.0 and
and its activation energy was determined as 9.19 Kcal/mol. An unique kinetic pattern that the maximal conversion achieved was greatly affected by molar ratio of two substrates and the amount of enzyme loaded, was observed. The maximal conversion of 7-ADCA to CEX was 85% at the molar ratio of PGM to 7-ADCA, 2-4.
L-Phenylalanine Production from trans-Cinnamic Acid with Rhodotorula glutinis KCTC 1209
Kho, Yung-Hee ; Lee June-Woo ; Chung, Ho-Kwon ;
Microbiology and Biotechnology Letters, volume 15, issue 3, 1987, Pages 209~209
L-Phenylalanine production from trans-cinnamic acid through the reverse reaction of phenylalanine ammonia-lyase (PAL; EC 22.214.171.124) from Rhodotorula glutinis KCTC 1209 was investigated. The formation of the PAL enzyme reached its maximum at 20-22 hrs of cultivation under optimized culture condition. The enzyme activity was maintained stably for a relatively long period during cell cultivation by the addition of L-phenylalanine and L-isoleucine. The conversion yield of trans-cinnamic acid to L-phenylalanine by the intact cells was about 5% after optimization of the enzyme reaction parameters.