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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 15, Issue 6 - Dec 1987
Volume 15, Issue 5 - Oct 1987
Volume 15, Issue 2 - Apr 1987
Volume 15, Issue 1 - Feb 1987
Volume 15, Issue 4 - 00 1987
Volume 15, Issue 3 - 00 1987
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Studies on Yeast Vector System for Eukaryotic Gene Cloning -Improvement of Chromosomal Autonomous Replicating Vector of Yeast, YRp7-
Ki, Woo-Kyung ; Yun, Sang-Jung ; Seok, Kil-Yong ; Lee, Young-Choon ; Song, Jae-Young ;
Microbiology and Biotechnology Letters, volume 15, issue 4, 1987, Pages 217~217
A series of plasmid vectors designated as pRE18, pRN18, YRN008, YREp were constructed for improvement of those vector containing autonomous replicating segment (ARS) from chromosomal DNA of Saccharomyces cerevisiae. The improved vectors, pRE18 and pRN18 were constructed by the insertion of ARSl fragment to EcoR I or Nde I site of pUC18 and those vectors showed high stability in S. cerevisiae and have multicloning site for cloning and selection marker. YRN008 for antibiotic selection in S. cerevisiae or Escherichia coli was constructed as a vector containing pUC13, G418 gene derived from Tn603 and ARSl trpl.
Studies on Yeast Vector System for Eukaryotic Gene Cloning -New Cloning Vector Containing Autonomous Replicating Segment from Candida tropicalis-
Ki, Woo-Kyung ; Seok, Kil-Yong ; Yun, Sang-Jung ; Song, Jae-Young ;
Microbiology and Biotechnology Letters, volume 15, issue 4, 1987, Pages 224~224
Autonomous replicating segment (ARS) derived from Candida tropicalis IFO 0518 has been newly cloned in Saccharomyces cerevisiae YNN27 with YIp5. A new plasmid which named as pIKS was isolated from S. cerevisiae YNN27 transforment. The cloned gene contained a 8kb-Sal I fragment of C. tropicalis DNA (C. Sars). We have compared the stability of pIKS with that of YRp7 in S. cerevisiae YNN 27 and established a restriction cleavage sites with various endonucleases.
Isolation of Auxotrophic Mutants in Ganoderma applanatum
Park, Young-Do ; Yoo, Young-Bok ; Cha, Dong-Yeul ; Chang, Moo-Woong ; Lee, Jae-Sung ;
Microbiology and Biotechnology Letters, volume 15, issue 4, 1987, Pages 230~230
Mycelia of Ganoderma applanatum were irradiated with ultra violet-light to obtain auxotrophic mutants. When mycella were treated with ultra violt-light for 10 min., the highest proportion of auxotrophic mutants was obtained and survival rate of mutants was 6.5%. 5 strains were selected as auxotrophs. Among them 3 strains were uncleic acid base and components requiring, 2 strains were amino acid requiring.
Protoplast Formation and Reversion of Ganoderma applanatum
Park, Young-Do ; Yoo, Young-Bok ; Park, Young-Hwan ; Chang, Moo-Woong ; Lee, Jae-Sung ;
Microbiology and Biotechnology Letters, volume 15, issue 4, 1987, Pages 234~234
This study was executed to investigate proper conditions for protoplast formation and reversion from Ganoderma applanatum. The highest isolated protoplast yields was
cells 1 ml by using a combined enzyme system containing Novozym 234, Cellulase Onozuka R-10 and
-glucuronidase 10 mg/ml each. The most effective osmotic stabilizer for protoplast was sucrose to support release and maintenance. The optimal reaction time of mycellum with lytic mixtures was 2 hours at 120 strockes/min. When mycellum was cultured for 3 days, protoplast releasing was the most effective. Released protoplast and cell wall were identified with transmission electron microscope. Osmotic stabilizer with 0.6M sucrose was the most effective for protoplast reversion and GCM was suitable as the protoplast reversion medium. Reversion frequency was obtained 1.04-7.76%.
Hydrophobic Properties of a Regularly Arrayed Cell Surface Properties of Lactobacillus acidophilus
Chung, Yung-Gun ; Ahn, Jang-Yeon ; Son, Dong-Hwa ; Kwon, Oh-Jin ;
Microbiology and Biotechnology Letters, volume 15, issue 4, 1987, Pages 241~241
The hydrophobic natures of the cells of Lactobacillus acidophilus strains, with or without the regular array (RA) on the cell wall, were examined by partitioning of bacteria at hydrocarbon (n-octane or p-xylene): water interfaces. No relationship, however, was observed between the presence or absence of the RA and the hydrophobicity of the cells. Removal of RA by the treatment with guanidinehydrochloride gave little changes in the hydrophobicity, suggesting that the cellular hydrophobicities of the organisms are not dependent on the RA. RA proteins from these strains tested were similar in amino acid compositions, except two strains, VPI 1799 and NCTC 2949, in which contain more glycine and less hydrophobic amino acid residues in their RA. The calculated average value of hydrophobic energy of the RA proteins based on their amino acid composition ranged from 400 to 700 cal/mole. These values are lower than those found for RA protein of L. buchneri and several bacterial enzyme proteins. The results described above strongly suggest that the cellular hydrophobicity of L. acidophilus is not due to RA protein, but to carbohydrate polymers.
Continuous Alcohol Fermentation Using Immobilized Growing Yeast Cells
Ryu, Beung-Ho ; Nam, Ki-Du ;
Microbiology and Biotechnology Letters, volume 15, issue 4, 1987, Pages 248~248
Continuous alcohol production by Saccharomyces cerevisiae IFO 1-84, immobilized in Na-alginate was studied in a packed column reactor using synthesized glucose medium as a feed. The gel beads entrapping small amount of cells were cultured in a column reactor for 60 h, and then the glucose concentration was increased stepwise from 50 to 208g/l in the feed running the reactor for 340 h. By this stepwise feeding system, the average cell concentration reached
cells/ml bead. The maximum alcohol productivity was 17.48g/l, h with 0.365g/g
of yield at D=0.23
Interaction between Lactobacillus acidophilus and Kluyveromyces fragilis on the Metabolism of Galacto-oligosaccharides in Soymilk
Lew, In-Deok ; Yu, Ju-Hyun ;
Microbiology and Biotechnology Letters, volume 15, issue 4, 1987, Pages 253~253
The mechanism involved in the enhanced growth and lactic acid production of Lactobacillus acidophilus (KFCC12731) mixed with Kluyveromyces fragilis (KFCC35458) in soymilk was investigated. K. fragilis was grown in soymilk and the resulting culture filtrate was added to the culture of L. acidophilus and the effect on the growth and acid production was observed. It was found that K. fragilis produced enzymes which can convert sucrose, raffinose, and stachyose, common sugars of soymilk, into glucose and fructose which can be fermented by L. acidophilus, and in turn stimulated growth and acid production of the latter.
Vitamin-Producing Microorganisms Isolated from Cornmeal Fermented at 45
Dyer, Randy L. ; Fields, Marion L. ; Chung, Hee-Jong ;
Microbiology and Biotechnology Letters, volume 15, issue 4, 1987, Pages 261~261
The aim of this research was to evaluate which microorganisms were present in natural lactic acid fermentation of cornmeal that produced riboflavin and vitamin
and grew at
Microorganisms isolated were: Streptococcus faecalis, Lactobacillus delbrueckii, Bacillus licheniformis, Agrobacterium sp., and Aeromonas hydrophila. All these isolates grew at
Some strains of B. licheniformis, S. faecalis and L. delbrueckii grew at
Purification and Properties of Extracellular
-Xylosidase from Thermophilic Alkalophilic Bacillus sp. K-17
Sung, Nack-Kie ; Kang, In-Soo ; Chun, Hyo-Kon ; Akiba, Teruhiko ; Horikoshi, Koki ;
Microbiology and Biotechnology Letters, volume 15, issue 4, 1987, Pages 267~267
-xylosidase was purified 22-fold from culture filtrate of thermophilic alkalophilic Bacillus sp. K-17 by ammonium sulfate precipitation, DEAE-Sephadex A-50 chromatography and Sephadex G-100 gel filtration. The molecular weight was calculated to be 51,000 by SDS gel electrophoresis. The enzyme had a pH optimum for activity at 7.0 and retained appreciable activity at pH 10.5, and its stability range was pH 6.0-10.0. The optimal temperature of enzyme was
and its activity was completely inactivated by incubation at
for 10 min. The Michaelis constant for p-nitrophenyl-
-D-xylopyranoside was calculated to be 0.45 mM.
-Xylosidase could degrade xylooligosaccharide, produced from xylan by the action of xylanase, to xylose.
Isolation and some Properties of D-Xylose Isomerase Producing Alkalophilic Bacillus sp.
Kwon, Ho-Joeng ; O, Pyong-Su ;
Microbiology and Biotechnology Letters, volume 15, issue 4, 1987, Pages 273~273
A D-xylose isomerase producing bacterium was isolated from soil using D-xylose as a sole carbon source in alkaline medium containing 1% sodium carbonate. This strain could produce D-xylose isomerase when it was grown in the medium containing xylose, xylan, and xylan containing material such as wheat bran. The culture pH also had a great influence on cell growth and enzyme production; high yield of D-xylose isomerase was observed in alkaline media and the induction of the enzyme was faster at pH 10.2 than at pH 7.2. The enzyme was most active at pH 7-10, and was stable at pH 6.0 to 11.0 incubated at
for 30 min.
Studies on Extracellular Protease from Saccharomycopsis lipolytica -Condition of Enzyme Production-
Kang, Kook-Hee ; Bae, In-Hyu ; Lee, Chun-Hwa ;
Microbiology and Biotechnology Letters, volume 15, issue 4, 1987, Pages 279~279
Nutrional requirement and cultural condition for the production of extracellular protease by Saccharomycopsis lipolytica, a dimorphism yeast, were investigated. The culture broth giving maximum protease yield was found to consist of 5% glucose and 2% sodium acetate as carbon source, 2% casein as a nitrogen source, and 0.001% ferric chloride and 0.3% potassium phosphate, 0.1% magnesium sulfate as mineral source. Optimal initial pH of culture broth was 9.0 and the enzyme excretion in the culture broth usually reaches a maximum after 36 hours of cultivation at
It is necessary to limit the culturing time, because the protease activity in the culture broth tend to decrease after excessive culturing.
Studies on Extracellular Protease from Saccharomycopsis lipolytica (Candida lipolytica) -Purification and Properties of Enzyme-
Bae, In-Hyu ; Kang, Kook-Hee ;
Microbiology and Biotechnology Letters, volume 15, issue 4, 1987, Pages 286~286
An extracellular protease produced by Saccharomycopsis lipolytica was purified to homogeneity by procedures including ammonium sulfate precipitation, DEAE-cellulose ion exchange chromatography, Sephadex G-100 gel filtration and DEAE-Sephadex A-50 ion exchange chromatography. The purifying procedures resulted in 67-fold purification with the overall yield of 26% and the purified enzyme had a specific activity of about 2670 unit per mg protein. The optimum pH of its caseinolytic activity was 9.0, and this activity was completely inhibited by phenylmethylsulphonyl fluoride and ethylenediaminetetraacetate but not inhibited by dithiothreitol, o-pnenanthroline, indicating that it is a serine protease. The molecular weight of the enzyme was estimated to be about 30,000
1000 by the gel filtration on Sephadex G-100.
Studies on the Selective Enumeration Medium for the Frozen-damaged Cells of Lactobacillus casei YIT 9018
Kim, Jong-Myung ; Baek, Young-Jin ; Kim, Hyun-Uk ;
Microbiology and Biotechnology Letters, volume 15, issue 4, 1987, Pages 294~294
In order to enumerate selectively the frozen-damaged cells of Lactobacillus casei YIT 9018, the selective agar medium (Lactobacillus SF Agar) has been studied. Lactobacillus SF Agar contained every ingredients of MILSA except peptone, tryptone, soytone with the addition of 0.02% of
adjusted to pH 5.0.
Assesment of Osmotic Stabilizer to be used on Protoplast Formation
Cho, Bo-Yeon ; Bae, Moo ;
Microbiology and Biotechnology Letters, volume 15, issue 4, 1987, Pages 299~299