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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 15, Issue 6 - Dec 1987
Volume 15, Issue 5 - Oct 1987
Volume 15, Issue 2 - Apr 1987
Volume 15, Issue 1 - Feb 1987
Volume 15, Issue 4 - 00 1987
Volume 15, Issue 3 - 00 1987
Selecting the target year
Identification and Salt Requirement of Halophilic Bacteria isolated from Korean Salt-Fermented Sen Foods.
Bae, Moo ; Song, Kyoung-Sook ;
Microbiology and Biotechnology Letters, volume 15, issue 5, 1987, Pages 301~305
Moderate halophilic bacteria isolated from several kinds of salted and fermented sea foods (jeotkal) collected from commercial market were identified and investigated on their selt requirements. It was confirmed that the isolates were dominantly moderate halophiles according to their NaCl requirement to grow. And their salt requirements in their growth have been examined for Na, K, Ni and mg ions. Among them, the most dominant and distinctive three strains in protease pro-duction have been examined and two of them identified to be halophilic Flavobacterium sp., and the other one to be halophilic Pseudomonas sp..1'heir optimum growth was observed at 3
and at 10 percent of NaCl.
Purification and Characterization of Extracellular Adenosine Deaminase from Streptomyces sp. J-350P
Microbiology and Biotechnology Letters, volume 15, issue 5, 1987, Pages 306~311
After series of purification by means of ammonium sulfate fractionation, the 1st and 2nd DEAE-Cellulose, DEAE-Sephadex A-50, and Sephacryl S-200 superfine gel filtration, the activity of extracellular adenine deaminase from Streptomyces sp. J-350P increased 1764 fold and the yield was 0.3% of original activity. The enzyme was stable at the pH range 6.5 to 8.5 and at up to 5
. The optimum pH and temperature of the enzyme were around 6.5 and 35
. The molecular weight ol the enzyme was estimated as 36, 000 by calibrated Sephacryl S-200 superfine column chromatography.
The Enzymatic Properties of Extracellular Adenine Deaminnse from Streptomyces sp. J-350P
Microbiology and Biotechnology Letters, volume 15, issue 5, 1987, Pages 312~318
The apparent Michaelis constant Km of extracellular adenine deaminase from Streptomyces sp. J-350P was 5.8
M. The activation energy or the enzyme was calculated from Arrhenius plots for adenine and the value was 3.13 Kcal/mole. The purine analogues, 6-chloropurine, 2,6-diaminopurine, 6-bromopurine, 4-aminopyrazolo[3,4-d] pyrimidine, 6-iodopurine, and 8-bromoadenine were substrates for the enzyme. 6-Dimethylaminopurine was a competitive inhibitor of the enzyme. The enzyme was inhibited by 0.1mM of Fe
, Ag+, and Hg
and 1 mM of
＇-dipyridyl, Penta-chiorophenol, and p-chloromercuribenzoate.
Construction of Plasmid Vectors for Zymomonas mobilis
Hwang, Duk-Ju ; Rhee, Sang-Ki ; Pack, Moo-Young ;
Microbiology and Biotechnology Letters, volume 15, issue 5, 1987, Pages 319~327
In order to develop useful plasmid vectors for Zymomonas cells, attempts were made to isolate natural plasmids from Z. mobilis ATCC10988. Among a few plasmids isolated, a small plasmid of 3.9 Kb size was chosen and designated as pZM3. By introducing the replication origin of pZM3 into pBR325, a hybrid plasmid vector of 8.4 Kb size, pHZ22, was constructed. This vector contained chloramphenicol resistant gene as a selectable marker and proved to be conjugally transmissible and stably maintained in Z. mobilis. Tetracycline resistant gene was isolated from RP4 and introduced into pHZ22 to make a new vector called pHZT224 of 10.7 Kb size. Through n series of experiments, it was evident that these plasmid vectors containing selectable markers of chloramphenicol and tetracycline resistance were shuttle vectors functional in Z. mobilis as well as E. coli.
Stabilities of Plasmid Vectors in Zymomonas mobilis
Microbiology and Biotechnology Letters, volume 15, issue 5, 1987, Pages 328~333
The stabilities of plasmid vectors in Zymomonas mobilis were tested in batch and continuous cultures. It was found that the growth of the host Zymomonas strain was greatly affected by the size of plasmids as well as the composition of nutrient media： the host cells grew taster when harboring plasmids of smaller sizes and in n non-selective medium. All the Zymomonas plasmid vectors containing antibiotics selective markers and Zymomonas replication origins could be maintained in a stable manner over 30 generations without being integrated into host chromosomes.
Enzymatic Conversion of Pyruvic Acid to Tryptophan tinted to Pyruvic Acid-Producing Microorganism
Microbiology and Biotechnology Letters, volume 15, issue 5, 1987, Pages 334~339
Enzymatic conversion of pyruvic acid produced by microorganism to tryptophan was investigated. A luminescent bacteria. Beneckea sp., was used for the production of pyruvic acid. As a source of tryptophanase which synthesizes tryptophan from pyruvic acid, indole and ammonia, whole cells of Enterobacter aerogenes ATCC 10031 were used directly in the reaction mixture. To increase the production of tryptophan, nonionic detergents and nonaqeous organic solvents were used ms reserviors of indole in the reaction mixture. In the case of nonionic detergents, TritonX-100 was very effective. When 1.5% of Triton X-100 was used, 7.7g/
of tryptophan was produced at 37
for 48hr. In the case of nonaqueous solvents, 8.7g/
of tryptophan was produced at 37
for 48 hr, when 10% of benzene was used. This amount of tryptophan corresponds to conversion of 48% of Indole and 36% of pyruvic acid, respectively.
Production of Ethanol from D-Xylose by Fusarium sp.
Microbiology and Biotechnology Letters, volume 15, issue 5, 1987, Pages 340~345
Microorganisms capable of utilizing D-xylose as a sole carbon and energy source were isolated to ferment D-xylose directly to ethanol. Among them, the strain, which showed the best ability to pro-duce ethanol, was selected and was identified as Fusarium sp. The optimal conditions for the pro-duction of ethanol were 8.0 of initial pH, 33
of temperature, and 2% of substrate concentration. Under this optimal condition, the following results were obtained : maximum ethanol concentration, 7.0g/
; ethanol yield, 0.35g of ethanol per g of D-xylose (68.6% of theoretical); biomass yield, 0.27g of dry biomass per g of D-xylose.
Cloning and Expression in Escherichia coli of a Cellulase Gene from Clostridium thermocellum
Microbiology and Biotechnology Letters, volume 15, issue 5, 1987, Pages 346~351
A cellulase gene of Clostridium themocellum was transferred to Escherichia coli by molecular cloning with pBR322. The gene was carried in a Hind III digested DNA sequence of about 1.8 kb. This Rind III fragment expressed activities on carboxymethyl cellulose (CMC) and on filter gaper in E. coli. The expression of clostridial cellulase gene in E. coli was studied and compared with the pro-ducts of cellulase genes in C. themocellum.
A New Restriction Endonuclease from Clostridium thermocellum
Choi, K.D. ; Kim, Kitae ; Yoo, Ook-Joon ;
Microbiology and Biotechnology Letters, volume 15, issue 5, 1987, Pages 352~355
The isolation and characterization of type II restriction endonuclease from Clostridium thermocellum ATCC 27405 were described. This enzyme (Cth I endonuclease) is an isoschizomer of Bcl I endonuclease recognizing 5'-TGATCA-3'. Cth I endonuclease requires MG
ion for its activity and is maximally active at PH 1.5 to 10.5 in the Presence of 0 to 10mM NaCl. Cth I endonuclease is heat stable and has an optimum temperature of 6
. The activity of Cth I enzyme is sensitive to dam methylation.
The Analysis of Some Factors Involved in Sisomicin Fermentation Based on Temperature Effects
Shin, Chul-Soo ; Lee, Sang-Han ; Kim, Sung-Uk ; Bok, Song-Hae ;
Microbiology and Biotechnology Letters, volume 15, issue 5, 1987, Pages 356~360
Effects of temperature on sisomicin fermentation were investigated. From the specific growth rates for logarithmic phase estimated at various temperatures, 8.2 kcal/g-mol was obtained as an activation energy for cell growth. It suggests that cell growth rate was limited by the internal diffusion layers for nutrients or oxygen caused by aggregated cells. Final antibiotic titer was decreased with in-creasing temperature, and it depended highly on the temperature to which cells were exposed during the logarithmic phase of growth. Temperature shifts during fermentation brought about an increase in antibiotic productivity.
Regulation of Sulfur Metabolism in Cephalosporium acremonium
Lee, Kyoung ; Park, Sang-Ho ; Lee, Jung-Joon ; Mheen, Tae-Ick ;
Microbiology and Biotechnology Letters, volume 15, issue 5, 1987, Pages 361~367
A DL-seleno-methionine resistant mutant, Cephalosporium acremonium MS-92 showed increased activities of sulfate and L-methionine uptake than the parent strain, and accumulated excess methionine and S-adenosylmethionine (SAM) intracellularly. And the sulfate uptake system was severely inhibited by L-cysteine. In crude enzyme extracts, the mutant MS-92 showed lower L-serine sulfhydrylase (identical with cystathionine
-synthase) activity than the parent. Also, cysteine desulfhydrylase activity, an index of intracellular L-cysteine concentration, of the mutant MS-92 was decreased by about 50% as com-pared with that of the parent. Thus, it was supposed that the mutant MS-92 should have n lower level of L-cysteine than the parent. In C. acremonium like A. nidulans, the enzymes related to the biosynthesis of methionine might be regulated by L-cysteine, but not by methionine or SAM.