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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 16, Issue 6 - Dec 1988
Volume 16, Issue 5 - Oct 1988
Volume 16, Issue 4 - Aug 1988
Volume 16, Issue 3 - Jun 1988
Volume 16, Issue 2 - Apr 1988
Volume 16, Issue 1 - 00 1988
Selecting the target year
A New Coloured Substrate for the Determination of
-Glucan Degrading Enzyme from Malt and Bacillus subtilis K-4-3
Microbiology and Biotechnology Letters, volume 16, issue 2, 1988, Pages 79~84
Dye materials and cross linking agents were used for the determination of
-glucanase activities. The objective of this study was to prepare the blue coloured substrates which are sensitive, specific and simple for the determination of
-glucanase in malt and Bacillus subtilis K-4-3 enzymes. This method is based on the principle of measuring colorimetrically the split product of coloured and cross linked substrate. The best coupling of dye stuff of
-glucan was cibacron blue 3G-A and the colour released can suitably be measured at 623nm. Optimal concentration of dye and cross linking agents was 1.5g and 1.25
under 0.1N NaOH. The sensitivity comparison proved that the stained
-glucan method is much more sensitive than the DNS method to determine reducing sugar released by the enzyme.
Efficiency Analysis of fermentation Process on Available Electron Balance
Lee, Kyung-Hee ; Kang, Woo-Kyu ; Kim, Byung-Woo ;
Microbiology and Biotechnology Letters, volume 16, issue 2, 1988, Pages 85~91
Energy efficiency of bacterial cell mass and product formation from cellulose using Ruminococcus albus and Ruminococcus flavefaciens with application of available electron balance were discussed. Values of true growth yield, η
and maintenance coefficient, m
, were estimated us-ing experimental data, and the results were compared with estimates obtained from theoretical ap-proach. Experimental values were similar in magnitude to theoretical values in
= 10.5 g cells/ mole ATP. Therefore,
values of Ruminococcus albus and Ruminocoecus flavefaciens were considered similar to 10.5 g cells/mole ATP.
Purification and Properties of a Cysteinylglycinase from Proteus mirabilis
Choi, Shin-Yang ; Yu, Ju-Hyun ; Hidehiko Kumagai ; Tatsrokuro Tochikura ;
Microbiology and Biotechnology Letters, volume 16, issue 2, 1988, Pages 92~97
Cysteinylglycinase was partially purified from Proteus mirabilis by consecutive procedure. The specific activity was increased about 16-fold to that of cell-free extract. The enzyme was found rather unstable on ammonium sulfate precipitation ann the precipitated enzyme protein became partially insoluble during dialysis. The precipitated enzyme was found to be solubilized by treatment of 4% Triton X-100 effectiviely, The optimum temperature and pH of the enzyme activity were 35
and 7.3, respectively. After heat treatment of the enzyme at 5
for 30 min, it lost the activity to 70%. The enzyme was stable at pH 7.0-8.0. The molecular weight of the cysteinylglycinase was found to be about 190,000 by Sephadex G-150 gel filtration. The enzyme was activated by the addition of Mn
ions. The maximal activation was obtained in preincubation with
ion for 30 min. The enzyme catalyzed the hydrolysis of various dipeptides and tripeptides. The Km and Vmax values for cysteinylglycine were 1.60 mM and 0.24 m unit/ mg, respectively.
Polysaccharide Production by a Gram Negative Facultatively Anaerobic Rod
Yoo, Jin-Young ; Koo, Young-Jo ; Shin, Dong-Hwa ; Chung, Dong-Hyo ;
Microbiology and Biotechnology Letters, volume 16, issue 2, 1988, Pages 98~104
A bacterial isolate FRI-33 which produces hydrophillic polysaccharide was identified and its cultural condition was investigated. FRI-33 was identified as Enterobacter agglomerans. The optimum cultural conditions for polysaccharide production were 3
, pH 5.7, using medium composed of glucose 25 g/
, peptone 2.0 g/
, yeast extract 0.5 g/
. The polysaccharide production after 72 hours was 8.41 g/
. The polysaccharide was composed of galactose (1.0 mole), xylose (1.5 mole), gluconodeltalactone (1.9 mole) and ribose (0.03 mole). The apparent viscosity of 1% polysaccharide solution was 504 mPa.s at 60 rpm and intrinsic viscosity was 45.80 d
Production of Single-Cell Protein from Starchy Material by the Fusant
Microbiology and Biotechnology Letters, volume 16, issue 2, 1988, Pages 105~110
The production of single cell protein using the amylolytic fusant obtained from cell fusion between Hansenula anomala and Saccharomyces cerevisiae was studied. The fusant12 strain was selected for single cell protein production from starchy materials among five fusants. Optimum nitrogen source and its concentration for the growth of fusant12 were ammonium sulfate and 0.1%, respectively. Optimum concentration of soluble starch and optimum pH of the basal medium were lord and pH 5.6, respectively. Autolysis of fusant12 was effectively carried out by addition of 5% (v/v) ethyl acetate to the cell suspension and liquidization for 30 min before incubation for 24 hr at 3
. Coculture of fusant12 and non-amylolytic yeast, Torulopsis candida YA-l5, resulted in the increase of the mass as compared to the monoculture of fusant12. The cell mass on tapioca medium was increased about 2.5 times as on soluble starch medium. The content of crude protein and nucleic acid of the dry cell were 39% and 5.8%, respectively.
Growth Inhibition of Enteropathogenic Escherichia coli
and Escherichia coli
by the Organic Acid Producing Bacteria
Microbiology and Biotechnology Letters, volume 16, issue 2, 1988, Pages 111~118
The growth inhibition of enteropathogenic Escheriohia coli
and Escherichia coli G
, causing the diarrhea in piglets, by the organic acid producing bacteria was studied in vitro. The metabolites of the organic acid bacteria, such as lactic acid, acetic acid inhibited the growth of E. coli
and E. coli G
in BL medium. The more the organic acid producing bacteria have ability to produce the organic acids, the higher these bacteria excelled the inhibitory efficacy against enteropathogenic E. coli. Among the strains examined, Lactobacillus casei Y and Streptococcus faecium C showed relatively strong growth inhibition against enteropathogenic E. coli.. When the organic acid producing bacteria and the enteropathogenic E. coli were incubated simultaneously in BL medium, bacteriostasis of E. coli was observed when the pH of BL culture was lowered to 5.0, and bacteriocidal effect was observed when the pH became Bess than 4.5, E. coli.
was more resistant to the organic acid bacteria than E. coli G
Biosynthetic Regulation and Enzymatic Properties of
-Glucosidase from Cellulomonas sp. CS 1-1
Microbiology and Biotechnology Letters, volume 16, issue 2, 1988, Pages 119~125
-Glucosidase of Cellulomonas sp. CS1-1 in cellular compartment was localized with cell-bound form while Avicelase and carboxymethylcellulase (CMCase) were appeared with extracellular enzyme. Cell growth on cellulose or CMC minimal broth was increased by glucose addition.
-Glucosidase production on cellobiose or CMC minimal broth was repressed by the addition of glucose. However, on CMC minimal broth, the enzyme production was specially stimulated by cellobiose addition.
-Glucosidase production was also induced by CMC, starcth and maltose compared with glycerol, arabinose, xylose and trehalose. From the above results, it was concluded that glucose effect on
-glucosidase biosynthesis showed catabolite repression, but enzyme production was induced by cellobiose, CMC, and starch, indicating that
-glucosidase is inducible enzyme. Yeast extract stimulated
-glucosidase production more than peptone and ammonium sulfate.
-Glucosidase activity was increased with 50mM MgCl
in 10mM potassium phosphate buffer (pH 7.0). Optimum conditions for enzyme activities were pH 6.0 and 42
, Km value of
-glucosidase for p-nitrophenyl-
-D-glucosidase was 0.256mM and Ki for
-D(+)-glucose was 9.0mM.
Cloning of Promoters from Alkali-tolerant Bacillus sp.
Microbiology and Biotechnology Letters, volume 16, issue 2, 1988, Pages 126~130
Promoters of an alkali-tolerant Bacillus sp. isolated from soil have been cloned in Bacillus subtilis using promoter probe vector pPL703. The CAT specific activity of a clone harboring the strongest promoter activity among these transformants was 8.01. This activity was 2.5 times higher than that of Bacillus subtilis harboring expression vector pPL708 and was increased after the end of the logarithmic growth phase. In the 2.8kb of inserted DNA fragment, BamHI and Sal I recognition sites were located.
Study on Mixed Cultures of Lactobacillus acidophilus and Saccharomyces cerevisiae in Soymilk
Microbiology and Biotechnology Letters, volume 16, issue 2, 1988, Pages 131~135
Lactobacillus acidophilus KFCC12731 and Saccharomyces cerevisiae KFCC32017 were incubated together in soymilk and the conditions for acid production were investigated. The acid production of Lactobacillus acidophilus was much higher when this organism was incubated with Saccharomyces cerevisiae in soymilk than when it was incubated alone. Optimum acid production by the mixed cultures of Lactobacillus acidophilus and Saccharomyces cerevisiae was achieved with the following conditions; a temperature of 34
, a 3:7-8:2 (OD 660) ratio of Lactobacillus acidophilus to Saccharomyces cerevisiae at inoculum, a 1.5% level of sucrose fortification or a 2.0-3.0 % level of skim milk powder fortification and a culture time of 12 hours or more.
Susceptibility of Saccharomyces cerevisiae D-71 and Zygosaccharomyces rouxii SR-S to Zymolyase-20T
Microbiology and Biotechnology Letters, volume 16, issue 2, 1988, Pages 136~141
Susceptibility of a thermophilic strain (D-71) of Saccharomyces cerevisiae and an osmotolerant strain (SR-S) of Zygosaccharomyces rouxii to Zymolyase-20T were studied in various renditions. Content of glucan and mannan in cell wall of Saocharomyces cerevisiae D-71 were 14.5% and 14.8%, and Zygosaccharomyces rouxii SR-S were 24.0% and 19.0%, respectively. Susceptibility of Saccharomyces cerevisiae D-71 cultured in Wickerham synthetic medium containing 0.5% of methionine and 0.1% of glucose to Zymolyase-20T was 66%, and
and aminobenzoic acid were greatly effective to susceptibility. Susceptibility of Zygosaccharomyces rouxii SR-S cultured in Wickerhnin synthetic medium containing 0.5% of peptone, 0.15% of methionine and 0.l% of glucose to Zymolyase-20T was 80%, and KI and pyridoxine were greatly effective to susceptibility. Susceptibility of Saccharomyces cerevisiae D-71 stationary cultured in YMPG medium at
for 12 hours was 16o1e and Zygosaccharomyces rouxii SR-S stationary cultured in YMPG medium at
for 30 hours was 82%.
Protoplast Formation and Fusion between Saccharomyces cerevisiae D-71 and Zygosaccharomyces rouxii SR-S
Microbiology and Biotechnology Letters, volume 16, issue 2, 1988, Pages 142~149
This experiment was carried out to obtain a hybrid with potent ethanol fermenting ability, by means of protoplast fusion between a thermophilic strain (D-71) of Saccharomyces cerevisiae and an osmotolerant strain (SR-S) of Zygosaccharomyces rouxii. The conditions for formation of protoplasts from both strains and for their fusion and regeneration were studied. Favorable conditions for formation of protoplasts from Saccharomyces cerevisiae D-71 were : treatment of the cells at late-exponential phase with 2-mercaptoethanol (l% v/v) for 10 minutes in the presence of 0.5M sorbitol, then incubation for 60 minutes in the set medium containing Zymolyase-20T (4mg/
) ; and from Zygosaccharomyces rouxii SR-S were : treatment of the cells at mid-exponential phase with 2-mercaptoethanol (1% v/v) for 10 minutes in the presence of 0.5M or 1M mannitol, then incubation for 120 minutes in the set medium containing Zymolyase-20T(4mg/
). The protoplasts of parental cells were fused in the presence of 20mM CaCl
, 0.5M sorbitol and 40% of polyethyleneglycol (M.W 4000), then fusants obtained were selected as regenerated colonies which embedded and grown in the minimal medium containing 3% of agar. The frequencies of fusant formation were 1.2
for the regenerated protoplast.
Development of L-Lysine Producing Strains from Cellulosic Substrate by the Intergeneric Protoplast Fusion- Conditions for Formation and Regeneration of Protoplast -
Microbiology and Biotechnology Letters, volume 16, issue 2, 1988, Pages 150~155
In order to produce L-lysine from cellulosic substrates by the intergeneric protoplast fusion between cellulolytic bacteria, Cellulomonas flavigena KFCC31221 and amino acid producing bacteria, Brevibacterium flavum ATCC14067, Corynebacteriurn glutamicum ATCC13032, conditions for protoplast formation and regeneration of these strains were investigated. After the strains were mutated with 500
N-methyl-N'-nitro N-nitrosoguanidine for 30 min and the mutants were enriched by treating 300
penicillin-G for 2 hrs, B. flavum Hse- Str
, C. glutamicum Met
and Cellulomonas flavigena Thr
were isolated. The rate of protoplast formation ranged from 95 to 98% when strains were treated at the concentration of 500
of lysozyme, pH 6.5, 33
, for 6 hrs. in Tris- malate buffer supplemented with 0.4M sucrose as osmotic stabilizer. Approximately 30-33% protoplast was regenerated on the regeneration complete medium(RCM) containing 1.5% agar and 0.5M sodium succinate overlaid with the same medium except 0.7% agar.
Formation and Regeneration of Penicillium verruculosum Protoplasts
Chung, Ki-Chul ; Park, Chang-Ryeol ;
Microbiology and Biotechnology Letters, volume 16, issue 2, 1988, Pages 156~162
Optimal conditions for the formation and regeneration of protoplasts of the cellulolytic fungus Penicillium verruculosum were investigated. Among the various commercial cell wall lytic enzymes tested, 0.5%(w/ v) Novozym 234 was the most effective for protoplast formation. The highest yield of protoplast exceeding 4.5
obtained when 400mg of 20 hr-old mycelia was incubated with 0.5%(w/v) Novozym 234 at 3
for 1 hr. The best osmotic stabilizer for the isolation and re-generation of protoplasts was 0.7M sorbital (pH 5.6) and 0.6M MgSO
(pH 5.6), respectively. When 0.6M MgSO
was added as osmotic stabilizer to the complete medium, the maximum regeneration frequency obtained was 4.6-27.8%. Micromorphological change of giant protoplasts into hyphae was observed during incubation in the regeneration liquid medium.
Intraspecific Protoplast Fusion of Cellulolytic Fungus, Penicillium verruculosum
Chung, Ki-Chul ; Park, Chang-Ryeol ; Suk Bai ; Chun, Soon-Bai ; Kim, Ki-Chung ;
Microbiology and Biotechnology Letters, volume 16, issue 2, 1988, Pages 163~167
The conditions for the protoplast fusion of auxotrophic mutants of Penicillium verruculosum were determined. A preparation of commercial enzyme Novozym 234 was used to successfully isolate protoplast from the 20hr old mycelium of P. verruculosum. Under optimal condition, the protoplast yield ranged from 2.4
protoplasts from 400mg of damp mycelia of various auxotrophic mutant strains. The regeneration frequency ranged from 26.6 to 42.4% and the spontaneous reversion frequency of the protoplasts on the regeneration minimal medium was less than 10
. The optimal concentration of PEG 6000 was 20%, and exposure of protoplasts to PEG for 10 min was found to be sufficient for protoplast fusion. Optimal pH of fusion mixture was deter-mined as 5.5 and l0mM of calcium chloride in fusion mixture effectively enhanced the protoplast fusion frequency. Under optimal condition, the fusion frequency between various auxotrophs ranged from 1.8
Immunological Analysis of Endotoxin Proteins Produced by Bacillus thuringiensis serovar. kurstaki HD1 and HA73
Microbiology and Biotechnology Letters, volume 16, issue 2, 1988, Pages 168~173
Immunological analysis between endotoxin proteins produced by Bacillus thuringiensis serovar. kurstaki HD1 and HD73 have been investigated by using polyclonal antibodies. The antisera against the endotoxin proteins were prepared from rabbits injected with the endotoxin protein antigens. When about 2mg/
of the antigens were injected for 7 times, the titers were highest. The stability of the antigens was reduced to about 50% after 9 days incubation at 4
. The sensitibity of endotoxin protein from B. thuringiensis HD1 and HD73 by indirect ELISA was 50ng/
, respectively. The cross reaction of antiserum appeared that anti-HD1 partialy reacted with crystal protein but anti-HD73 reacted with HD1 endotoxin about 100%.